• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.118-118
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• 2018

인삼은 우리나라에서 오랜 역사동안 많은 연구가 진행되어 왔으며, 현재는 다양한 방법으로 홍삼과 흑삼으로 만들어 식품, 화장품, 의약품 등 다양한 방면으로 사용하고 있다. 본 연구에서 시중에서 구매한 새싹삼(인삼새싹) 잎/줄기에 함유된 진세노사이드(Re, Rg1, Rb1, Rg3, Rh1) 함량을 높이기 위하여 증숙과 초음파 추출조건에 관한 연구를 수행하여 우수한 항노화 소재를 개발하기 위하여 실시하였다. 실험은 새싹삼 잎/줄기를 증숙 온도와 시간의 조건에서 진세노사이드 함량이 가장 높은 조건을 선정하였으며, 선정된 조건의 새싹삼 잎/줄기에 파장과 출력에 대한 조건으로 초음파 추출을 진행하여 진세노사이드가 가장 높은 함량을 보이는 조건을 선정하였다. 그 결과 새싹삼 잎/줄기추출물(GSE; Ginseng Sprout Extract)의 진세노사이드 함량은 4.8 mg/g으로 확인되었으나 증숙공정을 통해 8.82 mg/g으로 함량이 증가되었으며, 상기 증숙된 새싹삼 잎/줄기에 초음파공정을 적용하여 추출한 새싹삼 잎/줄기초음파추출물(SU-GSE; Steaming & dry Ultrasonication-Ginseng Sprout Extract)에서는 최대 10.65 mg/g으로 함량이 증가되었다. 반면, 새싹삼 뿌리의 진세노사이드는 2.30 mg/g으로 확인되었으나 증숙공정을 통해 4.95 mg/g으로 함량이 증가되었으며, 초음파추출공정을 통해 최대 5.82 mg/g으로 함량이 증가된 것을 확인할 수 있었으나, 새싹삼 잎/줄기에 비해 진세노사이드 함량이 낮은 것을 확인하였다. 항노화 소재로의 활용가능성을 평가하기 위하여 새싹삼 잎/줄기추출물 GSE와 SU-GSE에 대한 세포생존률, 항산화 및 항노화에 대한 효능평가를 진행하였으며 GSE의 경우 $100{\mu}g/ml$에서 세포생존률이 82.4%를 보인 반면 SU-GSE에서는 $1,000{\mu}g/ml$의 농도에서 101.8%의 세포 생존률을 보였다. 항산화 활성의 경우 GSE와 SU-GSE $100{\mu}g/ml$ 농도에서 각각 52%와 81%의 항산화 활성을 나타냄으로써 SU-GES의 조건에서 항산화 활성이 우수한 것으로 확인되었다. 또한, 항노화 활성에 대한 실험결과 MMP-1 유전자 발현에 대한 억제율을 비교한 결과 GSE와 SU-GES $100{\mu}g/ml$의 농도에서 각각 18%와 29%의 억제율을 보임에 항노화 소재로의 활용가능성을 확인하였다.

• Korean Journal of Food Science and Technology
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• v.43 no.4
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• pp.490-494
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• 2011

Although bacterial outbreaks from ready-to-eat foods have increased, little information is available on microbial quality of sprouts in markets. Fifty sprouts and 30 salads were collected from wholesale markets. Total aerobic count (TAC), coliform, Escherichia coli, and some pathogens were detected. TAC for sprouts was 7.95 log CFU/g and 6.70 for salads, indicating that sprouts were more contaminated by 1 log CFU/g than that of salads. The numbers of coliform were 6.69 log CFU/g for sprouts and 5.42 for salads. E. coli was detected in 16 of 50 sprout samples at 2.38 log CFU/g and eight of 30 salads at 2.21 log CFU/g. Bacillus cereus was detected in 29 of 50 sprout samples and 16 of 30 salads, and the counts were mostly <3 log CFU/g. Salmonella, Staphylococcus aureus, Listeria monocytogenes, Campylobacter jejuni, and Clostridium perfringens were not detected. Therefore, although pathogens may not be a high risk for these foods, the high TAC and E. coli contamination require improved production and distribution methods, particularly for sprouts.

• KIPS Transactions on Computer and Communication Systems
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• v.5 no.4
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• pp.79-86
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• 2016

About 75% of software security incidents are caused by software vulnerability. In addition, the after-market repairing cost of the software is higher by more than 30 times than that in the design stage. In this background, the secure coding has been proposed as one of the ways to solve this kind of maintenance problems. Various institutions have addressed the weakness patterns of the standard software. A new Korean programming language Saesark has been proposed to resolve the security weakness on the language level. However, the previous study on Saesark can not resolve the security weakness caused by the API. This paper proposes a way to resolve the security weakness due to the API. It adopts a static analyzer inspecting dangerous methods. It classifies the dangerous methods of the API into two groups: the methods of using tainted data and those accepting in-flowing tainted data. It analyses the security weakness in four steps: searching for the dangerous methods, configuring a call graph, navigating a path between the method for in-flowing tainted data and that uses tainted data on the call graph, and reporting the security weakness detected. To measure the effectiveness of this method, two experiments have been performed on the new version of Saesark adopting the static analysis. The first experiment is the comparison of it with the previous version of Saesark according to the Java Secure Coding Guide. The second experiment is the comparison of the improved Saesark with FindBugs, a Java program vulnerability analysis tool. According to the result, the improved Saesark is 15% more safe than the previous version of Saesark and the F-measure of it 68%, which shows the improvement of 9% point compared to 59%, that of FindBugs.

• Journal of Bio-Environment Control
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• v.24 no.1
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• pp.45-50
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• 2015

Edible sprouts are highly nutritious, containing numerous health-promoting phytochemicals and nutrients. However, the process of sprouting is subject to potential contamination by microorganisms attached to the seed coat. The objective of this study was to determine the effects of air anions generated by spray ionization on growth and sterilization of edible sprouts. Treatment with air anions has positive effects on plant growth and sterilization in 4 kinds of sprouts. Hypocotyl length in red cabbage and kale increased approximately 1.26-fold and radicle length of lettuce, red cabbage, and kale increased 1.4 to 1.6-fold compared to the control. The fresh weight of sprouts in the spray-ionization treatment was significantly (16.0-38.5%) higher than that of the controls. Radicle activity in sprouts exposed to anions was higher than that of the control in all species. Bacterial counts on lettuce, red cabbage, and tatsoi decreased significantly (by 41%, 66%, and 19%, respectively), and bacterial colonies also declined in drainage water. Our findings suggest that spray-ionization treatment is useful for improving sprout growth and for sterilizing sprouts.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.79-79
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• 2020

본 연구는 새싹인삼(Panax ginseng sprout) 재배용으로 이용하기 위한 묘삼(종삼)의 장기 저온저장 시 저장온도가 묘삼의 새싹인삼으로의 생장과 품질에 미치는 영향을 조사하여 묘삼의 저장에 적합한 온도를 구명하고자 수행하였다. 2년생 묘삼을 50 ㎛ LDPE 필름에 100g씩 넣어 포장하였고 온도 0, -2, -4℃에서 10개월 동안 저장하면서 2개월 마다 꺼내어 5℃ 저장고로 이동하여 5~7일 온도순화처리를 거친 후 실험을 실시하였다. 1차 육안조사로 묘삼의 부패율과 2차 생육조사로 묘삼을 재식 후 새싹인삼으로 재배 후 생장 상태와 품질을 조사한 결과 저장 4개월 이후 묘삼의 생육조사 시 새싹인삼의 생장과 품질이 묘삼의 저장온도에 따라 유의적인 차이를 보였다. 즉, 저장 4개월 된 묘삼의 건전한 새싹인삼으로의 생장은 0℃ 저장 묘삼에서 58.3%, -2℃ 저장 묘삼에서 72.1%, -4℃ 저장 묘삼에서 37.2%로 조사되었고, 저장 8개월 된 묘삼은 0℃ 저장 묘삼에서 9.7%, -2℃ 저장 묘삼에서 54.3%, -4℃ 저장 묘삼에서 6.9%로 조사되었다. 특히 0℃ 저장 묘삼은 저장 2개월에 출아가 진행되었고 저장기간이 지날수록 출아된 싹이 동해의 피해를 받는 것으로 조사되었다. 묘삼의 생육특성인 엽장, 엽폭, 경장, 근장을 측정한 결과 -2℃에 저장된 묘삼의 엽폭과 경장의 길이가 유의적으로 긴 것으로 조사되어 새싹인삼 재배를 위한 묘삼의 적정 저장온도는 -2℃로 설정하였다.

• Proceedings of the Korea Contents Association Conference
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• 2016.05a
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• pp.47-48
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• 2016

최근 스마트폰, 게임, 자동차 인포테인먼트 등 많은 애플리케이션이 GUI 기반으로 제작된다. "새싹"은 영어가 약한 프로그래밍 초심자를 위한 한글 프로그래밍 언어이다. 하지만 "새싹"은 아직 한글 GUI 라이브러리는 지원하지 않는다. 따라서 본 논문은 한글 프로그래밍 언어인 "새싹"을 위한 한글 GUI API를 제안하고자 한다. 그리고 "새싹"을 이용한 간단한 계산기 GUI 프로그램 예제를 통해 프로그램을 구현한다. 그 결과 정상적으로 작동하는 것을 확인하였다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.12a
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• pp.77-77
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• 2020

본 연구에서는 비트 새싹 추출물의 항산화 및 항염증 효능을 평가하기 위해 3가지 품종의 레드 비트(메를린, 우단, 디트로이트다크레드) 새싹을 60% 에탄올로 추출한 후 free radical 소거활성, 총 폴리페놀 함량을 측정하였다. 또한, LPS로 염증반응이 유도된 대식세포인 RAW264.7 세포에서 nitric oxide(NO) 생성억제 효과를 알아보았다. 메를린, 우단, 디트로이트다크레드 새싹 추출물의 총 폴리페놀 함량을 querceitn을 표준물질로 사용하여 측정한 결과 2.84 mg Que/g, 2.61 mg Que/g, 2.71 mg Que/g으로 나타났고 DPPH 라디칼 소거능은 농도 의존적으로 증가하였으며, 200 ㎍/ml일 때, 55%, 72%, 63%로 나타났다. 또한, DPPH 라디칼 소거능이 가장 높은 메를린 새싹 추출물은 LPS로 염증반응이 유도된 RAW264.7 세포에서 농도 의존적으로 iNOS와 COX-2를 억제하는 것을 확인하였다. 이러한 결과를 바탕으로 메를린 비트 새싹 추출물이 항산화 및 항염증제의 천연물 소재로 활용이 가능할 것으로 생각된다.

• Korean Journal of Plant Resources
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• v.22 no.4
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• pp.304-311
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• 2009

This study was conducted to develop functional sprout vegetables with antioxidant effects using seeds of Arctium lappa. The seeds germinated vigorously under light at $25^{\circ}C$, reaching germination rate of 82% within 4 days. Germinated seeds were placed under darkness at various temperatures to force growth in length, and it was demonatrated that $20^{\circ}C$ was optimum temperature. Greening treatment reduced growth in length, but promoted growth of cotyledons. Harvested A. lappa sprout vegetables maintained freshness longer at $10^{\circ}C$, rather than $4^{\circ}C$. Ventilation holes in storage containers had no effects on storage periods. Antioxidant activity of vegetable that received greening treatment for 1-3 days was investigated, and it was shown that free radical scavenging effects and ferrous ion chelating effects was higher than those of commercially available brocoli, cauliflower, pea and bean sprout. Contents of total polyphenol and flavonoid were also higher, especially by 3 day greening. The longer the treatment, the more the inhibition on peroxidation of linoleic acid. Sprout vegetable of A. lappa had higher antioxidant activity compared with adult plant. In conclusion, sprout vegetable of A. lappa has great potentiality for use as one of sprout vegetables.

• Korean Journal of Organic Agriculture
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• v.25 no.2
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• pp.499-511
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• 2017

This study was done to examine antioxidant compounds, antioxidative activity and chemical properties in organically cultivated sorghum seed and their sprout, and to examine as a basic data for various application methods as an organic farming product of sorghum. The crude protein and mineral contents in the sprout had 2.5 and 1.6~9.87 folds more than sorghum seed, respectively. Total polyphenol content as antioxidant was more in the sprout showing $4,135mg\;GAE\;g^{-1}$ than the seed, whereas total flavonoid content was relatively higher in the seed showing $3.86mg\;GAE\;g^{-1}$. ABTS radical scavenging activity in the seed was observed by higher level as $15.78mg\;TE\;g^{-1}$. However, The sprouts with and without natural light were no significant difference. DPPH radical scavenging activity in the seed exhibited activity of 11 folds or more compared to that of the sprout. The highest activity was detected from the seeds and the sprouts in Donganmae. Although sorghum was no significant correlations among the varieties, it showed correlation with between the light-treated condition and antioxidation.

• The Journal of the Korea Contents Association
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• v.16 no.3
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• pp.288-295
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• 2016

As the computer becomes pervasive in everyday life, the programming arises as an essential technique. To support the education of programming, Republic of Korea has launched a plan to develop the official courses of programming by 2018. However, the programming education in Korea tends to be difficult and the students easily lose their concentration since most of programming languages are based on English. This paper proposes a Korean programming language called Saesark to promote the effective programming education for Korean students. Saesark has been developed based on Java, which supports the object-oriented programming and the lambda expressions. In order to evaluate the educational suitability of Saesark, we compared it with other Korean programming languages in three respects: syntactic features, IDE support, and error messages in Korean. According to the comparison, Saesark is revealed more suitable than other languages. Specifically, the IDE features and the error messages in Korean are expected to be mostly valuable in educating novice programmers.