• Title/Summary/Keyword: 살모넬라

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죽초액의 첨가가 육성오리의 생산성 및 육질에 미치는 영향

  • Kook, Gil;Lee, Jong-Bong;Lee, Jae-Il;Kim, Jae-Pil;Kim, Gwang-Hyeon
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2001.11a
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    • pp.80-82
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    • 2001
  • This experiment was conducted to investigate the effects of Bamboo Vinegar Liquid(BVL) on performance and meat quality of broiler ducks for 5 weeks. One hundred broiler ducks were divided into 4 groups with S replicates per treatment. Dietary levels of food waste 0%, 1%, 2% and 4% were included in experimental diets. The growth performance and carcass weight of broiler duck fed BVL supplement diets were not significantly different(P>0.05) from the control birds. The lipid content was decreased(P<0.n) by addition of BVL 2% and 4%, whereas the protein was increased(P<0.05). The cholesterol content and TBA in breast meat were significantly(P<0.05) decreased by addition of BVL. palmitic acid of fatty acid composition were decreased by addition BVL 2% and 4% whereas linoleic acid was increased by addition of BVI. 2% and 4%. The odor, appearance and taste of sensory evaluation were improved by addition BVL 1% and 2%. The results of this study indicate that dietary supplemental BVL 1% and 2% may improve the meat quality of broiler ducks.

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Antibacterial Activities of Medicinal Herbs on Salmonella and E. Coli (생약재가 살모넬라균 및 대장균의 항균에 미치는 효과)

  • Kim, Gye-Yeop;Nam, Ki-Won;Jang, Seung-Joo;Kim, Jung-Eun;Iim, Joung-Soo;Ahn, Byung-Sun;Kwun, Du-Seog;Jung, Hun-Woo;Cho, Kyoung-Oh
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.3
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    • pp.619-623
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    • 2008
  • The purpose of this study was to observe the effects of antibacterial activities on various medicinal herbs. Medicinal herbs used this study was Curcuma aromatica, Scutellaria baicalensis, Angelica dahurica, Platycodon grandiflorum, Houttuynia corolata, and Forsythia saxatilis. To evaluation of the antibacterial effect, we used S. gallinarum(ATCC 9184), S. typhimurium(SAL-13, France), S. enteritidis (ATCC 10376), Escherichia coli(E-62, O78), and S. gallinarum(E. coli). Extracts from each medicinal herbs divided into 100mg/ml, 50 mg/ml, 25 mg/ml, and 12.5 mg/ml. Antibacterial activities achieved by the paper disc test. Antibacterial activities was showed at extracts from Scutellaria baicalensis, Houttuynia corolata, Forsythia saxatilis, but little found at Curcuma aromatica, Angelica dahurica, Platycodon grandiflorum.

Epidemiologic Investigation on an Outbreak of Salmonellosis in Yeongcheon-si, 2004 (2004년 영천시 D 초등학교에서 집단 발생한 살모넬라증)

  • Min, Young-Sun;Lee, Hwan-Seok;Lim, Hyun-Sul
    • Journal of Preventive Medicine and Public Health
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    • v.38 no.4
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    • pp.457-464
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    • 2005
  • Objectives : An outbreak of salmonellosis occurred among the student s and staff of D primary school in Yeongcheon-si, 2004. This investigation was carried out to prevent any recurrence of this outbreak and to study the infection source and transmission of the salmonellosis. Methods : The authors conducted a questionnaire survey among 1,205 students and staff members from D primary school about the ingestion of the school lunch and drinking water, and the manifestation of their symptoms. The author examined rectal swabs, the tap water and microorganism cultures, and we also investigated the dining facility and water supply facility. Results : The diarrheal cases were defined as the confirmed cases or the persons who had diarrhea more than one time with symptoms such as fever, vomiting and tenesmus. The diarrheal attack rate was 28.0%. Ingestion of fried bean curd with egg had a significantly high association with the diarrheal attack rate (p<0.05), and the relative risk of the fried bean curd with egg was 10.68 (95% CI=3.88-29.41), as was determined by logistic regression analysis. The bacterial counts in the tap water of the food preparation room and toilet (first floor) exceeded the maximum permissible counts. S. Enteritidis bacteria were only cultured from the fried bean curd with egg of all the supplied foods on September 3, 2004. Conclusions : The major cause of salmonellosis was presumed to be the contaminated bean curd via contaminated tap water due to a water leak of a school water pipe. This contaminated bean curd was under prepared, which allowed the S. Enteritidis to survive and multiply prior to its ingestion.

Right Pulmonary Artery Agenesis -A Case Report- (우측 폐동맥 형성부전증 -수술치험 1례-)

  • Sin, Dong-Geun;Kim, Min-Ho;Kim, Gong-Su
    • Journal of Chest Surgery
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    • v.30 no.1
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    • pp.108-111
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    • 1997
  • Congenital unilateral agenesis of pulmonary artery is a rare anomaly and it usually occurs in association with other cardiac anomaly such as tetralogy of Fallot. Since most patients affected by this defect without associated congenital cardiac anomaly or pulmonary Infection are asymptomatic, the clinical diagnosis of this anomal is first recognized by a characteristic pattern in chest roentgenogram taken as a routine checking; the findings on chest film consists of cardiac and mediastinal displacement, absence of the pulmonary arterial shadow, smaller hemithorax, and elevationof the hemidiaphragm, all on the affected side. We experienced rlght pulmonary artery agenesis in a 48 year-old male, who complained of massive hemoptysis, and it was diagnosed by digital subtraction pulmonary arteriogram and perfusin scan, and treated by right middle and lower lobe bi-lobectomy, and we report this case with the review of relevant literatures.

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Effect on Livestock Manure Composting by the Enriched Microbial Population (미생물에 의한 축산 폐기물 퇴비화에 미치는 영향)

  • 신혜자
    • Journal of Life Science
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    • v.12 no.2
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    • pp.129-135
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    • 2002
  • Several kinds of thermophilic, aerobic microorganisms (Bacillus genus), metal leaching microorganisms (Thiobacillus, T. ferooxidans), and other nondegradable chemical-degrading microorganisms (Pseudomonas genus) were utilized to study the effect on composting livestock manure. Under the Carbon-Nitrogen ratio (C/N) of 35∼40 and water content of 50∼65% conditions, the composting in the cycling drum reactor showed slower composting and lower temperature increase than that of the manual reactor. Element analysis after composting indicated relatively high levels of mineral contents with the substitutional effect of chemical fertilizer. Metal analysis before and after composting showed lower As in all, Cr in pig, Pb in cow, Hg in chicken, and Cu in cow manure compost than the regulation values. Compost maturity was ascertained by the several maturity tests. Salmonella and E. cozi detection test by SS or EMB agar plate confirmed the safety from the pathogenic microorganisms. The results suggest that the inoculation of metal and some other chemical degrading microorganisms during composting might decrease metal contamination and increase composting rate.

Analysis of Microbiological Contamination in Ready-to-eat Foods (즉석 섭취 식품에 대한 미생물 오염 분석)

  • Kim, Ha-Kyu;Lee, Hak-Tae;Kim, Jong-Ho;Lee, Sang-Sun
    • Journal of Food Hygiene and Safety
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    • v.23 no.4
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    • pp.285-290
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    • 2008
  • This study was carried out to examine microbiological contamination of ready-to-eat foods (kimbab, sushi, salad, sandwich, sashimi) and to prove hazard of ready-to-eat foods by microbiological analysis. Collection of 440 samples of ready- to-eat foods were obtained from department stores, discount stores, super-markets in Seoul, Gyeonggi, Chungcheong, Gyeongsang, Honam areas. Sushi showed the highest detection rate of micro organism with value of20.4%, and then detection rates of kimbab, sashimi and salad were 13.0%, 12.5% and 6.9%, respectively. S. aureus was the most detected microorganism with value of 6.6%, and then Coliform and L. monocytogenes were detected 2.7% and 2.3%.

Evaluation of antibacterial and therapeutic effects of egg-white lysozyme against Salmonella Typhimurium in ICR mice infected with Salmonella Typhimurium (난백 유래 라이소자임의 마우스 살모넬라증에 대한 항균 및 치료 효과)

  • Kim, Hee-Gyu;Hwang, Jae-Seung;Jae, Woo-Young;Son, Song-Ee;Lee, Hu-Jang
    • Korean Journal of Veterinary Research
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    • v.56 no.2
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    • pp.103-108
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    • 2016
  • Salmonellosis is a major bacterial zoonosis that causes self-limited enteritis in animals and foodborne disease and typhoid fever in humans. Recently, multi-drug-resistant strains of Salmonella spp. have increased and caused more serious problems in public health. The present study investigated the antibacterial effects of egg-white lysozyme (EWL) against Salmonella (S.) Typhimurium and the therapeutic effects of EWL for murine salmonellosis. Evaluation of the antibacterial effects of EWL against S. Typhimurium revealed a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of EWL of 6.25 and $300{\mu}g/mL$, respectively. In the bacterial growth inhibition test, EWL at 300 (p < 0.05) and $600{\mu}g/mL$ (p < 0.01) significantly inhibited the growth of S. Typhimurium at 4 h post-incubation. EWL administration at MIC (LYS-1), MBC (LYS-2) and $2{\times}MBC$ (LYS-3) for 14 days resulted in mortality of mice infected with S. Typhimurium of 70, 40 and 10%, respectively, while that of control mice (CON) was 90%. Counts of S. Typhimurium in murine spleens were significantly lower in LYS-2 and LYS-3 than CON (p < 0.05). The results of this study indicate that EWL has the potential for treatment of ICR mice infected with S. Typhimurium.

Identification of Salmonella Pathogen Using Electronic Nose (전자코를 이용한 살모넬라 식중독균 판정)

  • Kim G.;Lee M. W.;Lee K. J.;Choi C. H.;Noh K. M.;Kang S,;Chang Y. C.
    • Journal of Biosystems Engineering
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    • v.30 no.2 s.109
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    • pp.121-126
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    • 2005
  • In this study, a commercial electronic nose system was used to detect contamination of Salmonella bacteria. Odors from growth media contaminated with Salmonella typhimurium, Salmonella enteritidis, or Escherichia coli were collected and analyzed to evaluate a possibility of rapid detection of pathogen. Odor chromatograph showed that S. typhimurium, S. enteritidis, and E. coli had 7,6, and 9 main peaks, respectively. Retention time and intensity of the peaks were distinct for different bacteria species. Principal component analysis (PCA) were also performed to clarify odor differences. Analysis results showed that the odors for uncontaminated growth medium were differently grouped from the odors of contaminated one. The odor from the bacteria growth identified with two principal components, PC 1 and PC2. In PCA figures, odor groups were moved from left to right of PC 1 with elapse of the bacteria growth time. The electronic nose system could detect odors of S. typhimurium, S. enteritidis, E. coli when their concentration were $1.85\times10^6\;cfu/g,\;2.25\times10^6\;cfu/g,\;and\;1.8\times10^5 cfu/g$, respectively.

An Improved Method for Detection of Salmonella Typhi O Antigen with Staphylococcal Protein A Using Enzyme Immunoassay (포도구균의 A단백질을 이용한 효소면역법으로 살모넬라 O항원 검출)

  • Rhyu, Mun-Gan;Kim, Gum-Ryong;Lee, Choong-Ki
    • The Journal of the Korean Society for Microbiology
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    • v.22 no.4
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    • pp.445-451
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    • 1987
  • Coagglutination method is widely used for the diagnosis of Salmonella infection. This test, however, has a disadvantage of false positive reaction due to the coagglutination of staphylococci with non-specific immune complexes or anti-staphylococci antibody in serum. Salmonell O antigen was detected by enzyme immunoassay with protein A-bearing Staphylococcus aureus as in the solid phase. Horse radish peroxidase was labeled to IgG specific against Salmonella O antigen. This enzyme immunoassay was much more sensitive than conventional coagglutination method without false poitive agglutination. To improve the sensitivity for detection of Salmonella O antigen in samples, we tried to determine the optimal concentration of normal IgG that inhibits non-specific binding of horse radish peroxidase labeled IgG to staphylococci, and to establish the optimal condition of reaction between antigen-antibody complex and staphylococci. Non-specific binding of horse radish peroxidase labeled specific IgG to staphylococci was almost blocked when the enzyme labeled IgG was 500-fold diluted with phosphate buffered saline containing 2mg/ml of normal IgG. When staphylococci coated with antibody to Salmonella O antigen were mixed with antigen-antibody complex and then incubated for 1 hour at room temperature, the minimal detectable concentration of Salmonella O antigen was 1ng/ml. The sensitivity of enzyme immunoassay was 100-fold greater than a conventional coagglutination method. This enzyme immunoassay could be expected as an improved method for detection of other infectious agents.

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Antimicrobial Activity of Citral against Salmonella Typhimurium and Staphylococcus aureus (살모넬라와 황색포도상구균에 대한 시트랄의 항균효과)

  • Kim, Jung-Jee;In, Ye-Won;Oh, Se-Wook
    • Korean Journal of Food Science and Technology
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    • v.43 no.6
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    • pp.791-794
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    • 2011
  • The aim of this study was to investigate the antimicrobial characteristics of citral against Salmonella Typhimurium and Staphylococcus aureus. Antimicrobial activities were determined according to the citral concentration and initial pH. The tested citral concentrations were 0-1,000 ppm in tryptic soy broth (TSB) and 0-5,000 ppm in Angelica keiskei juice (NokJeup). The initial pHs tested were 4-7. Antimicrobial activities increased as citral concentration increased. S. aureus was more susceptible than S. Typhimurium during culture in TSB. But S. aureus was less susceptible to pH changes. Citral caused about 1-2 log reduction of S. aureus and 2-5 log reduction of S. Typhimurium after 10 min exposure at different pHs. As the citral concentration in the Algelica keiskei juice increased, S. aureus was easily inactivated but S. Typhimurium was not inactivated.