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Bacterial Leaf Spot and Dry Rot of Lettuce Caused by Xanthomonas campestris pv. vitians (Xanthomonas campestris pv. vitians에 의한 상추 세균성점무늬병)

  • Lee, Seung-Don;Lee, Jung-Hee;Kim, Yong-Ki;Heu, Sung-Gi;Ra, Dong-Soo
    • Research in Plant Disease
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    • v.13 no.1
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    • pp.66-70
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    • 2007
  • During 1997 and 1998, a new disease of lettuce (Lactuca sativa) was observed on greenhouse-grown plants in Kwangju and Kwangmyung. Lesions on leaves were irregular, small, pale green to black, water-soaked, and 2 to 5 mm in diameter, Coalescing lesions sometimes caused defoliation of older leaves. Isolations made from diseased leaves on yeast extract dextrose calcium carbonate agar yielded nearly pure cultures of a yellow pigmented bacterium typical of a xanthomonad. Two bacterial strains (SL0246 and SL1352) were purified and used for further tests. Pathogenicity of strains was confirmed on 5-week-old lettuce plants injected with bacterial suspensions containing $10^8$ cfu/ml of phosphate buffered saline. The representative Xanthomonas strains isolated from lettuce were compared with a reference strain X. campestris pv, vitians for fatty acid profiles and metabolic fingerprints using GN2 microplate, showing that all outcomes were indistinguishable between the representative and reference strains. This is the first report of bacterial leaf spot and dry rot of lettuce in Korea.

남해 동부 연안 지역 지하수의 수리지구화학적 연구: 해수침투에 대한 고찰

  • 신광섭;윤성택;허철호;이상규
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.04a
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    • pp.438-441
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    • 2003
  • 남해 동부 연안 지역 지하수의 해수 침투 영향을 광역적으로 파악하기 위하여, 202개소의 지하수 시료를 채취하고 수리지구화학적 연구를 수행하였다. 전체 시료 중 14.4%가 500 $\mu\textrm{m}$/cm을 초과하는 높은 EC값을 나타내어 해수 영향을 시사해 주었다. 해수 영향 판단의 또 다른 파라메터로 사용되는 CI/HCO$_3$ 몰비는 전체 시료의 23.3%가 1.3 이상의 값을 나타내었다. 한편, 대표적으로 해수침투 영향 및 인위적 오염의 영향을 각각 반영하는 것으로 알려진 Cl과 NO$_3$의 농도에 관한 누적도수분포도를 작성하여 배경수질군과 오염영향군을 구분짓는 배경치(background concentration)를 구한 결과, 각각 Cl = 22.3 mg/1 및 NO$_3$= 23.1 mg/l로 나타났다. 이 두 파라메터의 농도 분포를 기준으로 하여 채취된 지하수 시료를 크게 4개의 그룹으로 나눌 수 있었다. 그 결과, 자연적 또는 인위적 기원의 오염이 배제된 그룹 1은 전체 시료의 31%, 자연적 오염(해수 영향)을 반영하는 그룹 2는 전체의 24%, 인위적 오염의 영향을 반영하는 그룹 3은 전체의 9%, 해수 영향 및 인위적 오염을 동시에 반영하는 그룹4는 전체의 36%로 나타났다. 또한 그룹2와 그룹4는 대부분 Na-Cl유형으로 진화하는 Ca-Cl유형을 나타내었다. 따라서, 여러 지구화학 방법에 의한 해수 영향 판단 결과는 서로 잘 일치하고 있음을 알 수 있다. 결국, 지구화학적 파라메터를 종합적으로 활용함으로써, 연안 지역 해수침투의 효과적인 파악은 물론 예측 및 방지, 복구에 효과적으로 사용될 수 있을 것으로 판단된다.으로 토양에서 유동 가능성이 있는 중금속만을 추출하였다. 분석실험은 토양의 Cd2+ 와 Pb2+를 대상으로 행하여졌으며, 여러 토양에서 추출 분석한 결과를 EDTA분석결과와 비교하였다. 실험결과, 중금속은 매우 신속하게 고분자 자성체와 결합하였고, 그 후 자성체를 외부 자장으로 모은 후 산으로 용해시키고, 결합된 중금속은 Graphite furnace AAS로 분석함으로써 빠르고 효율적으로 분석실험을 수행할 수 있음을 알 수 있었다. 한편, 실험에서 나타난 수치들을 비교 검토한 결과 토양 분석시 sandy soil에서는 자성체를 이용한 분석이 EDTA에 의한 방법보다 더 높은 추출도를 보인 반면, silt 함량이 많은 토양의 경우에서 EDTA분석에서 더 높은 중금속 추출도를 보였다.s 중에서 490nm와 555nm의 복합밴드를 포함하는 OC2 알고리즘(ocean color chlorophyll 2 algorithm)을 사용하는 것이 OC2 series 및 OC4 알고리즘보다 좋은 추정 값을 도출할 수 있을 것으로 기대된다.환경에서는 5일에서 7월에 주로 이 충체의 유충이 발육되고 전파되는 것으로 추측되었다.러 가지 방법들을 적극 적용하여 금후 검토해볼 필요가 있을 것이다.잡은 전혀 삭과가 형성되지 않았다. 이 결과는 종간 교잡종을 자방친으로 하고 그 자방친의 화분친을 사용할 때만 교잡이 이루어지고 있음을 나타내고 있다. 따라서 여교잡을 통한 종간잡종 품종육성 활용방안을 금후 적극 확대 검토해야 할 것이다하였다.함을 보이고 있다.X> , ZnCl$_{3}$$^{-}$같은 이온과 MgCl$^{+}$, MgCl$_{2}$같은 이온종을 형성하기 때문인것 같다. 한편 어

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The Contamination of Groundwater by Acid Mine Drainage in the Vicinity of the Hanchang Coal Mine and the Efficiency of the Passive Treatment System (산성광산배수에 의한 한창탄광 부근 지하수의 오염과 자연정화처리시설 효율에 관한 연구)

  • 지상우;김선준
    • Journal of Soil and Groundwater Environment
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    • v.8 no.2
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    • pp.9-18
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    • 2003
  • Sampling of waters from each stage of treatment system (Successive Alkalinity Producing System; SAPS), tailings seepage, and spring near the Hanchang coal mine of Kangwon Province were carried out seasonally and analyzed to evaluate the source and possible path of groundwater contamination by acid mine drainage (AM). Sulfur isotope compositions were measured to identify the origin of groundwater contaminations and the sulfate reduction processes in the SAPS. Low pH and high metal concentration of spring water indicates possibility of the groundwater contamination by AMD. Removal efficiency of acidity of the SAPS was 18.17 g/$\textrm{m}^2$/day on an average and the metal removal efficiency was almost 100%, which was higher than those of other treatment systems. However, no appreciable decrease of sulfur content and almost similar sulfur isotope compositions of water from each stage of the treatment system may suggest incomplete or very poor sulfate reduction by sulfate reducing bacteria. Chemical and sulfur isotope compositions showed that spring water was contaminated by seepage from mine tailings. And seepage of stonewall, a part of treatment system was affected by both tailings seepage and mine adit drainage. In this study site, the treatment system was constructed for the only AMD from mine adit not for tailings seepages, which resulted in the groundwater contamination from tailing seepages. Similar situation is expected in other abandoned coal mine areas.

Physiological and Ecological Characteristics of Indigenous Soybean Rhizobia Distributed in Korea -I. Relationship between Distribution of the Indigenous Rhizobia and Physico-Chemical Properties (우리나라 토착대두근류균(土着大豆根瘤菌)의 분포상태(分布狀態)와 생리(生理) 및 생태학적(生態學的) 특성(特性) -제(第) 1 보(報). 토착근류균(土着根瘤菌)의 분포상태(分布狀態)와 토양특성(土壤特性)과의 상호관계(相互關係))

  • Ryu, Jin-Chang;Lee, Seong-Jae;Suh, Jang-Sun;Cho, Moo-Je
    • Korean Journal of Soil Science and Fertilizer
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    • v.19 no.1
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    • pp.38-49
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    • 1986
  • In order to improve effectiveness of rhizobia-legume symbiotic nitrogen fixation, ecological and physiological characteristics of indigenous rhizobia distributed in Korea soil, that is, the effects of soybean cultivation, physico-chemical properties and climate on the population of indigenous rhizobia and other soil microbes were investigated. The results were summarized as follows: The population of indigenous rhizobia were ranged from $5.1{\times}10^4$ cells to $196.8{\times}10^4$ cells per gram of soil in soybean cultivated soils but from $1.6{\times}10^4$ cells to $78.6{\times}10^4$ cells per gram of soil in soybean un-cultivated soils sampled from 9 different agro-climate zone. The highest population was observed in a soybean cultivated loamy soil from southern part of Korea. The content of available phosphate, exchangeable Ca, Mg, Cu, and B in soil were positively correlated but active Fe, exchangeable Al, Na, and $SO_4$ were inversely correlated to the population of indigenous rhizobia. The inverse relationship was observed between the number of indigenous rhizobia and actinomycetes.

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Trend analysis of Domestic water Consumption Depending upon the characteristics of using tap water and economical parameters (수돗물 사용특성과 경제적 요인에 따른 가정용수 소비 경향의 분석)

  • Choi, Sun-Hee;Kim, Sang-Hyun
    • Proceedings of the Korea Water Resources Association Conference
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    • 2007.05a
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    • pp.121-125
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    • 2007
  • 물 수요예측을 실시함에 있어서 사회 경제의 장래동향과 도시의 특성 및 발전 동향을 반영하여 수행하여야 한다. 그러나 지금까지의 관행으로는 수도계획에서 사용할 수 있는 실사용량에 대한 조사와 통계가 이루어지지 않고 있다. 실제 현장에서 얻은 자료를 토대로 하여 수도계획 및 설계에 사용할 수 있는 신뢰성 있는 설계인자의 도출이 필요하다. 본 연구에서는 급수지역의 각 조사 가정의 물 사용량을 실측 조사하여 얻어진 자료를 토대로 하여 물 수요 구조의 변화를 파악할 수 있는 수단의 하나로 가정의 수돗물 사용특성과 경제적 요인에 따른 가정용수의 사용특성 경향을 파악하고자 하였다. 가정에서 사용된 용수량의 조건별 경향성을 분석하기 위하여 한국수자원공사에서 2002년부터 2006년까지 3년여에 걸쳐 표본 집단이 되는 전국 140개 가구에 유량계를 설치하여 유량 자료를 획득하였고, 설문조사를 실시하여 각 가정의 물 사용 특성인자들을 조사하였다. 이 자료를 대상으로 비교적 자료의 신뢰성을 높이기 위한 자료의 선별과정을 거쳤다. 이렇게 선별된 자료들을 경향성 분석에 쓰이는 Mann-Kendall test와 Spearman's Rho test를 이용하여 분석하였다. 그 결과를 바탕으로 가정 용수 소비패턴의 증가 감소에 영향을 끼치는 인자들을 추출하였다. 실측자료를 분석을 통하여 나타난 결과들을 바탕으로 용수 수요처의 조건별 특성을 분석하고, 이를 활용한 생활용수 예측모형을 개발함으로써 합리적인 수요예측에 의한 용수수요의 과다예측 우려를 해소하고, 경제적 수도시설계획을 수립하는 등 과학적 물 수요관리 정책 수립을 위한 의사결정도구가 제공될 수 있다.c}$C의 저온에서 저장한 감자는 $20^{\circ}$C에서 저장한 감자보다 발아의 지연과 함께 낮은 PGA증가율을 보였다.다 높았으며, 전반적인 선호도의 경우 G3(1.5%)를 가장 선호하는 것으로 나타났다. 백년초 분말 첨가 도토리묵의 경우 색상은 0.5% 첨가한 O1이 가장 높은 값을 나타냈으며, 외관은 1.5% 첨가한 O3가 가장 높은 값을 보였다. 향미와 신맛의 경우는 백년초 분말의 첨가량이 증가됨에 따라서 유의적으로 증가했다(p<0.001). 씹힘성, 탄력성, 견고성의 경우는 대조군이 가장 높았으며, 백년초 분말 첨가량이 증가함에 따라 유의적으로 낮아졌다(p<0.001), 떫은맛의 경우는 백년초 분말에 의한 신맛의 영향으로 1% 백년초 분말이 첨가된 O2 가장 높게 나타났다. 전반적인 선호도는 0.5% 백년초 분말이 첨가된 O1이 가장 높게 나타났다. 따라서 녹차와 백년초의 기능성을 살린 도토리묵을 실용화시키는 효과적인 배합비는 녹차 분말 1.5% 첨가와 백년초 분말 첨가 0.5%가 바람직한 것으로 보이며 백년초 분말은 1%까지도 가능한 것으로 나타났다. 그러므로 기능성 식품 소재로써 도토리묵에 녹차와 백년초 분말을 첨가하는 것은 충분히 활용할만한 가치가 있다고 사료된다.론적으로, 비육돈 사료 내 3.32%의 호맥 사일리지의 혼합급여는 혈액 내 코티졸 함량, 도체육의 명도와 황색도, 지방산 조성 및 영양소 소화율에 영향을 미치는 것으로 사료되나 이에 대한 보다 많은 연구가 필요할 것으로 판단된다.니티와 공원과의 관계로 공원 설계와 관리에 있어서 영국에서는 커뮤니티가 직접 고객(client)으로서 역

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Applied-mineralogical Characterization for the Quick-lime Manufactured from Fine-grained Domestic Limestones (국내산 세립질 석회석으로부터 제조된 생석회에 대한 응용광물학적 특성 평가)

  • Noh, Jin-Hwan;Lee, Hyun-Chul
    • Journal of the Mineralogical Society of Korea
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    • v.22 no.3
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    • pp.261-277
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    • 2009
  • This study is aimed to emphasize the significance of ore selection in lime manufacturing through the evaluation of applied-mineralogical impact factors of crude ores controlling calcination characteristics for some domestic limestones used currently for lime manufacturing. To do this work, systematic characterization and determination were carried out for the limestone ores and their calcination products in a fixed calcining condition (target temperature: $1000^{\circ}C$, retention time: 30 minutes, 2, 4, 10, 16 hours), and the results were correlated and discussed. Selected high-Ca limestones in this study are as much as > 98 wt%, but they are somewhat diverse in crystallinity, texture, and impurity composition. Synthesized quicklimes are varied depending on such a difference in ore characters. The Pungchon limestone has relatively very low calcination rate, and the limestones from the Gabsan formation and the Jeongseon formation exhibit good quality in calcination rate and decrepitation. Among these samples, the limestone ore from the Jeongseon formation is evaluated to be the best for crude ore in manufacturing of highly-reactive quicklime. In addition, it is characteristic that the Gabsan limestone comparative rich in Fe-bearing mineral such as pyrite and goethite is more conspicuous in sintering effect.

Changes in Taste Compounds of Seasoned Pork with Korean Traditional Sauces during Aging (전통 장류로 제조한 양념육의 숙성 중 맛성분 변화)

  • Hah, K.H.;Joo, S.T.;Park, G.B.;Sung, N.J.;Lyou, H.J.;Park, K.H.;Kim, I.S.;Jin, S.K.
    • Journal of Animal Science and Technology
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    • v.47 no.5
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    • pp.857-866
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    • 2005
  • This study was carried out to evaluate changes in taste compounds of seasoned pork with Korean traditional sauces. The samples, pork loins were cut by the shape of cube($5{\time}15{\time}5$cm) and seasoned pork with Korean traditional sauces such as soy sauce base(T1), red pepper sauce base(T2), and soybean sauce base(T3) in the same proportion of meat seasonings, respectively. The seasoned samples were stored, at $1{\pm}1^{\circ}C$ during 28 days. Citric acid and fumaric acid were higher after 14 days of storage for the treatments, seasoned pork with red pepper sauce had higher compared to other treatments. While propionic acid and lactic acid were higher in seasoned pork with soybean sauce compared to other treatments. Free amino acids was higher in seasoned pork with soybean and red pepper sauce compared to seasoned pork with soy sauce. In all treatments, free amino acids was slightly increased with aging periods. 5'-inosine monophosphate(IMP) was decreased, whereas inosine was increased with increase of aging time. Inosine was higher in seasoned pork with soybean and red pepper sauce compared to seasoned pork with soy sauce. In sensory evaluation, aroma, flavor, tenderness and juiciness were increased with aging period increased in all treatments.

Screening for raw material of modified gelatin in marine animal skins caught in coastal offshore water in Korea (수식 어류껍질 젤라틴의 원료로서 연근해산 수산물껍질의 검색)

  • Cho, Soon-Yeong;Kim, Jin-Soo
    • Applied Biological Chemistry
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    • v.39 no.2
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    • pp.134-139
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    • 1996
  • In order to effectively utilize marine animal skin wastes in marine processing manufacture, conger eel skin, file fish skin and arrow squid skin as raw material of edible gelatin were screened. Conger eel skin was the highest in the collagen content, followed by Ole fish skin and arrow squid skin, in the order named. In the fish skins, the soluble and insoluble collagens occupied $67.4%{\sim}72.3%\;and\;27.7{\sim}32.6%$, respectively, and in the arrow squid skin, 30.4ft and 69.6ft, respectively. No difference in the amino acid composition between soluble and insoluble collagens was detected. Collagen from the marine animal skin catched in coasted and offshore water in Korea consisted ${\alpha}$ chain and ${\beta}$ chain, and ${\alpha}$ chain were hetero type. The sum of proline and hydroxyproline contents in conger eel skin collagen was higher than that in the other skin collagens, while was lower than that pork skin collagen. Conger eel skin collagen exhibited a higher denaturation temperature in solution and a higher degree of proline hydroxylation, compared with skin collagen of the respective species. The physical properties such as gel strength, melting point and gelling point of conger eel skin gelatin were superior to those of file fish skin and arrow squid skin gelatins.

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Pollutant Loading and Changes of Water Quality at the Namdae-cheon Watershed in Keum river Districts (금강수계 남대천유역의 수질변화와 오염부하량)

  • Choi, Chang-Hyun;Han, Kang-Wan;Cho, Jae-Young;Chun, Jae-Chul;Kim, Seong-Jo
    • Korean Journal of Environmental Agriculture
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    • v.19 no.1
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    • pp.26-31
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    • 2000
  • This study was carried out to investigate the changes of water quality, the pollutant loading by population, livestock, and land use at the Namdae-cheon in Keum river districts. Water samples were taken at the four sites of watershed, the nutrients of which were analyzed periodically from July, 1995 to April, 1998. Content of nutrients in water samples ranged from 1.8 to 5.8 mg/L for total nitrogen, from 0.01 to 0.08 mg/L for total phosphorus and from 0 to 0.007 mg/L for ortho-phosphate respectively. Nitrogen loading by pollutant unit was investigated 550.4 ton/yr as 358.1 ton/yr by livestock, 129.3 ton/yr by land use and 63.0 ton/yr by population. Phosphorus loading by pollutant unit was investigated 79.1 ton/yr as 60.6 ton/yr by livestock, 5.2 ton/yr by land use and 13.3 ton/yr by population. The loading of measured pollutant was investigated 452.5 ton/yr of nitrogen and 5.4 ton/yr of phosphorus in Namdae-cheon watershed.

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A Survey of the Safety in Seasoned Dried Fishes (조미건어포류의 안전성에 대한 조사연구)

  • Yoon, Mi-Hye;Hong, Hae-Geun;Lee, In-Sook;Park, Min-Jung;Yun, Su-Jung;Park, Jeong-Hwa;Kwon, Yeon-Ok
    • Journal of Food Hygiene and Safety
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    • v.24 no.2
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    • pp.143-147
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    • 2009
  • A survey of food preservatives, sulfur dioxide and microbial contamination levels was conducted on 73 seasoned dried fishes(41 squids, 17 filleted fish, 6 Alaska pollack, 9 others) collected from domestic markets and internet. Among preservatives, only sorbic acid was detected in 25 samples including 23 seasoned dried squids, 1 seasoned filleted fish and 1 seasoned dried Alaska pollack with a range $0.01{\sim}0.92g/kg$. Sulfur dioxide was detected from one sample(seasoned dried squid) and its content was 10.5 mg/kg. All samples were contaminated by total aerobic bacteria, and the degree was $3.00{\sim}6.45\;log_{10}$ CFU/g(average level was $5.09\;log_{10}$ CFU/g). Escherichia coli, Salmonella spp. and Staphylococcus aureus were not detected in all samples. On the basis of the results above, most of the seasoned dried fishes were appropriate for Korea food standard, yet biological hygiene control will be needed constantly.