• 대한한방부인과학회지
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• 제30권4호
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• pp.45-58
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• 2017

Objectives: The aim of this trial is to investigate the effect of Ginseng and Wild Cultivated Ginseng to Heat pattern subject. Methods: Eighty-nine Subjects were diagnosed as heat pattern by Cold-Heat Patternization and divided into Ginseng group (n=30), Wild Cultivated Ginseng group (n=31) and Placebo group (n=28) in their 1 st visit. In each visit, The researchers measured the subject's facial temperature using the infrared thermometer (Testo 835-T1). After that, The subjects were asked to mark the current score of flushing on the Visual Analogue Scale (VAS) and to complete the Chalder-Fatigue Scale (CFS) in each visit. The subjects took the test drug for one week and returned the remaining drug on the 2nd visit. The trial result was analyzed with one-way ANOVA using SPSS for Windows version 18. Results: 1. Systolic blood pressure was significantly lower in the Ginseng group and Wild Cultivated Ginseng group than in the control group (p=0.021). 2. There was no significant difference in facial temperature between each groups. 3. The current score of flushing showed the greatest decrease in the Ginseng group compared to the other groups but there was no significant difference (p=0.205). 4. The score of Chalder-Fatigue Scale was decreased in all groups but not statistically significant (p=0.180). Conclusions: This study showed that taking Ginseng extract and Wild Cultivated Ginseng extract do not affect to heat-rising reaction to the subjects diagnosed as heat pattern.

• 대한수의학회지
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• 제25권1호
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• pp.41-48
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• 1985

In the present studies, the effects of dietary high-potassium and high-urea on the metabolism of magnesium, calcium and potassium were checked as an aid to clarify the pathogenesis of hypomagnesaemia(so-called grass tetany) in ruminant. A total of 5 Korean native female goats kept in metabolic cage were received high-potassium(Mg: 0.25%, Ca: 0.94%, K: 5.41%), high-urea(Mg: 0.25%, Ca: 0.94%, K: 0.72%) or control(Mg: 0.25%, Ca: 0.94, K: 0.72%) ration for 15 or 21 days. Daily intakes, fecal and urinary excretions and serum concentrations of magnesium, calcium and potassium were measured with an atomic absorption spectrophotometer. The results obtained were summarized as follows: In high-potassium group, absorption, urinary excretion and serum concentration of magnesium were significantly (p<0.01, p<0.01 and p<0.05 respectively) decreased compared with the control group. On the other hand, the body retention was significantly (p<0.01) increased. However, no clinical symptom of hypomagnesaemic tetany was observable throughout the experimental period. No significant effects on the metabolism and seurm content of calcium were detected in the high-potassium group. In the high-potassium group, significant increase in absorption (p<0.01), urinary excretion (p<0.05) and body retention (p<0.01) of potassium were recognized. However, no significant difference in the concentration of serum was observable between the two groups. In high-urea group, no significant difference in the metabolism of magnesium and potassium or in the serum content were recognized compared with the control group. In high-urea group, tthe absorption, urinary excretion, body retention and serum content of calcium were decreased compared with the control group.

• 대한수의학회지
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• 제33권4호
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• pp.711-717
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• 1993

This study was performed to examine the charges of serum cortisol concentrations on the normal conditions in Korean native goats by enzyme-linked immunosorbent assay(ELISA). The Korean native goats were 4 to 30 months old and weighted 6.0 to 28kg. The goats were allowed ad libitum access to water and food. To minimize the stress with handing and blood sampling, animals were adapted for 2 weeks before the experiment. After adaptation, intravenouse catheter was inserted left intact a jugular vein of 6 goats and blood samples were done 2~3 days later. Experimental animals were divided into 2 groups which was non-catheterized and catheterized group in order to examine the effect for changes of serum concentration and circadian rhythm of cortisol. The results were obtained as follows ; The sensitivity of serum cortisol concentration was $20pg/m{\ell}$. The intra-assay and inter-assay coefficient of variation were below 2.5% and 5%, respectively. Serum concentration of cortisol was more higher in noo-catheterized group$(32.84{\pm}16.78ng/m{\ell})$ than catheterized group$(23.20{\pm}10.29ng/m{\ell})$. The difference according to months old in serum concentration of cortisol was the more higber on 4~6 than 7~12 and lowest over 12 months old. A circadian rhythm in the serum concentration of cortisol in Korean native goats was not found significantly with 2 hours sampling intervals.

• 한국약용작물학회지
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• 제25권6호
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• pp.389-396
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• 2017

Background: Panax ginseng C. A. Meyer is wood-cultivated ginseng (WCG) in Korea which depends on an artificial forest growth method. To produce this type of ginseng, various P. ginseng cultivars can be used. To obtain a WCG similar to wild ginseng (WG), this method is usually performed in a mountain using seeds or seedlings of cultivated ginseng (CG) and WG. Recently, the WCG industry is suffering a problem in that Panax notoginseng (Burk.) F. H. Chen or Panax quinquefolium L. are being sold as WCG Korean market; These morphological similarities have created confusion among customers. Methods and Results: WCG samples were collected from five areas in Korea. After polymerase chain reaction (PCR) amplification using the primer pair labeled with fluorescence dye (FAM, NED, PET, or VIC), fragment analysis were performed. PCR products were separated by capillary electrophoresis with an ABI 3730 DNA analyzer. From the results, WCG cultivated in Korea showed very diverse genetic background. Conclusions: In this study, we tried to develop a method to discriminate between WCG, P. notoginseng or P. quinquefolium using simple sequence repeat (SSR) markers. Furthermore, we analyzed the genetic diversity of WCG collected from five cultivation areas in Korea.

• Korean Journal of Acupuncture
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• 제27권1호
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• pp.31-47
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• 2010

Objectives: To investigate the anti-inflammatory effects of cultivated wild ginseng pharmacopuncture in lipopolysaccharide (LPS)-induced inflammatory rat model. Methods: Sprague-Dawley rats were divided into 4 groups; LPS control (n=6), LPS+cultivated wild ginseng pharmacopuncture at CV4 (n=6), LPS+cultivated wild ginseng pharmacopuncture at CV17 (n=6), and LPS+cultivated wild ginseng pharmacopuncture at Ex-HN1 (n=6). Pharmacopuncture (0.1 ml) was given every two days for 4 weeks followed by inflammation induction by peritoneal LPS injection (5 mg/kg). Blood, liver tissue, and peritoneal lavage fluid were taken and proinflammatory cytokines and other related factors were analysed. Results: Compared with the control group, CV4 and Ex-HN1 pharmacopuncture groups significantly attenuated plasma IL-$1{\beta}$, IL-6, and TNF-$\alpha$ increase at 2h and 5h after LPS injection (P<0.05). A significant difference from control group emerged at 5 h for plasma IL10 (P<0.05). For liver cytokines analyzed at 5 h after LPS injection, only CV4 pharmacopuncture group showed significant difference in TNF-$\alpha$ and IL-10 (P<0.05). Blood CD4/CD8 ratio and the phagocytic activities of polymorphonuclear neutrophils were not different from those of control group in all pharmacopuncture groups (P>0.05). CV4 pharmacopuncture significantly attenuated increase of plasma ${NO_3}^-/{NO_2}^-$, Intracellular adhesion molecule-1 (ICAM-1), cytokine-induced neutrophil chemoattractant-1 (CINC-1), and prostaglandin $E_2$ ($PGE_2$) compared with the control group (P<0.05). Monocyte chemoattractant protein-1, $PGE_2$, and CINC-1 level of CV4 pharmacopuncture group was significantly different from those from the control group (P<0.05). Conclusions: These results indicate that cultivated wild ginseng pharmacopuncture at CV4 may have a potent anti-inflammatory effect in an LPS-induced inflammatory rat model.

• 한국축산식품학회지
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• 제28권4호
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• pp.445-450
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• 2008

The effects of fermented goat milk supplemented with citrus concentrate on blood glucose levels in streptozotocin-nicotinamide-induced diabetic rats were examined. Streptozotocin-nicotinamide-induced diabetic rats (type II) were divided into five experimental groups treated with metformin, goat milk, fermented goat milk, fermented goat milk containing citrus concentrate, or no supplementation (control). The rats in each group were examined weekly for blood levels of glucose, total cholesterol, triglyceride. HDL-cholesterol, LDL-cholesterol. and body weight. On the $24^{th}$ day of the experiment, an oral glucose tolerance test (OGTT) was carried out. Administration of fermented goat milk to the diabetic rats significantly decreased blood glucose and triglyceride levels, while administration of metformin (33.3 mg/kg body weight) did not significantly lower blood glucose levels. Fermented goat milk containing citrus concentrate caused a significant decrease in blood glucose levels in the OGTT at 30 min. This study shows that supplementation with fermented goat milk containing citrus concentrate may be a practical method of reducing blood glucose levels in type II diabetics.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2019년도 학술발표회
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• pp.37-37
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• 2019

기후변화에 따른 강우량 변화는 유역의 수문학적 변화와 하천 침식, 토양유실 등 물리적 변화를 함께 동반하여 유역환경에 영향을 미치게 된다. 최근에도 강우 발생시 집중강우에 의해 짧은 시간에 많은 강우가 발생하여 홍수피해가 발생하고 있으며, 건기시에는 가뭄이 지속되고 있다. RCP 4.5 및 8.5를 활용한 미래 기후시나리오 예측에 따르면 강우량과 증발산량은 증가할 것으로 예측되고 있으나, 우기시의 강수량은 증가하고 건기시의 강수량은 감소하여 계절 간 강수의 불균형이 심화될 것으로 예측되고 있다. 이러한 변화는 강수량, 하천유량, 침투량 등 유역의 수문체계에 영향을 끼치게 되고, 유량의 변화에 따라 하상변동 등 하천형상의 변화가 발생할 수 있으며, 하천 형상변화에 따라 유량이 변화하고, 토사의 유입이 증가하여 수질이 변화하여, 유역 내 유량 및 수질변화에 따른 불확실성이 높아지게 된다. 최근 개발에 의한 불투수면의 증가와 집중호우의 발생으로 인한 유출량 증가에 따라 강우발생시 직접유출량은 증가하고 침투량은 감소하여 우기시에는 홍수 및 침수가 발생하고, 건기시에는 기저유출에 의한 하천유지용수가 감소하여 하천이 건천화되고 수질이 악화 되는 등 물순환 체계가 훼손되고 있다. 강수량과 강우패턴의 변화에 따른 유출량 변화는 유역의 물순환에 직접적인 영향을 미치게 된다. 홍수나 극한강우가 발생할 경우 유역의 수문학적 특성에 따라 직접유출량은 증가하고 상대적으로 침투 및 증발산양은 감소하게 되며, 건기시에는 하천이 건천화 되는 등 물순환 체계가 훼손되고 유량의 변화에 따라 수질 또한 악화될 수 있다. 본 연구에서는 낙동강 유역을 대상으로 기후변화에 따른 유역 유출량 변화 및 하천형상 변화예측을 위해 기후변화 시나리오를 이용하여 하천환경변화에 대한 전망을 예측하고, 유역의 유출량을 구체적으로 분석 할 수 있는 유역모형을 활용하여 유역의 물순환에 미치는 영향을 검토하고 물순환을 고려한 유량 및 수질부하량을 분석하였다.

• 한국잠사곤충학회지
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• 제28권1호
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• pp.37-42
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• 1986

누에의 5령 60시간째 합성효약 홀몬(JHA)을 체중 1g당 1$\mu\textrm{g}$, 10$\mu\textrm{g}$을 경피투여한 후 체액 중 단백질 및 아미노산의 변동을 조사하였던 바 그 결과는 다음과 같다. 1. 5령유충기간은 대조구보다 JHA-1구가 1일, JHA-10구가 4일 연장되었다. 2. 견종 및 견층종은 합성효약 홀몬 투여구가 대조구보다 무거웠고, 견층비율은 JHA-10구가 낮았다. 3. 체액단백질의 농도는 합성효약홀몬투여구가 대조구에 비해 현저하게 증가하였다. 4. 체액 중 전 아미노산양은 JHA-10구가 대조구에 비해 약 2배 정도 많았으며, 특히 glycine와 arginine의 증가 정도는 현저하다.

• 한국콘텐츠학회논문지
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• 제19권4호
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• pp.675-681
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• 2019

이 총설의 목적은 도포 숙성 치즈와 곰팡이 숙성 치즈의 제조 공정의 차이를 살펴보고 이들의 관능적 특성 차이에 관하여 비교 분석 하는 것이다. 도포 숙성 치즈와 곰팡이 숙성 치즈의 관능적 특성은 치즈의 품질 평가에서 가장 중요한 요소이다. 도포 숙성 치즈는 부드럽고 잘 부스러지는 질감을 가지고 있다. 그리고 블루 치즈와 비교하여 짠맛과 신맛이 강한 편이다. 반면에 블루 치즈 (곰팡이 숙성 치즈)는 산양유로 만들었을 때 더 부드럽고 크림과 같은 질감이 나며 냄새가 좀 더 강한 편이다. 블루 치즈는 좀더 산도와 짠맛, 쓴맛이 도포 숙성 치즈에 배하여 강한 편이며 좀 더 신선한 맛이 나는 것이 특징이다. 또한 도포 숙성 치즈와 곰팡이 숙성 치즈의 관능적 특성, 예를 들면 냄새, 맛, 질감 등은 치즈의 품질 평가에 아주 중요한 요소로 작용할 것이라 사료된다.

• 산업식품공학
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• 제21권3호
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• pp.201-207
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• 2017

It is well-known that cultivated wild Panax ginseng has anti-inflammatory effect. However, a comparative study on cultivation period vs biofunctionality is currently lacking. In this study, 70% ethanol extracts of 3-years (yrs)-, 5-yrs-, or 7-yrs-old cultivated wild ginseng were evaluated for their inhibitory effects on RAW264.7 murine macrophages. Specifically, the production of pro-inflammatory cytokines (interleukin-6 [IL-6] and tumor necrosis factor-alpha [TNF-${\alpha}$]), the expression of surface proteins (CD80, CD86, and MHC-II), and the phagocytic properties were investigated. RAW264.7 cells were induced by 500 ng/mL of lipopolysaccharide (LPS) and treated with 0.1, 1, and 10 ppm of samples. LPS-induced IL-6, TNF-${\alpha}$ and surface proteins in all samples were down-regulated in a dose-dependent manner. Both IL-6 and TNF-${\alpha}$ were significantly reduced at 10 ppm of the 7-yrs-old sample compared to 10 ppm of 3-yrs- and 5-yrs-old samples. CD80 and CD86 were also reduced at 10 ppm of all samples, and there was no difference among samples. The phagocytosis has no difference except in 10 ppm of 3 yr-old sample. The results suggest that cultivated wild ginseng extract has anti-inflammatory effect without decreasing phagocytosis.