• Korean Journal of Plant Resources
• /
• v.36 no.1
• /
• pp.26-31
• /
• 2023

In this study, we investigated in vitro inhibitory activity of wild-simulated ginseng (WSG) against non-alcoholic fatty liver disease using HepG-2 cells. T0901317 treatment increased the lipid accumulation in HepG-2 cells, but WSG treatment inhibited T0901317-mediated lipid accumulation. In addition, WSG downregulated T0901317-mediated expression of SREBP-1c, ACC, FAS and SCD-1 protein. In addition, WSG increased the phosphorylation level of LKB1 and AMPK. Compound C treatment blocked WSG-mediated downregulation of SREBP-1c protein. In conclusion, WSG is considered to inhibit the accumulation of lipids and triglycerides in HepG-2 cells by inducing the activation of LKB1 and AMPK successively, thereby reducing the expression of FAS, ACC, and SCD-1 through suppression of SREBP-1c expression.

• Journal of Embryo Transfer
• /
• v.19 no.2
• /
• pp.81-87
• /
• 2004

This study was designed to determine whether repeated superovulation is beneficial for recovery and quality of oocytes in Korean native goats. Seventy-six mature goats, maintained in a pen under natural day length and fed hay ad libitum, were pretreated with progestagen impregnated CIDR for 10 days and then the goats were divided into two groups. One group of the goats received a single intramuscular injection of 1,000 IU PMSG on Day 8 of CIDR insertion. The other group of the goats received twice daily intramuscular injections of a total of 70 mg FSH for 3 days from Day 8 of CIDR. All the gonadotropin treated goats were injected with 10 mg $PGF2{\alpha}on$ Day 8 and 400 IU hCG in the afternoon on Day 10. For oocyte recovery, donor goats were fasted 24 h before operation. Anesthesia was induced by intravenous injection of 2% xylazine(0.2 mg/kg body weight) and ketamin(11 mg/kg body weight). In vivo oocytes were recovered by follicle aspiration or oviduct flushing at 35 to 40 hours after hCG injection through mid-ventral incision. The mean number of CL and oocytes recovered and recovery rate of oocytes by oviduct flushing were greater(P<0.05) in the first treatment than those in the second treatment. Contrary to our assumption, PMSG treatment significantly (P<0.05) increased the number of CL formed and recovery rate of oocytes compared to FSH. However, the same effect was not observed in recovery of follicular oocytes. There was no significant difference in oocyte quality between FSH and PMSG or first and second treatments. The present results indicate that repeated superovulation and repeated use of donor animals may be inefficient for obtaining oocytes in good qualities.

• Journal of Applied Biological Chemistry
• /
• v.61 no.1
• /
• pp.33-38
• /
• 2018

This study aimed to investigate the beauty food activities of wild-cultivated ginseng (Panax ginseng C.A. Meyer). wild-cultivated ginseng extracts were analyzed for antioxidant, skin whitening, anti-wrinkle effect was measured in water and 70% ethanol extract. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical decolorization activities of water and 70% ethanol extracts were 16.69 and 2.18% as well as 4.04 and 3.25% at a solid content of $200{\mu}g/mL$, respectively. The antioxidant protection factors (PF) of water and 70% ethanol extracts at a solid content of $200{\mu}g/mL$ were 1.06 PF and 1.09 PF, respectively. Thiobarbituric acid reactive substance (TBARs) were both 96% at a solid content of $200{\mu}g/mL$. As PF and TBARs showed higher activity than DPPH and ABTS, we could know that antioxidant activity in the lipophilic component of wood-cultivated ginseng were superior to water-soluble component of wood-cultivated ginseng. Tyrosinase inhibitory activity was 10.97 and 52.39% in water and 70% ethanol extracts at a solid content of $200{\mu}g/mL$. The collagenase and elastase inhibitory activities as anti-wrinkle effect were 15.71 and 20.43% in water extracts as well as 32.26 and 86.74% in 70% ethanol extract at a solid content of $200{\mu}g/mL$. The results show that anti-wrinkle effect was the best among the other experiments. This extracts from wood-cultivated ginseng, therefore, seems to be a potent beauty food resource against wrinkles.

• Journal of Embryo Transfer
• /
• v.28 no.1
• /
• pp.19-24
• /
• 2013

This study assesses of efficiency of oocyte recovery and in vitro development for during the non breeding season in goat. Thirty-four matured goats, maintained in a pen under natural day length and fed hay ad libitum, were pretreated with progestagen implanted CIDR for 10 days. Superovulation treatment of the goats received twice daily intramuscular injections of a total of 70 mg FSH for 3 days from Day 8 of CIDR. All the gonadotropin treated goats were injected with 10 mg $PGF_2{\alpha}$ on Day 8 and 400~600 IU hCG in the afternoon on Day 10. Oocytes were recovered by follicle aspiration or oviduct flushing at 35 to 40 h after hCG injection through mid-ventral incision. The in vivo matured oocytes were activated by ionomycin (5 min) and 6-DMAP (3.5~4 h). The activated oocytes were cultured in mSOF medium containing 0.8% BSA at $38.5^{\circ}C$ in an atmosphere of 5% $CO_2$, 5% $O_2$, 90% $N_2$ for 7~8 days. There was no significant difference in the mean number of CL and in vivo matured and follicular oocytes recovered. But, quality of I+II grade follicular oocytes was lower (p<0.05) in the prepubertal goat (25.0%) than the adults (52.4%). The same results were also observed in the cleavage and blastocyst rate of activated oocytes. The clavage and blastocyst rate from prepubertal derived oocytes were lower (p<0.05) in the prepubertal goat (54.5%, 23.3%) than the adult goat (86.8%, 46.6%). Considering overall these results, we suggest that maturation of donor goats is a major factor affecting recovered oocytes quality and in vitro development of activated goat oocytes. There was no significant difference in oocyte quality between seasonal treatments.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.22 no.2
• /
• pp.115-122
• /
• 2002

In this study, the effects of alfalfa hay included in different ratios of 60%(A), 50%(B), 40%(C) and 30%(D) for the diets of young weaned Korean native goats were investigated on feed intake, digestibilities of nutrients, nitrogen retention, average daily gain and feed efficiency. Crude protein contents of experimental diets were 18% in all treatments. The contents of ADF, NDF and crude ash were higher in A as 26.1, 37.0 and 9.0% compared to the lowest values in D of 20.3, 30.4 and 6.6%. However the contents of non-structural carbohydrates was in the opposite tendency. Daily feed intake (DM) was highest in A (414.4g) and B (417.7g) and significantly (p<0.05) lower in D(362.6g). Dry matter intake per metabolic basal weight (DM g/kg of $BW^{0.75}$ ) were highest in A(73.9g) and lowest in D(64.0g). Average daily gain and feed efficiencies were in the range of 33.3~48.7g and 8.1~13.4% respectively with no statistical differences, however with the increasing trends according to higher percent of roughage in the diets. Digestibilities of dry matter (DM), organic matter (OM), crude protein (CP), ADF, NBF, crude ash (CA) and non-structural carbohydrates (NSC) were in the tendency of becoming higher according to higher ratios of concentrate but becoming lower according to higher ratio of alfalfa hay in the diets fed to Korean native goats. Nitrogen retention was lowest in A (1.8g, 15.5%) and highest in D (2.7g, 25.7%). In overall, inclusion of 30~40% alfalfa hay as a roughage in the diets of weaned Korean native goats was superior to the higher rates of inclusion of alfalfa hay in aspects of average daily gain and feed efficiency. The fixed 18% of crude protein content in the diets was efficient for the growth of weaned goats.

• Journal of the Korean Association of Geographic Information Studies
• /
• v.18 no.1
• /
• pp.135-146
• /
• 2015

This study was carried out to understand ecological characteristics of Korean goral such as home range, seasonal home range and habitat use and used for Korean goral restoration and conservation, through a pair of gorals in the north Gyeongbuk province of Korea. With data from GPS collars, we analyzed the behavioral characteristics of the endangered Korean gorals(n=2) in Uljin area from June 2013 to July 2014. As a result, their home range was $1.38{\pm}0.24km^2$ in MCP 95%, $0.81{\pm}0.09km^2$ in FK 95% and $0.15{\pm}0.16km^2$ in FK 50% (t=8.118, p>0.05). Seasonal home range for MCP 95% was $0.74{\pm}0.31km^2$ > (winter) > $0.71{\pm}0.27km^2$ (spring) > $0.61{\pm}0.06km^2$ (autumn) > $0.27{\pm}0.04km^2$ (summer) (F=2.135 p>0.05). It showed that home range in winter was the largest and that in summer was the smallest. Mean elevation of male for seasonal habitat use was $440.18{\pm}71.32m$ in summer and that of female was $727.25{\pm}99.98m$ in spring. The lowest altitude for male was $372.72{\pm}70.79$ and female was $664.60{\pm}139.71m$. It meant that there were seasonal change and thus annual and seasonal behavior characteristics for both sexes had different correlation according to elevation change. Although in this study the correlation with prey in habitat and the changes of habitat disturbance were not clearly investigated, We could understand goral home range and habitat use through research data.

• Journal of Applied Biological Chemistry
• /
• v.65 no.4
• /
• pp.253-259
• /
• 2022

The aim of this study was to investigate the correlation between growth characteristics and ginsenoside contents of 4-year-old wild-simulated ginseng cultivated in different regions. Most of the soil properties except for available phosphate showed significantly higher in Pyeongchang than in other cultivation sites. The growth characteristics except for root length and number of rootlets showed significantly higher in Pyeongchang than in other cultivation sites. In the case of 8 ginsenoside contents, the content of F2-AS was significantly higher in Muju than in other cultivation sites and the content of F1 in Yeongju was significantly high. In Yeongwol, the contents of Rb1 and Re-p were significantly high and the content of Ro in Pyeongchang showed significantly higher than in other cultivation sites. Root length and soil pH did not show a significant correlation with any soil properties and growth characteristics of wild-simulated ginseng, respectively. Most of the growth characteristics showed significantly positive correlations with electrical conductivity, organic matter content, total nitrogen content, exchangeable cations (K⁺, Ca²⁺, Mg²⁺), and cation exchange capacity. Rb1 and Re-p showed significantly negative correlations with most of the growth characteristics of wild-simulated ginseng except for the number of rootlets. Ro showed a significantly positive correlation with stem length, number of leaflets per stem, leaflet length, leaflet width, and root diameter. The results of this study probably will help to provide useful information on the establish a quality standard by investigate correlation analysis between growth characteristics and ginsenoside content of 4-year-old wild-simulated ginseng.

• Journal of Animal Science and Technology
• /
• v.45 no.6
• /
• pp.1019-1030
• /
• 2003

The study was conducted to isolate and identify highly fibrolytic anaerobic fungi from the guts of a Hanwoo steer and a Korean native goat, and then investigate the characterization of cellulolytic activity of an anaerobic fungus. Twenty-one anaerobic fungal colonies were isolated in the study, in which 16 colonies were isolated from the rumen contents of the Hanwoo steer and 5 colonies from the duodenal fluids of the Korean native goat. Four anaerobic fungi were selected based on higher cellulolytic enzyme activities to identify under a optical microscope. NLRI-M003 and -T004 belong to Neocallimastix genus and NLRI-M014 belongs to Piromyces genus based on the morphology of their thallus, sporangia, rhizoid and the number of flagella. NLRI-M001 appeared to be an unknown strain of anaerobic fungi due to its different morphology from existing types of anaerobic fungi, though the morpholgoy is similar to Orpinomyces sp. Supplementation of 2% anaerobic fungal culture(NLRI-M003) in rumen-mixed microorganisms increased in vitro DM degradability of rice straw and filter paper up to 4 and 11%, respectively, compared with non-supplementation(control). CMCase and xylanase activities in in vitro culture were also higher in 2% fungal supplementation than controls in both rice straw and filter paper substrates.

• Journal of Embryo Transfer
• /
• v.17 no.3
• /
• pp.171-177
• /
• 2002

Glucocorticoid is activating mammary gland cells for lactating animals, resulting in increasing abilities of the milk synthesis. Expression of the prolactin receptor(PRL-R) in mammary gland cells was closely associated with milk production. To increase lactation ability for the Korean Native Goats at mid-lactation period. 0.05, 0.1. and 0.2 g of hydrocortisone was administrated with 5 $m\ell$ of saline. and injected into vein. For the control, 5 $m\ell$ of saline was administrated in to vein. After 24 H, the mammary gland tissue was collected, and mRNA expression rates were investigated for the alpha-casein and PRL-R using competitive PCR(polymerase chain reaction). There was no significant differences between treatment and control groups for the mRNA expression rate of PRL-R in mammary gland cells after 24 h of administration of hydrocortisone. The rate of mRNA expression for the alpha-casein was increased 37%, 630%, and 380% at 0.05, 0.1, and 0.2 g of hydrocortisone administration groups, respectively, comparing with control group. The results suggested that PR L-R mRNA expression of mammary gland cell by administration of hydrocortison was not significant, but increase of the alpha-casein mRNA expression my be differences of expression of functional proteins in the cell and expression patterns of protein secretion time to out of the cell. This study showed increase of alpha-casein mRNA expression by administration of hydrocortisone at mid-lactation period of Korean native goat.

• Korean Journal of Animal Reproduction
• /
• v.22 no.1
• /
• pp.29-34
• /
• 1998

Jugular plasma concentrations of luteinizing hormone, prolactin, estradiol-17\ulcorner and 13, 14-dihydro-15-keto-prostaglandin-F2봬(PGFM) were meausred prepartum during the last 12 days of pregnancy, at parturition, then 1 day after parturition in 16 goats. Plasma samples were analyzed for luteinizing hormone(LH), estradiol-17\ulcorner(E2), prolactin(PRL) and prostagladin F2봬(PGF2봬) concentrations by radioimmunoassay. 1. The concentrations of plasma luteinizing hormone in Korean native goats remained fairly constant(0.20 0.02\ulcorner0.38 0.04 mlu/ml) from 12 days prepartum to 1 postpartum but the concentrations of plasma prolactin rose slightly from 1 day prepartum. 2. The estradiol-17\ulcorner concentrations increased rapidly after day 1 before partum, reaching a peak at parturition(74.8 77.5 pg/ml), and falling to 63.8 2.8 pg/ml at day 1 postpartum. 3. Starting at 323.2 69.6 twelve days before parturition, the concentrations of plasma prostaglandin F2봬 rose during the 1 day preceeding parturition(650.7봬57.8 pg/ml) and peaked at 1081.4 164.9 on the day of parturition. At day 1 postpartum, the concentrations of PGF2봬 decreased to 425.3 60.4 pg/ml. Finally, these results show that changes in prostaglandin F2봬 concentrations before parturition were closely related to changes in estradiol-17\ulcorner concnetrations, but after parturition they remained elevated whereas estradiol-17\ulcorner concentrations fell abruptly.