• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.66-66
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• 2018

국화과(Compositae) 다년생 초본인 산국속(Dendranthema)은 국내 약 13여종이 자생하는 것으로 알려져 있으며, 이 중 감국(D. indicum (L.) Des Moul.)과 산국(D. boreale (Makino) Ling ex Kitam.), 구절초(D. zawadskii var. latilobum (Maxim.) Kitam.)가 주로 차 또는 한약재 등의 원료로 이용되고 있다. 차로 이용되는 꽃은 산국이 감국에 비해 상대적으로 작아서 구분이 가능하지만 시중에는 건조된 형태로 가공 유통되므로 육안으로 구분이 쉽지 않고, 산국 유래 제품들은 국내에서 감국 또는 국화로 혼용해서 표기되어 유통되고 있어 그 기원을 명확히 정립할 필요가 있다. 이에 본 연구는 감국과 산국의 분자유전학적 판별을 위해 DNA 바코드 후보 유전자를 활용하여 염기서열분석으로 확보된 SNP 및 InDel 정보를 바탕으로 CAPS 마커를 개발하고자 수행되었다. 감국과 산국 모두 trnL-trnF intergenic spacer 구간에서 약 1kb의 PCR 산물이 확인되었고, 이들 염기서열에서 분석한 2 SNP 및 3 InDel을 대상으로 CAPS 마커 개발을 위한 제한효소 사이트를 탐색하였다. Gap을 포함한 774bp (감국/산국=A/G) 위치의 SNP에서 BstUI(GC^GC)처리로 CAPS 마커로 전환 가능함이 확인되었고, 이에 감국과 산국의 PCR 산물에 제한효소를 처리한 결과, 제한효소 인식 사이트가 존재하는 산국에서 두 개의 DNA 단편이 확인되었다. 위 결과는 다양한 형태로 가공 유통되는 감국과 산국의 판별을 위한 마커로 활용될 수 있으며, 본 연구에 활용된 기술은 추후 건강기능식품 개발을 위한 원료표준화 확립 연구에 유용할 것으로 판단된다.

• Food Science and Preservation
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• v.5 no.3
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• pp.299-304
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• 1998

Screening for antibacterial activities with microorganisms related to the food putrefaction by ethanol extract from Chrysanthemum petals widely used for the traditional wine production, and antibacterial activities of each concentration and minimum inhibitory concentration(MIC) from the ethanol extract were researched. Antibacterial activity of ehanol extract for B. subtilis was higher in C. boreale than in C. Morifolium, but that of E. coli was higher in C. molifolium than in C. boreale. C. boreale was higher than C. morifolium in the antibacterial activity of ehanol extract and MIC of ehanol extract from C. boreale was 60-70${\mu}\ell$/ml. Ethanol extract from C. boreale was higher Gram(-) than Gram(+) in the antibacterial activities, but Gram(-), Gram(+) were greatly inhibited on growth in 100${\mu}\ell$ concentration.

• Applied Biological Chemistry
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• v.42 no.2
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• pp.176-179
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• 1999

In the bioactivity test of nine sesquiterpene lactons isolated from Hemisteptia lyrata, Chrysanthemum zawadskii and Chrysanthemum boreale, we investigated their antimicrobial activities against ten bacteria and six phytopathogenic fungi. Hemistepsin A and hemistepsin B isolated from H. lyrata and tulipinolide isolated from C. boreale exhibited strong antibacterial activity. In the antifungal test, hemistepsin B, angeloylcumambrin B, tigloylcumambrin B and costunolide showed broad activity. Both antibacterial and antifungal experiments exhibited quite clear specificity against microbial strains.

• Applied Biological Chemistry
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• v.45 no.2
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• pp.108-113
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• 2002

The compositions of essential oils isolated from the aerial parts of Chrysanthemum boreale and C. indicum by steam distillation were analyzed by GC and GC-MS. Ninty-four components were identified in the essential oil from C. boreale, with camphor (15.40% as GC peak area), cir-chrysanthenol (14.11%), ${\alpha}-thujone$ (13.27%), 1,8-cineole (4.16%), ${\alpha}-pinene$ (3.80%), ${\beta}-caryophyllene$ (3.58%), gremacrene D (2.69%), camphene (2.40%), umbellulone (2.24%) and ${\beta}-pinene$ (2.00%) as the major constituents. In the oil from C. indicum, the major constituents among 80 components identified were germacrene D (16.50%), camphor (10.04%), ${\alpha}-thujone$ (6.40%), ${\alpha}-pinene$ (4.50%), ${\alpha}-cadinol$ (3.97%), camphene (3.82%), ${\beta}-pinene$ (3.67%), zingiberene (3.64%), cis-chrysanthenol (3.45%), piperitone (3.09%), 1,8-cineole (2.61%) and chrysanthenone (2.42%). The oil from C. boreale, although similar to that from C. indicum in many respects, contained proportionately higher levels of camphor, cis-chrysanthenol, ${\alpha}-thujone$, 1,8-cineole and umbellulone, while that from C. indicum contained higher levels of germacrene D, ${\beta}-caryophyllene$, ${\alpha}-cadinol$, zingiberene, cis-chrysanthenol and piperitone.

• Journal of Life Science
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• v.29 no.4
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• pp.442-446
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• 2019

We recently reported the characteristics of Chrysanthemum boreale MAKINO essential oil (CBMEO) at different harvesting stages and its in vitro antioxidant, whitening, skin regeneration, and antiwrinkle properties. In the present study, we investigated the effectiveness of cosmetic creams containing 0.1% of CBMEO in terms of wrinkle reduction and safety. The participants were 22 women aged 42 to 60 y with natural wrinkles. All the participants applied the CBMEO-containing cream to the eye area for 8 weeks. Visual observations of the skin by a dermatologist were used to assess the safety of the product, and improvements in skin wrinkles (roughness) were analyzed using a skin visiometer. Neither the CBMEO-containing cream nor the placebo caused abnormal reactions, such as erythema or allergies, during the test period. The CBMEO-containing cream-exposed test regions showed a greater reduction in the roughness index as compared with those of the placebo-exposed control regions. The R1, R2, and R3 indices showed a statistically significant decrease in the test regions as compared with the placebo control regions after using the cream for 2 weeks (p<0.05). Therefore, this study demonstrated that CBMEO-containing cosmetics are safe for human use and that they have skin wrinkle improvement properties. In the future, CBMEO may be a promising product for use in the development of functional anti-wrinkle cosmetics.

• Korean Journal of Medicinal Crop Science
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• v.11 no.1
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• pp.1-4
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• 2003

Two antioxidative compounds in Chrysanthemum boreale were isolated by a bioassay using a 2, 2-diphenyl-1-picrydrazyl(DPPH) free radical. They were identified as apigenin, linarin(acacetin-7-O-rutinoside) on the basis of $^1H\;and\;^{13}C-NMR$ and MS data. The DPPH radical scavenging activity$(RC_{50}:\;13.3{\mu}g)$ of apigenin was similar to that of ${\alpha}-tocopherol\;(12.1{\mu}g)$ and L-ascorbic acid$(13.1{\mu}g)$

• Applied Biological Chemistry
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• v.38 no.3
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• pp.269-272
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• 1995

Antibacterial activity test of solvent fractions, sesquiterpenoid lactones, and Compound I and II extracted from Chrysanthemum boreale M. and Chrysanthemum indium L. were performed against four microorganisms. Among the tested substances, antibacterial activities were appeared against B. subtilis and V. parahaemolyticus at the chloroform fraction, sesquiterpenoid lactones and Compound I extracted from C. boreale. But chloroform fraction and sesquiterpenoid lactones extracted from C. indicum were showed weakly than those of C. boreale. Compound II and all fractions extracted from C. indicum were not appeared against the all tested microorganisms.

• Applied Biological Chemistry
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• v.39 no.4
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• pp.315-319
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• 1996

The yields of methanol extracts of several Chrysanthemum spp.(5 species, 9 parts) and their solvent fractions were investigated. The yields of methanol extracts ranged from 16.9%(for flower of C. indium, Cultivated) to 31.5%(for whole plant of C. indium). In the tests of the antibacterial activity. the methanol extracts from flower of C. zawadskii and C. boreale, whole plant of C. zawadskii, and flower of C. coronarium showed excellent antibacterial activity. Generally, the chloroform fractions exhibited stronger antibacterial activity against all the bacteria tested when compared with other solvent fractions. In case of C. boreale, the chloroform fraction of the whole plant was shown stronger antibacterial activity against all four bacterial strains tested when compared with that of the flower. The chloroform fractions from flower and whole plant of C. boreale and C .zawadskii, and flower of C. coronarium were shown a similar TLC pattern.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.7
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• pp.824-831
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• 2009

In order to investigate the quality characteristics of powdered teas using Compositae species flower, the samples of Chrysanthemum indicum L. (CI), Chrysanthemum boreale Makino (CB), and Chrysanthemum zawadskii var. latilobum (Maxim.) Kitam (CZ) were extracted with hot water and concentrated, and then mixed with different forming agents of dextrin (D) and maltodextrin (MD). The mixing ratio of D and MD was ranged from 9:1 (DMD91) to 8:2 (DMD82) or D and MD only. The solubility of the powdered tea was higher in added dextrin. The highest total polyphenol and flavonoid content of the powdered tea were 6.75 and 3.24 mg/g at CBDMD91, respectively. Total antioxidant activities of C. indicum, C. boreale, and C. zawadskii powdered tea ranged at $2.51{\sim}2.63$, $4.37{\sim}4.50$, and $3.44{\sim}3.55\;mg$ AA eq/g, respectively. In sensory evaluation, the C. indicum and C. zawadskii powdered teas obtained higher sensory score of all evaluation items than C. boreale powdered tea. The optimal mixing ratio at 36% of D and 4% of MD in forming agent was selected to enhance the sensory characteristics and antioxidant activities of powdered tea.

• Korean Journal of Plant Taxonomy
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• v.39 no.1
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• pp.29-34
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• 2009

The Chrysanthemum zawadskii complex is demarcated from other species by having the white and pink ligulate flowers. Its morphological characters are greatly diversified, so that various classification systems have been suggested. The character of leaf thickness has been mentioned as the characteristic for recognizing some of infra-specific taxa within this complex. In this study, we used longitudinal leaf sections to investigate the leaf thickness and cell number of leaf blades of 13 populations including those of the members of the C. zawadskii complex, as well as 4 populations of the related species of C. boreale and C. indicum. From the result, it was clear that the leaves were thicker in populations of C. boreale, C. indicum and C. zawadskii complex (diminishing in that order), and that the leaves were composed of about 9 cell layers in all populations. Within the C. zawadskii complex, leaf shape and thickness varied among the populations. It was very interesting that the taxa with restricted distribution, like C. zawadskii var. tenuisectum, C. zawadskii var. alpinum, C. zawadskii var. lucidum, and C. zawadskii subsp. coreanum had a thicker leaves than found among widely occurring taxa. From this, leaf thickness is supposed to be an adaptation to the unique habitat of each population.