• Korean Journal of Plant Resources
• /
• v.25 no.2
• /
• pp.240-245
• /
• 2012

In this study, we investigated the boosting effects on collagen biosynthesis of $Anthriscus$ $sylvestris$ ethanol extract (ASE) in human dermal fibroblasts. To obtain more effective fraction and subfraction for collagen biosynthesis, standard solvent partition and open column chromatography were performed. The EtOH extract, solvent fractions, and 8 EtOAc subfractions were tested for their collagen synthesis capacity by [$^3H$]Proline-incorporation and ELISA assay. ASE increased 25% of total collagen synthesis and 27% of procollagen biosynthesis. The total collagen biosynthesis was increased by EtOAc fraction and E6 subfraction to 28% and 50% respectively. Type I procollagens were also upregulated by EtOAc fraction and E6 subfraction to 30% and 47%, each. Taken together, our data suggest that potential anti-aging effect of ASE on skin is via increasing collagen biosynthesis and effective subtraction is E6 subfraction of EtOAc fraction.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.31 no.2
• /
• pp.252-258
• /
• 1998

A feeding experiment was conducted to investigate the effects of deletion of P, Ca, Zn, Mg, Fe, K, Mn, or Se from mineral premix in the diets containing $40\%$ fish meal on growth performance of juvenile Korean rockfish. Three replicate groups of the fish initially averaging 5.4 g were fed the experimental diets deleted one ot each mineral (P, Ca, Zn, Mg, Fe, K, Mn, Se or all) in the mineral premix for 9 weeks. A basal diet with fish meal ($40\%$), meat meal ($9\%$), soybean meal ($5\%$), corngluten meal ($3\%$), and leather meal ($3\%$) as protein sources were included. Weight gain in fish fed diets deleted Ca, Zn, K, or Se was lower than that of control diet ( P<0.05). Daily feed intake, condition factor, hepatosomatic index and liver chemical composition were not affected by dietary mineral premixes (P>0.05). Apparent skeletal deformities were not observed in the diets deleted each mineral, The data obtained in this study indicate that each of the Ca, Zn, K, or Se should be added in the diet contained $40\%$ fish meal for normal growth of Korean rockfish.

• Microbiology and Biotechnology Letters
• /
• v.26 no.3
• /
• pp.200-205
• /
• 1998

A probiotic-strain of Lactobacillus sp. was cultured in bovine blood plasma-based (BBPB) medium and freeze-dried to prepare a probiotic product as an animal feed additive. The cell mass produced in the medium, $5.2{\times}10^9$ CFU/ml, was high enough to be commercialized and was 74% of that in MRS medium. The survival rate of tactobacillus sp. against freeze-drying was affected by the conditions for treatment of cultured BBPB broth before freeze-drying such as pH adjustment, volume reduction and freezing rate. It was also found that the blood protein hydrolysate remaining in broth also enhanced the survival rate. Among various protective substances, sucrose showed a high stabilizing effect with 10% (w/v) addition, by which the maximum survival rate (48.3%) and viable cell count ($3.0{\times}10^{10}$ CFU/g) were obtained.

• Journal of Life Science
• /
• v.12 no.5
• /
• pp.523-527
• /
• 2002

This study was carried out to investigate that physiologiral activity was relevence to mocroorganism and contents of sinigrin in Dolsan Leaf Mustard Kimchi (DLMK) during fermentation. DLMK was prepared from Dolsan leaf mustard, green onion, garlic, red pepper powder, ginger and salt. And it was fermented at 2$0^{\circ}C$ for 50days. The number of total microbes were increased until reaching the optimum ripening period and after, that number slowly decreased. And that tendency was agreement with antioxidative activity, And also the contents of sinigrin was increased until the optimally ripened time, then decreased. These results suggests that microorganism was significantly related to the physiological activity, and sinigrin was the one of the physiological active substances by microorganism in DLMK. In particular, 50days fermented DLMK at 2$0^{\circ}C$ was showed the highest contents of crude protein. Coincidently, 50days fermented DLMK might possibly contain high levels of crude proteins produced by various microorganism.

• Journal of the Korean Recycled Construction Resources Institute
• /
• v.4 no.4
• /
• pp.111-116
• /
• 2009

In order to verify relevance propriety as material for improving and replacing agricultural land of soil(the rest is sorting soil) produced in treatment process of construction waste, this study executed physical, mechanics and soil analysis test with mixing sorting soil and farm land, crops rearing comparison test with replacing lower layer soil.

• Journal of Animal Environmental Science
• /
• v.9 no.1
• /
• pp.35-44
• /
• 2003

This study was conducted to determine effects of and a proper level of barley malt sprouts(BMS) addition as well as to develop an effective fermentation method when broiler litter(BL) was ensiled or deepstacked with 0 to 10% levels of BMS. Mixtures were ensiled or deepstacked for one month and physico-chemical analyses were made between before and after treatments. Addition of BMS up to 10% enhanced nutritional quality of either of ensiled or deepstacked BL mixtures. Especially, the quantity and quality of protein were improved with the addition of BMS to BL. However, the still high pH values after ensiling of various mixtures indicated that anaerobic fermentation did not effectively occur with the addition of up to 10% of BMS to BL and consideration of fermentation aids appeared to be necessary. For deepstacking, addition of BMS to BL increased internal peak temperature(from 56 to $70^{\circ}C$) of the stack, indicating that the added BMS to BL stimulated the degradative activity of fermentative microorganisms. The deepstacking method was more effective than the ensiling method for the manufacture of hygienical fred mixture with BL and BMS.

• Korean Journal of Food Science and Technology
• /
• v.34 no.5
• /
• pp.762-769
• /
• 2002

As a preliminary test for defining intact yellow croaker pigment, the pigment was analyzed by column chromatography and UV-vis spectrophotometry. All maximum absorbance wavelengths commonly showed three maximum absorbance ranges, similar to those of carotenoid, suggesting that the tested pigment may be carotenoid. We detected total six peak RT values in the chromatogram through PDA-HPLC under gradient mode (behavior A at 10% for initial 2 min and changed to behavior B for 60 min). Most pigments were detected at the peak with 3.27 RT value. Because seven peaks were detected under gradient mode and three under isocratic mode [methanol : methylene chloride (90 : 10, v/v)], gradient mode was determined to be more appropriate for quantitative analysis. By the comparison test of RT values among yellow pigment in croakers and reference pigments, such as zeaxanthine, ${\beta}-cryptoxanthine$, ${\beta}-carotene$, and astaxanthin, only ${\beta}-cryptoxanthine$ was detected in the white croaker, whereas such pigment of yellow croaker having RT value of 31.02 was not detected. Therefore, RT value was found to be applicable for detecting adulterated croaker.

• Journal of Aquaculture
• /
• v.7 no.2
• /
• pp.97-107
• /
• 1994

Experimental diets containing 5 different levels of a-cellulose $(0\%,\;5\%,\;10\%,\;15\%\;or\;20\%)$ were fed to juvenile Korean rockfish (20.5g of initial mean weight) for 10 weeks to study their ability to utilize dietary $\alpha-cellulose$. Weight gain, feed efficiency, and protein and energy retention efficiency in the fish fed diets containing $0\%,\;5\%,\;or\;10\%$ of $\alpha-cellulose$ were significantly higher than those in the fish fed diets containing $15\%\;or\;20\%$ of $\alpha-cellulose$ (P<0.05). Whole body lipid content of the fish fed $10\%$ $\alpha-cellulose$ diet were significantly higher than that of fish fed other diets (P<0.05). The results suggest that the optimum dietary fiber levels for Korean rockfish would be less than $10\%$.

• Journal of Aquaculture
• /
• v.5 no.1
• /
• pp.9-17
• /
• 1992

To test the availability of soybean meal as a protein source and to increase its effectiveness in the diets for the fingerlings of red seabream, some feeding trials were conducted. Two different types of diet, steam pellet and soft dry pellet, contained various levels of soybean meal, were used. The growth, feed efficiencies, and body compositions were examined after the feeding trials. Results of the experiments suggested that dietary protein level could be reduced from $52{\%}$ to $47{\%}$ by elevating dietary energy level from $13{\%}$ to $21{\%}$ with lipid. The best results of growth and feed efficiencies were achieved by the diet that containing $47{\%}$ of crude protein and $20{\%}$ of crude lipids. The growths and feed efficiencies showed no difference when $20{\%}$ of fish meal in the experimental diet were substituted by soybean meal. Therefore, $20{\%}$ of fish meal could be substituted by soybean meal without reducing growth rates or feed efficiencies of fingerling red sea bream.

• Journal of Animal Environmental Science
• /
• v.11 no.1
• /
• pp.61-70
• /
• 2005

A total of 4 trials were conducted to evaluate the nutritional value of food waste-byproduct feeds mixture(FWM) rotary drum-pasteurized and cured, to trace the nutritional flow according to the processing stages and finally to determine the optimal mixing formulation of feed ingredients for the growing pigs. Pasteurizing and curing FWM using a rotary drum drier resulted in little change in chemical composition and 8 to $15 \%$ of dry matter losses. Chemical change was affected by different feed ingredients used in each trial. Curding of FWM was caused probably by high moisture content, starch content and(or) physical characteristics of feed ingredients. The optimal mixing ratio of feed ingredients was food waste 45 : deepstacked broiler litter 10 : bakery by-product 22.5 barley bran 22.5. In conclusion, FWM rotary drum-pasteurized and cured had a satisfactory feed-nutritional value far growing pigs.