• Title/Summary/Keyword: 비환원주의

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Effect of Bean Water Concentration and Incubation Time on Amylase Activity and Physicochemical Characteristics of Yukwa Paste (콩물 농도 및 숙성 시간이 Amylase 의 활성과 유과 반죽의 특성에 미치는 영향)

  • Sohn, Kyung-Hee;Jo, Mi-Na;Jeon, Hyeong-Ju;Park, Jin;Joo, Myung-Sook
    • Korean Journal of Food Science and Technology
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    • v.33 no.3
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    • pp.288-293
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    • 2001
  • ${\alpha}-amylase$ activities of bean water was not significantly influenced by bean water concentrations but they were remarkably influenced by different temperatures and substrates. ${\alpha}-amylase$ activities of bean water on cooked starch were significantly higher than those on raw starch. ${\beta}-amylase$ and glucoamylase activities in 14% bean water were significantly higher than those in 7% bean water. Yukwa paste is glutinous rice flour paste. Bean water was added to Yukwa paste by 0, 7, 14% and incubated 0, 3, 6, 9, 12 hours at $60^{\circ}C$. The pH of Yukwa paste increased with bean water concentration and decreased with the incubation time. The viscosity decreased with bean water concentration and incubation time. The ruducing sugar content of Yukwa paste increased with bean water concentration and incubation time. The changes of reducing sugar content in cooked Yukwa paste were much higher than those in the raw one. ${\alpha},\;{\beta}-amylase$glucoamylase activities of Yukwa paste also increased with bean water concentration, and their activities were much higher on the cooked glutinous rice flour than those on the raw one. The SEM observation on the freeze-dried flour of Yukwa paste showed breakdown of amylopectin structure by addition of bean water in the paste.

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Characteristics of the Stored Samjangs with Different Doenjangs (원료된장을 달리하여 제조한 저장쌈장의 품질특성)

  • Kim, Hye-Lim;Lee, Taik-Soo;Noh, Bong-Soo;Park, Jung-Suk
    • Korean Journal of Food Science and Technology
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    • v.31 no.1
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    • pp.36-44
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    • 1999
  • Samjangs which were prepared using magjang, traditional doenjang, and mixture of traditional doenjang and magjang were stored. Characteristics of the stored samjangs were investigated. Maximum titratable acidity was shown in traditional doenjang after 30 day of storage. Total sugar in samjangs decreased while reducing sugar increased to $11.45{\sim}12.35%$ from $8.44{\sim}9.29%$ after 40 days. Amino type nitrogen predominantly increased to $337.3{\sim}381.1\;mg%$ after 40 days from $48{\sim}53\;mg%$ of initial period of preparation. Among the free amino acids the content of glutamic acid $(202.6{\sim}464.6\;mg/100g)$ was highest and others were not too much changed. Forty two components including 7 alcohols, 4 esters, 13 acids, 6 aldehydes, 5 phenols, 3 pyrazines and others were found in samjangs. Ethanol, acetic acid ethyl ester and 2-phenylethanol were found in all treatments and ethanol, acetic acid ethyl ester, phenylacetaldehyde, butanoic acid, acetic acid, 3-methyl butanoic acid and 2,4-hexadienoic acid might be major volatile components considering of high peak area. 2-Phenylethanol, butandioic acid ethyl ester, butanoic acid and 2-methyl-2-butenoic acid were higher than other components in samjang with magjang while ethanol, acetic acid, 2,4-hexadienoic acid were in samjang with traditional doenjang and 3-methyl butanoic acid, acetic acid ethyl ester and methyl pentanoic acid were in the mixed treatment.

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Biosynthesis of Polyhydroxyalkanoates and 5-Aminolevulinic Acid by Rhodopseudomonas sp. KCTC1437 (Rhodopseudomonas sp. KCTC1437에서의 Polyhydroxyalkanoates와 5-Aminolevulinic Acid의 생합성)

  • 이영하;기형석;최강국;문명님;양영기
    • Korean Journal of Microbiology
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    • v.38 no.2
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    • pp.144-151
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    • 2002
  • For elucidating the relationship between the biosynthetic pathways for polyhydroxyslkanoates (PHAs) and 5-aminolevulinic acid (ALA), culture conditions for the production of these two biomaterials by Rhodopseudomonas sp. KCTC 1437 were investigated. Of the carbon substrates tested, acetic acid was the best carbon source for cell growth and PHA biosynthesis. When succinic acid was added as a co-substrate into culture medium, cell growth and PHA production were greatly increased up to 2.5 g/ι and 73% of dry cell weight, respectively. The PHA obtained from the carbon substrates tested was homopolyester of 3-hydroxybutyrate, while valeric acid was only effective for the production of copolyester consisting of 3-hydroxybutyrate and 3-hydroxyvalerate. Anaerobic light culture condition was better for PHA production and cell growth than anaerobic dark or aerobic dark culture condition. The organism was capable of synthesizing ALA when glycine and succinic acid were added to the culture medium. ALA was produced to ca.400 mg/ι when levulinic acid, soccinic acid, and glycine were repeatedly added with a reductant (sodim thioglycolate). However, the presence of glycine, levulinic acid and sodium glycolate inhibited the cell growth and the conversion of carbon substrates to PHA. From these results it is apparent that the production yields of PHA and ALA could not be increased simultaneously because the optimal conditions for the production of PHA and ALA are opposed to each other.

Changes in Chemical and Sensory Properties of Dongchimi during Fermentation (동치미의 발효 중 화학적 및 관능적 성질의 변화)

  • Kang, Kun-Og;Sohn, Hyun-Joo;Kim, Woo-Jung
    • Korean Journal of Food Science and Technology
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    • v.23 no.3
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    • pp.267-271
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    • 1991
  • The chemical and organoleptic properties of dongchimi studied for their changes during fermentation at $4{\sim}35^{\circ}C$ in 7% NaCl solution with seasonings. It was found that pH decrease showed three variation points in its slope at about pH 5.2, 4.7 and 4.1 during fermentation. A negative linear relationship between total acidity and logarithmic value of pH was obtained. The slope of the relationship curve was increased as the fermentation temperature increased. The reducing sugar was increased until pH reached$4.0{\sim}4.2$ followed by a small decrease and its amount was lowered as the temperature increased from $4\;to\;25^{\circ}C$ The major nonvolatile organic acids were lactic and citric acids. The increase in lactic acid were more marked at $25^{\circ}C$ than at $4^{\circ}C$. As fermentation proceeded the fresh radish odor significantly decreased while sourness and yeast-moldy odor increased. The crispness of the radish decreased slightly during fermentation.

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Improvement of Electrochemical Reduction Characteristics of Carbon Dioxide at Porous Copper Electrode using Graphene (그래핀을 이용한 다공성 구리 전극의 전기화학적 이산화탄소 환원 능력 향상)

  • Bang, Seung Wan;Rho, Hokyun;Bae, Hyojung;Kang, Sung-Ju;Ha, Jun-Seok
    • Journal of the Microelectronics and Packaging Society
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    • v.25 no.4
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    • pp.105-109
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    • 2018
  • We studied graphene synthesis to porous Cu to improve the characteristics of carbon dioxide reduction of cu. Cu powders were formed through Thermal Chemical Vapor Deposition(TCVD) to Porous Cu/Graphene structures synthesized with graphene. As a result of electrochemical experiments using a 0.1 M $KHCO_3$ electrolyte at an applied potential of -1.0 V to -1.4 V, the current density of Porous Cu/Graphene was 1.8 times higher than that of Porous Cu. As a result of evaluating the product, CO and $H_2$ were generated to Porous Cu electrode. On the other hand, the product of porous Cu/Graphene produced CO, $CH_4$ and $C_2H_4$. It is considered that the graphene causes longer carbon dioxide adsorption time, which means that the intermediates formed during the reaction remain on the electrode surface for a longer time. As a result, it can be concluded that the production reaction of the C2 compound could be continuously performed.

Study on Quality Characteristics of Korean Traditional Kukhwaju by Addition of Dried Chrysanthemum indicum L. Extract into Mash (건조된 감국 열수추출물의 첨가 방법에 따른 국화주의 특성 연구)

  • Bang, Byung-Ho;Paik, Jean Kyung;Choi, Jea Young;Jeong, Eun-Ja;Rhee, Moon-Soo;Yi, Dong-Heui
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.9
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    • pp.1333-1338
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    • 2015
  • This study investigated the effects of addition of Chrysanthemum indicum L. extracts on quality characteristics of Kukhwju during fermentation. Fermented liquors without C. indicum L. were used as the control group. For experimental groups, we added 1, 2, 5, and 10% C. indicum L. extracts and brewed according to the addition method written in Yorok. The pH level during fermentation phase, alcohol concentration, level of reducing sugars, and acidity were measured in each of the experimental groups. After fermentation, we measured total contents of phenols, antioxidant effects, free sugars, organic acids, and chromaticity. During fermentation, no significant difference was observed between the C. indicum L. group and control group. Regarding total phenol contents and antioxidant effects, only the 5% and 10% C. indicum L. groups showed higher contents of phenols than the non-addition group. In general, 5% and 10% C. indicum L. addition groups were positively evaluated. In conclusion, Kukhwaju with 10% C. indicum L. extract showed the best antioxidant effects. In the present study, we obtained different characteristics and determined the optimum addition amount of C. indicum L.

Optimum Nutrient Concentration to Improve Growth and Quality of Strawberry Cultivars 'Berrystar' and 'Jukhyang' in Hydroponics (딸기 수경재배 시 '베리스타'와 '죽향'의 생육과 품질 향상을 위한 적정 양액농도 설정)

  • Choi, Su Hyun;Choi, Gyeong Lee;Jeong, Ho Jeong;Kim, Seung Yu;Lee, Seong Chan;Choi, Hyo Gil
    • Journal of Bio-Environment Control
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    • v.26 no.4
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    • pp.424-431
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    • 2017
  • This study was conducted to set the optimum nutrient solution concentration by growth stage for new strawberry cultivars 'Berrystar' and 'Jukhyang'(Fragaria ${\times}$ ananassa Duch. cvs. 'Berrystar', 'Jukhyang') grown through hydroponics to improve the quality and yield. Three different EC levels were applied to the nutrient solution. The treatment levels were 0.7, 1.0 and 1.3 times higher than the nutrient concentration standard for 'Seolhyang' based on the 'Manual for strawberry cultivation' of Rural Development Administration. Based on the results, there were no significant differences in growth of 'Berrystar' by EC level. 'Jukhyang' showed the most vigorous growth grown in 1.3 times higher nutrient concentration. While the growth of 'Berrystar' and 'Jukhyang' grown in higher EC level has leaves with more chlorophyll concentration. However the quantum yield of leaves was not affected by the treatments. On the treatment with 1.3 times higher EC level, the weight, length, width and firmness of 'Berrystar' and 'Jukhyang' were significantly high. The sugar contents of the harvest analyzed by HPLC did not differed particularly, but the percentage composition of reducing sugar and non-reducing sugar were presented differently depending on the treatments. Marketable fruit yield increased as nutrient concentration increases. However, there were no large differences by treatments. Meanwhile, 'Jukhyang' showed significant difference by nutrient concentration and had the largest yield for a treatment grown in 1.3 times higher EC level. Based on these results, it is recommended to provide the same nutrient solution concentration level to the nutrient concentration standard of 'Seolhyang' for 'Berrystar', and the 1.3 times higher level for 'Jukhyang'.

Production of Poly (3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Bacillus sp. EMK-5020 Using Makgeolli Lees Enzymatic Hydrolysate and Propionic Acid as Carbon Sources (막걸리 주박 가수분해 산물과 propionic acid를 탄소원으로 이용한 Bacillus sp. EML-5020 균주로부터 poly (3-hydroxybutyrate-co-3-hydroxyvalerate) 생합성)

  • Kwon, Kyungjin;Kim, Jong-Sik;Chung, Chung-Wook
    • Journal of Life Science
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    • v.32 no.7
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    • pp.510-522
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    • 2022
  • In this study, to biosynthesize PHA with properties more similar to polypropylene, a Bacillus sp. EMK-5020 strain that biosynthesized poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was isolated from soil. Bacillus sp. EMK-5020 strain biosynthesized PHBV containing 1.3% 3-hydroxyvalerate (3HV) using reducing sugar contained in Makgeolli lees enzymatic hydrolysate (MLEH) as a single carbon source. As the amount of propionic acid, which was added as a second carbon source, increased, the content of 3HV also increased. PHBV containing up to 48.6% of 3HV was synthesized when 1.0 g/l of propionic acid was added. Based on these results, the strain was cultured for 72 hr in a 3 l fermenter using reducing sugar in MLEH (20 g/l) and propionic acid (1 g/l) as the main and secondary carbon sources, respectively. As a result, 6.4 g/l DCW and 50 wt% of PHBV (MLEH-PHBV) containing 8.9% 3HV were biosynthesized. Through gel permeation chromatography and thermogravimetric analysis, it was confirmed that the average molecular weight and the decomposition temperature of MLEH-PHBV were 152 kDa and 273℃, respectively. In conclusion, the Bacillus sp. EMK-5020 strain could biosynthesize PHBV containing various 3HV fractions when MLEH and propionic acid were used as carbon sources, and PHBV-MLEH containing 8.9% 3HV was confirmed to have higher thermal stability than standard PHBV (8% 3HV).

Effects of Foliar Application of Ethychlozate Mixed with Calcium Formulae on a Fruit Quality of Satsuma Mandarin ('Miyagawa Wase') in Plastic Film House Cultivation (Ethychlozate와 Ca제제 혼용살포가 하우스밀감의 품질에 미치는 영향)

  • Kim, Yong Ho;Moon, Duck Young;Kim, Han Yong
    • Horticultural Science & Technology
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    • v.18 no.5
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    • pp.605-611
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    • 2000
  • This experiment was conducted to examine the effects of foliar application of ethychlozate and ethyclozate mixed with different calcium formulae (clef-non, suical, cell-bine, and calcium acetate monohydrate) on the fruit quality and peel puffing of 'Miyagawa Wase' satsuma mandarin cultivated in a plastic film house. Foliar application of ethychlozate mixed with clef-non or suical showed a result that the 'a' value of peel chromaticity was increased, which are supposed to accelerate peel coloration without peel puffing. The reducing sugar levels of fruits in control, ethychlozate, ethychlozate+celef-non, ethychlozate+suical, ethychlozate+cell-bine, and ethychlozate+calcium acetate monohydrate treatment were 4.98, 5.30, 5.59, 5.00, 5.20, and 4.27%, respectively. Especially, in the case of ethychlozate mixed with clef-non, the reducing sugar level was 0.61% higher than that of control. Sucrose and total sugar content also had a similar trend as that in the reducing sugar contents. The sugar contents of fruits in various ethychlozate treatments mixed with different calcium formulae except those in ethychlozate treatment or ethychlozate treatment mixed with calcium acetate monohydrate were higher than $12^{\circ}Brix$. Especially, the treatment of ethychlozate treatment mixed with clef-non showed the highest sugar content with $12.7^{\circ}Brix$. The ratio of soluble solids to acidity also showed the similar tendency, but there was no significant difference in acidity among the treatments.

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Migration and Retardation Properties of Uranium through a Rock Fracture in a Reducing Environment (환원환경에서 암반 균열을 통한 우라늄 이동 및 지연 특성)

  • Baik, Min-Hoon;Park, Chung-Kyun;Cho, Won-Jin
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.5 no.2
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    • pp.113-122
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    • 2007
  • In this study, uranium migration experiments have been performed using a natural groundwater and a granite core with natural fractures in a glove-box constructed to simulate an appropriate subsurface environment. Groundwater flow experiments using the non-sorbing anionic tracer Br were carried out to analyze the flow properties of groundwater through the fracture of the granite core. The result of the uranium migration experiment showed a breakthrough curve similar to that of the non-sorting Br. This result may imply that uranium migrates as anionic complexes through the rock fracture since uranium can form carbonate complexes at a given groundwater condition. The distribution coefficient $K_d$ of the uranium between the groundwater and the fracture filling material was obtained as low as 2.7 mL/g from a batch sorption experiment. This result agrees well with the result from the migration experiment, showing a faster elution of the uranium through the rock fracture. In order to analyze retardation properties of the uranium through the rock fracture, the retardation factor $R_d({\sim}16.2)$ was obtained by using the $K_d$ obtained from the batch sorption experiment and it was compared with the $R_d({\sim}14.3)$ obtained by using the result from the uranium migration experiment. The values obtained from the both experiments were very similar to each other. This reveals that the retardation of the uranium is mainly occurred by the fracture filling material when the uranium migrates through the fracture of a granite core.

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