• Clinical and Experimental Pediatrics
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• v.48 no.6
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• pp.649-654
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• 2005

Purpose : The present study examined how changes in cerebral hemodynamics in newborn piglets with bilirubin infusion can be evaluated by near infrared sepctroscopy(NIRS). Methods : Seventeen newborn piglets were randomly divided into the following three experimental groups : six in the control group(CG); seven in the bilirubin infusion group(BG), and four in the bilirubin infusion with 7-nitroindazole group(NG). To achieve the concentration of bilirubin above 20 mg/dL, we injected a bolus of 40 mg/kg of bilirubin intravenously, followed by 30 mg/kg/hr of bilirubin continuous intravenous infusion. All groups were monitored with cerebral hemodynamics using near infrared spectroscopy(NIRS) and their brain cortexes were harvested and the activities of $Na^+$, $K^+$-ATPase, level of conjugated dienes, ATP and phosphocreatine(PCr) were determined biochemically. Results : No changes took place in CG. In BG and NG, base excess, pH, and MABP decreased, and lactate level in blood increased. Cerebral $Na^+$, $K^+$-ATPase activity and ATP, PCr level in BG significantly decreased and conjugated dienes increased compared to CG. These abnormalities observed in the BG were significantly improved in the NG. In continuous NIRS monitoring, [$HbO_2$], [HbT], and [HbD] in BG were significantlly decreased compared to CG. However these abnormalities between NG and CG were not significantly different. There were no significant differences in $ScO_2$ between the study groups. Conclusion : Our study suggests cerebral hemodynamic changes could be monitored by non-invasive NIRS in newborn piglets with bilirubin infusion.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.74-74
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• 2001

난자 동결방법의 선별은 보다 효과적인 난자은행의 개발에 필수 불가결한 중요한 요소이다. 이전의 연구에서 마우스의 난자를 ethylene glycol과 electron microscope grid를 이용한 유리화 동결법으로 동결 융해한 결과 기존의 slow freezing 방법에서보다 높은 생존율과 배발달율이 나타남을 관찰하였다. 그러나 동결융해후의 난자는 방추사와 염색체의 이상성이 대조군에 비해 높은 빈도로 나타나 융해후의 배발달율을 감소시키는 것으로 보고되었다. 이에 본 연구에서는 유리화동결법동안 항동해제에 Cytoskeleton system을 안정화시키는 cytoskeleton stabilizer인 taxol을 첨가시킨후 동결시켰을때 생존율과 발달율을 개선시킬 수 있는지 알아보고자 본 실험을 시행하였다. ICR mouse의 성숙란을 채취하여 연구목적에 따라 taxol을 첨가시키지 않은 대조군과 첨가시킨 실험군으로 분류하였다. 동결방법은 난자를 1.5 M ethylene glycol (EG)에 2분 30초간 노출시킨후 5.5 M EG와 1 M sucrose가 첨가된 동결액에 20초간 노출시킨 후 Grid에 난자를 부착시킨후 직접 액체질소에 침지하여 동결하였다. 동결후 난자는 5단계로 융해를 실시한 후 정자와 체외수정을 시킨 후 수정된 난자는 modified P1 배약액에 124 h까지 발달율을 관찰하였고, 배양 후 발달된 배반포는 대조군과 실험군, 각각 4마리의 발정동기화된 recipient에 이식을 시행하였다. 배발달율은 대조군에 비해 실험군에서 4세포기 (48 vs. 84.4%), 8세포기 (34% vs. 70.6%), 상실배 (26% vs. 58.6%) 그리고 배반포 발달율은 (24% vs. 58.6%)로 높게 관찰되었다. 배아이식후 대조군과 실험군에서 각각 2 마리가 임신이되어 정상적인 산자를 분만하였다. 따라서 항동해제에 taxol의 첨가는 동결 융해후의 난자의 배발달율을 증진시킬 수 있었다..8%로 나타나 난할율 및 배반포 발생율에 있어서 융합조건에 따라 큰 차이는 없었으나 1.9㎸/cm, 30$\mu\textrm{s}$ 2회의 조건이 다른 조건들에 비하여 유의적으로 낮았다. 따라서, 체세포와 수핵란 세포질간의 융합율과 배반포 발생에 미치는 영향은 전압보다는 시간에 더 크게 받음을 알 수 있었으며, 이와 같은 결과에서 융합시 시간을 오래 주는 것보다 전압을 높이는 것이 수핵난자의 세포질에 상해를 줄이고 이후 배반포 발생에 유리할 것으로 사료되었다.면에서도 더욱 더 활발할 것으로 기대된다. 배란후 72시간째에 초음파진단기를 이용하여 난소의 난포발달을 조사한 결과 , 대조구와 bFF처리구에 비해 AI처리구에서 발달난포가 유의적으로 많은 것을 확인하였다. 이상과 같은 결과로, Anti-inhibin serum은 한우 자체에서 분비하는 Inhibin을 특이하게 억제하여 Inhibin에 의해 억제되는 FSH분비가 촉진됨으로써 난포발달과 estrogen의 농도가 촉진되는 것으로 사료되어 anti-inhibin serum이 한우의 과배란유기 효과가 있는 것으로 사료된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도성 물질로 증착시키지 않고, Silver Paint 에 시편을 접착하는 방법으로도 만족한 시험 결과를 얻을 수 있었다.째, 회복기 중에 일어나는 입자들의 유입은 자기폭풍의 지속시간을 연장시키는 경향을 보이며 큰 자기폭풍일수록 현저했다. 주상에서 관측된 이러한 특성은 서브스톰 확장기 활동이 자기폭풍의 발달과 밀접한 관계가 있음을 시사한다.se that were all low in two aspects, named "the Nonsignificant group". And the issues were high risk perception in general setting and

• Neonatal Medicine
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• v.17 no.2
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• pp.254-261
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• 2010

Little is known about neonatal mitochondrial disease, though mitochondrial metabolic disorders may often present in the neonatal period because of the high energy requirement of neonate. In newborn period, common presentations are not specific and the disease course may be rapid and fatal. In this study, we report three cases of neonatal mitochondrial disease. The first case was strongly suspected because of sudden seizure and mental change with severe lactic acidosis, and multiorgan failure. Plasma lactate/pyruvate (L/P) ratio was increased to 55.6 with marked lactic aciduria and increased plasma alanin up to 2,237 nmol/mL. In the second patient, a peritoneal dialysis was performed for acute adrenal and renal failure, but metabolic acidosis persisted. Plasma L/P ratio was increased to 23.9, and MRC I (mitochondrial respiratory chain defect) was diagnosed through the enzymatic analysis of the muscles. The third case showed repetitive episode of lactic acidosis during the first two months of life, hypotonia, failure to thrive and feeding difficulties. We found markedly increased cerebrospinal fluid L/P ratio up to 57 though plasma L/P ratio(19.4) was borderline with increased plasma lactate. The lactate peak was prominent in brain magnetic resonance spectroscopy (MRS). MRC II was confirmed through muscle biopsy. Plasma lactate level and lactate peak of brain MRS were normalized after conservative treatment.

• Journal of the Korean Magnetics Society
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• v.26 no.3
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• pp.99-104
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• 2016

For patients receiving chemotherapy and radiation therapy treatment progresses as vomiting, nausea, weight of the patient because of a loss of appetite it is reduced. The patient's weight and the distance from the skin and the treatment site is expected to be closer, thereby reducing the change in the skin because of this dose. This study tests using a loose see the difference between the volume change appears as the weight of the patient using the same phantom and the phantom body of the patient. To using the same as the position EBT film is attached to the skin of the treatment site and was adjusted to the thickness of the Bolus. And using a computerized treatment planning only tomotherapy equipment was passed under the conditions according to the thickness of the radiation dose. To baseline for accurate reproduction position using the MVCT was applied to treated with verification. By passing a total of three dose reduced the error, it was a measure of the film by using a dedicated scanner, EBT VIDAR scanner. Got an increase in the skin dose is displayed each time the thickness of the bolus reduced, in a bolus was completely removed with the highest value. If the changes appeared dose was greater weight loss patients to chemotherapy and therefore bolus thickness variation considering the weight loss of the patient when applying the tomotherapy of nasopharynx cancer was found that the increase in skin dose be increased. This large patient before treatment due to weight loss over the image verification is considered to be established should consider how to re-create your mask and treatment plan for fixing it.

• Geotechnical Engineering
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• v.12 no.5
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• pp.17-26
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• 1996

The undrained behavior of Asan marine soil was investigated by using an automated triaxial testing device. The stress-strain behavior at the preand postfailure state of marine soil under undrained compression and eatension conditions was compared with the behavior of pure silt, pure clay and the overall behavior of Asan marine soil was predicted with the modified Camflay model and the bounding surface model. The marine soil sampled in Asan bay area was clayey silts with 70oA silt-30% clay content and the testing samples were prepared in both undisturbed and remolded conditions. All samples are normally consolidated with 400 kPa of effective mean confining pressure and each sample is unloaded to 200, 100, 67 kPa, respectively. And then the shear test was performed with different confining pressure. According to experimental results, there exists an unique failure line whose slope is lower than silt's and higher than clay's. It is identified that the undrained shear strength of normally consolidated samples increases after crossing the phase transformation line because of volume dilation tendency which is not seen in clay. Overconsolidated samples show different soil behavior compared with pure silt due to its tendency of change in volume. It is also found that the overall behavior of Asan marine soil cannot be predicted precisely with the modified Cam-clay model and the bounding surface model.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.2
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• pp.123-131
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• 2009

This study was carried out to investigate the feasibility for the use of environmental-friendly materials and the effective recycling of spent mushroom compost(SMC) after cultivation of Button Mushroom, Agaricus bisporus. SMC of white button mushroom contained diverse microorganisms including fluorescent Pseudomonas sp., Bacillus sp., Tricoderma sp. and Actinomycetes. These isolates showed the extensive antifungal spectrum against plant pathogen. Among of the isolates, fungal pathogen such as Alternaria brassicicola, Phytophtora melonis, Phytophthora capsici and Colletotichum gloeosporioides strong showed strong antagonistic activity. 45.8% of the isolates were actively colonized on the pepper root and 5.8% showed rhizosphere competent of >$5{\times}10^2cfu\;root^{-1}$. The plant growth promotion ability of the collected isolates were tested in pot experiments using red pepper seedling. Among them, 62.7% showed pepper growth promoting ability and growth of pepper root showed superior to the control. The germination of pepper treated with aqueous extracts of non-harvest SMC completely inhibited at concentration of more than 33%. The sterilization of SMC resulted in higher inhibition of germination and early growth of pepper. These results suggest that spent mushroom compost(SMC) of Button Mushroom may have adequately the feasibility for the use with environmental-friendly materials.

• Proceedings of the Korea Water Resources Association Conference
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• 2004.05b
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• pp.777-783
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• 2004

하천 세내지 주변은 급속한 시가지 조성과 인구밀집으로 유역의 불투수층이 증가하여 홍수도달시간이 짧아지고 홍수유출량이 증가하고 있다. 또한 엘리뇨${\cdot}$라니냐 등의 이상기후로 홍수사상의 발생 빈도와 규모가 증가하면서 홍수피해도 대형화되어 가고 있다. 그러나 치수사업은 다른 공공사업에 비해 경제성이 저평가 되어 투자우선순위가 밀려 사업시행이 지연되고 예방적 차원의 대책도 미흡하여 피해가 증가하는 악순환이 계속되고 있다. 따라서 본 인구에서는 우리나라의 치수경제성 분석에 있어 계량화하지 못하고 있는 자산이용고도화 효과를 치수안전도와 더불어 분석하고자 한다. 자산이용고도화는 치수사업 시행으로 해당지역의 치수안전도 향상에 따른 자산가치의 상승을 말하는데, 특정지역의 자산가치를 가장 객관적으로 표현할 수 있는 공시지가를 근거로 분석을 수행하였다. 치수사업 시행으로 인한 편익과 하천 특성에 따른 지가변동률의 차이가 통계적으로 유의성이 있는지를 분산분석을 통해 검증하였으면, 자산가치의 상승을 순수 연평균지가변동률로 나타내었다. 치수안전도는 홍수피해 잠재성과 홍수방어능력으로 구분하였는데 홍수피해 잠재성은 도시화율에 따라 구분하였고, 홍수방어능력은 홍수량의 빈도해석과 불확실성을 고려하여 조건부 비초과확률로 나타내었다. 본 연구에서는 소도시 지역(경안천, 복하천, 청미천)을 대상으로 200년 빈도의 홍수사상에 내해 10년, 50년 설계빈도로 건설된 제방의 조건부 비초과확률을 산정하여 지가변동률의 추이를 비교 분석하였다. 분석 결과, 소도시 지역에서는 조건부 비초과확률이 $10\%$ 상승했을 때 순수 연평균지가변동률이 5배정도 상승함을 알 수 있었다.다시 입력자료로 사용하는 업데이트 방식을 사용하기 때문에 예측결과의 오차가 완전하게 보정되지 않으면 다음 결과에 역시 오차를 주게 되어 오차보정이 상당히 중요하다는 것을 알 수 있었다. 오차를 보다 효과적으로 보정하기 위해서는 퍼지제어에 사용되는 퍼지규칙의 수를 늘리고, 유입량에 직접적인 영향을 주는 강우량과 연계한 2변수의 Fuzzy-Grey 모형을 이용한다면 보다 정확한 유입량 예측이 가능할 것으로 사료된다.이 작은 오차를 발생하였으며, 전체적으로 퍼프 모형이 입자모형보다는 훨씬 적은 수의 계산을 통해서도 작은 오차를 나타낼 수 있다는 것을 알 수 있었다. 그러나 Gaussian 분포를 갖는 퍼프모형은 전단흐름에서의 긴 유선형 농도분포를 모의할 수 없었고, 이에 관한 오차는 전단계수가 증가함에 따라 비선형적으로 증가하였다. 향후, 보다 다양한 흐름영역에서 장${\cdot}$단점 분석 및 오차해석을 수행한 후에 각각의 Lagrangian 모형의 장점만을 갖는 모형결합 방법을 제시할 수 있을 것으로 판단된다.mm/$m^{2}$로 감소한 소견을 보였다. 승모판 성형술은 전 승모판엽 탈출증이 있는 두 환아에서 동시에 시행하였다. 수술 후 1년 내 시행한 심초음파에서 모든 환아에서 단지 경등도 이하의 승모판 폐쇄 부전 소견을 보였다. 수술 후 조기 사망은 없었으며, 합병증으로는 유미흉이 한 명에서 있었다. 술 후 10개월째 허혈성 확장성 심근증이 호전되지 않아 Dor 술식을 시행한 후 사망한 예를 제외한 나머지 6명은 특이 증상 없이 정상 생활 중이다 결론: 좌관상동맥 페동맥이상 기시증은 드물기는 하나, 영유아기에 심근경색 및 허혈성 심근증 또는 선천성 승모

• Journal of Oral Medicine and Pain
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• v.34 no.4
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• pp.363-369
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• 2009

Erosive oral lichen planus (EOLP) and recurrent aphthous stomatitis (RAS) are T-cell mediated inflammatory immune disorders. It was investigated mRNA expression pattern of several regulatory factors, such as, CD28, CD45, CD152, CD154, CD279, which influence T lymphocyte in unstimulated whole saliva (UWS) of EOLP and RAS patients. It was collected unstimulated whole saliva during 10 minute in EOLP 18 people, RAS patients 12 people, healthy control 8 people. We investigated mRNA expression of T lymphocyte regulatory factors, such as, CD28, CD45, CD152, CD154, CD279, with real time reverse transcription polymerase chain reaction. In EOLP group, CD45, CD279 expressed higher and CD154 expressed lower than control. In RAS, CD45, CD270 expressed higher and CD28, CD154 expressed lower than control. In addition CD152 salivary mRNA expression of EOLP is higher than that of RAS. The above results were suggested that the mRNA expression of T lymphocyte regulatory factors in unstimulated whole saliva of EOLP and RAS contributes to diagnosis of diseases.

• Journal of the Korean Geotechnical Society
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• v.22 no.8
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• pp.77-88
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• 2006

In this study, the lateral behavior of Pile-Bent structures subjected to lateral loading was evaluated by a load-transfer approach. An analytical method based on the Beam-Column model and nonlinear load transfer curve method was proposed to consider material non-linearity (elastic and yielding) and $P-{\Delta}$ effect. Special attention was given to the lateral deflection of Pile-Bent structures depending on different soil properties, lateral load, slenderness ratio based on pier length and reinforcing effect of casing. From the results of the parametric study, it is shown that the increase of lateral displacement in a pile is much less favorable for an inelastic analysis than for an elastic analysis. It is found that for inelastic analysis, the maximum bending moment is located within a depth approximately 3.5D(D: pile diameter) below ground surface, but within 1.5D when $P-{\Delta}$ effect is considered. It is also found that the magnitude and distribution of the lateral deflections and bending moments on a pile are highly influenced by the inelastic analysis and $P-{\Delta}$ effect, let alone soil properties around an embedded pile.

• Korean Journal of Psychosomatic Medicine
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• v.10 no.1
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• pp.8-17
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• 2002

Objective : Increasing in frequency and success of hematopoietic stem cell transplantation and improved survival rates have led to growing concerns regarding the psychosocial aspects of hematopoietic stem cell transplantation recipients. In this study, we have examined the stress coping strategies and related psychiatric symptom in the hematopoietic stem cell transplantation recipients. Methods : In this study, we examined the psychological stress symptoms of hematopoietic stem cell transplantation recipients and differences of psychosocial variables between active coping group and passive coping group. Twenty nine recipients of hematopoietic stem cell transplantation were recruited prospectively and assessed at 2 weeks pretranplant and at 1-2 days posttranplant. Thirty normal controls were recruited. Assessments included a psychiatric interview, a variety of standardized questionnaires (Ways of Coping Questionnaires, Perceived Stress Scale, Hospital Depression and Anxiety Scale, Short-Form 36 Health Survey). Results : Hematopoietic stem cell transplantation patients showed higher degree of depression (p<0.001) and anxiety (p=0.011) symptoms than normal control group. However, no differences of depression and anxiety symptoms between pretransplant and posttransplant status were showed. And, passive coping group showed higher degree of depression (p=0.046) and anxiety symptoms (p<0.001) than active coping group. Conclusions : Our results suggested that many hematopoietic stem cell transplantation recipients would exhibit severe to moderate symptoms of anxiety and depression. Also, it seemed likely that passive coping style might influence the development of negative affect such as anxiety and depression. The implications of these findings were discussed in terms of the need to monitor the coping strategies and apply the appropriate psychiatric intervention. And, further prospective studies about long-term survival and psychological adaptive functions of hematopoietic stem cell transplantation patients are recommended.