• Korean Journal of Ecology and Environment
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• v.47 no.3
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• pp.160-166
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• 2014

This study was aimed at looking into the distribution status and age structure of Abies holophylla population in Sudo-Am temple forest. It was found that a total of 302 individuals of Abies holophylla existed which were more than 2m in height within the study area. Furthermore the population size is one of the largest in the southern region of Korea. The CBH of Abies holophylla ranged from 1.5 cm to 500.8 cm. Age structure of Abies holophylla looks like a gourd-shaped bottle. This means that they have an unstable structure status and do not survive very long. This status results from a variety of factors including, vegetation succession, anthropogenic activities, and global warming. The environmental characteristics of Abies holophylla population was $931{\pm}64.5m$ in mean altitude, $19.2{\pm}8.7^{\circ}$ in mean slope in the northeastern and southeastern area of the slope direction, and $1,324,323{\pm}174,459wh\;m^{-2}$ in average of direct normal irradiation. Among the site environmental factors, the significant ones which influence the potential habitat for Abies holophylla distribution were chosen using the MaxEnt model. According to the results of this study, altitude and slope were found as the important factors. The average value of environmental conditions by ROC analysis were altitude 903.2 m, slope $20.04^{\circ}$, irradiation $1,352.248wh\;m^{-2}$, and the southeastern aspect.

• Tunnel and Underground Space
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• v.22 no.4
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• pp.266-275
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• 2012

In this study, a method is devised to implement a supercritical $CO_2$ ($scCO_2$) injection environment on a laboratory scale and to investigate the effects of $scCO_2$ on the properties of rock specimens. Specimens of shale and sandstone normally constituting the cap rock and reservoir rock, respectively, were kept in a laboratory reactor chamber with $scCO_2$ for two weeks. From this stage, a chemical reaction between rock surface and the $scCO_2$ was induced. The effect of saline water was also investigated by comparing three conditions ($scCO_2$-rock, $scCO_2-H_2O$-rock and $scCO_2$-brine(1M)-rock). Finally, we checked the changes in the properties before and after the reaction by destructive and nondestructive testing procedures. The swelling of shale was a main concern in this case. The experimental results suggested that $scCO_2$ has a greater effect on the swelling of the shale than pure water and brine. It was also observed that the largest swelling displacement of shale occurred after a reaction with the $H_2O-scCO_2$ solution. The results of a series of the destructive and nondestructive tests indicate that although each of the property changes of the rock differed depending on the reaction conditions, the $H_2O-scCO_2$ solution had the greatest effect. In this study, shale was highly sensitive to the reaction conditions. These results provide fundamental information pertaining to the stability of $CO_2$ storage sites due to physical and chemical reactions between the rocks in these sites and $scCO_2$.

• Korean Journal of Environment and Ecology
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• v.35 no.1
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• pp.48-67
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• 2021

Plant species exhibit current characteristics as a result of interactions with environmental conditions. The plants of Viola sp. have selected chasmogamous flowers with vigorous vegetative propagation or development of cleistogamous flowers as an adaptation strategy. Viola websteri is distributed on the Korean peninsula and the eastern part of Jilin Province, China. The center and edge of the distribution are expected to exhibit different population-dynamics. It is necessary to investigate the cause of its current limited distribution even though V. websteri has a mixed-mating strategy. Firstly, We examined the vegetation environment of habitats and evaluated its characteristics. Growth characteristics were examined through plant phenology. We then evaluated the population structure, characteristics of chasmogamous flowers, and productivity of cleistogamous flowers. Moreover, we compared population sizes between 2014 and 2018. Most habitats were located in deciduous broadleaf mixed forests adjacent to valleys. V. websteri produced chasmogamous flowers with self-incompatibility in April-May and cleistogamous flowers in June-September. The cleistogamous flower production is a strategy ensuring seed production under uncertain environmental fluctuations; these were approximately twice as numerous as chasmogamous flowers. The population structure was distinguished into stable and very unstable regions. There were sites where the population experienced a sharp decline in the 2018 compared to that of 2014. This large decline was found in the edge populations. The habitats had different microsites depending on the natural disturbances of drought and the matrix constituting the habitat, thus supporting various plants. Ensuring the production of seeds through cleistogamous flowers, it was determined that rapid seedling re-establishment and population replenishment were possible when the natural disturbance factor was removed. Environmental factors did not equally affect all populations or individuals. Therefore, it was expected that it would be able to persisted in a long time, despite the rapid decrease in the number of individuals in the population regionally. Local extinction and re-establishment are likely to repeat according to environmental change. We propose the additional population investigation based on this works are required. We also suggest a need to assess the long-term population dynamics and the genetic characteristics of chasmogamous flowers and cleistogamous flowers to establish and implement effective conservation strategies.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.49 no.4
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• pp.313-321
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• 2023

Platycodon grandiflorus (P. grandiflorus) flower is a perennial plant belonging to the family Campanulaceae and has many excellent pharmacological effects, so it has been used as a medicinal ingredient since ancient times. In addition, anthocyanin is a purple or blue natural pigment contained in plant flowers and fruits, and is known as a powerful antioxidant. The purpose of this study was to confirm the dermatological functionality of P. grandiflorus flower extract and the value of the bluish anthocyanin contained in flowers as a cosmetic material as a natural pigment. Firstly, 50% ethanol and 80% ethanol were added to the P. grandiflorus flower and extracted under reflux for 4 h at 25, 60, and 80 ℃, and the pH of each treatment group was similar. Based on the anthocyanin content and chromaticity (E^*ab), 50% ethanol 60 ℃ extraction conditions showing the color development most similar to the natural color of the P. grandifloras flower were selected, and a sample was prepared by concentrating and lyophilizing. The analysis results showed that the total phenol, total flavonoid, and total anthocyanin contents were in the ranges of 23 ㎍/mL, 16 ㎍/mL, and 0.17 ㎍/mL, respectively. The P. grandiflorus flower extract suppressed the production of nitric oxide (NO) and interleukin-6 (IL-6) in lipopolysaccharide (LPS) induced RAW264.7 cells. Furthermore, the P. grandiflorus flower extract showed wound healing effects through the promotion of skin cell migration in TNF-α stimulated human keratinocytes. The stability of anthocyanin and extract color was studied during a storage period of 50 days at various temperatures (4 ℃, 25 ℃, and 45 ℃). Color values (L, a, and b) of the P. grandiflorus flower extract changed over 50 days, whereas the bluish-purple color of the extract was stabilized using 5% maltodextrin. These results suggest that P. grandiflorus flower extract may be useful as a natural cosmetic pigment.

• Journal of Sericultural and Entomological Science
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• v.16 no.2
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• pp.1-34
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• 1974

These experiments pertain to various factors influencing the quantitative characters of cocoon crops in summer and early autumn seasons. Initially, in order to establish the possible ways of the silkworm rearing more than three times a year in Korea, the author attempted to get further information about the various factors affecting the cocoon crop in every silkworm rearing season. The trials were conducted eleven times a year at four places for three years. The field trial was conducted with 19 typical sericultural farmers who had been surveyed. At the same time the author statistically analyzed the various factors in close relation to tile cocoon crop in autumn season. The effect of guidance on 40 sericultural farmers was analyzed, comparing higher level farmers with lower level farmers ; and the author surveyed 758 non-guided farmers near the guided farmers during both spring and autumn seasons. In addition, another trial on the seasonal change of leaf quality was attempted with artificial diets prepared with leaves grown in each season. It was found that related factors to cocoon crops in summer and early autumn seasons appeared to be leaf quality, and temperature for young and grown larvae. A 2$^4$ factorial experiment was designed in summer season, and another design with one more level of varied temperature or hard leaf added to a 24 factorial experiment was conducted in early autumn. The experimental results can be summarized： 1. Study on the cocoon crops in the different rearing seasons 1) It was shown that earlier brushing of silkworm generally produced the most abundant cocoon crop in spring season, and earlier or later than the conventional brushing season, especially earlier brushing was unfavorable for the abundant cocoon crop in autumn season. 2) The cocoon crop was affected by the rearing season, and decreases in order of sire with spring, autumn, late autumn, summer and early autumn seasons. 3) It was Proved that ordinary rearing and branch rearing were possibles 4 times a year ; in the 1st, 3rd, 8th, and 10th brushing season. But the 11th brushing season was more favorable for the most abundant cocoon crop of branch rearing, instead of the 10th brushing season with ordinary rearing. 2. Study on the main factors affecting the cocoon crop in autumn season 1) Accumulated pathogens were a lethal factor leading to a bad cocoon crop through neglect of disinfection of rearing room and instruments. 2) Additional factors leading to a poor cocoon crop were unfavorable for rearing temperature and humidity, dense population, poor choice of moderately ripened leaf, and poor feeding techniques. However, it seemed that there was no relationship between the cocoon crop and management of farm. 3) The percentage of cocoon shell seemed to be mostly affected by leaf quality, and secondarily affected by the accumulation of pathogens. 3. Study on the effect of guidance on rearing techniques 1) The guided farms produced an average yearly yield of 29.0kg of cocoons, which varied from 32.3kg to 25.817g of cocoon yield per box in spring versus autumn, respectively. Those figures indicated an annual average increase of 26% of cocoon yield over yields of non-guided farmers. An increase of 20% of cocoon yield in spring and 35% of cocoon yield in autumn were responsible. 2) On guided farms 77.1 and 83.7% of total cocoon yields in the spring and autumn seasons, respectively, exceeded 3rd grade. This amounted to increases of 14.1 and 11.3% in cocoon yield and quality over those of non-guided farms. 3) The average annual cocoon yield on guided farms was 28.9kg per box, based on a range of 31.2kg to 26.9kg per box in spring and autumn seasons, respectively. This represented an 8% increase in cocoon yield on farms one year after guidance, as opposed to non-guided farms. This yield increase was due to 3 and 16% cocoon yield increases in spring and autumn crops. 4) Guidance had no effect on higher level farms, but was responsible for 19% of the increases in production on lower level farms. 4. Study on the seasonal change of leaf quality 1) In tests with grown larvae, leaves of tile spring crop incorporated in artificial diets produced the best cocoon crop; followed by leaves of the late autumn, summer, autumn, and early autumn crops. 2) The cocoon crop for young larvae as well as for grown larvae varied with the season of leaf used. 5. Study on factors affecting the cocoon crops in summer and early autumn A. Early autumn season 1) Survival rate and cocoon yield were significantly decreased at high rearing temperatures for young larvae 2) Survival rate, cocoon yield, and cocoon quality were adversely affected by high rearing temperatures for grown larvae. Therefore increases of cocoon quantity and improvement of cocoon quality are dependent on maintaining optimum temperatures. 3) Decreases in individual cocoon weight and longer larval periods resulted with feeding of soft leaf and hard leaf to young larvae, but the survival rate, cocoon yield and weight of cocoon shell were not influenced. 4) Cocoon yield and cocoon quality were influenced by feeding of hard leaf to grown larvae, but survival rate was not influenced by the feeding of soft leaf and hard leaf. 5) When grown larvae were inevitably raised at varied temperatures, application of varied temperature in the raising of both young and grown larvae was desirable. Further research concerning this matter must be considered. B. Summer season 1) Cocoon yield and single cocoon weight were decreased at high temperatures for young larvae and survival rate was also affected. 2) Cocoon yield, survival rate. and cocoon quality were considerably decreased at high rearing temperatures for grown larval stages.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.90-91
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• 2003

어류 등의 종묘 생산시 초기 먹이로 이용되는 rotifer의 배양에 주로 해수산 Chlorella가 많이 이용되어 왔으나, 해수산 Chlorella는 수온 30℃이상 이거나, 10℃ 이하 일 때는 그 성장 상태가 극히 불안정하다. 따라서 부경대학교 한국 해양미세조류은행에서 보유하고 있는 국내외 Chlorella 종류 130종 중에서 채집 해역, 크기 등을 고려하여 해수산 Chlorella 5종, 해수산 Nannochloris 5종, 기수산과 담수산 Chlorella 각각 3종, 총 16종을 선택하여, 계절별 rotifer 배양에 적합한 Chlorella를 개발하고자 하였다. 먼저, 5종의 해수산 Chlorella를 수온 25℃, 염분 15‰과 30‰, 조도 5,000 lux 연속조명하에서 10일간 배양한 결과 15‰에서는 C-23 Chlorella vulgaris (감천), C-12 Chlorella vulgaris(낙동), C-20 Chlorella ellipsoidea(일본) 의 S.G.R. 이 각각 0.6621, 06353과 0.6251로 높게 나타났으며, 30‰에서는 C-23 이 13,146×10⁴cells/㎖로 가장 높은 세포 밀도를 보였다. 다음으로 5종의 해수산 Nannochloris를 동일한 조건에서 7일간 배양한 결과, 15와 30‰ 모두에서 C-31 Wannochloris oculata(UTEX), C-87 Nannochloris sp.(득량만) 와 C-189 Nannochloris sp.(부안) 의 S.G.R.이 0.9504∼0.9734로서 높은 성장률을 나타내었고, C-31은 30‰에서 11,229×10⁴cells/㎖의 높은 세포 밀도를 보였다. 그리고, 3종의 기수산 Chlorella는 25℃, 5,000 lux 연속조명, 염분 0, 15, 30‰에서, 담수산 3종은 동일한 수온과 조도에서 0, 15‰에서 각각 7일간 배양한 결과, 기수산에서는 S.G.R.이 0.6915∼0.8601로 나타났다. 특히, EC-001 Chlorella vulgaris(화진포)가 15와 30‰에서 각각 6491×10⁴cells/㎖, 6166×10⁴cells/㎖로 가장 높은 세포 밀도를 보였으며, 3종의 담수산 Chlorella는 0‰에서 S.G.R.이 0.6215∼0.6596의 성장률로 FC-001 이 2454×10⁴cells/㎖로 높은 밀도를 보였으나, 15‰에서는 모두 접종후 세포수가 감소하였다. 따라서 담수산 Chlorella는 rotifer의 적정 배양 염분인 15‰에서는 성장이 저조한 것으로 판단되어 제외하였다. 위의 배양 실험에서 각각 성장이 우수한 7종(C-12, C-20, C-23, C-31, C-87, C-189, EC-001)을 선정하여 다시 25℃, 15‰, 5,000 lux 연속 조명하에서 동시에 배양한 결과 해수산에서는 C-12, C-20, C-31, C-87 및 C-189의 S.G.R.이 0.8170∼0.8752로 높았으나, C-23은 0.7868로 낮게 나타났으며, EC-001은 0.7807로서 해수산에 비해서는 다소 낮았다. 한편, 이들 7종의 일반 성분을 분석한 결과에서는 조단백질은 C-31, C-12이 각각 42.93%, 42.7%였으며, 조지방 함량은 C-12 2.64%, C-31 2.58% 및 EC-001 2.43%로 나타났다. 위의 결과에서 C-23을 제외한 6종을 대상으로 성장과 영양성분이 높은 6종(C-12, C-20, C-31, C-87, C-189, EC-001)을 선택하여, 고수온기에 해당하는 32℃와 30℃, 저수온기인 10℃에서 각 종의 성장을 측정한 결과 32℃에서는 C-87과 C-189의 세포수가 6475×10⁴cells/㎖와 5932×10⁴cells/㎖로 가장 높았으며, 30℃에서는 C-31과 C-87이 각각 7951×10⁴cells/㎖와 7775×10⁴cells/㎖로 높게 나타났다. 반면 10℃에서 배양한 결과 EC-001이 3316×10⁴cells/㎖ 로 다른 종들의 107∼986×10⁴cells/㎖에 비하여 월등히 높은 세포 밀도를 나타내었다. 이들 6종의 미세조류를 L-type rotifer, Brachionus plicatilis에 먹이로 공급한 결과 C-12에서 5일째 300개체/㎖로 가장 높은 개체수를 나타내었다. 이와 같은 결과를 종합할 때 rotifer의 먹이로서는 여름철 고수온기에는 C-87 Nannochloris sp. (득량만)과 C-189 Nannochloris sp.(부안)이, 저수온기에는 기수산인 EC-001 Chlorella vulgaris(화진포)가 적당하며, 봄, 가을의 다른 계절에는 C-12 Chlorella vulgaris(낙동)이 가장 효과적일 것으로 판단되어진다.

• Microbiology and Biotechnology Letters
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• v.36 no.3
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• pp.215-220
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• 2008

Rahnella aquatilis AY2000 has an unique characteristic which produces an anti-yeast substance (AYS). The AYS of the strain AY2000 was always secreted on agar plate, however, its activity in liquid culture was labile upon storage of the medium. In this paper, cultural conditions of the strain AY2000 for the AYS production were investigated in liquid culture, and minimal inhibitory concentration (MIC) against Saccharomyces cerevisiae was determined for the AYS activity. MIC of the AYS cultured in PYG broth at $^25{\circ}C$ for 24 hr was $23.5{\mu}g/mL$, however, that in MYCS (pH 5.5) broth at the same condition was $15.5{\mu}g/mL$. The activity of the AYS had increased rather in MYCS broth excluded $NH_4$-citrate than in the same broth contained $NH_4$-citrate, and MIC of the AYS produced in MYCS broth without $NH_4$-citrate was $15.5{\mu}g/mL$. When the strain AY2000 was maintained in MYCS broth without $NH_4$-citrate but added $100{\mu}M$ $FeCl_3$, the activity of the AYS had increased and its MIC was $7.8{\mu}g/mL$. MIC of the AYS was $7.8{\mu}g/mL$ after the strain AY2000 was cultured in MYCS broth containing $100{\mu}M$ $FeCl_3$ without $NH_4$-citrate, however, its MIC was $31.3{\mu}g/mL$ after 48-60 hr culture in the same broth.

• Journal of Life Science
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• v.26 no.3
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• pp.376-386
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• 2016

Apoptosis induction has been proposed as an efficient mechanism by which malignant tumor cells can be removed following chemotherapy. The intrinsic mitochondria-dependent apoptotic pathway is frequently implicated in chemotherapy-induced tumor cell apoptosis. Since DNA-damaging agent (DDA)-induced apoptosis is mainly regulated by the tumor suppressor protein p53, and since more than half of clinical cancers possess inactive p53 mutants, microtubule-damaging agents (MDAs), of which apoptotic effect is mainly exerted via p53-independent routes, can be promising choice for cancer chemotherapy. Recently, we found that the apoptotic signaling pathway induced by MDAs (nocodazole, 17α-estradiol, or 2-methoxyestradiol) commonly proceeded through mitotic spindle defect-mediated prometaphase arrest, prolonged Cdk1 activation, and subsequent phosphorylation of Bcl-2, Mcl-1, and Bim in human acute leukemia Jurkat T cells. These microtubule damage-mediated alterations could render the cellular context susceptible to the onset of mitochondria-dependent apoptosis by triggering Bak activation, Δψm loss, and resultant caspase cascade activation. In contrast, when the MDA-induced Bak activation was inhibited by overexpression of anti-apoptotic Bcl-2 family proteins (Bcl-2 or Bcl-xL), the cells in prometaphase arrest failed to induce apoptosis, and instead underwent mitotic slippage and endoreduplication cycle, leading to formation of populations with 8N and 16N DNA content. These data indicate that cellular apoptogenic mechanism is critical for preventing polyploid formation following MDA treatment. Since the formation of polyploid cells, which are genetically unstable, may cause acquisition of therapy resistance and disease relapse, there is a growing interest in developing new combination chemotherapies to prevent polyploidization in tumors after MDA treatment.

• Korean Journal of Soil Science and Fertilizer
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• v.18 no.2
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• pp.121-127
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• 1985

This study was designed to gain practical data on the use of soil conditioners for more efficient water managements and to establish the optimum levels of structural properties for soil conditioning. A sandy loam and a silt loam soil were each treated with two different soil conditioners, hydrophobic Bitumen or hydrophilic Uresol. The perspex tube 34 cm long were packed homogeneously with air dried soil up to 2 cm below the top, then covered over 2 cm of treated or untreated aggregates. The infiltration rate into the soil columns was measured under simulated rainfall condition. The evaporation study was carried out in the wind tunnel, and the changes of soil moisture distribution of the columns following and during the evaporation were determined by a gamma ray scanner. The infiltration rate of water into the soil column was increased to 18.7-50.8% by the Uresol treatment but it was decreased to less than 25% of control by the Bitumen treatment. Evaporation was decreased to 22.0-68.1% by the Bitumen treatment and to 38.7-68.4% by the Uresol treatment. The water use efficiency of Uresol treated column was increased to more than twice as much as that of untreated soil. Aggregate stability and wetting angle were related to water infiltration and evaporation. A positive and highly significant logarismic relationship was found between the infiltration rate and stability index-wetting angle, evaporation rate and instability index-wetting angle. It was considered that the structural stability is more important than wetting angle. This is true because the structural stability is always positively correlated to water saving, however wettability is positively correlated to the infiltration, and negatively correlated to water saving during the evaporation.

• Journal of Institute of Control, Robotics and Systems
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• v.7 no.9
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• pp.717-722
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• 2001

This paper presents a methodology on the backward path tracking control of a trailer type robot which consists of two parts: a tractor and a trailer. It is difficult to control the motion of a trailer vehicle since its dynamics is non-holonomic. Therefore, in this paper, the modeling and parameter estimation of the system using a real-coded genetic algorithm(RCGA) is proposed and a backward path tracking control algorithm is then obtained based on the linearized model. Experimental results verify the effectiveness of the proposed method.