• Journal of dental hygiene science
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• v.10 no.4
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• pp.227-231
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• 2010

The purpose of this study was to examine the MAPK signaling pathways involved in regulation of HO-1 and the odontoblast differentiation markers during the odontoblastic differentiation for HDPCs. We evaluated cell growth by MTT assay and differentiation marker mRNA expression by RT-PCR. When the cells were treated with p38 inhibitor (SB203580, $10{\mu}M$), JNK inhibitor (SP600125, $10{\mu}M$), and ERK inhibitor (PD98059, $20{\mu}M$) for 7 days, cell growth and expression of HO-1 and differentiation makers were significantly decreased in HDPCs. Our results suggest that odontoblastic differentiation is positively regulated by HO-1 induction in HDPCs via ERK, JNK, and p38 signaling pathways. Thus, pharmacological HO-1 induction might represent a potent therapeutic approach for pulp capping and the regeneration of HDPCs.

• Journal of Korean Medical classics
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• v.20 no.1
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• pp.11-16
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• 2007

백고주요외표적골격이측량료인체전 후(後) 측면적골도(側面的骨度), 소이타수립료측량(所以他樹立了測量)"맥도(脈度)"的基礎(적기초). 병차타통과실지해부이요조도인체적실지구조, 저위적시인출이수곡능유지생명적생리학적기초(這爲的是認出以水穀能維持生命的生理學的基礎). 저양과정중백고주목료(這樣過程中伯高注目了), 수곡진입어위(水穀進入於胃), 타통과나리포산어전신, 여하이대소변배제(如何以大小便排除), 타인위상초여하초종상구여하구발출(他認爲上焦與下焦從上口與下口發出). 타우관심(他又關心), 대어오미진입어일양성질적장, 통과영(通過營)·위운행(衛運行), 저화상초(這和上焦)·중초련계(中焦聯系), 이차여하형식운행(而且如何形式運行). 우기백고장천인상응어위기운행적시간론(尤其伯高將天人相應於衛氣運行的時間論), 인차타계량화료위기적운행(因此他計量化了衛氣的運行). 타입족어천인상관론이강구료생리(他立足於天人相關論而講究了生理)·병리(病理)·진단급치료법(診斷及治遼法). 동시타입족어형(同時他立足於形)·기이분법적생명관(氣二分法的生命觀), 분류치병이제정자법(分類治病而制定刺法).

• Journal of the Korean Institute of Intelligent Systems
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• v.14 no.4
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• pp.402-410
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• 2004

In this paper, an optimal design method of clonal selection based Fuzzy-Neural Networks (FNN) model for complex and nonlinear systems is presented. The FNNs use the simplified inference as fuzzy inference method and Error Back Propagation Algorithm as learning rule. Also Advanced Clonal Selection (ACS) is proposed to find the parameters such as parameters of membership functions, learning rates and momentum coefficients. The proposed method is based on an Immune Algorithm (IA) using biological Immune System and The performance is improved by control of differentiation rate. Through that procedure, the antibodies are producted variously and the parameter of FNN are optimized by selecting method of antibody with the best affinity against antigens such as object function and limitation condition. To evaluate the performance of the proposed method, we use the time series data for gas furnace and traffic route choice process.

• Journal of Life Science
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• v.18 no.11
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• pp.1479-1484
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• 2008

Deep seawater (DSW) refers to water extracted from the ocean, usually at depths of 200 meters or more, which is rich in inorganic materials and has attracted attention for various applications. We investigated the effects of the DSW on the synaptic maturation of cultured rat hippocampal neurons. Immunocytochemical examination of DIV21 showed that PSD-95, $\alpha$CaMKII, and synGAP$\alpha1$clusters were strengthened and coupling rates of SV2 and NR2B were significantly increased in neurons grown in the presence of H-800 and H-1000 DSW. Our results indicate that DSW promotes the formation of excitatory postsynaptic signal transduction complexes NRC/MASC and functional synapses.

• Journal of Life Science
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• v.18 no.6
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• pp.897-901
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• 2008

Deep seawater (DSW; deep ocean water) is pure, rich in inorganic materials which have attracted attention for various applications. In this study we investigated the effects of the DSW upwelled from the East Sea, offshore Yang Yang (Korea) on the morphological differentiation of cultured rat hippocampal neurons, which were grown in the minimal essential medium containing 10% (v/v) fetal bovine serum and 25% (v/v) DSW with various hardness. DSW had no effect on initial morphological differentiation (17 hr post-plating). When observed on DIV3, 7, 14, and 17, low hardness (0 and 200) DSW reduced dendritic branching. However, dendritic branches within $80\;{\mu}m$ diameter from the center of soma nearly doubled in neurons grown in hardness 1,000 DSW-containing media. DSW with hardness 600 was more or less same as control groups. These results indicate that DSW with appropriate hardness ameliorates neuronal health.

• Journal of Life Science
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• v.21 no.9
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• pp.1346-1353
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• 2011

Sophora tonkinensis Gapnep has been used as a traditional herbal medicine in oriental regions since ancient times. In this study, the effect and mechanism of the MeOH extract of Sophora tonkinensis Gapnep (STME) on adipocite differentiation and adipogenesis in 3T3-L1 preadipocites were investigated. Treatment with STME in the concentration range of 0-200 ${\mu}g$/ml significantly inhibited the differentiation of 3T3-L1 preadipocites in a dose-dependent manner, as determined by a decrease in intracellular lipid droplets and lipid contents measured by Oil Red O staining. In association with the inhibitory effect of lipid accumulation, the expressions of the proteins concerned with adipogenesis in 3T3-L1 preadipocites were also investigated. Treatment with STME reduced the expressions of peroxisome proliferator-activated receptor ${\gamma}$ (PPAR${\gamma}$), cytidine-cytidine-adenosine-adenosine-thymine (CCAAT)/enhancer-binding proteins ${\alpha}$ and ${\beta}$ (C/EBP${\alpha}$ and C/EBP${\beta}$) and sterol regulatory element binding protein (SREBP), which are adipocyte specific markers. In flow cytometry analysis, the inhibitory effect of differentiation was caused by G1 arrest and following mitotic clonal expansion cease. Therefore, we also investigated the alteration of G1 phase arrest-related proteins. As a result, the expression of p21 protein was significantly increased, while the expressions of Cdk2, E2F-1 and phospho-Rb were reduced in a dose-dependent manner in STME treated 3T3-L1 cells. According to these results, STME might inhibit differentiation through G1 arrest in 3T3-L1 preadipocytes adipogenesis, and further studies, which are in progress, have to be completed to identify the active compounds.

• Journal of Life Science
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• v.32 no.12
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• pp.979-988
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• 2022

Halophytes have been reported to possess a variety of physiological activities, such as anti-cancer, anti-oxidant, anti-diabetes, anti-inflammatory, and anti-obesity. Studies on the roots of the halophyte Rosa rugosa, in particular, have shown a variety of physiological activities and are known to be effective for nursing diabetic complications in traditional Korean medicine. In this study, the effect of R. rugosa on adipogenesis was investigated in 3T3-L1 pre-adipocytes treated with crude extract and solvent fractions (H₂O, n-BuOH, 85% aq. MeOH, and n-Hex) obtained from R. rugosa roots. Treatment with extract and the solvent fractions inhibited the formation of intracellular lipid droplets in differentiated 3T3-L1 adipocytes compared to the untreated group. In particular, n-BuOH and 85% aq. MeOH fractions effectively decreased the expression of adipogenic transcription factors: peroxisome proliferator activated receptor-γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), and sterol regulatory element-binding protein 1c (SREBP1c) in both mRNA and protein levels. In conclusion, these results suggest that R. rugosa contains anti-adipogenic molecules that can be utilized as a nutraceutical against obesity. Further refining of n-BuOH and 85% aq. MeOH fractions and analysis of their action mechanisms could yield potential therapeutic agents with anti-adipogenic effects.

• Korean Journal of Ichthyology
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• v.2 no.1
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• pp.95-105
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• 1990

Gonadal sex differentiation of Misgurnus mizolepis was studied during growth from the size of 3.5 mm (2-day old) larvae to about 80 mm in total length (100-day-old) adult fish. One pair of primordial germ cells were found 2 days after hatching, and the development of prominent genital ridge was noticed at both stages of 4 days and 5 days after hatching. The gonadal sexes of this species were clearly distinguishable in 8 days, when the fry reached 15. 0 mm in total length. Spermatids first appaered at the stage of 20 days after hatching and 100-day-old female fish had well-developed ovaries. Above results indicate that M. mizolepis belongs to the differentiated type of gonochoristic teleosts.

• Development and Reproduction
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• v.2 no.2
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• pp.197-203
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• 1998

DNA methylation seems to play an important regulatory role in gene expression and cell differentiation during postimplantation embryonic development. However, the significance of DNA methylation which is maintained by the DNA MTase during preimplantation embryonic development, is not fully understood. In order to study the role of DNA methylation in the preimplantation embryos, the expression of DNA MTase transcripts was monitored in the oocytes and preimplantation embryos. The mRNA of DNA MTase was detected in the oocytes and pleimplantation embryos. The relative mRNA levels of DNA MTase were high from the stages of GV-oocytes and pronuclear embryos, and thereafter decreased gradually. By the treatment of $\alpha$-amanitin, it was confirmed that the transcripts presented in pronuclear embryos was derived from the maternal genome. The presence of transcripts of DNA MTase in the oocytes and pronuclear embryos suggests that the maintenance of DNA methylation may be necessary and seems to play an important role in gene expression and cell differentiation during preimplantation embryonic develop-ment in mouse.

• The Journal of the Petrological Society of Korea
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• v.8 no.2
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• pp.81-91
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• 1999

The area of the study is located in Ogcheon district, middle part of Ogcheon Fold Belt. The area is covered by metasedimentary rocks of Ogcheon Supergroup at northern, eastern and southern part. Jurassic Ogcheon granite which intruded into Ogcheon Supergroup at central part, was intruded by Cretaceous quartz porphyry at western part. The granite consists of quartz, plagioclase, alkali feldspar, biotite, sphene, apatite, epidote, opaque and so on. It is generally characterized by grey to light grey, medium-grained, mafic enclave and partly weak foliation. In terms of geochmical compositions, the granite is felsic, peraluminous, subalkaline and calc-alkaline, and it was differentiated from single granitic magma. It shows parallel LREE enrichment and HREE depletion patterns with 0.84 Eu negative anomaly, which has REE variation trend and anomaly value similar to Jurassic granites in Korea. From charactristics of petrology, mineralogy and geochmistry, it may be interpreted that the Ogcheon granite body was derived from melting of I-type crustal material related to syn-collisional tectonic setting and emplaced more or less rapidly into the Ogcheon Supergroup.