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http://dx.doi.org/10.5352/JLS.2008.18.11.1479

Promotion of Synaptic Maturation by Deep Seawater in Cultured Rat Hippocampal Neurons  

Kim, Seong-Ho (Department of Internal Medicine, Dongguk University)
Lee, Hyun-Sook (Department of Anatomy, College of Medicine, Dongguk University)
Shon, Yun-Hee (Regional Innovation System, Dongguk University)
Nam, Kyung-Soo (Regional Innovation System, Dongguk University)
Moon, Il-Soo (Department of Anatomy, College of Medicine Dongguk University)
Publication Information
Journal of Life Science / v.18, no.11, 2008 , pp. 1479-1484 More about this Journal
Abstract
Deep seawater (DSW) refers to water extracted from the ocean, usually at depths of 200 meters or more, which is rich in inorganic materials and has attracted attention for various applications. We investigated the effects of the DSW on the synaptic maturation of cultured rat hippocampal neurons. Immunocytochemical examination of DIV21 showed that PSD-95, $\alpha$CaMKII, and synGAP$\alpha1$clusters were strengthened and coupling rates of SV2 and NR2B were significantly increased in neurons grown in the presence of H-800 and H-1000 DSW. Our results indicate that DSW promotes the formation of excitatory postsynaptic signal transduction complexes NRC/MASC and functional synapses.
Keywords
CaMKII; deep seawater; hippocampal neuron; NR2B; SV2; synapse; synGAP$\alpha1$;
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