• Journal of Chest Surgery
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• v.39 no.7 s.264
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• pp.527-533
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• 2006

Background: A retrospective study was conducted to analyze the results of St. Jude Medical mitral valve replacement at the Chonbuk National University Hospital since the initial implant in May 1984. Material and Method: Between May of 1984 and December of 1996, 95 patients underwent MVR with the St. Jude Medical mechanical valve prosthesis at Department of Medical Science of Chonbuk National University Hospital and follow-up ended in May of 2004. Result: Age ranged from 19 to 69 years. Follow-up (mean${\pm}$standard deviation) averaged $10.6{\pm}4.2\;year$. Thirty-day operative mortality was 4.2% (4/95). Nine late deaths have occurred and actuarial survival was $90.5{\pm}3.0%,\;87.9{\pm}3.4%\;and\;83.2{\pm}4.6%$ at 5, 10 and 20 years, respectively. Probability of freedom from valve-rotated death was $95.5{\pm}2.1%,\;94.3{\pm}2.4%\;and\;91.0{\pm}3.9%$ at 5, 10 and 20 years, respectively. Seven patients have sustained thromboembolic events (1,05%/patient-year). Fifteen patients had anticoagulation related hemorrhage (3.56%/patient-year). There was no structural valve deterioration. Probability of freedom from all complications was $82.0{\pm}3.9%,\;71.3{\pm}4.8%\;and\;42.4{\pm}10.5%$ at 5, 10 and 20 years, respectively. Conclusion: We confirm the effective and excellent durability of the St. Jude Medical prosthesis in the mitral position with a low event rate at long-term follow-up. It also demonstrates the commonly encountered practical difficulty of adjusting the anti-coagulation protocol in patients with prosthetic mitral valves.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.4
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• pp.366-375
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• 1984

In order to confirm the effect of climatic consumption index (C C I) on the number of spikelets and yield of rice, 3 levels of shading rates such as 0, 25, 50% of full light were treated during the tillering stage, reproductive growth stage and ripening period, respectively, in a phytotron controlled with day/ night temperature of 20/10$^{\circ}C$ and 30/20$^{\circ}C$, and field at Crop Experiment Station, Suwon, Korea. The results are as follows: 1. As solar radiation decreased during the reproductive growth stage in 30/20$^{\circ}C$ or field condition, the number of spikelets per panicle was decreased due to the decrease of the number of differentiated secondary rachis branches and spikelets as well as the increase of the number of degenerated secondary rachis branches and spikelets. 2. Our results showed slight negative correlation between C C I of the reproductive growth stage and number of panicles per square meter and number of differentiated secondary rachis branches. On the other hand, there was highly significant positive correlation between C C I of the reproductive growth stage and the number of degenerated secondary rachis branches and spikelets, and negative correlation in number of differentiated spikelets. 3. The shading during the reproductive growth stage did not affect on the percentage of ripened grains and 1000 grains weight of hulled rice, whereas those were decreased with shading during the ripened period. 4. Influence of shading in each growing stage on the yield was severe in the order of ripened period, reproductive growth stage, tillering stage. 5. Respiration rate in Jinheung was higher than that of Tongil at low temperature, but reversed above 30$^{\circ}C$. Respiratory coefficients (Q$\sub$10/) of Tongil and Jinheung were 2.74 and 1.96, respectively. Respiration/ photosynthesis ratio in Jinheung was higher than that of Tongil at low temperature, while higher in Tongil above 32$^{\circ}C$. 6. Transportation of $\^$14/C was restricted at 20/10$^{\circ}C$ in Tongil, however, there was no differences at 30/20$^{\circ}C$ in both Tongil and Jinheung. The influence of shading on the transportation of $\^$14/C did not affect at 20/10$^{\circ}C$, but it was hampered with shading at 30/20$^{\circ}C$ in both varieties.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.1
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• pp.41-51
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• 1991

This study was conducted to determine the effect of 20 years long term fertilizations on the physical and chemical properties of paddy soil and the growth, yield, yield components and grain development of rice. Non-fertilized, PK, NK, NP, NPK, NPK + compost, NPK+straw and NPK+lime have been applied since 1968 after surface paddy soil was removed. NPK+compost and NPK+straw applications increased the content of organic matter, available P and CEC, and lime increased soil acidity and SiO$_2$ content. While chemical contents in non-fertilized treatment were low as compared with other treatments. Soil porosity was higher in NPK+straw (51.4%) and NPK+lime(53.1%) than in NPK application (49.8%). Soil hardness was highest in the NPK application and was lowest in the NPK + lime. Continuous application of straw with NPK markedly increased the content of aggregate with over 1mm(19.6%) as compared with NPK application (7.1%). Plant height, tiller number, root number, leaf area index and total dry weight were higher in the applications of compost, straw and lime with NPK than in any other treatments. Brown rice yield in non-fertilized, PK and NP applications was decreased 45, 55, 15 and 5% of that in NPK application, respectively, while application of compost, straw and lime with NPK increased the yield by 11, 14 and 4%, respectively, during 20 years. The number of differentiated rachis branchs in the application of compost, straw and lime was 17 to 21 and that in the other application was 13 to 15, whereas the degenerated rachis branchs was low in the application of compost, straw and lime with NPK. The applications having higher level of perfect rice grain such as non-fertilized, NPK+compost, NPK+straw and NPK+lime had high grain weight and had low level of white core rice, white belly rice. The white core and belly rice was highest in the NP application and notched belly rice kernel was markedly increased in NK and NP applications. The period of grain filling was 30 DAH at NP and NPK applications, 35 DAH at NK and NPK+lime, 40DAH at NPK+compost and NPK+ straw, and 45DAH at non-fertilized, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.5
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• pp.664-670
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• 2016

Cheonggukjang (CKJ) is a Korean traditional food made of fermented soybeans. In comparison to normal intake of soybeans, Cheonggukjang has high digestibility with bioactive, antioxidant substances, and thrombolytic enzymes. Recent studies have reported anti-oxidant, anti-cancer, anti-inflammatory, anti-obesity activities as well as inhibitory activities against osteoporosis for CKJ. In this study, we identified the effects of CKJ on osteoblast differentiation by increasing the polyglutamic acid (PGA) content of CKJ. Alkaline phosphatase (ALP) activity and mineralization significantly increased in response to treatment with both natural CKJ (CKJ A) and PGA-increased CKJ (CKJ B). However, CKJ B exhibited higher ALP activity and mineralization than CKJ A. Real-time reverse transcription PCR demonstrated that mRNA expression of osteoblastic-associated genes such as type I collagen, alkaline phosphatase, osteocalcin, and osteopontin in C2C12 cells was significantly up-regulated by CKJ A or B treatment. These results indicate that treatment with CKJ has an anabolic effect on bone by increasing osteoblastic differentiation and ALP activity. Increasing PGA content in CKJ had a greater effect than CKJ A on up-regulation of osteoblastic gene expression in osteoblast cells.

• Development and Reproduction
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• v.4 no.1
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• pp.95-100
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• 2000

A large number of man-made chemicals that have been released into the environment have the potential to disrupt the endocrine system of animals and humans. There is a critical developmental period during which sexual brain differentiation proceeds irreversibly under the influence of gonadal hormone. Recently we identified KAP3 gene expressed during the critical period of rat brain sexual differentiation. KAP3 functions as a microtubule-based motor that transports membranous organelles anterogradely in cells, including neurons. In the present study, we aimed to investigate the effect of endocrine disrupter, Dichlorodiphenyl trichloroethane (DDT), on the KAP3 gene expression during critical period of rat brain development. Maternal exposure to DDT increased the level of KAP3 mRNA in male and female fetus brains when examined on the gestational day 17 (GDl7). In postnatal day 6, DDT suppressed the expression of KAP3 gene in male and female rat brain. Also, the body weight and fertilization rate were decreased in the DDT exposured rats. These results showed that endocrine disrupter, DDT, can affect the transcriptional level of brain sexual differentiation related gene, KAP3, in the prenatal and the neonatal rat brain and that maternal exposure to endocrine disruptors may lead to a toxic response in embryonic differentiation of brain. And so KAP3 gene may be used a gene maker to analyse the molecular mechanism for toxic response in animal nerve tissues exposed to endocrine disruptors.

• Journal of Plant Biotechnology
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• v.43 no.4
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• pp.486-491
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• 2016

The aim of this research is to establish shoot regeneration system for 'Wonhwang' pear (Pyrus pyrifolia L.) using various concentrations of 1-Naphthaleneacetic acid (NAA) 0.01, 0.05, 0.1, 0.5 mg/L in combination with benzylaminopurine (BA) 3, 5, 10 mg/L. Medium containing 4.4 g/L of Murashige and Skoog (MS) medium with vitamins containing 8 g/L of plant agar and 30 g/L of sucrose with NAA 0.05 mg/L and BA 3 mg/L showed 13.3% of shoot regeneration rate. 'Wonhwang' showed no root growth on existing rooting media of P. pyrifolia cv. Niitaka, 'Whang-keumbae' and 'Bae Yun No. 3'. We evaluated the effect of concentration and kinds of plant growth regulators and carbon source to establish efficient rooting condition for 'Wonhwang' pear. In the result of using various concentrations of NAA 0.5 mg/L and 1.0 mg/L in combination with indolebutyric acid (IBA) 3, 5, 10 mg/L, rooting rate of 24% was observed using 1/4 Linsmaier and Skoog (LS) medium supplemented with 7.5 g/L glucose as carbon source and IBA 1.0 mg/L with NAA 1.0 mg/L.

• Journal of Applied Biological Chemistry
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• v.54 no.1
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• pp.26-32
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• 2011

To improve genetic transformation of Brassica napus winter cultivar 'Youngsan', factors influencing shoot regeneration and transformation from cotyledonary petioles were investigated. Shoot induction was enhanced in the combination of 0.5 mg/L NAA and 2~4 mg/L kinetin. Silver nitrate was essential for successful shoot regeneration, ranging from 5 to 9 mg/L. The addition of $GA_3$ promoted plant regeneration. Among the tested Agrobacterium strains, co-cultivation times, and antibiotic selection regimes, choice of appropriate Agrobacterium strain was the most critical factor for efficient transformation of B. napus cv. 'Youngsan'. The EHA105 succinamopine strain was the most efficient and the maximum transformation efficiency was 26.8%. Transgenic shoots were selected on 10 mg/L phosphinothricin (PPT) containing media. The transgenic plants expressing bar and gus genes were resistant for commercial herbicide "Basta" and stained with X-Gluc. Southern blot hybridization indicated that the presence of one to three gus gene copies per genome and inheritance of the gus gene into the $T_1$ generation.

• Korean Journal of Plant Tissue Culture
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• v.27 no.1
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• pp.1-6
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• 2000

This study was carried out to find the effects of plant growth regulators on callus formation, rooting and shooting from cotyledon explant in oriental me]on. Various combinations of 0.1 mg/L auxins (IAA, NAA) and 0.5, 1.0. 1.5, 2.0 mg/L cytokinins (BA, kinetin, zeatin) were treated to the MS basal medium, respectively. Callus was induced mort effectively as 2,437.0 mg (FW)/explant in MS medium supplemented with 0.1 mg/L NAA and 2.0 mg/L BA, but that was non-embryogenic callus as colored yellow white and broke easily. Root was induced most effectively at a frequency of 98.0% in MS medium supplemented with 0.1 mg/L NAA and 0-5 mg/L kinetin. Shoots formed on cut part of vein at a frequency of 98.0% in MS medium supplemented with 0.1 mg/L IAA and 2.0 mg/L BA, that were multiple shoots. in case of its concentration, BA and lower concentration of IAA and NAA (0.01 and 0.05 mg/L). respectively. shooting ratio was not increased. The result of treatment with BA 0-5 mg/L and IAA 0.1 mg/L, callus induced at a week, and shoot start to form multiple shoots about 3 weeks after inoculation. After 2 times subculture as 2 weeks intervals, divided shoots rooted and developed into intact plantlets at 10 weeks and then that grown normally on pots after acclimatization.

• Korean Journal of Plant Tissue Culture
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• v.27 no.2
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• pp.101-107
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• 2000

The explants of yacon (Polymnia sonchifolia Poeppig & Endlicher) were cultured to invest th8e dedifferentiation condition, and formative callus from leaf was cultured to find the regeneration and micro-crown bud formation. Basal MS medium was more effective to form callus than 1/2 MS and B$_{5}$ medium. Calli formations from leaf, petiole and lateral bud were more effective on MS medium supplemented with 1.0, 2.0 mg/L 2,4-D and 0.2, 0.4 mg/L kinetin or BA than 1.0, 2.0 mg/L NAA and 0.2, 0.4 mg/L kinetin or BA. Formative callus from leaf was proliferated about 70% on medium supplemented with 1.0 mg/L BA. When callus was proliferated, 63% regeneration rate was shown on medium supplemented with 1.0, 2.0 mg/L BA in case of subculture for 3~4 months but was not shown on medium supplemented with 1.0, 2.0 mg/L kinetin. Micro-crown bud formed as addition of BA at 3~4 months after callus culture and then was obtained many at 5~6 months, it was most formed about 82% on medium supplemented with 5 mg/L BA. Rate of micro-crown bud formation was increased as more over 5 mg/L BA concentration, when this time, however, shoot had thick leaves and short internodes, and then withered before long, Micro-crown bud was formed about 88.0% on medium supplemented with 5% sucrose, that was more increased 28% than with 3% sucrose. The buds of crown bud between harvested in field and formed in vitro were difference only in size, but both were similar in shape according to histological view.

• Journal of Plant Biotechnology
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• v.46 no.4
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• pp.303-309
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• 2019

A protocol for the in vitro propagation of Chamaecyparis obtusa was established in the present study. Multi-shoots were initiated from apical shoot explants from germinants after 10 weeks of culture on Litvay medium (LM) supplemented with different concentrations of cytokinin. The effects of pre-treatment with high concentrations of cytokinin and varying concentrations (0.2 to 5.0 mg/L) of zeatin on in vitro shoot elongation and shoot multiplication were investigated. Optimal shoot growth was achieved on LM medium, with over 10-mm shoots after 10 weeks of culture. In the anti-browning tests, ethanesulfonic acid triggered the least browning in the shoot tips. The highest multi-shoot induction was observed in the 0.5-mg/L zeatin treatments, which yielded 80% induction of shoots after 10 weeks of culture, and maximum shoot elongation was observed in the LM basal medium without the hormone. The highest rooting rates were 65% under 0.2 mg/L indole-3-butyric acid.