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http://dx.doi.org/10.3746/jkfn.2016.45.5.664

Effects of Poly-Gamma Glutamate Contents Cheonggukjang on Osteoblast Differentiation  

Lee, Ki Ho (Traditional Korean Medicine Research Team, National Development Institute of Korean Medicine)
Sim, Mi-Ok (Traditional Korean Medicine Research Team, National Development Institute of Korean Medicine)
Song, Yong Su (Heanam Natural Farming Association Cooperation)
Jung, Ho Kyung (Traditional Korean Medicine Research Team, National Development Institute of Korean Medicine)
Jang, Ji-Hun (Traditional Korean Medicine Research Team, National Development Institute of Korean Medicine)
Kim, Min-Suk (Traditional Korean Medicine Research Team, National Development Institute of Korean Medicine)
Kim, Tae Mook (Traditional Korean Medicine Research Team, National Development Institute of Korean Medicine)
Lee, Hyo Eun (Traditional Korean Medicine Research Team, National Development Institute of Korean Medicine)
An, Byeong-Kwan (Traditional Korean Medicine Research Team, National Development Institute of Korean Medicine)
Jung, Won Seok (Traditional Korean Medicine Research Team, National Development Institute of Korean Medicine)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.45, no.5, 2016 , pp. 664-670 More about this Journal
Abstract
Cheonggukjang (CKJ) is a Korean traditional food made of fermented soybeans. In comparison to normal intake of soybeans, Cheonggukjang has high digestibility with bioactive, antioxidant substances, and thrombolytic enzymes. Recent studies have reported anti-oxidant, anti-cancer, anti-inflammatory, anti-obesity activities as well as inhibitory activities against osteoporosis for CKJ. In this study, we identified the effects of CKJ on osteoblast differentiation by increasing the polyglutamic acid (PGA) content of CKJ. Alkaline phosphatase (ALP) activity and mineralization significantly increased in response to treatment with both natural CKJ (CKJ A) and PGA-increased CKJ (CKJ B). However, CKJ B exhibited higher ALP activity and mineralization than CKJ A. Real-time reverse transcription PCR demonstrated that mRNA expression of osteoblastic-associated genes such as type I collagen, alkaline phosphatase, osteocalcin, and osteopontin in C2C12 cells was significantly up-regulated by CKJ A or B treatment. These results indicate that treatment with CKJ has an anabolic effect on bone by increasing osteoblastic differentiation and ALP activity. Increasing PGA content in CKJ had a greater effect than CKJ A on up-regulation of osteoblastic gene expression in osteoblast cells.
Keywords
ALP; Cheonggukjang; osteoblast; osteocalcin; osteopontin;
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