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http://dx.doi.org/10.5010/JPB.2019.46.4.303

In vitro plantlets regeneration by multi-shoots induction and rooting in Chamaecyparis obtusa  

Kim, Ji Ah (Division of Forest Biotechnology, National Institute of Forest Science)
Lee, Na-Nyum (Division of Forest Biotechnology, National Institute of Forest Science)
Kim, Yong Wook (Division of Forest Biotechnology, National Institute of Forest Science)
Publication Information
Journal of Plant Biotechnology / v.46, no.4, 2019 , pp. 303-309 More about this Journal
Abstract
A protocol for the in vitro propagation of Chamaecyparis obtusa was established in the present study. Multi-shoots were initiated from apical shoot explants from germinants after 10 weeks of culture on Litvay medium (LM) supplemented with different concentrations of cytokinin. The effects of pre-treatment with high concentrations of cytokinin and varying concentrations (0.2 to 5.0 mg/L) of zeatin on in vitro shoot elongation and shoot multiplication were investigated. Optimal shoot growth was achieved on LM medium, with over 10-mm shoots after 10 weeks of culture. In the anti-browning tests, ethanesulfonic acid triggered the least browning in the shoot tips. The highest multi-shoot induction was observed in the 0.5-mg/L zeatin treatments, which yielded 80% induction of shoots after 10 weeks of culture, and maximum shoot elongation was observed in the LM basal medium without the hormone. The highest rooting rates were 65% under 0.2 mg/L indole-3-butyric acid.
Keywords
Anti-browning; Chamaecyparis obtuse; Multi-shoot induction;
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Times Cited By KSCI : 1  (Citation Analysis)
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