• Journal of Life Science
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• v.31 no.4
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• pp.385-388
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• 2021

Anther cultivation for crop breeding is a method of rapid production of homozygosities by greatly reducing the time required for at least six generations to develop new varieties using conventional breeding methods. This technique of producing anther culture provides an opportunity to obtain more green plants from a methodological point of view, and the techniques that save time and effort in anther culture are also important because they increase the efficiency of culture. This study compared the callus induction rate and green plant regeneration rate of a one-step and a two-step culture that differ in their culture media and culture methods. One-step culture allows callus induction and plant regeneration in one medium, whereas two-step culture requires induction and plant regeneration in two different media. In this study, we compared the callus induction and plant regeneration rates of rice anthers as one-step and two-step cultures. The callus formation rate was 13.0% for one-step cultures and 8.6% for two-step cultures, so the rate was 4.4% higher for one-step cultures than for two-step cultures. The plant regeneration rate was 1.0% in one-step cultures and 3.0% in two-step cultures, so the regeneration rate was three times higher for the two-step cultures than for one-step cultures. This suggests that the two-step cultures are more efficient than the one-step cultures for haploid production.

• Animal Systematics, Evolution and Diversity
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• v.13 no.1
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• pp.21-27
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• 1997

한국 고유종인 새코미꾸리(Cobitis rotundicaudata)의 집단간 유전적 차이에 따른 종 분화 여부를 밝히고자 4개집단을 대상으로mitochondrial DNA(mtDNA)의 RFLP분석을 실시 하였다. C. rotundicaudata mtDNA를 10개의 6-base cutting 제한요소로 처리한 다음 그 절 편 양상을 비교, 분석한 결과 4개 집단 공히 mtDNA의 전체 genome 크기는 약 16.5$\pm$ 0.5Kbp였으며 공통절편수(F)에서 한강 2개 집단(가평, 진부)과 동해안 마읍천 짐단간의 F값 은 0.911로 매우 가까웠으나 낙동강의 산청집단은 타 3개 집단과 F=0.375로 차이가 있었다. 또한 염기치환율(p)에 있어서도 한강 2개 집단 및 마읍천 집단간은 평균 p=0.005로 매우 유 사하였으나, 산청 집단은 타 집단들과 염기치환율에 있어 p=0.059로 종수준의 뚜렷한 차이 를 나타내어서 이들은 각각 별종으로 사료된다.

• Korean Journal of Plant Tissue Culture
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• v.27 no.6
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• pp.479-484
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• 2000

This study was conducted to examine the effects of explant sources and plant growth regulators on mass propagation of Cyclamen persicum. Tuber, cotyledon, and petiole tissues were cultured on MS medium supplemented with different concentrations and combinations of auxins and cytokinins. Shoots were not induced from calli on cotyledon and petiole explants cultured on MS medium containing various concentrations of 2,4-D or NAA. However, multiple shoots were formed directly from tuber explants cultured on the medium containing 0.5 and 1.0 mg/L 2,4-D or NAA. In MS medium with cytokinin alone, TDZ was more effective in shoot formation than BA or kinetin in all explants. The combinations of NAA and BA was found to be most effective in shoot formation from tuber, cotyledon and petiole explants. Especially, shoots were formed from all the tuber explants on the medium containing 0.5 mg/L of NAA and BA. Hormonal effects on root formation were examined by subculturing single shoots on MS medium containing NAA or IBA. The medium with 0.5 mg/L IBA was most effective in root induction and subsequent plantlet regeneration.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.1
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• pp.29-36
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• 2004

In an effort to optimize tissue culture responses of zoysiagrass(Zoysia japonica Steud.) for genetic transformation, factors affecting callus induction and plant regeneration were investigated. MS medium containing 3 mg/L 2,4-D was optimal for embryogenic callus induction from mature seed. The plant regeneration frequency of 73.3% was observed when embryogenic calli induced in this medium were transferred to N6 medium supplemented with 0.1 mg/L 2,4-D and 5 mg/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Regenerated plants were grown normally when shoots transplanted to the soil. A rapid and efficient plant regeneration system established in this study will be useful for molecular breeding of turfgrass through genetic transformation.

• Journal of Animal Science and Technology
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• v.47 no.6
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• pp.1067-1074
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• 2005

In order to optimize tissue culture conditions of Kentucky bluegrass(Poa pratensis L.), effects of culture medium supplements, media and cultivars on embryogenic callus induction and regeneration of plants were investigated. MS medium containing 3mg/L 2,4-D and 0.1mg/L BA was optimal for embryogenic callus induction from mature seeds. The highest plant regeneration frequency(57.7%) was observed when the embryogenic calli were cultured on N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Genotype was an important factor in plant regenerability. ‘Newport’ showed to have higher regeneration frequency of 53.4%. Regenerated plants were grown normally when shoots transplanted to the soil. A short tissue culture period and high-frequency regeneration system would be beneficial for molecular breeding of Kentucky bluegrass through genetic transformation.

• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.49-55
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• 2017

We investigated the callus induction and plant regeneration ability of 16 maize genotypes, including the Korean inbred lines, using 9 to 15 day-old immature zygotic embryos from maize grown in pots and from field cultures. Immature zygotic embryos placed on MS medium supplemented with L-proline 0.7 g/L, MES 0.5 g/L, Dicamba 1.5 mg/L, 2,4-D 0.5 mg/L, $AgNO_3$ 4 mg/L, and sucrose 20 g/L, showed the highest frequency of callus induction. The highest number of shoots regenerated when the embryogenic callus were transferred to MS medium supplemented with 5 mg/L zeatin. The root formation was observed when shoots were grown on MS medium supplemented with 0.2 mg/L indole-3-butyric acid (IBA). Additionally, under the same culture conditions, immature zygotic embryos from maize grown in the field also had a high frequency of plant regeneration. Except one genotype, 15 genotypes showed callus induction and shoot regeneration. Among the 16 genotypes tested, H99, B98, HW3, and B73 yielded the best plant regeneration. H99 showed maximum shoot formation from the primary embryogenic callus. The results suggest that genotypes and growth conditions of the maize plant plays very important roles for enhancing the embryogenesis competence of immature zygotic embryos. The successful regeneration from immature zygotic embryos of maize inbred lines provides a basis for molecular breeding of new cultivars by genetic transformation.

• Horticultural Science & Technology
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• v.31 no.6
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• pp.726-731
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• 2013

Experiments were conducted to evaluate the effect of low temperature-darkness treatment on floral initiation in four kinds of Korean strawberry cultivars. Mother plants were planted on March 29 and daughter plants were raised for the experiment. Temperature treatment was done for 7, 14, and 21 days keeping $13^{\circ}C$ in cooling storage from August 29, September 5 and September 14 to September 21. After the treatment, flower bud initiation was examined by a microscope, and the seedlings were transplanted on hydroponic system with Yamazaki's strawberry solution of EC $0.8dS{\cdot}m^{-1}$ to check the flowering. 'Ssanta' and 'Maehyang' showed early flower bud initiation in 7 days treatment than 'Seolhyang' by microscope check. 'Ssanta', 'Daewang', and 'Maehyang' showed early flower bud initiation in 14 days treatment than 'Seolhyang'. There were no differences among the treatments in 21 days treatment. Percentage of flowering of 'Seolhyang' and 'Maehyang' by low temperature-darkness treatment didn't show any difference but 'Daewang' and 'Ssanta' showed high percentage of flowering. It suggested that low temperature-darkness treatment technique can be use for improving early flowering and yield of 'Ssanta' and 'Daewang' cultivars.

• Proceedings of the Korean Society of Grassland Science Conference
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• 2000.09a
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• pp.100.1-100
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• 2000

• Korean Journal of Plant Resources
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• v.16 no.1
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• pp.34-39
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• 2003

Corm apical meristem cultures of thirteen glasshouse freesia cultivars were tested to investigate the possibility of micropropagation using MS basal medium supplemented with 2,4-D, NAA(0.1, 0.5, 1.0mg/L, respectively) and BA (0.5∼2.0mg/L). The majority of the tested cultivars could be induced callus and shoot buds in all culture condition. The combinations of NAA and BA appeared superior to that of 2,4-D and BA depending on cultivars for callus induction and shoot formation. Among the cultivars, 'Golden Yellow' showed the highest regeneration capacity on MS media with 0.5mg/L NAA and 1.0 mg/L BA. The highest percentage of regeneration and the greatest number of shoot from calli were obtained through successive subculture on MS medium supplimented with 0.5mg/L BA. In that condition, more than 60 ％ shoot regeneration and average of 25.1 shoots per explant was achieved. Transformed shoots on half-strength MS medium without plant growth regulators rooted easily.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.4
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• pp.297-302
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• 1999

Cotyledonary abnormalities were observed in secondary somatic embryos which were developed from calli cultured on MS medium with various concentrations of 2,4-D. In MS medium containing hormone-free or $0.1mg/{\ell}$ 2,4-D, the frequency of normal embryo formation with two cotyledons were above 57%. According to concentration of 2,4-D increment the frequency of normal embryos were decreased. In MS medium containing $4mg/{\ell}$ 2,4-D, the frequency of normal embryo formation was just 10%. The rate of germination was as follows; 37.7% of somatic embryos had one cotyledon, 85% two cotyledons, 38% three cotyledons, 35% four cotyledons, 25% five cotyledons and 29% trumpet-like cotyledons. About 80% of the embryos with two cotyledons were converted into normal plants, but one, three or four cotyledons were only 6.8~10%. The five or trumpet-like embryos were not developed into normal plants.