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http://dx.doi.org/10.5333/KGFS.2004.24.1.029

Factors Affecting Callus Induction and Plant Regeneration from Mature Seed of Zoysiagrass (Zoysia japonica Steud.)  

이상훈 (경상대학교 응용생명과학부, 농업생명과학연구원)
김범수 (경상대학교 응용생명과학부, 농업생명과학연구원)
원성혜 (경북대학교 농업생명과학대학)
조진기 (경북대학교 농업생명과학대학)
김기용 (축산기술연구소)
박근제 (축산기술연구소)
성병렬 (축산기술연구소)
이효신 (국립산림과학원)
이병현 (경상대학교 응용생명과학부, 농업생명과학연구원)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.24, no.1, 2004 , pp. 29-36 More about this Journal
Abstract
In an effort to optimize tissue culture responses of zoysiagrass(Zoysia japonica Steud.) for genetic transformation, factors affecting callus induction and plant regeneration were investigated. MS medium containing 3 mg/L 2,4-D was optimal for embryogenic callus induction from mature seed. The plant regeneration frequency of 73.3% was observed when embryogenic calli induced in this medium were transferred to N6 medium supplemented with 0.1 mg/L 2,4-D and 5 mg/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Regenerated plants were grown normally when shoots transplanted to the soil. A rapid and efficient plant regeneration system established in this study will be useful for molecular breeding of turfgrass through genetic transformation.
Keywords
Callus; Plant regeneration; Turfgrass; Zoysia japonica;
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Times Cited By KSCI : 1  (Citation Analysis)
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