• Journal of Korean Society of Forest Science
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• v.78 no.4
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• pp.412-418
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• 1989

The stem segments of Ailanthus altissima were cultured on the Murashige & Skoog's medium(1962) supplemented with 0.1 mg/l BAP and 1.0 mg/l 2, 4-D for callus induction and proliferation, Shoot primordia were observed as greenish regions on the surface of yellow-brown calli about 8 weeks after culture. Shoot primordia were selected and transferred to the MS media containing various combination of BAP and 2, 4-D. Among these combinations the shoot primordia cell clusters on the medium added to 0.5mg/l BAP and 0.01mg/l 2, 4-D exhibited the highest number of shoot formation. These shoots were successfully transferred on the solid MS medium with no growth regulators for the rootings.

• Korean Journal of Agricultural Science
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• v.20 no.2
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• pp.133-144
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• 1993

Calli were induced on microtuber disks of potato(S.tuberosum) infected with three binary vectors transconjugated with C58, A281 and LBA 4404 of Agrobacterium tumefaciens and pBI121. The frequency inducing callus was the highest by infection of C121 carrying pC58 and pBI121, and shoots were differentiated on the calli without any hormonal application. Transformed calli were selected by their resistance to kanamycin and identified by GUS activity. The frequency of callus formation by infection of binary vector strain was affected according to the hormonal application.

• Journal of Animal Science and Technology
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• v.46 no.6
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• pp.967-974
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• 2004

The current study was undertaken to determine the effect of various conjugated linoleic acid (CLA) isomers on differentiation of pig preadipocyte during culture. Preadipocyte(stroma-vascular cell) was isolated from the backfat of newborn pigs and cultured to differentiate into mature fat cell. Different doses of CLA isomers were treated to the culture media at different times. Cell differentiation was determined by measuring the glycerol3-phosphate dehydrogenase activity of the cultured preadipocytes. Twenty and fifty $\mu$M of trans110_cis 12 isomer of CLA inhibited differentiation of pig preadipocyte whereas cis9-cis II isomer stimulated the differentiation. Both cis9-transII and trans9-trans11 isomers showed no effect. Effect of CLA isomer was more evident at the early stage of culture(day 0-8), than the late stage(day 8-14). These results suggest that each CLA isomer has different effect on pig preadipocyte differentiation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.2
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• pp.116-122
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• 1982

In three diverse wheat and one barley cultivars were used to examine the relation of the spikelet and floret differentiation to fertility and an winter wheat Norin 61 was used to examine the spikelet size gradients in the mature ear in relation to their grain development. The greatest number of grains and florets per spikelet, and the heaviest grains occurred in the low-mid part of the ear. Within the spikelets, the 2nd grain from the base was heavier than the I st or the 3rd one. In this region, the fertility of the 1 st floret was 97%, the 2nd 93%, the 3rd 88%, and 4th floret was 15%. The fertility of the wheat and barley was closely related to spikelet and floret differentiation. Barley spikelets was fertile as their differentiation stage was X that is 25 days before heading. Wheat florets were fertile as their differentiation stage was X that is 15-20 days before heading. But, they were sterile or reduced in fertility if they were not reached to the stage X.

• Korean Journal of Plant Tissue Culture
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• v.22 no.4
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• pp.207-211
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• 1995

Immature zygotic embryos from immature seeds of Wasabia japonica (cv Dalma) were isolated and cultured on modified MS medium supplemented with 2,4-D, IAA, and BA. Immature zygotic embryos were classified into torpedo shape and cotyledon stage. The highest rates of callus formation were obtained of 1.0mg/L IAA(torpedo stage, 90.0%)and 1.0mg/L 2,4D plus 0.1mg/L BA(cotyledany stage,84.3%). Somatic embryos after 60 days of culture. These numerous somatic embryo could be seperated and subcultured on the same media for further propagation. After 90 days of culture, most somatic embryos were developed well organized embryos which were able to produce into whole plants.

• Korean Journal of Plant Resources
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• v.24 no.5
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• pp.628-635
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• 2011

This study was conducted to establish the tissue culture system for Korean domestic Miscanthus sinensis, which is used in various purposes such as forage, and bio-energy resources. With the mature seed of Miscanthus, optimum concentrations of plant growth regulators were identified for an efficient callus induction and regeneration. Among the treatments of 1~10 $mg{\cdot}L^{-1}$ 2,4-D, IBA, or NAA, callus induction rate was highest (85.3%) on MS medium containing 5 $mg{\cdot}L^{-1}$ 2,4-D. Under the condition, the callus were efficiently induced and proliferated with comparably lower frequencies of callus browning. In shoot regeneration, the treatment of NAA combined with BAP seemed to contribute more efficient conditions to shoot regeneration than those of NAA with Kinetin or 2-iP. Especially, regeneration efficiency and number of regenerated plants were 83.7% and 5.5 in 3 $mg{\cdot}L^{-1}$ NAA with 5 $mg{\cdot}L^{-1}$ BAP, respectively, which were higher frequencies than those in NAA with Kinetin or 2-iP. In results, 5 $mg{\cdot}L^{-1}$ 2,4-D and 3 $mg{\cdot}L^{-1}$ NAA combined with 5 $mg{\cdot}L^{-1}$ BAP were efficient for embryogenic callus induction and regeneration of Miscanthus. This system would be useful for mass-propagation and developing new cultivars via tissue culture of Miscanthus sinensis.

• The Science & Technology
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• v.35 no.2 s.393
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• pp.10-13
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• 2002

2001년의 가장 괄목할 과학 연구업적/초기 인간배아 복제 성공/새로운 디자인의 엔진/인간 줄기세포를 뇌세포로 전환/DNA 컴퓨터 개발/태양계 밖 행성의 대기상태 밝혀내/세계에서 가장 빠른 레이저/화성에 한때는 물이 지구보다 많았다/눈 무게 때문에 지구가 축소 팽창한다/목성의 위성 이오의 화산 분화구 촬영/산소원자 4개로 이뤄진 산소분자 발견/침팬지 지놈 98.7%가 인간과 동일/바다거북의 항해 기술

• Journal of The Korean Society of Grassland and Forage Science
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• v.20 no.1
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• pp.25-30
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• 2000

The conditions for callus formation and plant regeneration were confirmed in Italian ryegrass (Lolium mulfiorum Lam.). Among SH (Schenk and Hildebrandt), MS (Murashige and Skoog) and N6 medium (Chu) MS medium was highest degree of efficiencies respectively in callus formation and plant regeneration. In this study, we determined volume of hormones and other compounds appended in media. For callus formation, only $5\;mg/\;{\ell}$ of 2,4-D (2,4-dichlorophenoxy acetic acid) was appended in their media. For plant regeneration, we used MS medium containing $1.0\;mg/\;{\ell}$ of BA and $0.1\;mg/\;{\ell}$ of NAA.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.87-87
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• 2019

현대인의 생활수준의 향상에 따른 관상식물의 관심이 증가하여, 자생초화류를 활용하기 위한 연구가 진행되고 있다. 산꼬리풀[Veronica rotunda var. subintegra (Nakai) T.Yamaz.]은 국내 특산식물로서 산지의 초원에서 자생하고, 쪽빛을 내는 화기는 청아한 느낌을 주어 관화식물로서 이용가능성이 높다. 본 연구는 산꼬리풀의 분화 재배기술을 확립하고자 2018년 7월 2일부터 10월 15일까지 수행하였다. 대조구는 플라스틱 화분 10호에 원예상토를 충진한 다음 200구에 셀당 4립 파종하여 생산된 유묘를 정식하였다. 실험은 식재용기(플라스틱 8, 10, 12호 화분, 비닐포트 3, 4치), 토양종류[원예상토 단용, 중화 피트모스:펄라이트(3:1, 4:1), 마사토:중화 피트모스(2:1, 3:1)], 묘의 소질[파종립수(1, 2, 4, 6립), 파종용기(162, 200, 288구 트레이)] 등이 생육에 미치는 영향을 분석하였다. 연구의 결과, 식재용기에 따른 생육은 플라스틱 화분 12호에서 왕성하였고, 포트의 용적량이 클수록 경직경, 측지수, 마디수, 엽수, 개화율이 유의적으로 증가하였다. 토양종류에 따른 생육은 원예상토에서 가장 왕성하였고, 측지수, 경직경, 마디수, 엽수 및 개화율이 모두 우수하여 관상용 재배에 적합하였다. 피트모스 혼용토는 생육이 억제되는 동시에 잎이 황화되는 현상이 나타났다. 마사토 혼용토의 경우, 생육이 억제되는 결과를 보였다. 셀당 파종량을 달리한 유묘를 정식하여 재배한 결과, 초장, 절간장은 파종량이 증가할수록 감소하였고, 포트당 마디수와 엽수는 증가하였으나, 1립 파종묘를 제외하고 유의적인 차이는 없었다. 개화율은 4립 파종묘(44.4%)가 1, 2, 6립 파종묘(20.8~25.0%)보다 높았다. 육묘용기별 생육은 초기생육이 우수하였던 162구 생산묘가 정식 후 재배에도 왕성한 생육을 나타내었다. 결론적으로, 산꼬리풀의 최적의 분화재배를 위해 플라스틱 화분 12호에 원예상토를 충진한 다음 162구 트레이에 포트당 엽수와 개화율이 높은 4립 파종하여 생산된 유묘를 정식하여 재배하는 것이 우수할 것으로 판단된다. 경제적 측면을 고려하여 토양은 비교적 생육이 양호하였던 마사토의 활용방안을 검토하는 것이 좋을 것으로 생각된다.

• Journal of fish pathology
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• v.9 no.1
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• pp.95-109
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• 1996

This work was carried out to investigate the functional heterogeneity of peripheral blood lymphocytes(PBLs) in carp, Cyprinus carpio. PHA, Con A, LPS and BCG were injected intraperitoneally into carp to determine the blastogenic response and rosette formation activity. In each group of fish treated with stimulators, the cell numbers and DNA contents of lymphocytes were higher than those of untreated control group and reached the highest level between 1 week and 2 weeks after injection with mitogens. These results showed that BCG and Con A were strong stimulators of proliferation compared to PHA and LPS. However, PHA-treated fish twice showed the highest rosette formation response among the consecutive stimulations with the same mitogen. Alase, the results on consecutive mitogen stimulation revealed that carps reinjected by different mitogens led to an increased stimulation higher than the one reinjected after 1 week with same mitogen. It seems that different mitogens may stimulate different cell populations and implies functionally separated subpopulations of lymphocytes in carp.