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http://dx.doi.org/10.5187/JAST.2004.46.6.967

Effect of Isomers of Conjugated Linoleic Acid on Porcine Preadipocyte Differentiation  

Moon, H.S (Department of Animal Science, Chungbuk National University)
Chung, C.S. (Department of Animal Science, Chungbuk National University)
Publication Information
Journal of Animal Science and Technology / v.46, no.6, 2004 , pp. 967-974 More about this Journal
Abstract
The current study was undertaken to determine the effect of various conjugated linoleic acid (CLA) isomers on differentiation of pig preadipocyte during culture. Preadipocyte(stroma-vascular cell) was isolated from the backfat of newborn pigs and cultured to differentiate into mature fat cell. Different doses of CLA isomers were treated to the culture media at different times. Cell differentiation was determined by measuring the glycerol3-phosphate dehydrogenase activity of the cultured preadipocytes. Twenty and fifty $\mu$M of trans110_cis 12 isomer of CLA inhibited differentiation of pig preadipocyte whereas cis9-cis II isomer stimulated the differentiation. Both cis9-transII and trans9-trans11 isomers showed no effect. Effect of CLA isomer was more evident at the early stage of culture(day 0-8), than the late stage(day 8-14). These results suggest that each CLA isomer has different effect on pig preadipocyte differentiation.
Keywords
CLA; Pig; Preadipocyte; Differentiation;
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1 Ding, S-T., McNell, R. L. and Mersmann, H. J. 2002. Conjugated linoleic acid increases the differentiation of porcine adipocytes in vitro. Nutrition Research 20:1569-1580.   DOI   ScienceOn
2 Ding, S. T., Wang, J. C. and Mersmann, H. J. 2003. Effect of unsaturated fatty acid on porcine adipocyte differentiation. Nutrition Research 23:1059-1069.   DOI   ScienceOn
3 Dugan, M. E. R., Aalhus, K. L., Schaefer, A. L. and Kramer, J. K. G. 1997. The effects of linoleic acid on fat to lean repartitioning and feed con-version in pigs. Can. J. Anim. Sci. 77:723-725.
4 Ha, Y. L., Grimm, N. K and Pariza, M. W. 1987. Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. Carcinogenesis. 8:1881-1887.
5 Ip, C, Scimerca, J. A. and Thompson, H. J. 1994. Conjugted linoeic acid, a powerful anticarcinogen from animal fat sources. Cancer 74:1050-1054.   DOI   ScienceOn
6 Thiel-Cooper, R. L., Parrish, F. c., Sparks, J. C., Wiegand, B. R. and Ewan, R. C. 2001. Conjugated linoleic acid changes swine performance and carcass composition. J. Anim. Sci. 79:1821-1828.   DOI
7 Wahle, K. W. J., Heys, S. D. and Rotondo, D. 2004. Conjugated linoleic acids: are they beneficial or detrimental to health? Progress in Lipd Reaearch. 43:553-587.
8 Waylan, A. T., O'Quinn, P. R., Unruh, J. A., Nelssen, J. L., Goodband, R. D., Woodworth, J. C, Tokach, M. D. and Koo, S. I. 2002. Effects of modified tall oil and vitamin E on growth performance, carcass characteristics, and meat quality of growing-finishing pigs. J. Anim. Sci. 80:1575-1585.   DOI
9 Brodie, A. E., Manning, V. A., Ferguson, K. R., Jewell, D. E. and Hu, C. Y. 1999. Conjugated linoleic acid inhibits differentiation of pre- and postconfluent 3T3-L1 preadipocytes but inhibits cell proliferation only in preconfluent cell. J. Nutr. 129:602-606   DOI
10 Wise, L. S. and Green, H. 1979. Participation of one isozyme of cytosolic glycerophosphate in adipose conversion of 3T3 cells J. Biol. Chem. 254:273-275.
11 Bradford, M. M. 1976. A rapid and sensitive method for the quantiation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72:248-254.   DOI   ScienceOn
12 Cook, M. R., Miller, C. C., Park, Y. and Paliza, M. W. 1993. Immune modulation by altered nutrient metabolism-nutritional control of immune-induced growth depression. Poult Sci. 72:1301-1305.   DOI   ScienceOn
13 Ostrowska, E., Mualitharan, M, Cross, R. F., Bauman, D. E. and Dunshea, F. R. 1999. Dietary conjugated linoleic acids increase lean tissue and decrease fat deposition in growing pigs. J. Nutr. 129:2037-2042.   DOI
14 Kang, K, Liu, W., Albright, K. J., Park, Y. and Pariza, M. W. 2003. trans-10, cis-12 CLA inhibits differentiation of 3T3-L1 adipocytes and decreases PPAR$\gamma$ expression. BBRC 303:795-799.
15 Lee, K. N., Kritchevsky, D. and Pariza, M. W. 1994. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108:19-25.   DOI   ScienceOn
16 McNeil, R. L. and Mersmann, H. J. 2003. Effects of isomers of conjugated linoleic acid on porcine adipocyte growth and differentiation. J. Nutr. Biochem. 14:266-274.   DOI   ScienceOn
17 SAS. 1998. SAS User's Guide: Statistics, SAS Inst, Inc., Cary, N.C.
18 Satory, D. and Smith, S. B. 1999. Conjugated linoleic acid inhibits proliferation but stimulates lipid filling of murine 3T3-L1 preadipocytes. J. Nutr. 129:92-97.   DOI
19 Suryawan, A., Swanson, L. V. and Hu, C. Y. 1997. Insulin and hydrocortisone, but not triiodothyronine, are required for the differentiation of pig preadipocytes in primary culture. J. Anim. Sci. 75:105-111.   DOI