Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Effect of Isomers of Conjugated Linoleic Acid on Porcine Preadipocyte Differentiation

Conjugated Linoleic Acid(CLA) 이성체가 돼지 지방전구세포의 분화에 미치는 영향

  • Moon, H.S (Department of Animal Science, Chungbuk National University) ;
  • Chung, C.S. (Department of Animal Science, Chungbuk National University)
  • 문현석 (충북대학교 농과대학 축산학과) ;
  • 정정수 (충북대학교 농과대학 축산학과)
  • Published : 2004.12.31
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Abstract

The current study was undertaken to determine the effect of various conjugated linoleic acid (CLA) isomers on differentiation of pig preadipocyte during culture. Preadipocyte(stroma-vascular cell) was isolated from the backfat of newborn pigs and cultured to differentiate into mature fat cell. Different doses of CLA isomers were treated to the culture media at different times. Cell differentiation was determined by measuring the glycerol3-phosphate dehydrogenase activity of the cultured preadipocytes. Twenty and fifty $\mu$M of trans110_cis 12 isomer of CLA inhibited differentiation of pig preadipocyte whereas cis9-cis II isomer stimulated the differentiation. Both cis9-transII and trans9-trans11 isomers showed no effect. Effect of CLA isomer was more evident at the early stage of culture(day 0-8), than the late stage(day 8-14). These results suggest that each CLA isomer has different effect on pig preadipocyte differentiation.

본 연구는 여 러 conjugated linoleic acid(CLA) 이성체가 돼지 지방전구세포의 분화에 미치는 영향을 구영하기 위해 수행하였다. 돼지 지방전구세포는 갓난 돼지의 등 지방에서 분리해서 성숙지방세포로 분화될 때 까지 배양했다. 여러 CLA 이성체를 배양중의 세포에 처리했다. 세포분화는 세포배양이 끝난 후 세포의 glycerol-3-phosphate의 활성도를 측정함으로써 구명했다. 20$\mu$M과 50$\mu$M의 transto-cis12 CLA 이성체는 돼지 지방천구세포의 분화를 억제했고，한펀 cis9-cisl1 이성체는 세포분화를 촉진했다. cis9-trans 11 과 trans9-trans11 이생체는 세포분화에 아무런 영향을 마치지 않았다. CLA의 세포분화에 미치는 작용은 배양후기 (day8${\sim}$14) 보다 배양전기(day 0${\sim}$ 8)에 더 두드러지게 나타냈다. 위의 결과는 여러 CLA 이성체는 돼지 지방천구세포 분화에 각각 다른 작용을 가짐을 나타낸다.

Keywords

References

  1. Brodie, A. E., Manning, V. A., Ferguson, K. R., Jewell, D. E. and Hu, C. Y. 1999. Conjugated linoleic acid inhibits differentiation of pre- and postconfluent 3T3-L1 preadipocytes but inhibits cell proliferation only in preconfluent cell. J. Nutr. 129:602-606 https://doi.org/10.1093/jn/129.3.602
  2. Bradford, M. M. 1976. A rapid and sensitive method for the quantiation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72:248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  3. Cook, M. R., Miller, C. C., Park, Y. and Paliza, M. W. 1993. Immune modulation by altered nutrient metabolism-nutritional control of immune-induced growth depression. Poult Sci. 72:1301-1305. https://doi.org/10.3382/ps.0721301
  4. Ding, S-T., McNell, R. L. and Mersmann, H. J. 2002. Conjugated linoleic acid increases the differentiation of porcine adipocytes in vitro. Nutrition Research 20:1569-1580. https://doi.org/10.1016/S0271-5317(00)00241-4
  5. Ding, S. T., Wang, J. C. and Mersmann, H. J. 2003. Effect of unsaturated fatty acid on porcine adipocyte differentiation. Nutrition Research 23:1059-1069. https://doi.org/10.1016/S0271-5317(03)00081-2
  6. Dugan, M. E. R., Aalhus, K. L., Schaefer, A. L. and Kramer, J. K. G. 1997. The effects of linoleic acid on fat to lean repartitioning and feed con-version in pigs. Can. J. Anim. Sci. 77:723-725.
  7. Ha, Y. L., Grimm, N. K and Pariza, M. W. 1987. Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. Carcinogenesis. 8:1881-1887.
  8. Ip, C, Scimerca, J. A. and Thompson, H. J. 1994. Conjugted linoeic acid, a powerful anticarcinogen from animal fat sources. Cancer 74:1050-1054. https://doi.org/10.1002/1097-0142(19940801)74:3+<1050::AID-CNCR2820741512>3.0.CO;2-I
  9. Kang, K, Liu, W., Albright, K. J., Park, Y. and Pariza, M. W. 2003. trans-10, cis-12 CLA inhibits differentiation of 3T3-L1 adipocytes and decreases PPAR$\gamma$ expression. BBRC 303:795-799.
  10. Lee, K. N., Kritchevsky, D. and Pariza, M. W. 1994. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108:19-25. https://doi.org/10.1016/0021-9150(94)90034-5
  11. McNeil, R. L. and Mersmann, H. J. 2003. Effects of isomers of conjugated linoleic acid on porcine adipocyte growth and differentiation. J. Nutr. Biochem. 14:266-274. https://doi.org/10.1016/S0955-2863(03)00031-7
  12. Ostrowska, E., Mualitharan, M, Cross, R. F., Bauman, D. E. and Dunshea, F. R. 1999. Dietary conjugated linoleic acids increase lean tissue and decrease fat deposition in growing pigs. J. Nutr. 129:2037-2042. https://doi.org/10.1093/jn/129.11.2037
  13. SAS. 1998. SAS User's Guide: Statistics, SAS Inst, Inc., Cary, N.C.
  14. Satory, D. and Smith, S. B. 1999. Conjugated linoleic acid inhibits proliferation but stimulates lipid filling of murine 3T3-L1 preadipocytes. J. Nutr. 129:92-97. https://doi.org/10.1093/jn/129.1.92
  15. Suryawan, A., Swanson, L. V. and Hu, C. Y. 1997. Insulin and hydrocortisone, but not triiodothyronine, are required for the differentiation of pig preadipocytes in primary culture. J. Anim. Sci. 75:105-111. https://doi.org/10.2527/1997.751105x
  16. Thiel-Cooper, R. L., Parrish, F. c., Sparks, J. C., Wiegand, B. R. and Ewan, R. C. 2001. Conjugated linoleic acid changes swine performance and carcass composition. J. Anim. Sci. 79:1821-1828. https://doi.org/10.2527/2001.7971821x
  17. Wahle, K. W. J., Heys, S. D. and Rotondo, D. 2004. Conjugated linoleic acids: are they beneficial or detrimental to health? Progress in Lipd Reaearch. 43:553-587.
  18. Waylan, A. T., O'Quinn, P. R., Unruh, J. A., Nelssen, J. L., Goodband, R. D., Woodworth, J. C, Tokach, M. D. and Koo, S. I. 2002. Effects of modified tall oil and vitamin E on growth performance, carcass characteristics, and meat quality of growing-finishing pigs. J. Anim. Sci. 80:1575-1585. https://doi.org/10.2527/2002.8061575x
  19. Wise, L. S. and Green, H. 1979. Participation of one isozyme of cytosolic glycerophosphate in adipose conversion of 3T3 cells J. Biol. Chem. 254:273-275.

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