• The Zoological Society Korea : Newsletter
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• v.18 no.2
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• pp.21-25
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• 2001

대부분의 조직은 여러 가지 세포가 모여서 이루어지기 때문에 그 중의 어떤 특정세포에서 발현하는 물질을 분석하려면 조직을 이루고 있는 각각의 세포를 분리해내야 한다. 이렇게 순수하게 세포를 분리해내는 기술 중의 하나가 Laser Captured Microdissection (LCM)이 다. LCM의 개발로 기존에 사용되던 방법에 비하여 빠르고 간편하면서, 매우 정확하게 원하는 세포를 순수 분리해서 그 세포의 분자생물학적 또는 생화학적인 분석을 할 수 있게 되었다. LCM은 현미경으로 조직절편을 관찰하면서 원하는 세포를 낮은 에너지의 laser를 사용하여 도려내는 방법으로 조직절편 이외에도 도말된 혈액이나 자궁경부 조직, 그리고 배양된 세포를 cytocentrifugation한 후에 원하는 세포를 포획 할 수도 있다. LCM을 이용한 연구는 여러 분야에서 다양하게 진행되고 있으며, 특히 같은 조직 내에 존재하는 정상세포와 전이중인 세포, 그리고 암세포를 구분해 냄으로써 암의 전이기전 및 병인 연구에 매우 큰 공헌을 하고 있다. 이렇게 분리된 세포는 RT-PCR, LOH (loss of heterozygosity), microsatellite instability, differential gene profiling, cDNA microarray, Western blot, 2D PAGE protein analysis 등의 기법을 접목하여 연구하게 된다. 본 논단을 통하여 1996년 개발된 LCM의 원리와 이제까지 LCM을 이용한 연구 성과를 살펴보고자 한다.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.165-165
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• 2016

장기적인 수자원 계획의 수립이나 수공구조물을 설계함에 있어 수문학적 순환은 매우 중요하다. 그러나 수문학적 순환은 인간활동과 기후변화의 요인으로 인하여 시간 및 공간에 따라 항상 변화되기 때문에 특정지점에서의 유량도 수문학적 순환의 변동으로 인하여 변화된다. 따라서 본 연구에서는 특정 지점에서 취득된 과거 유량자료를 이용하여 기후변화와 인간활동에 의해 변화되는 유량을 정량적으로 분리할 수 있는 두 가지 기법을 개발하였다. 인간활동에 의해 변화되는 유량(인간활동 유량변동)에 영향을 미치는 요인으로는 인구 증가, 토지이용 변화 등을 들 수 있고, 기후변화에 의해 변화되는 유량(기후변화 유량변동)에 영향을 미치는 요인으로는 온난화로 인한 강수, 기온, 습도 등의 변화를 들 수 있다. 이러한 요인으로 인한 유량의 변동을 분석하기 위해서는 먼저 관측된 자료가 가지고 있는 변동점을 분석하여 변동점 이전 구간을 비영향구간(non-impacted period), 이후 구간을 영향구간(impacted period) 로 나누어야 하는데 이를 위하여 이중누가곡선, 선형회귀분석, Mann-Kendall 검정, Pettitt 검정을 이용하였다. 인간활동 및 기후변화 유량변동을 정량적으로 분리함에 있어서는 두 가지 분석기법이 사용될 수 있다. 즉 인간활동 유량변동을 먼저 분석한 이후 기후변화 유량변동을 산정하는 기법을 적용할 수도 있고 반대로 기후변화 유량변동을 먼저 산정한 이후 인간활동 유량변동을 산정할 수도 있는데 본 연구에서는 두 가지 기법을 모두 적용하여 그 결과를 비교하였다. 인간활동 유량변동을 먼저 산정하는 기법을 적용하기 위해서 비영향구간에 대해 SWAT-CUP을 활용하여 보정된 SWAT 모형을 구축하여 결과의 산정에 활용하였으며, 기후변화 유량변동을 먼저 산정하는 기법을 적용하기 위해서는 영향구간에 대해 민감도 분석 기법 중의 하나인 Budyko 분석을 활용하였다. 본 연구에서 개발된 두 가지 기법을 이용하여 인간활동 및 기후변화 유량변동을 정량적으로 산정하여 비교함에 있어 한강 유역의 소양강댐 상류유역과 섬강유역을 대상으로 과거 관측자료를 취득하여 연구를 수행하였다. 소양강댐 상류유역과 섬강유역의 변화된 유량에 대해서 기후변화로 인한 영향의 비율이 높은지 인간활동으로 인한 영향의 비율이 높은지를 파악함으로써 궁극적으로 두 유역에서의 인간활동과 기후변화로 인해 발생된 유량의 변화 요인을 정량적으로 분리하여 파악할 수 있었다. 그러므로 이와 같은 연구를 활용하여 수문학적 순환 관계에 있어 앞으로 어떠한 요인이 유량변동에 많은 영향을 미치는지 예측하여 미래에 변화하는 유량에 대해 계획하고 수공구조물을 설계함에 있어 활용될 수 있는 설계지침을 제시할 수 있었다.

• Journal of Sericultural and Entomological Science
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• v.33 no.1
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• pp.27-31
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• 1991

Microsporidian spores were isolated from yellow-hind winged arctiid, Eilema griseola (Hubner), and cutworm, Agrotis tokionis Butler, and their morphological characteristics and their indectivity to silkworms were investigated. The shape of the microsporidian isolates was oval and the sige of the spore isolated from yellow-hind winged was measured in 2.6$\times$1, 5$\mu$ and that of the isolated from cutworm was measured in 3.7$\times$20.$\mu$. Both the isolated microsporidians showed the infectivity to silkworms, but the isolates did transovarian transmission in silkworms and the silkworm moth infected with the isolate from yellow-hind winged laid eggs irregularly.

• 방재와보험
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• s.101
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• pp.56-59
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• 2004

본 내용은 APE(ASIA PACIFIC FIRE) 2003년 12월호의 William S. FINK씨의 기사 일부를 요약, 보충하여 소개하는 위험관리정보입니다. 전체적인 손실제어시스템에서 다양한 공장 하위시스템들을 확인하기 위한 것으로, 하위시스템의 상호 관계를 분리하거나 완전히 독립적으로 웅영하는 것은 매우 어려우며 효율적으로 작동하지 않을 수도 있어, 이를 방지하기 위해서 전체시스템에 기초를 두고 투자검토하여야 필요없는 방재지설에 낭비하는 것을 막을 수 있다고 설명하고 있다.

• Journal of Korean Society of Water and Wastewater
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• v.18 no.5
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• pp.619-627
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• 2004

Purposes of this study were to examine closely the extracellular polymeric substances (EPS) which was a membrane fouling contaminant, to control detected EPS by powdered activated carbon (PAC) dosage etc. and to evaluate the possibility of practical reuse facility. With high removal efficiency of general pollutants, when the PAC is added to MBR, improvement of removal efficiency of $COD_{cr}$, and color was expected and treated wastewater can be reused. It was judged that the correlation between EPS and membrane fouling was very high. Carbohydrate and DNA in the EPS were judged to be cause of membrane fouling. If EPS could be controled, not only membrane fouling would be decreased but also operation time would be extended. In experiment of powdered activated carbon (PAC), characteristics of the best PAC for membrane fouling control were the particle size of $7{\mu}m$, lodine Number of 1,050, surface area of peat of $1,150m^2/g$. In lab test, operation time of MBR by PAC dosage of 200mg/gVSS was longer than one of MBR by without PAC dosage. Because EPS, especially carbohydrate and DNA, was controled successfully by PAC, membrane fouling in MBR could be decreased.

• Journal of Korean Society of Water and Wastewater
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• v.19 no.1
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• pp.38-46
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• 2005

In this research, we tried to combine the coagulation/sedimentation process as pre-treatment with UF membrane filtration to reduce the membrane fouling and to improve the permeate water quality. We used the Jet Mixed Separator (JMS) as coagulation/sedimentation process. We observed that the HPC and E.Coli can't be removed through the direct UF memebrane filtation of surface water. The removal efficiency of dissolved organic substances, indicated by E260 and DOC, was 40% and 15%, respectively. However, the removal efficiency of it increased two time as a result of combination of JMS process as coagulation/sedimentation pre-treatment. This was resulted from the formation of high molecular humic micro-floc through JMS process. The accumulation amount of irreversible cake layer which was not removed by backwashing was less than direct UF membrane filtration of surface water. Moreover, the loading rate of fouling induced substances, such as humic substances and suspended substances, on membrane surface decreased drastically through JMS process. As a result, the accumulation amount of irreversible cake on membrane surface was decreased.

• Journal of Korean Society of Water and Wastewater
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• v.26 no.6
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• pp.787-796
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• 2012

The purpose of this study is to investigate CSOs(combined sewer overflows) control in the combined sewer with/without separation wall. There is the high correlation between sewage velocity and suspended solid(SS) loading in the sewer without it. The SS/BOD ratio was about 3 times in the area with it, while it was about 5 times in the area without it. Therefore, the accumulated deposit within the sewer has influenced high SS loading in the sewer without it. This study showed that the separation wall installed acquired an acceptable efficiency in controlling the accumulated deposit in the combined sewer. According to this study, the BOD control effect was about 38 % in the sewer with the separation wall, whereas it showed about 24 % in the sewer without it. In this case, it was anticipated that the high pollutant control effect would be expected if the separation wall was installed in the combined sewer.

• Journal of Korean Society of Water and Wastewater
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• v.34 no.2
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• pp.93-104
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• 2020

As water resources are limited and legal regulations are strengthened, there is a growing need to reuse residuals in WTP(Water Treatment Plant). In this study, membrane filtration system was constructed and its operation method was studied for water quality stabilization and reuse of WTP residuals. The operation parameters were stable for 1 year and 6 months. Membrane fouling was identified as particulate pollution (activated carbon) and inorganic pollution (manganese). The membrane system was operated steadily with raw water of high concentration SS(Suspended solid) containing activated carbon because membrane fouling was reduced by the effect of End-Free type. In the case of inorganic contamination, dissolved manganese eluted by chemicals and acted as a membrane fouling source, and the operating conditions for minimizing membrane fouling. were confirmed by newly developing application methods and types of cleaning chemicals. Based on the results, design parameters for reducing manganese membrane fouling were derived.

• Journal of Korean Society of Water and Wastewater
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• v.22 no.3
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• pp.359-366
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• 2008

The series of experiments under the various conditions were carried out to evaluate the feasibility of dissolved air flotation (DAF) as an alternative of conventional gravity sedimentation (CGS) and to investigate the decrease of the loadings following to biological wastewater treatment processes in livestock wastewater system. On the basis of the experiment result between CGS and DAF processes, for the other water quality criteria as well as suspended solid the removal efficiency of DAF process was about 20~25 % better than CGS process on average. In addition, the particle removal efficiency of DAF process became higher in proportion as the increase of air to solid (A/S) ratio and the general wastewater treatment efficiency of DAF process was enough to meet the requirement of loading decrease to following biological process even at low A/S ratio range. Though DAF process is widely known as an solid separation unit, there was not the notable relationship between particle separation efficiency and several pollutant removal efficiencies like $COD_{Cr}$ and nutrients (T-N, T-P). Assume that the $COD_{Cr}$ was removed as the fraction of particle separation in this experiment, the removal efficiency of T-N and T-P were sensitive to removal efficiency of $COD_{Cr}$, especially.

• Journal of Korean Society of Water and Wastewater
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• v.32 no.5
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• pp.453-460
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• 2018

The impact of sodium hydroxide, which is one of chemicals of clean in place (CIP) for removing membrane fouling, on the PVDF membrane is reviewed with respect to physical/chemical structural change, the permeability affected therefrom. Based on the cleaning concentration applied in membrane water treatment facilities, 10% of accumulated defluorination was confirmed up to 166g.hr/L which reflects the exposure time. However, membrane resistance was confirmed to be reduced by about 10%. Through FT-IR and EDS analysis, reduction of F and change of are confirmed as factors that affect the permeability of membrane. Membrane resistance, which affects permeability, is affected by loss of additives for hydrophilicity, rather than defluorination of PVDF material. Therefore, in order to check membrane degradation degree, an accelerated test by NaOH was carried out, loss of additives was confirmed, and then PVDF inherent characteristic was observed.