• Journal of the Korean Chemical Society
• /
• v.50 no.2
• /
• pp.116-122
• /
• 2006

In this study we extracted DNA from 100 tissues of breast cancer patients and 103 normals. Then we confirmed single-nucleotide polymorphism(SNP) using PCR-DHPLC(polymerase chain reaction-denaturing high performance liquid chromatogrphy).Also, we studied SNP of samples using several columns to identify relation between packing materials of column and resolution.As a result, we identified 4 C/A, C/G genotypes(4%) in exon 5 and 37 T del genotypes(37%) in exon 8 among 100 breast cancer tissues and 2 in exon 5, 9 in exon 8 among 103 normal samples.In resolution test, we confirmed that PS-DVB(poly styrene-divinylbenzen) column is more efficient than C18 column.

• Korean Journal of Microbiology
• /
• v.36 no.1
• /
• pp.58-63
• /
• 2000

A bacterial isolate capable of degrading benzoate was selected from wastewater of Yocheon industrial complex and examined its biochemical characteristics and fatty acid composition. The isolate was identified as Klebsiella oxytoca strain C302. The strain C3O2 degraded catechol, protocatechuate, and 4-hydroxybenzoate as well as benzoate. The strain grew on and degraded 0.5 to 1.0 mM catechol most actively in MM2 medium at pH 7.0 and $30^{\circ}C$.

• KOREAN POULTRY JOURNAL
• /
• v.34 no.3 s.389
• /
• pp.132-133
• /
• 2002

• KOREAN POULTRY JOURNAL
• /
• v.34 no.2 s.388
• /
• pp.132-133
• /
• 2002

• KOREAN POULTRY JOURNAL
• /
• v.34 no.4 s.390
• /
• pp.115-117
• /
• 2002

• Korean Journal of Microbiology
• /
• v.44 no.3
• /
• pp.258-263
• /
• 2008

Late blight, one of the most important disease in many agricultural crops, is caused by Phytophthora infestans. Fusarium wilt is a vascular disease of many plants caused by Fusarium oxysporum. Some bacteria isolated from rhizosphere were screened for their ability to inhibit the growth of F. oxysporum and P. infestans. Productions of siderophore, $\beta-1$,3-glucanase, hydrogen cyanide and chitinase by 4 isolated strains were examined. Among them, Bacillus sp. RFO41 most effectively inhibited the growth of F. oxysporum. The highest productions of siderophore and $\beta-l$,3-glucanase were shown in the culture of Bacillus sp. RFO41. Bacillus strain PS2 was most effective against P. infestans. PS2 showed the highest production of chitinase and hydrogen cyanide. A significant relationship was shown between the antagonistic effects of isolates against F. oxysporum and P. infestans and their production level of siderophore, $\beta-1$,3-glucanase, hydrogen cyanide, and chitinase.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.43 no.8
• /
• pp.1289-1295
• /
• 2014

The aim of this study was to select the most suitable starter cultures for production of fermented sausages. A total of 27 strains isolated from Korean fermented foods and natural substances were characterized with respect to their physicochemical properties in a fluid (submerged) model system modified according to the special conditions of fermented sausages. Three of these strains were pre-selected for testing as potential cultures based on their ability to grow fast and initiate rapid acidification. The selected strains were identified by API and partial sequence analysis of 16S rRNA. The results exhibited sequence similarity to known sequences of Staphylococcus warneri, Staphylococcus epidermidis and Lactobacillus plantarum. Among them, relatively good growth properties and nitrite reduction activities were detected for S. epidermidis and L. plantarum and low pH values and high total acidities were observed in the model system fermented with these isolates compared with reference strains.

• Analytical Science and Technology
• /
• v.10 no.5
• /
• pp.325-331
• /
• 1997

Among various CE separation methods, micellar electrokinetic capillary chromatography(MECC) method using sodium dodecylsulfate(SDS) provides rapid and accurate separation of organic explosive constituents with easy. The running buffer was composed with 2.5 mM borate and 25mM SDS(pH 8.5). Addition of 1M urea and 10% organic modifiers (acetonitrile, methanol and ethanol) improves the resolution of adjacent explosive constituents. When 15 explosive constituents were developed in MECC, most constituents were separated successively while RDX/TNB and DNN/DEP were not, and detection limits of separated compounds are in range of 1 to 4 ppm.

• Food Science and Preservation
• /
• v.24 no.7
• /
• pp.1043-1051
• /
• 2017

Wild yeasts were isolated from domestic non-sterilized Makgeolli and their fermentation characteristics were analyzed to select the best fermentation seed culture. A total of 65 yeast strains isolated yeasts from non-sterilized Makgeolli and Nuruk. In order to select fermentable strains, hydrogen sulfide, $CO_2$ production ability, alcohol tolerance and aroma component production ability were analyzed. To screen the aromatic strains of isolates, media containing cerulenin, 5,5,5-trifluor-DL-leucine (TFL) and API ZYM kit were used. There were 36 strains resistance to cerulenin and all strains produced esterase and demonstrated tolerance against TFL. Hydrogen sulfide, which could degrade the quality of the fermented beverage, was not produced in 34 yeast. The correlation between alcohol tolerance of yeast and carbon dioxide production was analyzed by principal component analysis. YM22, YM31, YM32 and YM37 produced a total of 0.14-0.18 g/72 h of $CO_2$ indicating high fermentability. Alcohol tolerance was measured by alcohol concentration. YM32, YM37 yeast had 20% alcohol tolerance. As a result, alcohol and flavor characteristics of wild yeast isolated from non-sterilized Makgeolli were analyzed and it was confirmed that yeast was suitable for the production of alcohol.

• Journal of the Korean Applied Science and Technology
• /
• v.34 no.2
• /
• pp.271-279
• /
• 2017

This study evaluated antimicrobial efficacy and antioxidant effect of fraction isolated from Coptis Radix and confirmed its possibility as a cosmetic material. The extracts of isolated from Coptis Radix conducted an antimicrobial activity against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Candida albicans by disc diffusion method and measure clear zone. As a result, it was confirmed that antimicrobial activity against S. aureus and candida. A was observed in all samples except Fr 1. The activity of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and The activity of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) cation radical scavenging were determined by antioxidant assay according to the concentrations (50, 125, 250) ${\mu}g/mL$ of extracts of isolated from Coptis Radix. As a result, DPPH radical scavenging activity of Fr 1, 2, 3, 4 at $250{\mu}g/mL$ was 11.4%, 30.3%, 42.0% and 53.1%, respectively and $ABTS^+$ radical scavenging activity was 28.6%, 96.2%, 98.6% and 97.1% at the same concentration, respectively. Fr. 3 and 4 showed higher radical scavenging activity than the positive the control group BHT at the same concentration. In the WST assay results of measuring the cytotoxicity of Coptis Radix, except for Fr. 4, Fr. 1, 2 and 3 did not show toxicity. As a result, the fractions isolated from Coptis Radix can be regarded as a cosmetic material having antimicrobial activity and antioxidant ability.