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http://dx.doi.org/10.5012/jkcs.2006.50.2.116

The Development of Analysis System for Genes Related Disease Using Chemical Properties of DHPLC  

Kim, Jong-Gyu (단국대학교)
Nam, Yun-Hyeong (단국대학교)
Park, Sang-Beom (단국대학교)
Lee, Jae-Sik (단국대학교)
Gang, Won (단국대학교)
Publication Information
Journal of the Korean Chemical Society / v.50, no.2, 2006 , pp. 116-122 More about this Journal
Abstract
In this study we extracted DNA from 100 tissues of breast cancer patients and 103 normals. Then we confirmed single-nucleotide polymorphism(SNP) using PCR-DHPLC(polymerase chain reaction-denaturing high performance liquid chromatogrphy).Also, we studied SNP of samples using several columns to identify relation between packing materials of column and resolution.As a result, we identified 4 C/A, C/G genotypes(4%) in exon 5 and 37 T del genotypes(37%) in exon 8 among 100 breast cancer tissues and 2 in exon 5, 9 in exon 8 among 103 normal samples.In resolution test, we confirmed that PS-DVB(poly styrene-divinylbenzen) column is more efficient than C18 column.
Keywords
p53 Gene, Breast Cancer, PCR-DHPLC, Mutation
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