• Title/Summary/Keyword: 분리공시

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목초 생산성 향상을 위한 엔도파이트 이용

  • 이종경;윤세형;박근제;이성철
    • Proceedings of the Korean Society of Grassland Science Conference
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    • 1999.06a
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    • pp.96.1-96
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    • 1999
  • 본 연구는 주요 목초에서 엔도파이트가 생성하는 물질을 구명하고, 안정수준의 물질을 생산하는 우수종을 분리 접종하여 환경저항성 식물을 개발하려고 수행하였다. 공시재료는 톨 페스큐 5 품종을 선발 공시하였으며, 엔도파이트 비율과 품종의 생육특성, 수량과 사료가치를 비교하였다. 주요 연구결과로는 톨 페스큐 품종 중 엔도파이트 비율이 29%인 Barvetia가 초장과 엽폭이 크고, 녹색도가 우수하였다.(중략)

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Studies on the Malic Acid Degradation in Wine by Yeast (Part 2) Zymologic Characteristics of Schizosaccharomyces japonicus var. iaponicus (효모에 의한 과실주중의 감산효과에 관한 연구 (제2보) Schizosaccharomyces japonicus var japonicus의 양조학적 성질)

  • 유대식
    • Microbiology and Biotechnology Letters
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    • v.6 no.1
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    • pp.27-31
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    • 1978
  • The malo-alcoholic fermentation characteristics of Schiozaccharomyces japonicus var. japonicus which was freshly isolated from strawberry were studied. A good assimilation of malic acid by the strain was observed under the following conditions:pH 4.2∼4.8, alcohol content less than 12%, sulfur dioxide concentration less, than 150 ppm, and less than 0.01% of Mn$\^$2+/ as MnSO$_4$. The strain could remove 0.3% of malic acid completely in 6 days under stationary culture at 30$^{\circ}C$. Shaking the culture could promote growth of the strain but did not affect on the malo-alcoholic fermentation.

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Pathogenicity of Didymella bryoniae on the Seedlings of Cucurbits (오이류 유묘에 대한 덩굴마름병균의 병원성)

  • Lee Du Hyung
    • Korean Journal Plant Pathology
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    • v.1 no.3
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    • pp.173-177
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    • 1985
  • The objective of the study is to determine differences between cucurbits in the pathogenicity of Didymella bryoniae isolated from the naturally infected seeds of cucumber and pumpkin. Primary seedling infection of cucumber(Cucumis sativus L.), oriental melon(Cucumis melo var. makuwa Makino), pumpkin(Cucurbita pepo L.) and watermelon (Citrullus vulgaris Shrad.) occurred on the radicle, hypocotyl and cotyledons and symptoms on each crop were very similar. Infection of the radicle generally caused pre-emergence rot, while infection on the hypocotyl and cotyledons provided further inoculum for infection of the first true leaves and the stem. In cross inoculation tests, all isolates of D. bryoniae could infect cucumber, oriental melon, pumpkin and watermelon at different growth stages and there were not much differences in pathogenicity or susceptibility between isolates of the pathogen and crops tested. The susceptibility of cucumber and pumpkin was markedly influenced by prevailing humid conditions.

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Inhibition Effect of the Extracts of Trichloma matsutake Mycelia on Tyrosinase Activity (송이[Tricholoma matsutake]에서 분리된 균사 배양액의 Tyrosinase 억제효과)

  • 우현정;양덕조
    • KSBB Journal
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    • v.18 no.1
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    • pp.45-50
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    • 2003
  • In this study, the optimum conditions for mycelium culture of the mushroom Tricholoma matsutake and the inhibitory effect of the mycelium extracts no tyrosinase activity have been examined. When the extracts of the Tricholoma matsutake mycelia were tested for inhibitory activity on tyrosinase, it was found that the components extracted with ethyl acetate and water showed the highest inhibitory activity. The effect of antioxidants on the growth of mycelium and tyrosinase-inhibiting activity was also investigated. The results showed that tocopherol inhibited the growth in a concentration-dependent manner. In terms of tyrosinase-inhibiting activity, however, tocopherol was found to enhance the inhibitory activity.

Molecular Approaches to Determine the Character of Serratia marcescens Associated with the Insect Pathogenicity to Brown Planthopper (Serratia marcescens의 곤충 병원성 관련형질 탐색을 위한 분자생물학적 연구)

  • 김희규;배동원;박진희;윤한대
    • Korean journal of applied entomology
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    • v.32 no.3
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    • pp.330-337
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    • 1993
  • A bacterium, pathogenic to Nilaparvata lugens Stal. causing high mortality in 3~5 days, were selected and identified as Serratuz marcescens biotype A2a which is not a nosocomlally infective strain. In order to determine the characters of Serratia marcesce'1lS associated with insect pathogenicity, Tn5 mutagenesis was carried out by conjugating with E. coli pJB4J1. Transconjugants were plate-assayed for missing chitinase, protease and DNase activity. A protease negative mutant was selected for missing JOseet pathogenicity. SEM and TEM revealed the presence of bacterial cells in the epithelial tissue of inner abdomal tissue of the hypodermic layer of abdomen. Such a colonization was limmited to the subjacent tissue inside the intacL cuticular epidermis. These observation supported our result of pathogenicity tests of transconjugants.

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Occurrence of Sclerotinia Rot on Cucurbitaceous Vegetable Crops in Greenhouses (온실재배 박과 채소작물에서의 균핵병 발생)

  • Kim, Wan-Gyu;Cho, Weon-Dae;Jee, Hyeong-Jin
    • The Korean Journal of Mycology
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    • v.27 no.3 s.90
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    • pp.198-205
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    • 1999
  • Cucurbitaceous vegetable crops grown in greenhouses in Korea were surveyed from 1995 to 1997. Incidence of Sclerotinia rot was as high as $30{\sim}70%$ at its maximum on Cucumis melo var. reticulatus (netted melon), Cucumis sativus (cucumber), Cucurbita moschata (pumpkin) and Cucurbita pepo (summer squash) but relatively low on Citrullus lanatus (watermelon) and Cucumis melo var. makuwa (oriental melon). Symptoms of Sclerotinia rot developed on stems of all the cucurbits, fruits of five cucurbits except C. lanatus, petioles of two Cucurbita spp. and leaves of C. moschata. A total of 126 isolates of Sclerotinia sp. were obtained from the lesions and identified as Sclerotinia sclerotiorum based on the morphological and cultural characteristics. The fungus was very frequently isolated from stems and fruits of the cucurbits but rarely from petioles and leaves. Six isolates of the fungus were pathogenic to six cucurbits tested although there was some difference in virulence among the isolates to some of the hosts. C. lanatus was the most susceptible to the isolates, whereas C. melo var. makuwa was the most resistant. C. melo var. reticulatus and C. sativus were relatively susceptible to the isolates, and C. moschata and C. pepo relatively resistant.

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Host Range of Isolates of Magnaporthe grisea (한국에서 분리한 벼 도열병균의 기주범위)

  • 최우봉;전삼재;이용환
    • Korean Journal Plant Pathology
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    • v.12 no.4
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    • pp.453-454
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    • 1996
  • 벼, 바랭이, 톨페이스큐로부터 분리한 벼 도열병균에 대한 기주범위를 벼, 밀, 보리, 옥수수, 호밀, 귀리, 톨페스큐 등의 작물과 피, 왕바랭이, 바랭이, 강아지풀, 개기장 등의 잡초를 대상으로 실험하였다. 대부분의 균주들은 공시작물 모두와 잡초인 강아지풀에대하여 병원성을 나타내었다. 이러한 결과는 논 주면의 강아지풀이 벼 도열병균의 기주로 작용할 수 있음을 나타내었다.

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Isolation and Identification of Antifungal Substances Produced by Fusarium sp. ByA-1 (Fusarium sp. BYA-1 균주가 생성하는 항진균성 항생물질의 분리 및 동정)

  • 서영수;김진철;김병섭;이인원;조광연
    • Korean Journal Plant Pathology
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    • v.12 no.1
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    • pp.72-79
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    • 1996
  • 보리로부터 분리한 Fusarium sp. BYA-1균주의 감자한천배지 배양체로부터 여러 식물병원곰팡이에 길항력을 나타내는 세 개의 항생물질을 분리하였다. 추출한 세 개의 항생물질은 silica gel관 크로마토그래피와 분취 HPLC, 그리고 Phytolhthora capsici 검정을 이용하여 정제하였다. 이들 분리한 항생물질들을 동정하기 위하여 융점 결정, 자외선흡광법, 질량분석 및 핵자기공명법 등의 기기분석을 실시하였다. 그 결과, 세 개의 항진균성 항생물질들은 fusarielin A, enniatin B, 그리고 enniatin B\ulcorner으로 각각 동정되었다. 분리한 세 개의 물질 중 fusarielin A가 공시된 곰팡이에 가장 강한 항균활성을 나타내었으며, 최소저해농도는 40$\mu\textrm{g}$/ml이하였다. Fusarium속 균주가 구조적으로 다른 두 종류의 항진균성 항생물질인 fusarielin A와 enniatins을 동시에 생성한다는 것은 본 논문에서 처음으로 보고하는 것이다.

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Characterization and Enterotoxigenicity of Staphylococcus aureus Isolated form Patient and Healthy Human (환자 및 건강인 유래 Staphylococcus aureus의 특성과 Enterotoxin 산생성)

  • 최홍근;손원근;강호조
    • Journal of Food Hygiene and Safety
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    • v.6 no.2
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    • pp.89-93
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    • 1991
  • The present study was conducted to investigate the prevalence, the biochemical properties and the enterotoxin types of Staphylococcus aureus in healthy human and patient. A total of 61 S. aureus strains were isolated from 142 samples. The prevalence of S. aureus isolated from healthy human and patient were 17.7% and 14.0%, respectively. All the isolates showed the production of coagulase, lecithinase and hemolysis (${\alpha}-hemolysis;\;32.8%,\;{\beta}-hemolysis;\;67.2%$) on sheep blood agar. Coagulase type VII (38.4%) and type 111 (26.0%) were dominant among coagulase types I through VIII. Twenty-four (52.2%) of 46 strains tested produced one or more enterotoxin; enterotoxin A, Band C were produced by 3, 9 and 12 strains, respectively. Enterotoxins were produced by 100% of type 11 strains, 75% of type 111 and 39.1 % of type VII. Commonly, coagulase type II produced enterotoxin B or C, and type VII produce enterotoxin C.

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The political economy analysis of the mobile phone subsidy (이동통신 보조금의 정치경제학적 분석)

  • Shin, Jin
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.19 no.8
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    • pp.1893-1900
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    • 2015
  • The government has the responsibility to prevent abuses of monopoly and promote competition in order to maximize consumer welfare. The government should address the asymmetry in the information as much as possible and ensure consumer choice. The subsidy seems to reduce the burden on the consumer service charges and handset prices but it actually distorts the market through price discrimination. The government caused the principal-agent problem by neglecting their appropriate role to prevent distortion of the mobile telecom market. The money used as subsidy is part of excess profit and could be transferred to down the price of mobile phone and charges and it would become a benefit to consumers. Separate announcement of subsidies by Mobile Communications Terminal Distribution Structure Improvement Act is a little developed policy but it was not actually realized. The market price close to that from perfect competition structure is plausible, ultimately.