• Title/Summary/Keyword: 부착 실험

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Analysis of Enterococcus faecalis Attachment to Granular Activated Carbon with a Column Experiment (칼럼실험에 의한 입상활성탄에서 Enterococcus faecalis의 부착 연구)

  • Kim, Hyon-Chong;Park, Seong-Jik;Lee, Chang-Gu;Han, Yong-Un;Kim, Song-Bae
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.2
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    • pp.119-124
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    • 2009
  • The aim of this study was to investigate the influence of ionic strength and iron impregnation on the attachment of Enterococcus faecalis to granular activated carbon (GAC). Column experiments were performed to examine bacterial adhesion to coconutbased GAC (c-GAC), iron-impregnated c-GAC (fc-GAC), acid-washed c-GAC (a-GAC) and iron-impregnated a-GAC (fa-GAC) under two different solution (NaCl 1, 10 mM) conditions. Results showed that bacterial mass recovery in c-GAC decreased from 77.3 to 61.6% while in a-GAC it decreased from 71.6 to 32.3% with increasing ionic strength from 1 to 10 mM. This indicates that bacterial attachment to GAC can be enhanced with increasing ionic strength. Results also showed that the mass recoveries in fc-GAC were 62.6% (1 mM) and 53.3% (10 mM) while they were 50.8% (1 mM) and 16.9%(10 mM) in fa-GAC, which were lower than those in c-GAC and a-GAC. This demonstrates that bacterial adhesion to GAC can be enhanced through iron impregnation. This study provides information regarding the effects of ionic strength and iron impregnation on bacterial attachment to GAC. Furthermore, this study will advance our knowledge of bacterial removal in surface-modified granular media.

Effect of Recycled Coarse Aggregate (RCA) Replacement Level on the Bond Behaviour between RCA Concrete and Deformed Rebars (순환 굵은골재의 혼입률에 따른 콘크리트와 이형철근의 부착 거동)

  • Jang, Yong-Heon;Choi, Ki-Sun;You, Young-Chan;Kim, Keung-Hwan;Yun, Hyun-Do
    • Journal of the Korea Concrete Institute
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    • v.22 no.1
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    • pp.123-130
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    • 2010
  • In this study, mixed recycled coarse aggregate (RCA) was produced by mixing RCA from waste concrete in order to evaluate a new method of RCA production. Bond strength between reinforcing bars and RCA concrete was qualitatively evaluated as a part of continuous studies to establish design code of reinforced concrete structural members using recycled aggregate. For practical application, specimens were manufactured with the ready mix RCA concrete. Parameters investigated include: concrete compressive strength (i.e 21, 27 and 40 MPa), replacement levels (i.e 0, 30, 60 and 100%), bar position (i.e vertical and horizontal) and bar location (75 and 225 mm). For the pull-out test, each specimen was in the form of a cube, with each side of 150 mm in length and a deformed bar, 16 mm in diameter, was embedded in the center of each specimen. From the test results, the most of HT type specimen with compressive strength of 21 and 27 MPa showed lower bond strength than the ones provided in CEB-FIP and considered in reinforcement location factor ($\alpha\;=\;1.3$). It was reasoned that bonded area of top bar specimen was reduced at the soffit of reinforcement because of bleed water of fresh concrete. Therefore the reinforcement location factor in current KCI design code should be reviewed and modified.

Attachment of Bacillus subtilis to Al-Fe Bimetallic Oxide-coated Sand : Effect of Oxyanions (알루미늄.철 산화물 동시피복모래에서 Bacillus subtilis의 부착: 산화음이온의 영향)

  • Park, Seong-Jik;Lee, Chang-Gu;Han, Yong-Un;Park, Jeong-Ann;Kim, Song-Bae
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.7
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    • pp.515-520
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    • 2009
  • This study investigated the influence of oxyanions (nitrate, carbonate, phosphate) on the attachment of bacteria (Bacillus subtilis) to Al-Fe bimetallic oxide-coated sand using column experiments. Results showed that bacterial attachment to the coated sand was independent of nitrate concentration. Bacterial mass recovery remained constant (10.9${\pm}$0.2%) with varying nitrate concentrations (0.1, 1, 10 mM). In case of carbonate, mass recovery increased from 25.6% to 39.0% with increasing carbonate concentration from 0.1 mM to 1 mM, and mass recovery also increased from 50.9% to 78.9% at the same concentration condition in case of phosphate. This phenomenon could be attributed to the hindrance effect of carbonate and phosphate to bacterial attachment to the coated sand. Meanwhile, with increasing carbonate/phosphate concentration from 1 mM to 10 mM, mass recovery decreased from 39.0% to 23.8% and from 78.9% to 52.6%, respectively. This phenomenon could be ascribed to the enhancement effect of free carbonate/phosphate ions present in solution phase due to increasing carbonate/phosphate concentration, which increase ionic strength and thus enhance bacterial attachment to the coated sand. In our experimental conditions, the effect of phosphate to bacterial attachment to the coated sand was the greatest among phosphate, carbonate, and nitrate.

Experimental Study on the Precast Concrete Joint using Re-bar Debonding and Cutting Technique (철근 비부착 및 절단 기법을 사용한 PC 접합부의 실험적 연구)

  • Yi, Waon-Ho;Moon, Jung-Ho;Lee, Yong-Jae;Lee, Han-Jun
    • Journal of the Korean Society of Hazard Mitigation
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    • v.2 no.4 s.7
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    • pp.131-141
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    • 2002
  • Precast concrete (PC) panels are often used as retaining walls to support soil pressure. In such a case, the panels should be connected at the location where PC panels meet with a buttress. However, it is not easy to provide enough development length for the reinforcing steels due to the limited width of the buttress. If it happens, the width of buttress should be increased as large enough although it is not desirable. The critical section required for providing the development length is always located where the flexural moment is maximum. Thus it is the place the buttress width ends. Also it is the place that the reinforcing steels stressed to maximum. However, it is possible to make differentiate between the maximum moment location and the most stressed location of reinforcing steels. It means that the most stressed location of reinforcing steels, the critical section, can be moved to the other place where the moment is not maximum. New critical location will have less moment than that of buttress width ends. In consequence, the development length would be longer than that of the typical way of construction. Debonding or cutting technique make it possible to reduce the moment strength of a section. Therefore reinforcing steels are debonded or cut to have a desired flexural strength at a desired place. In this study, five test specimens in full scale were erected to examine the effects of critical section movement in PC panel joints. Test parameters were the length variations of debonded and cut reinforcing steels. The test results showed that the debonding or cutting technique could be used to lengthen the development length in the joint of PC panels.

Computational Fluid Analysis for the Otter Boards - 4 . Efficiency Analysis of the Cambered Otter Boards for the Vortex Generators - (전개판에 대한 수직해법 - 4 . 과발생기에 따른 만곡형전개판의 성능분석 -)

  • 고관서
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.27 no.4
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    • pp.286-292
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    • 1991
  • The authors propose to use the vortex generators in order to improve of the efficiency for the cambered otter boards. The equipments and testing method of this model test was the same as the previous report. This study was tested for 6 models such as the single cambered, the V-shaped cambered and the slotted cambered otter board without and with vortex generators. The results obtained are as follows: \circled1 C sub(L) of the single cambered model otter board with vortex generators was increased about 10% in comparison with that of model without vortex generators, C sub(D) decreased 2%, and L/D increased 5~20%. \circled2 L/D of the V-shaped cambered model otter board with vortex generators was increased 10~20% in comparison with that of model without vortex generators. \circled3 C sub(L) of the two slotted cambered model otter board with vortex generators was increased about 20% within an angle of attack 25$^{\circ}$ in comparison with that of without vortex generators, C sub(D) increased 5~20%, and L/D was higher than prototype within an angle of attack 20$^{\circ}$. \circled4 The separation point of the model otter boards with vortex generators was removed back ward a little in comparison with that of the model without vortex generators. \circled5 Flow speed difference of the back side to the front side of model otter boards with vortex generators was increased a little in comparison with that of the models without vortex generators. \circled6 The size of separation zone in case of the model otter boards with vortex generators was decreased about 10% in comparison with that of the models without vortex generators.

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Transmitted Noise Reduction of Piezoelectric Smart Panels using Passive/Active Method in Wide Range frequency (수동/능동적 방법을 혼용한 압전지능패널의 광대역 전달 소음저감성능)

  • 이중근;박우철
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.2 no.2
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    • pp.73-79
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    • 2001
  • In this paper, the transmitted noise reduction performance of piezoelectric smart panels is experimentally studied. The proposed piezoelectric smart panels are comprised of plate structure on which piezoelectric sensor/actuators are bonded and sound absorbing material is provided. It is a combination of passive and active approaches utilizing a passive effect at high frequencies and an active effect at low frequencies. To prove the concept of piezoelectric smart panels, an acoustic measurement experiment is performed. An acoustic tunnel is designed and its acoustic characteristics are tested. Below 800Hz, the tunnel exhibits a plane wave guide characteristics. When an absorbing material is bonded on a single plate, a remarkable transmitted noise reduction in mid frequency range is observed except the first resonance frequency. By enabling the active control of single smart panel with negative feedback control. about 10dB noise reduction is achieved at the resonance frequencies. The double smart panel got 4dB at the first resonance frequency and has more potential to reduce the transmitted noise in a wide range frequency. Piezoelectric smart panels incorporating passive absorbing material and active piezoelectric devices is a promising technology for noise reduction in a wide range frequency.

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Beam Tests for Static and Fatigue Interface Shear Strength between Old and Njew Concretes (신구콘크리트 계면의 전단강도 측정을 위한 정하중 및 피로하중 보실험)

  • 최동욱
    • Magazine of the Korea Concrete Institute
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    • v.9 no.3
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    • pp.137-147
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    • 1997
  • Interface shear strength of' concrete under static loading and deterioratiion of interface strength by fatigue loading in shear were experimentally investigated using composite beam test specimens. Thirteen beams were constructed. Five composite beams were tested statically until interface delaminations were observed in the static tests. Seven composite beam and one monolithically cast beam were subjected to two to three million cycles of fatigue load. Test variables were interface roughness, interface shear reinforcement, and presence of interface bond. The average interface shear strength of the composite beams with bonded-rough interface was 6, 060 kPa. No interface delamination was observed after cycling for the composite beams with bonded - rough interface and interface bond was not influenced by repeated application of the shear stress of 2.000 kPa(about 1/3 of the static interface shear strength). Smooth interface and unbonded-rough interface with shear reinforcement deteriorated under repeated shear loading.

An Experimental Study on the Behavior of RC Beams Externally Bonded with FRPs Under Sustained Loads (지속하중을 받은 FRP 외부부착 보강 철근콘크리트 보의 거동 특성에 관한 실험적 연구)

  • Shim, Jae-Joong;Oh, Kwang-Jin;Kim, Yeon-Tae;Park, Sun-Kyu
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.14 no.1
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    • pp.125-132
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    • 2010
  • In the recent construction industry, an external strengthening method using fiber reinforced polymers has been widely used. Since reinforced concrete structures strengthened with fiber reinforced polymers are always under sustained loads, influence of creep and shrinkage on the structures is inevitable. Due to the creep and shrinkage, behaviors of the structures, such as deflection, deformation, recovery capability, strength and so on are also under the influence of creep and shrinkage. Thus, in order to estimate efficacy, creep recovery and residual strength of FRP strengthened RC beams, long-term flexural experiments and static flexural experiments were carried out. As the result of the experiments, FRP strengthened RC beams were very effective in terms of deflection control. Furthermore, the strengthened beams had higher immediate deformation recovery than immediate deformation. Through the static flexural experiments, it was shown that the CFRP strengthened beam had high residual strength. It seems that the sustained loads did not affect bond and residual strength of the beams.

Collagen과 osteopontin이 도입된 고기능성 PCL membrane의 제조 및 지방유래 줄기세포의 골세포 분화능 관찰

  • Kim, Yeong-Hui;Song, Ho-Yeon;Jyoti, Anirban;Byeon, In-Seon;Park, Min-Ju;Lee, Byeong-Taek
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2010.05a
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    • pp.42.1-42.1
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    • 2010
  • Poly-carprolactone (PCL)은 생분해성 고분자로 장기간의 임상실험 결과 생체에 독성이 없으며 생체친화성이 우수한 소재로 확인되어 PLGA, PLLA 등과 더불어 조직공학 분야에서 널리 사용되고 있는 생체재료이다. 그러나 PCL은 5개의 비극성 methylene group과 1개의 극성 ester group이 반복되는 지방족의 polyester로 구조상 탄소수가 많아 소수성을 띄는 단점을 가지고 있다. 이러한 표면이 소수성인 재료의 경우, 초기 단백질 흡착능이 떨어져 세포의 부착이 느린 속도로 일어나므로 세포 분화 및 조직 재생이 더디게 일어난다. 본 연구에서는 소수성의 PCL 표면의 단백질 흡착능을 증가시키기 위해 기능성 amine group을 부착하였으며, 또한 골재생을 촉진시킬 수 있는 세포외 기질인 collagen과 osteopontin을 부착함으로써 고기능성 PCL membrane을 제조하였다. 제조된 PCL membrane은 골재생용 조직공학에의 응용을 위해 지방유래 줄기세포를 이용하여 부착능 및 골세포로의 분화능을 확인하였다. 표면 성질의 변화에 의한 세포의 부착능의 변화를 confocal microscopy을 이용하여 부착에 관여하는 단백질의 발현을 확인하였으며, collagen과 osteopontin에 의한 골세포로의 분화능을 확인하기 위해 real time PCR을 통해 골세포의 분화 표지 유전자의 발현을 비교 분석하였다.

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PERIODONTAL RESPONSE FOLLOWING APPLICATION OF INTRUSIVE FORCES TO THE INCISORS WITH BONE LOSS AND PERIODONTAL DISEASE IN ADULT DOGS (골결손과 치주질환 유도 후 성견 절치의 실험적 함입이동시 치주조직의 반응)

  • Hwang, Hyeon-Shik;Park, Yang-Soo;Choi, Hong-Ran
    • The korean journal of orthodontics
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    • v.28 no.3 s.68
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    • pp.431-440
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    • 1998
  • While periodontal disease results in the extrusion of upper incisors with interdental spacing in adult patient, the intrusive movement should be executed for the rehabilitation in terms of esthetics and function. The present study was peformed to investigate the periodontal response following orthodontic intrusion of teeth with infrabony pocket. Bone defect and periodontal disease were produced adjacent to the both maxillary second lateral incisors in four adult dogs. Four weeks later, a flap operation was performed to eliminate inflammation and a reference notch was made on the root surface at the level of bottom of each defect. Two weeks after periodontal surgery, 4 weeks of intrusion and subsequent another 4 weeks of retention was executed on the right side of incisors while left incisors served as the control. Through the histologic analysis, following results were obtained. 1. Histologic section of control side showed the reduction of pocket depth after flap operation. However, the status of long junctional epithelium was observed while new connective tissue attachment occurred in a small area adjacent newly formed cementum 2. Epithelial attachment was less around the intruded incisor while new connective tissue attachment was much more compared to the control side. 3. Plenty of cells were observed in the connective tissue around the reference notch of the intruded incisor and the degree of the new cementum formation was higher in the intruded incisor than the control. The results of the present study suggested that an orthodontic intrusion of periodontally involved and extruded incisors could be carried out with a desirable periodontal response, provided that inflammation is under control.

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