Journal of Korean Society of Environmental Engineers (대한환경공학회지)

Korean Society of Environmental Engineers (대한환경공학회)
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Attachment of Bacillus subtilis to Al-Fe Bimetallic Oxide-coated Sand : Effect of Oxyanions

알루미늄.철 산화물 동시피복모래에서 Bacillus subtilis의 부착: 산화음이온의 영향

  • Park, Seong-Jik (Environmental Biocolloid Engineering Laboratory, Seoul National University) ;
  • Lee, Chang-Gu (Environmental Biocolloid Engineering Laboratory, Seoul National University) ;
  • Han, Yong-Un (Environmental Biocolloid Engineering Laboratory, Seoul National University) ;
  • Park, Jeong-Ann (Environmental Biocolloid Engineering Laboratory, Seoul National University) ;
  • Kim, Song-Bae (Department of Rural Systems Engineering.Research Institute for Agriculture and Life Sciences, Seoul National University)
  • 박성직 (서울대학교 환경바이오콜로이드공학 연구실) ;
  • 이창구 (서울대학교 환경바이오콜로이드공학 연구실) ;
  • 한용운 (서울대학교 환경바이오콜로이드공학 연구실) ;
  • 박정안 (서울대학교 환경바이오콜로이드공학 연구실) ;
  • 김성배 (서울대학교 지역시스템공학과, 농업생명과학연구원)
  • Received : 2009.06.09
  • Accepted : 2009.07.06
  • Published : 2009.07.31
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Abstract

This study investigated the influence of oxyanions (nitrate, carbonate, phosphate) on the attachment of bacteria (Bacillus subtilis) to Al-Fe bimetallic oxide-coated sand using column experiments. Results showed that bacterial attachment to the coated sand was independent of nitrate concentration. Bacterial mass recovery remained constant (10.9${\pm}$0.2%) with varying nitrate concentrations (0.1, 1, 10 mM). In case of carbonate, mass recovery increased from 25.6% to 39.0% with increasing carbonate concentration from 0.1 mM to 1 mM, and mass recovery also increased from 50.9% to 78.9% at the same concentration condition in case of phosphate. This phenomenon could be attributed to the hindrance effect of carbonate and phosphate to bacterial attachment to the coated sand. Meanwhile, with increasing carbonate/phosphate concentration from 1 mM to 10 mM, mass recovery decreased from 39.0% to 23.8% and from 78.9% to 52.6%, respectively. This phenomenon could be ascribed to the enhancement effect of free carbonate/phosphate ions present in solution phase due to increasing carbonate/phosphate concentration, which increase ionic strength and thus enhance bacterial attachment to the coated sand. In our experimental conditions, the effect of phosphate to bacterial attachment to the coated sand was the greatest among phosphate, carbonate, and nitrate.

본 연구에서는 칼럼실험을 이용하여 알루미늄 철 산화물 동시피복모래에서 박테리아(Bacillus subtilis)의 부착에 산화음 이온(질산염, 탄산염, 인산염)이 미치는 영향을 조사하였다. 실험결과, 질산염의 경우 피복모래에서의 박테리아 부착은 질산염 이온의 농도변화와 무관하였다. 질산염의 농도가 변화함에 따라(0.1, 1, 10 mM) 질량회수율은 10.9${\pm}$0.2 %로 일정하였다. 탄산염의 경우 농도가 0.1 mM에서 1 mM로 증가함에 따라 질량회수율이 25.6%에서 39.0%로 증가하였고, 인산염의 경우에도 동일한 농도조건에서 50.9%에서 78.9%로 증가하였다. 이러한 현상은 박테리아 부착에 대한 탄산염과 인산염 이온의 방해효과 때문이었다. 반면, 탄산염/인산염의 농도가 1 mM에서 10 mM로 증가함에 따라 질량회수율이 각각 39.0%에서 23.8%로 78.9%에서 52.6%로 감소하였다. 이러한 현상은 탄산염/인산염의 농도증가에 따라 피복모래표면에 흡착되지 않고 수용액상에 존재하는 탄산염이나 인산염 이온이 이온강도를 증가시킴으로써 일어나는 박테리아 부착에 대한 증진효과 때문이었다. 본 실험조건에서 피복모래에서의 박테리아 부착에 미치는 영향은 인산염, 탄산염, 그리고 질산염 중, 인산염이 가장 큰 것으로 나타났다.

Keywords

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