• Korean Journal of Fisheries and Aquatic Sciences
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• v.22 no.4
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• pp.196-200
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• 1989

Fishery production of topshell in Cheju-do has been rapidly decreased in the last few years. To investigate the origin of this problem and estimate maximum sustainable yield (MSY) and catch per unit effort (CPUE) of topshell in Cheju-do, a survey has been done ysing the statistical data for 19 years (1968-1986) of topshell production in Cheju-do and data for 3 yea.5 (1984-1986) from an fishery village, Tonggui-ri, Pukcheju-gun, Cheju-do. Maximum sustainable yield of topshell in Cheju-do assumed to be 2,500-2,800 metric tons(M/T) per year. The annual fishery productions of topshell from 1982 to 1986 were 3,368, 3,649, 3,308, 3,136, and 1,400 M/T, respectively. These results show that topshell had been over-fished during 1982 to 1985. Because of the over-fishing, production sharply decreased down to 1,400 M/T in 1986. Total annual production of topshell in Tonggui-ri is a little less than one Percent of that of Cheju-do. To achieve this production, over the half of resistered diving women in this village have been worked. To estimate catch per unit effort of topshell in Cheju-do, it seems better to use the data which is the number of diving women who have been worked for certain period of time than the number of working days in the same period of time.

• The Transactions of the Korea Information Processing Society
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• v.13 no.3
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• pp.102-110
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• 2024

Blockchain records all transactions issued by users, which are then replicated, stored, and shared by participants of the blockchain network. Therefore, the capacity of the ledger stored by participants continues to increase as the blockchain network operates. In order to address this issue, research is being conducted on methods that enhance storage efficiency while ensuring that valid values are stored in the ledger even in the presence of device failures or malicious participants. One direction of research is applying techniques such as Reed-Solomon encoding to the storage of blockchain ledgers. In this paper, we apply Reed-Solomon encoding to the key-value store used for ledger storage in an open-source blockchain, and measure the storage efficiency and increasing computational overhead. Experimental results confirm that storage efficiency increased by 86% while the increase in CPU operations required for encoding was only about 2.7%.

• Korean Journal of Animal Reproduction
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• v.24 no.2
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• pp.217-222
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• 2000

This study was conducted to investigate the effect of quiescent treatment of the donor cells on the nuclear remodeling and in vitro development of fetal fibroblast cell-cloned bovine embryos. Serum starved, confluent and nonquiescent cycling fetal fibroblast cells were transferred into the enucleated oocytes. About 20∼25% of nuclear transfer embryos fused with a serum starved or confluent cell extruded a polar body, which was slightly lower than that of nontreated control (36%). About 49∼51% of nuclear transfer embryos fused with a serum starved or confluent cell had a single chromatin clump, which was slightly higher than that of nontreated control (40%). The proportion of embryos with a single chromatin clump was significantly higher (P＜0.01) in nuclear transfer embryos without showing a polar body (60.5%) than with a polar body (4.7%). Development rates to the blastocyst stage were 21.7% and 20.9% when serum starved and confluent cells were transferred, which were slightly higher than that of control (14.1 %). The result of this study suggests that quiescent treatment by serum starvation or growth to confluency of donor cells could increase the number of embryos with a normal chromatin structure, which results in increased in vitro development.

• Journal of Animal Science and Technology
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• v.50 no.5
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• pp.641-648
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• 2008

There has been great success for making transgenic animals using somatic cell nuclear transfer(SCNT) up to this time. However, the success rates of the production of live transgenic animals are still very low. The current research has been carried out for delineation of differentially expressed genes between SCNT and normal placenta in cattle. In the present observations, high expression has been observed for CTSZ, LOC509426 and ELF1 genes in normal placenta. On the other hand, TIMP2, PAG1B, PAG-21, LOC782894, SERPINB6 and mKIAA2025 protein were highly expressed in SCNT placenta. Five genes, which were highly expressed in SCNT placenta, have been further investigated using semi-quantitative real-time PCR. The results were similar to that we observed using ACP. In the future, all genes affecting the SCNT and normal placenta have to be discovered and their networks will be fully investigated. The genes were identified in this study would be great help for identifying differential gene expressions in SCNT placenta.

• Korean Journal of Animal Reproduction
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• v.23 no.3
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• pp.221-227
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• 1999

Successful cryopreservation of bovine immature oocytes can increase availably of oocytes for the in vitro fertilization or nuclear transfer. However, it was not reported successful development to the blastocyst stage following in vitro fertilization of cryopreserved bovine immature oocytes. The objective of this study was to determine the incidence of survival, meiotic maturation, fertilization and in vitro development of cryopreserved bovine immature by ultra rapid cooling methods. The oocytes were adversely affected by brief exposure to EFS40 solution in electron microscope grids and plunged directly into liquid nitrogen. After such ultra-rapid cooled immature oocytes were warmed, 78% of oocytes were matured to the metaphase II stage, 50% of oocytes were fertilized after insemination, and 5% of oocytes were developed to the blastocyst stage. Different sodium concentration of sodium ion in the freezing medium did not affect survival, maturation, fertilization and in vitro development of cryopreserved oocytes. These results suggested that immature bovine oocytes can be cryopreserved by ultra-rapid cooling methods.

• Magazine of the Korea Concrete Institute
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• v.3 no.4
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• pp.133-142
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• 1991

When the behavior of a prototype concrete structure is studied through small-scale model experiments, it is necessary to reproduce all significant physical characteristics on either an one-to-one basis or a specific similitude relationship. Any distortion of similitude must be understood and its effect must be predictable. This paper focuses on improved physical modeling techniques for small-scale reinforced concrete structures. Particular emphasis is placed on the development of a model concrete mix to accurately model the important properties of full-scale prototype concrete. Four types of model reinforcement with different bond characteristics are also studied by testing twenty simple beams. The information obtained will be of immediate use to engineers contemplating small-scale modeling of reinforced concrete structures.

• Journal of the Korean Society for Library and Information Science
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• v.38 no.1
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• pp.323-346
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• 2004

These days the number of copyright issues concerning libraries is increasing. The reason is a library is the place that collects and uses copyrighted materials. The copyright issues that libraries face will be more radical, because of the growing number of digital media especially appearance of a digital library. To make the current situation better, this study suggests the standard of copyright compliance policy for university libraries in order to serve within the range of copyright law. Copyright compliance policy is a reasonable guideline meeting user's intellectual needs and protecting a copyright holder's right.

• The Journal of the Acoustical Society of Korea
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• v.20 no.3
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• pp.3-7
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• 2001

In active sonar system using CW signal, when the noise included reverberation has not the white characteristics, the CFAR detector estimates high threshold. Because of this reason it cannot detect targets and not resolve the closely spaced multiple targets. In order to solve these problems, we propose an adaptive reverberation rejection filter The proposed filter is composed of an adaptive filter and a fixed filter with its coefficients. To study the performance of the proposed adaptive reverberation rejection filter, various experiments have been performed under In moving active sonar environments. As a results, the proposed method has the improved performance than the previous methods.

• Journal of Life Science
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• v.25 no.3
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• pp.349-356
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• 2015

Thanks to recent advances in next-generation sequencing (NGS) technology, diverse livestock species have been dissected at the genome-wide sequence level. As for cattle, there are currently four Korean indigenous breeds registered with the Domestic Animal Diversity Information System of the Food and Agricultural Organization of the United Nations: Hanwoo, Chikso, Heugu, and Jeju Heugu. These native genetic resources were recently whole-genome resequenced using various NGS technologies, providing enormous single nucleotide polymorphism information across the genomes. The NGS application further provided biological such that Korean native cattle are genetically distant from some cattle breeds of European origins. In addition, the NGS technology was successfully applied to detect structural variations, particularly copy number variations that were usually difficult to identify at the genome-wide level with reasonable accuracy. Despite the success, those recent studies also showed an inherent limitation in sequencing only a representative individual of each breed. To elucidate the biological implications of the sequenced data, further confirmatory studies should be followed by sequencing or validating the population of each breed. Because NGS sequencing prices have consistently dropped, various population genomic theories can now be applied to the sequencing data obtained from the population of each breed of interest. There are still few such population studies available for the Korean native cattle breeds, but this situation will soon be improved with the recent initiative for NGS sequencing of diverse native livestock resources, including the Korean native cattle breeds.

• Reproductive and Developmental Biology
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• v.35 no.1
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• pp.77-83
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• 2011

One-step dilution and direct transfer would be a practical technique for the field application of frozen embryo. This study was to examine whether Jeju Black Cattle (JBC, Korean Cattle) can be successfully cloned from vitrified and one-tep diluted somatic cell nuclear transfer (SCNT) blastocyst after direct transfer. For vitrification, JBC-SCNT blastocysts were serially exposed in glycerol (G) and ethylene glycol (EG) mixtures [10%, (v/v) G for 5 min., 10% G plus 20% EG (v/v) for 5 min., and 25% G plus 25% EG (v/v) for 30 sec.] which is diluted in 10% FBS added D-PBS. And then SCNT blastocysts were loaded in 0.25 ml mini straw, placed in cold nitrogen vapor for 3 min. and then plunged into $LN_2$. One-step dilution in straw was done in $25^{\circ}C$ water for 1 min, by placing vertically in the state of plugged-end up and down for 0.5 min, respectively. When in vitro developmental capacity of vitrified SCNT blastocyst was examined at 48 h after one-step dilution, hatched rate (56.4%) was slightly lower than that of control group (62.5%). In field trial, when the vitrified-thawed SCNT blastocysts were transferred into uterus of synchronized 5 recipients, a cloned female JBC was delivered by natural birth on day 299 and healthy at present. In addition, when the short tandem repeat marker analysis of the cloned JBC was evaluated, microsatellite loci of 11 numbers was perfectly matched genotype with donor cell (BK94-14). This study suggested that our developed vitrification and one-step dilution technique can be applied effectively on field trial for cloned animal production, which is even no longer in existence.