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http://dx.doi.org/10.5187/JAST.2008.50.5.641

Identification of Differentially Expressed Genes Between Somatic Cell Nuclear Transfer and Normal Placenta in Cattle  

Yu, Seong-Lan (Division of Animal Science and Resources, Research Center for transgenic Cloned Pigs, Chungnam National University)
Jeong, Hang-Jin (Division of Animal Science and Resources, Research Center for transgenic Cloned Pigs, Chungnam National University)
Sang, Byung-Chan (Division of Animal Science and Resources, Research Center for transgenic Cloned Pigs, Chungnam National University)
Ryoo, Seung-Heui (Livestock Experiment Institute, Government of Chungcheongnam-Do)
Jung, Kie-Chul (Division of Animal Science and Resources, Research Center for transgenic Cloned Pigs, Chungnam National University)
Yoon, Jong-Taek (Department of Animal Life Science, Hankyong National University)
Seong, Hwan-Hoo (Animal Biotechnology Division, National Institute of Animal Science)
Jin, Dong-Il (Division of Animal Science and Resources, Research Center for transgenic Cloned Pigs, Chungnam National University)
Lee, Jun-Heon (Division of Animal Science and Resources, Research Center for transgenic Cloned Pigs, Chungnam National University)
Publication Information
Journal of Animal Science and Technology / v.50, no.5, 2008 , pp. 641-648 More about this Journal
Abstract
There has been great success for making transgenic animals using somatic cell nuclear transfer(SCNT) up to this time. However, the success rates of the production of live transgenic animals are still very low. The current research has been carried out for delineation of differentially expressed genes between SCNT and normal placenta in cattle. In the present observations, high expression has been observed for CTSZ, LOC509426 and ELF1 genes in normal placenta. On the other hand, TIMP2, PAG1B, PAG-21, LOC782894, SERPINB6 and mKIAA2025 protein were highly expressed in SCNT placenta. Five genes, which were highly expressed in SCNT placenta, have been further investigated using semi-quantitative real-time PCR. The results were similar to that we observed using ACP. In the future, all genes affecting the SCNT and normal placenta have to be discovered and their networks will be fully investigated. The genes were identified in this study would be great help for identifying differential gene expressions in SCNT placenta.
Keywords
Somatic cell nuclear transfer(SCNT); Placenta; Annealing control primer(ACP); Differentially expressed genes(DEGs)
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