• Journal of the Society of Cosmetic Scientists of Korea
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• v.33 no.3
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• pp.189-196
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• 2007

In order to investigate the potential of very low molecular weight hyaluronic acid(oligo HA) as a cosmetic ingredient, we first measured its cytotoxicity in fibroblast, keratinocyte, and SIRC cell lines. For efficacy test, its moisturizing effect and penetration rate were evaluated in an artificial skin system and Caco-2 cells. Oligo HA did not show any cytotoxicity at a concentration of 300 ${\mu}g/mL$ in fibroblasts and 1,000 ${\mu}g/mL$ in keratinocytes but it showed weak proliferation. In vitro ocular test, oligo HA showed negligible cytotoxicity at the maximum concentrations used(2,000 ${\mu}g/mL$) in SIRC cells. In the test of the single and repeated cutaneous applications, oligo HA under occlusive patch did not provoke any cumulative irritation and sensitization. Oligo HA at a concentration of 0.01 % exhibited a more potent moisturizing effect than hyaluronic acid at a concentration of 0.01 %. In the permeability test using artificial skin and Caco-2 cell lines, hyaluronic acid(M.W. $1.1{\times}10^6$) was hardly observed in the down medium of the inserts. On the other hand, oligo HA(M.W. 5,000) was detected in the down medium up to 16.0 % at 6 h in Caco-2 cell culture and up to 90 % at 6 h in an artificial skin system. These results suggest that oligo HA could be useful as an active ingredient for cosmetics.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.31 no.1 s.49
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• pp.35-41
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• 2005

We investigated the effects of skin hydration and anti-irritation of exopolysaceharide (GF-glucan) produced from submerged culture of Grifola frondosa. The moisturizing effects and trans-epidermal water loss (TEWL) of GF-glucan in cosmetic products (O/W emulsion) were clinically studied. GF-glucan showed good skin hydration effects when compared with sodium hyaluronate which is a commonly using moisturizer. The skin protective effects of GF-glucan against sodium lauryl sulfate (SLS) were also found. Twice a day application of the CF-glucan to skin reduced local inflammation remarkably. Irritant reactions were assessed by various aspects of skin function, erythema (skin color reflectance), hydration (electrical capacitance) and TEWL. In the human skin test, 5-day treatment of the GF-glucan was found to reduce SLS-induced skin erythema and Improve bier regeneration. Taken all together, GF-glucan should be a very useful cosmetic ingredient, as a moisturizer, and a protecting agent for various skin irritations.

• Korean Journal of Food Science and Technology
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• v.33 no.1
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• pp.19-23
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• 2001

Physicochemical properties including viscosity, temperature and pH stability, color turbidity and moisture retention power of xylooligosaccharide(XO) as food material were investigated to apply to food process. The viscosity of 70 bx XO was 930 cP at $20^{\circ}C$ and 90 cP at $60^{\circ}C$, which was higher than that of sugar but lower than that of other oligosacchrides. XO remained stable after heat treatment for 1hr at $100^{\circ}C$ and pH 2.5 to 8.0 indicating excellent heat and pH stability. The pH of XO was measured $5.0{\pm}0.1$ and the color turbidvity of XO was very low at pH $2.5{\sim}4.0$. The color turbidity of XO increased as pH increased, but it was not high enough to affect food process. When heated with 1% glycine the color turbidity of XO was very low and changed little with pH changes. To measure moisture retention power XO was stored at $25^{\circ}C$ and 75% relative humidity for 71 day. XO retained more than 95% of the original weight; whereas sugar lost 28%. These results suggest that XO has useful physicochemical properties for various food process to improve the functionality of food.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.22 no.1
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• pp.60-69
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• 1996

3-APPA는 생체내 물질 Υ-amino butyric acid와 유사한 구조를 갖는 물질로서 human fibroblasts 3-dimensional culture system 등의 in vitro 시험에서 collagen 합성촉진 효과, 세포증식 효과를 나타내며, hairless mouse를 이용한 동물시험에서도 collagen 합성이 증가되는 것으로 보고된 바 있다. 또한 피부 안정성에서도 우수한 결과를 보여줌으로서 화장품 분야에서 새로운 노화억제 물질로서 주목을 받게 되었다. 본 시험에서는 180명의 피검자를 대상으로 3개월간의 임상시험을 통하여 새로운 노화억제 물질인 3-APPA의 효능을 기존의 GABA 유사물질, aminopropane sulfonic acid와 비교하였다. 본 시험은 double-blind, randomized, vehicle-controlled clinical study로서 180명의 피검자를 APSA군, 3-APPA군, vehicle군으로 분류한 후 3개월간 매일 저녁 취침 전, 안면에 시험제품을 사용하도록 하였으며, 사용 전, 사용 1주후, 2주후, 6주후, 12주후 5회 설문지를 통하여 피부상태를 조사하였다. 각 설문내용은 피부 건조도, 피부 탄력도, 피부 주름량, 피부 윤기도, 피부 거칠기 정도에 대한 5개 질문으로 구성된 효능에 관한 질문과 흡수정도, 끈적임, 매끄러움, 보습력, 보습지속성, 전체적인 사용감에 대한 6개 질문으로 구성된 사용성에 관한 것이었다. 각 질문에 대하여는 매우 좋음 1점, 좋은 2점, 보통임 3점, 나쁨 4점, 매우 나쁨 5점 사이에서 답할 수 있도록 하였다. 시험 결과 APSA군은 6주와 12주후 피부 건조도, 피부 거칠기 항목에서 사용전에 비하여 유의한 차이를 나타내었다. 3-APPA군은 6주후 피부 건조도, 피부 거칠기, 피부 탄력도 항목에서 시료사용전에 비해 유의한 차이를 나타내었으며 12주후에는 피부 건조도, 피부 탄력도, 피부 윤기도, 피부 거칠기 항목에서 유의한 차이를 나타내었다. 12주후 APSA군은 피부 주름, 피부 탄력도 항목에서 vehicle 사용 피검자군에 비하여 유의적인 차이를 나타내었으나 3-APPA군은 피부 탄력도, 피부 주름량, 피부 윤기 항목에서 vehicle군에 비하여 유의적인 차이를 나타내었다. 결론적으로 3-APPA와 APSA 사이에 유의적인 차이가 있지는 않았으나 3-APPA군은 APSA군에 비하여 6주, 12주에서 사용전과 유의한 차이를 나타내는 항목이 많았으며 12주에서 vehicle과 유의적인 차이를 나타내는 항목도 많으므로 3-APPA가 APSA 보다 광범위한 피부노화 억제 효과를 갖는 물질이라고 할 수 있다.

• Journal of the Korean Applied Science and Technology
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• v.32 no.2
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• pp.339-347
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• 2015

This study is to evaluate moisturizing effect and durability of UV A/B blocking activity with multiple (W/O/W) emulsion system. Most of the sun protective products come to be hot issue having both high SPF and long-lasting activity as using special products when is going out, mountain climbing and sports. Also, many consumers prefer the products which have the excellent waterproofing activity of sun care cosmetics as well as the non-sticky feeling that carried out the study of the sensorial science and texture preference. Therefore, development of the specific formulation using this multiple (W/O/W) emulsion technology, it has O/W type hydro skin feel having soft and moist texture when it is treated on the skin. Finally, this formulation is instantly changed to W/O type feel after adsorbed into the skin. The purpose of this study is to get high SPF lasting effect having high water resistance tactivity with high functional multiple (W/O/W) emulsion cream. We used major ingredients, UV-B absorbers were selected with ethylhexyl methoxycinnamate, isoamyl-p-methoxycinnamate, ethylhexylsalicylate, and octocrylene, UV-A absorbers were selected with butylmethoxydibenzoylmethane, bis-ethylhexyloxyphenol methoxy phenyltriazine. SPF effect of O/W type cream was 34.1. SPF effect of W/O/W type cream was 40.6 (increased about 19%). Water resistance effect after 4 hours, SPF effect of O/W type cream was 3.6 (quickly drop down). SPF effect of W/O/W type cream having 81.0 % waterproofing effect was 32.7 (decreased about SPF 7.9). Moisturizing effect of O/W cream at first was superior comparing multiple emulsion. But after 3 hours quickly was drop-down. Moisturizing effect of multiple emulsion was high comparing O/W type and other sun block creams after 4 hours was constantly maintaining water-content.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.22 no.2
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• pp.223-237
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• 2009

본 임상연구는 임상연구 모집 공고를 통하여 모집된 대상자 중 Atopic Dermatitis Research Group (2005) 한국인 아토피 피부염 진단기준에 따라 아토피 피부염으로 판정되고 현재 약물(한약, 양약)치료나 외용제 치료를 하고 있지 않은 만 5$\sim$50세의 아토피 피부염 환자를 대상으로 하였다. 피험자들을 이중맹검, 블록무작위배정하여 시험군과 대조군으로 나누어 6주간의 시험기간 동안 육안적 평가, 혈액학적 검사, 피부측정 장비를 통한 기기적 평가를 시험 전후에 실시하여, 시험군에 사용된 제품이 대조군 사용된 제품에 비하여 얼마나 효과적으로 아토피 피부염으로 손상된 피부 장벽을 회복하는지 관찰하였다. 본 임상시험을 통해 'Atomento solution & cream'이 손상된 피부장벽을 회복시키고 피부 보습력을 증가시켜 아토피 피부염에 효과적이었으며, 인체에 이상반응이나 부작용 없이 안전하게 사용될 수 있음을 확인하였다.

• Journal of the Korean Applied Science and Technology
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• v.39 no.1
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• pp.1-9
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• 2022

1, 2-Octanediol (OD) as a cosmetic additive has been used simultaneously as a preservative and humectant. To solve the skin problem by 1, 2-octanediol (OD), we have synthesized 1, 2-octanediol galactoside (OD-gal) using Escherichia coli β-galactosidase (β-gal). Meanwhile, the optimal amount of β-gal, OD concentration, pH, and temperature for OD-gal synthesis were 4.5 U/ml, 150 mM, 7.0, and 37℃, respectively. Under these conditions, 150 mM OD was converted into about 55.9 mM OD-gal during 24 hours, in which the conversion yield (mole basis) was about 37.2%. In addition, OD-gal of 67.4 mg could be purified from a 9 ml reaction mixture, in which the overall synthesis yield from OD to the purified OD-gal was about 34.1% (weight basis) and 16.2% (mole basis), respectively. We are expecting that these results will be helpful to develop a safer additive in the cosmetic industry as basic data.

• Journal of the Korean GEO-environmental Society
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• v.17 no.10
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• pp.45-53
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• 2016

At present, coffee consumption amount is annually on the rise in Korea, which results in about 0.27 million tons of coffee waste annually. They are mostly classified as food waste and deserted with moisture contained, being a serious environmental issue. Existing slope greening techniques, which are vegetation based soil-media hydroseeding measures, have problems such as lack of coherence, dryness or lack of organic matters. Therefore in order to assess usability of Spent Coffee Ground (SCG), medium-to long-term growth test was conducted under the indoor and outdoor conditions. According to the result of growth test, when SCG was mixed with existing base materials, moisturizing power increased and organic matter content was reinforced, promoting germination and growth in a medium term. Among others, under the condition when supply of water was discontinued, withering rate was lower than existing base materials and diverse phenomena resulting from lack of nutrition decreased. Therefore, SCG as a waste organic matter with abundant nitrogen has the characteristic of inhibiting early growth but was found to have a quality favorable to long-term growth resulting from water containing ability and the supply of organic mater and is judged to be a material to replace or complement existing base materials.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.3 s.47
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• pp.369-375
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• 2004

In this study, liquid crystalline (LC) is formed using Gemini surfactant (GS) type and moisturizing effect in vivo is measured. $3.0\;wt\%$ of sodium dicocoyl ethylene diamine (PEG)-15 sulfate (SCD-PEG-15S) is used as GS and $4.0\;wt\%$ of hydrogenated dimer acid esters (HDAE) as booster. For stabilizers, $2.0\;wt\%$ of behenyl alcohol (BA) and $1.0\;wt\%$ of Iyso-lecithin (LyL) are utilized. It is stabilized in pH from 4.0 to 10.5 and the best condition is in pH 6.5. The value of viscosity is $8,000\pm500$ cP. The most excellent particles are formed within the range of 4.0 to 15.5 um. Formed LC is observed around LC particles using polarization microscope. It is also observed that lamellar gel network structure is formed around LC particles. Moisturizing effect is improved by $13.6\%$ (P<0.05) compared to control when measured 30 min later after coating samples. After 1 h, moisturizing effect is improved by 1$12.6\%$ (P<0.05) than control while showing $28.3\%$ (P<0.05) of improvement after 4 h. These results may be caused from that manufactured LC forms lamellar structure so that it has better water-holding ability and absorbance of oil increases. This formula could be utilized by delivery system (DS) on skin so that this technology can be applied for manufactuing pharmaceuticals and cosmetics.

• The Korean Journal of Food And Nutrition
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• v.14 no.6
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• pp.585-590
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• 2001

The purpose of this study was to examine physical properties of the addition of Plantago powder on bread flour and dough fheology of white pan bread. Four levels(0.3, 0.6, 0.9 and 1.2%) of each Plantago powder with bread flour were tested for their effects in dough mixing using rapid visco analyzer, alveogram, farinogram and sensory test. Addition of Plantago powder(0.3, 0.6 and 0.9% ) showed almost same tendency on the initial pasting temperature but 1.2% increased it. Increment of Plantago powder showed increment of peak viscosity and final viscosity, L(extensibility) and G(swelling index) value in alveogram showed decrement with increasing Plantago powder. In farinogram the use of Plantago powder increased consistency and water absorption but decreased development time and stability. White pan bread using Plantago powder had higher value of Max. G and gardeness in rheometer than without using it. Sensory evaluation of white pan bread with 0.6% Plantago powder had the highest score.