• Title/Summary/Keyword: 병원성 미생물

Search Result 773, Processing Time 0.061 seconds

Analysis of Microbiological Contamination in Kimchi and Its Ingredients

  • Lee, Ji-Hyun;Ha, Ji-Hyoung;Lee, Hae-Won;Lee, Jae Yong;Hwang, Ye-Seul;Lee, Hee Min;Kim, Sung Hyun;Kim, Su-Ji
    • Journal of Food Hygiene and Safety
    • /
    • v.33 no.2
    • /
    • pp.94-101
    • /
    • 2018
  • Although Kimchi has health benefits, food poisoning associated with consumption of Kimchi has been frequently reported. Accordingly, microbiological properties of Kimchi (100 samples) and washing effects on microbial reduction against its ingredients (200 samples) were examined. Total aerobic bacteria, coliforms, Escherichia coli, Bacillus cereus, and Clostridium perfringens were quantified. In addition, B. cereus, Salmonella spp., Enterohemorrhagic E. coli, C. perfringens, Campylobacter jejuni/coli, Staphylococcus aureus, Vibrio parahaemolyticus, Listeria monocytogenes, and Yersinia enterocolitica were analysed qualitatively. Total aerobic bacteria count was approximately 1.4-9.0 log CFU/g, which were highest in ginger (8.8 log CFU/g), and Chonggak Kimchi (9.0 log CFU/g). The range of coliform counts detected in all raw materials was 0.5-7.3 log CFU/g and ginger showed the greatest number 7.3 log CFU/g among others. Contamination was decreased to 0.2-3.2, 0.3-2.7, and 1.0-3.9 log CFU/g for total aerobic bacteria, coliforms, and B. cereus, respectively, after washing. Minimising microbial contamination in Kimchi ingredients is necessary to ensure the safety of Kimchi. These results indicate that washing is a useful method to reduce bacterial contamination in Kimchi.

Effects of the microbiological properties and pathogenicity of Photobacterium damselae subsp. damselae under different culture conditions (배양 조건이 Photobacterium damselae subsp. damselae의 미생물학적 성상 및 병원성에 미치는 영향)

  • Kwon, Mun-Gyeong;Cho, Byoung-Youl;Park, Myeong-Ae
    • Journal of fish pathology
    • /
    • v.22 no.3
    • /
    • pp.239-251
    • /
    • 2009
  • The effects of microbiological properties and pathogenicity of Photobacterium damselae subsp. damselae were investigated under different culture conditions, temperature, pH, NaCl and iron concentration on culture media. Favorable conditions for bacterial growth were between 15-30${^{\circ}C}$, pH 5-9, 0-4% NaCl concentration and iron contents of over 10 mM, whereas the bacterial growth was inhibited under iron chelator existence. When P. damselae was cultured in iron-limited tryptic soy broth, total protein concentration of extracellular products, cytotoxic ability of ECPs on cell line, bacterial viability in flounder serum, phospholipase and siderophore activities of ECPs were significantly increased. On the other hand, the activities of P. damselae cultured under iron-added conditions were decreased. In this study, the iron-limited conditions were similar to the host in which iron concentration is low. During infection caused by P. damselae, the conditions could be related to the pathogenesis of the pathogen.

Environmental Microbial Assessment of Food Services at Elementary Schools in Western Gyeongnam Pyovince (서부 경남 지역 초등학교 급식소에서의 환경미생물 평가)

  • 박선자;하광수;심원보;박민경;정덕화
    • Journal of Food Hygiene and Safety
    • /
    • v.18 no.1
    • /
    • pp.14-24
    • /
    • 2003
  • Two aims of the present study were the evaluation of hygienic, microbial safety on food services of elementary schools in Western Gyeongnam province, and then, the construction of database for the SSOP (Sanitation Standard Operation Procedures) practice. A total of 98 samples were collected from drinking water, waterworks, kitchen utensils, kitchen equipments, employees and cooked foods. Total bacteria was counted and pathogenic bacteria such E. coli, salmonella, yersinia, vibrio parahaemolyticus and staphylococcus were identified based on the biochemical analysis. Following are the results: the number of bacteria showed from 1.0x10$^2$CFU/ mL to 1.0x10$^{6}$ CFU/mL in most samples, which the level exceeds normal range. Over 1.0x10$^{5}$ CFU/ml bacteria were observed from the kimchi in 4 places (B, C, D, E), because cooked food such as kimchi had not been heated. As a rule, the bacterium level in place B was higher than that in any of the other places. E-coli were isolated from kitchen knives (C, E) and Kimchi (E): staphylococci were isolated from drinking water (A), hands (D), refrigerator (E) and apron (E). But, salmonella, vibrio and yersinia were not detected in anywhere. In conclusion, the presence of bacteria and pathogenic agents in school food service was closely related to hygienic practice. For that reason, it is necessary to have more systematic and efficient management in order to enhance the food safety.

Cross Infectivity of Nuclear Polyhedrosis Viruses to the Common Armyworm, Pseudaletia separata (멸강나방에 대한 곤충핵다각체병바이러스의 교류감염성)

  • ;Okada Muneo
    • Korean journal of applied entomology
    • /
    • v.28 no.1
    • /
    • pp.10-15
    • /
    • 1989
  • This studies were carried out to selected high pathogenic nuclear polyhedrosis viruses(NPVs) against Pseudaletia(=Leucania) separata for the introduction of microbiol control of the insect NPV in Korea. Among 21 NPVs, Sesamia inferens and 4 P. separata NPV strains were highly pathogenic against P. separata when fed orchard grass leaves smeared virus suspension on the 2nd instar larvae. Three NPV strains (LsNPV-F, LsNPV-G, LsNPV-Y) were more susceptible to the younger instar than the older instar P. separata larvae when fed artificial diet mixed with the virus to the insect.

  • PDF

Isolation and Identification of Antimicrobial Compound from Amarantus lividus (참비름 추출물에서 항균성 물질의 분리 및 동정)

  • Oh, Young-Sook;Lee, Shin-Ho
    • Microbiology and Biotechnology Letters
    • /
    • v.33 no.2
    • /
    • pp.123-129
    • /
    • 2005
  • Isolation and identification of pathogens from slaughter and meat processing plant were investigated. Antimicrobial activity of Amaranthus lividus against isolated pathogens such as Aeromonas sobria, Escherichia coli, Escherichia coli O157, Listeria monocytogenes, Salmonella spp., and Staphylococcus aureus was investigated. Among the chloroform, ethyl acetate and buthanol fraction of amaranthus lividus showed inhibitory effect against Aeromonas sobria CLFM1 and Escherichia coli CLFM2. Antimicrobial substance in chloroform fraction was isolated by silica gel adsorption column chromatography, sephadex LH-20 column chromatography and silica gel partition column chromatography. The antimicrobial compound of amaranthus lividus was identified as diethyl phtalate by HPLC, GC-MS, H-NMR and C-NMR.

Microbiological Hazard Analysis for HACCP System Application to Hospitals Foodservice Operations (병원급식소의 HACCP 제도 적용을 위한 미생물학적 위해도 분석)

  • Lee, Byung-Doo;Kim, Jang-Ho;Kim, Jeong-Mok;Kim, Du-Woon;Rhee, Chong-Ouk;Eun, Jong-Bang
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.35 no.3
    • /
    • pp.383-387
    • /
    • 2006
  • Microbiological hazard analysis of foodservice facilities and utensils of 6 hospitals in the Honam region was evaluated. In the microaerosol evaluation, the microbial counts of dinning table, kitchen, and freezer were comparatively high, and it indicated the microbial contamination of these facilities should be effectively managed. In the microbiological hazard analysis evaluation of cooking utensils and appliances, the total plate counts of cutting boards, knife, and meal plates were comparatively high but did not reveal significance. The counts of coliforms, Staphylococcus aureus and Escherichia coli, were lower than the general limit of microbial contamination, and the microbiological safety of the cooking utensils and appliances were satisfactory. In the microbial safety evaluation of side dishes, microbial counts of heat-cooked foods were generally low and microbiological hazards of these side dishes were comparatively low.

Microbial Prevalence and Quality of Organic Farm Produce from Various Production Sites (생산지 수집 신선 유기농 농산물 미생물 분포도 분석)

  • Park, Won-Jung;Ryu, Hwa-Yeon;Lim, Ga-Yeon;Lee, Young-Duck;Park, Jong-Hyun
    • Korean Journal of Food Science and Technology
    • /
    • v.46 no.2
    • /
    • pp.262-267
    • /
    • 2014
  • To analyze the presence of microbes in organic farm produce, green chillies, lettuce, tomatoes, apples, pears, and rice were collected at 47 production sites with organic and conventional produce. Total average bacterial counts of 4.07 log CFU/g in organic green chillies, 3.71 log CFU/g in conventional green chillies, and 6.76- 6.90 log CFU/g in the both lettuce were detected. Mean bacterial counts of 4.48 log CFU/g and 2.84 log CFU/g were detected in organic and conventional pear produce, respectively. Differences in bacterial counts in tomatoes, apples, and rice in organic and conventional produce were less pronounced. Clostridium perfringens, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella spp., and Staphylococcus aureus were not detected in any produce sample. However, Bacillus cereus was detected with average counts of 1.04 log CFU/g in 11/47 (23%) conventional produce samples and 1.97 log CFU/g in 6/47 (13%) organic produce samples. Therefore, organic and conventional produce showed similar microbial prevalence patterns, and comparable safety in terms of pathogen contamination.

Study of 2,3,5-Triphenyltetrazolium Chloride for Detection of Pathogenic Microorganisms (2,3,5-Triphenyltetrazolium Chloride를 이용한 병원성 미생물 확인시험에 관한 연구)

  • Kang, Jung Wook;Bae, Jun Tae;Yeon, Jae Young;Kim, Young Ho;Kim, Jin Hwa;Lee, Geun Soo;Pyo, Hyeong Bae
    • Journal of the Society of Cosmetic Scientists of Korea
    • /
    • v.40 no.3
    • /
    • pp.307-311
    • /
    • 2014
  • 2,3,5-Triphenyltetrazolium chloride (TTC) is used as a redox indicator in culture media. It is colorless in the oxidized form and is reduced to formazan, an insoluble pigment, by dehydrogenases in actively growing microbial cells. The aim of this study was to assess by microbial test of the pathogenic microorganisms using TTC reduction. The pathogenic microorganisms were reduced in medium by dehydrogenase to produce insoluble red formazan. We observed that the optimization method of TTC allowed more than 12 h incubation in 0.04% concentration. Also, the growth of microorganisms with media was increased formazan production. We confirmed that microorganisms were quickly observed to grow colonies cultured red color without affecting the growth of microorganisms. It is suggested that the microbial test using TTC can provide better and quicker test method in cosmetics development.

Comparison of Antifungal Activity, Plant Growth Promoting Activity, and Mineral-Solubilizing Ability of Bacillus sp. Isolated from Rhizosphere Soil and Root (근권 토양과 뿌리로부터 분리된 Bacillus sp.의 항진균 활성, 식물 생장 촉진 활성 및 미네랄 가용화능 비교)

  • Kim, Hee Sook;Oh, Ka-Yoon;Lee, Song Min;Kim, Ji-Youn;Lee, Kwang Hui;Lee, Sang-Hyeon;Jang, Jeong Su
    • Microbiology and Biotechnology Letters
    • /
    • v.49 no.4
    • /
    • pp.576-586
    • /
    • 2021
  • The purpose of this study was to evaluate the antifungal activity, plant-growth-promoting activity, and mineral solubilization ability of 10 species of phytopathogenic fungi to select a Bacillus sp. from rhizosphere soils and roots that can be used as a microbial agent. The antifungal activity for phytopathogenic fungi varied based on the Bacillus sp. Among the selected strains, DDP4, DDP16, DDP148, SN56, and SN95 exhibited antifungal activity for nine or more species of phytopathogenic fungi. Regarding nitrogen-fixation ability, all Bacillus sp. showed similar levels of activity, and siderophore production ability was relatively high in ANG42 and DDP427. The indole-3-acetic acid production abilities were in the range of 1.83-67.91 ㎍/ml, with variations in activity based on the Bacillus sp. One strain with a high activity was selected from each species, and their mineral solubilization abilities were examined. Most Bacillus sp. could solubilize phosphoric acid and calcium carbonate, and DDP148 and SN56 could solubilize silicon and zinc, respectively. These results suggested that Bacillus sp. can be considered potential multi-purpose microbial agents for plant growth promotion and disease prevention.

Bioterrorism and Environmental Decontamination (생물테러와 환경소독)

  • Jeong, Woo-Dong;Mun, Sung-Min;Yoon, Je-Yong
    • Journal of Korean Society of Environmental Engineers
    • /
    • v.29 no.9
    • /
    • pp.1051-1059
    • /
    • 2007
  • Bioterrorism intends to cause mass casualties and social panic by means of malicious pathogenic microorganisms. Environmental decontamination becomes very important as a follow-up measure if that happens. Conventional methods for decontamination is that aqueous disinfectants are being sprayed for killing or not spreading microorganisms with the purpose of preventing infection. However, these procedures are not enough to perfectly sterilize space or surface inside of building, requiring additional measures such as surface disinfection or gas treatment methods. This article deals with the issues about the present decontamination procedures, global trends, in order to formulate suggestion for advanced environmental decontamination.