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http://dx.doi.org/10.15230/SCSK.2014.40.3.307

Study of 2,3,5-Triphenyltetrazolium Chloride for Detection of Pathogenic Microorganisms  

Kang, Jung Wook (R&D Center, Hanbul cosmetics Co. Ltd.)
Bae, Jun Tae (R&D Center, Hanbul cosmetics Co. Ltd.)
Yeon, Jae Young (R&D Center, Hanbul cosmetics Co. Ltd.)
Kim, Young Ho (R&D Center, Hanbul cosmetics Co. Ltd.)
Kim, Jin Hwa (R&D Center, Hanbul cosmetics Co. Ltd.)
Lee, Geun Soo (R&D Center, Hanbul cosmetics Co. Ltd.)
Pyo, Hyeong Bae (R&D Center, Hanbul cosmetics Co. Ltd.)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.40, no.3, 2014 , pp. 307-311 More about this Journal
Abstract
2,3,5-Triphenyltetrazolium chloride (TTC) is used as a redox indicator in culture media. It is colorless in the oxidized form and is reduced to formazan, an insoluble pigment, by dehydrogenases in actively growing microbial cells. The aim of this study was to assess by microbial test of the pathogenic microorganisms using TTC reduction. The pathogenic microorganisms were reduced in medium by dehydrogenase to produce insoluble red formazan. We observed that the optimization method of TTC allowed more than 12 h incubation in 0.04% concentration. Also, the growth of microorganisms with media was increased formazan production. We confirmed that microorganisms were quickly observed to grow colonies cultured red color without affecting the growth of microorganisms. It is suggested that the microbial test using TTC can provide better and quicker test method in cosmetics development.
Keywords
2,3,5-triphenyltetrazolium chloride; microorganism; microbial test; 1,3,5,-triphenylformazan;
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