• Journal of Animal Science and Technology
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• v.50 no.3
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• pp.407-416
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• 2008

본 연구는 CaCl2 용액의 주사량이 품종별 암사슴의 뿔 발생 유도에 미치는 영향을 구명하기 위하여 꽃사슴, 레드디어 및 엘크 암컷 각 6두에 대해 30% CaCl2 용액의 주사량을 1ml?, 1.5ml? 및 2ml?의 3처리구로 나누어 전두골 좌, 우 양쪽 골막에 3월과 7월 두 차례 주사한 후 사슴뿔 발생 유도율, 사슴뿔 생산량, 발육성적을 조사하였다. 품종별 암사슴의 뿔 발생은 꽃사슴이 1ml? 처리구의 한 마리 한쪽에서만 발생하였고, 레드디어는 2ml? 처리구의 한 마리 한쪽에서만 유도되어 꽃사슴과 레드디어의 뿔 발생 유도율은 8.3%였다. 엘크 암사슴은 1ml? 처리구에서는 뿔 발생이 일어나지 않았으며, 1.5ml?와 2ml? 처리구에서는 모두 뿔 발생이 유도되어 유도율은 67%였다. 뿔 생산량은 꽃사슴의 경우 길이 21cm?이고 무게 70g 이었으며, 레드디어는 길이 18.5cm?에 무게 83g의 뿔을 생산하였다. 엘크는 3월 1.5ml? 처리구의 길이가 평균 34±3cm?이었고 무게는 526~1,008g이며, 2ml? 처리구는 길이가 평균 39±8cm?, 무게는 693~1,379g의 범위에 있었다. 엘크의 2ml? 처리구는 7월에 주사한 것이 뿔 길이와 뿔 생산량에서 3월의 것보다 적었다. 암사슴 뿔의 발육은 엘크의 경우 주사 후 20일경에 길이 0.5~1cm? 정도의 돌기로 자랐고, 40일까지는 유발된 상처와 함께 1~3cm?정도의 완만한 성장을 보였다. 본격적인 성장은 처리 후 60일경이 지나야 일어났고, 120일경에 절각을 하였을 때 뿔 길이는 28~44cm?의 범위에 있었다. 생산된 암사슴 뿔을 세로로 절단하였을 때 전체 길이의 위쪽 70%는 골화가 되지 않은 상태였다. 암사슴의 뿔 발생 유도는 번식성적에는 전혀 영향을 미치지 않았다. 봄에 처리한 사슴의 재생 뿔 발생은 불규칙하게 일어났고, 재생 뿔 발생률은 평균 45%였으며, 주기가 진행될수록 길이와 생산량은 줄어드는 경향을 보였다. 결론적으로 30% CaCl2 용액의 주사량을 조절하여 처리하면 꽃사슴, 레드디어 및 엘크의 암컷에서 뿔 발생을 유도할 수가 있으며, 엘크 암사슴의 뿔 발생 유도를 위한 최적 주사량은 1.5~2ml? 범위였다.

• Journal of Animal Science and Technology
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• v.48 no.2
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• pp.255-268
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• 2006

Twenty four Hanwoo cull cows were assigned to 2 groups, control and melengerol acetate(MGA)+selenium supplement containing vitamin E(SeE), based on the parity(6.5±1.7 birth) and body weight (493.17±55.61kg), and the experiment was conducted to establish the reasonable fattening method of cull cows for 240 days. Average daily body gains during 240 days were 0.51kg and 0.63kg in control and MGA+SeE, respectively(P<0.10). DDMI/ADG of MGA+SeE group improved compared to control group(P<0.05). Therefore, supplementation of MGA+SeE in concentrates may accelerate both of the growth rate and feed efficiency in Hanwoo cull cows. MQI from MGA+SeE was more developed based on the larger rib-eye area and thinner backfat thickness in carcass than that from control. Marbling score for MGA+SeE tended to increase compared to control. Dietary Se supplementation significantly affected muscle Se concentration in longissimus dorsi meat of MGA+SeE group(P<0.05). Similar results to Se were obtained from α-Tocoperol concentration. During 7 days of simulated retail display, accumulations of thiobarbituric acid reactive substances(TBARS) concentration in beef was greater(P<0.05) in control than in supplemented cows. These results supported the hypothesis that supplementation of MGA+SeE improve the growth performance and carcass grade both by the growth stimulating effect of MGA+SeE and by preventing the oxidation of the longissimus dorsi muscle in Hanwoo cull cows.

• Korean Journal of Agricultural Science
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• v.4 no.1
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• pp.84-87
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• 1977

The distribution of number of ovulation between right and left ovary in superovulated mice were examined. 50 adult female mice of CF#1 strain were used in this study. Superovulation was induced by the subcutaneous injection of 5 IU PMS and followed 48 hours later by the subcutaneous injection of 5 IU HCG. Females a were killed at 24 hours after injection of HCG, and ova in the oviducts were counted under a microscope. The number of ova ovulated from the left and the right ovary were 603 and 594. The number of ova in both sides were found equal frequency by $X^2$ test method. Ovulation occurred more frequently in the left ovary than in the right in 24 females and with equal frequency in both ovaries in 3 females, while more frequently in the right ovary than in the left in 23 females. The possitive correlation was found between right and left ovary.

• Korean journal of applied entomology
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• v.50 no.4
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• pp.383-393
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• 2011

Because communication facilitates behaviors that are critical for survival and reproduction, it is central to the study of behavior and evolution. One of the most important and difficult issues with respect to communication has been the definition of communication itself. Broadly, it can be defined as an exchange of information from a signaler to a receiver. However, evolution of a signal is likely possible only under conditions in which both the signaler and receiver increase fitness from the exchange of information, often referred to as "true communication." The three primary sensory channels of communication used by animals are chemical, visual, and acoustic. Chemical signals are the oldest and most widespread method of communication. Visual and acoustic signals convey a great deal of information due to ease of modulation, flexibility of signal production, and fast transmission. The most widespread contexts in which animals communicate are sexual interaction and conflict resolution. Signals used for sexual interaction typically contain information about species identity and sexual attractiveness, whereas signals used for conflict resolution may contain information about resource holding potential. Other contexts under which animals communicate include territorial defense, parent-offspring interactions, social integration, sharing of environmental information, and auto-communication.

• Korean Journal of Agricultural Science
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• v.41 no.3
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• pp.221-228
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• 2014

The present study was conducted to investigate the effects of dietary supplementation of Codonopsis pilosula extract powder (CEP) on the reproductive performance in sows. A total of 20 pregnant sows were randomly assigned to two dietary treatments, which was given a corn and soybean meal-based diets containing 0 or 5 g/kg of the CEP from 40 days prepartum to weaning (25 days postpartum). The serum immunoglobulin G (IgG) concentration of sow (p<0.01) and piglets (p<0.05) was significantly increased by the feeding of CEP. And we also demonstrated that the coefficient of correlation between the colostrum and serum indicates a significant positive correlation on IgG. The stillbirth of sows and mortality rate during suckling periods were slightly lower in CEP feeding group than control, but there is no significant difference. In conclusion, our results suggest that the dietary supplementation of CEP increases the humoral immunity in both of sows and piglets, and it might contribute to improve the reproductive performance of sows. Further, additory studies are necessary on the immunomodulatory mechanism of CEP components.

• Korean Journal of Animal Reproduction
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• v.27 no.1
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• pp.69-75
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• 2003

The objective of this study is to improve the efficiency of embryo transfer in generation of transgenic mice using inbred mouse (C57BL/6J) embryos. The embryos of C57BL/6J and BCF1 mice were superovulated by the standard protocol. One-cell stage of embryos were microinjected and the resulted one-or two-cell were transferred into one-or two-side oviducts of foster mother, respectively. When one-cell stage of embryos were transferred into one-side oviduct of 0.75 d.p.c. foster mother, the results were not ideal because of showing pregnancy ratios of 68.8$\pm$7.83% for C57BL/6J and 48.3$\pm$14.22% for BCF1, and development ratios of pups vs transferred embryos of 11.9$\pm$5.51% for C57BL/6J and 10.5$\pm$8.03% fur BCF1 . However, when two-cell stage of embryos were transferred into two-side oviducts of 0.5 d.p.c. foster mother, we got better results of 94.4$\pm$9.64% and 100$\pm$0% pregnancy ratio, and 22.1$\pm$0.4% and 21.8$\pm$0.38% development ratio for C57BL/6J and BCF1, respectively. Therefore, transferring two-cell stage of C57BL/6J embryos into two-side oviducts of 0.5 d.p.c. foster mother may be competitive to the result in BCF1 embryos. Even if there are a lot of other factors affecting these results, we conclude from these experiments that transfer of two-cell embryos into two-side oviducts of 0.5 d.p.c. foster mother is a more efficient and safe method than others in generating transgenic mice using inbred mice embryos.

• Journal of Embryo Transfer
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• v.22 no.3
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• pp.195-198
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• 2007

This study was carried out to investigate the effects of hormones treated (progesterone, and combination of progesterone and estradiol) on the reproductive performance of dairy cattle. The intravaginal CIDR was inserted in the vagina of cow on day 14 (P-7, PE-7), 16 (P-5, PE-5) and 18 (P-3, PE-3) post-insemination to stimulate progesterone secretion with (PE) and without estradiol(P). The CIDR was removed on 7 (P-7, PE-7), 5 (P-5, PE-5) and 3 (P-3, PE-3) days after. The cows for control group were not treated with CIDR or hormones. Conception rate, the duration needed for conception after AI and the number of AI services needed for conception were measured. Conception rate in control, CIDR without estradiol (P-7, P-5 and P-3) and CIDR treated (PE-7, PE-5 and PE-3) cows were 15.8%, 40.0%, 31.5%, 28.6%, 62.5%, 50.0% and 0%, respectively. The days needed for conception after AI in control, P-7, P-5, p-3, PE-7, PE-5 and PE-3 were 64.9, 63.0, 59.1, 8.0, 0.0, 18.9 and 83.3 days, respectively. The days needed for conception in cows treated with CIDR (PE-7) has shown longer than control and PE-3 (p<0.05). The number of AI services needed for conception in control, CIDR (PE-7, PE-5 and PE-3) and CIDR without estradiol treated cows (P-7, P-5 and P-3) were 27, 2.3, 1.9, 1.3, 1.0, 1.7 and 2.8 times, respectively. The number of AI services needed for conception have shown significantly lower in PE-7 compared to control and PE-3.

• Journal of Embryo Transfer
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• v.22 no.2
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• pp.115-119
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• 2007

This study was carried out to investigate the effects of hormones (progesterone, and combination of progesterone and estradiol) on the reproductive performance of dairy cattle. The intravaginal CIDR was inserted in the vagina of cow on day 14 post-insemination to stimulate progesterone secretion with and without estradiol. The CIDR was removed on the day of next expected estrus. The cows for control group were not treated with CIDR or hormones. Conception rate, estrus interval, estrus intensity and serum progesterone were measured. Conception rates in control, CIDR without estradiol and CIDR treated cows were 15.4, 38.9 and 60%, respectively. Estrus interval in cows treated with CIDR was 26.5 days compared with 37.1 and 48.5 days in control and CIDR without estradiol treated cows. Estrus signs were more intense in cows treated with CIDR compared with control and CIDR without estradiol treated cows. Pregnant cows treated with CIDR showed higher serum progesterone concentration than pregnant and non-pregnant cows in control group. Particularly, serum progesterone was significantly higher in CIDR treated pregnant cows compared to non-pregnant cows at days 1, 2 and 6 of gestation. It may be concluded from present results that CIDR treatment is better than CIDR without estradiol to improve conception rate in dairy cattle.

• Korean Journal of Animal Reproduction
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• v.20 no.2
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• pp.207-214
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• 1996

To examine the developmental capacity of manipulated embryos after ultrarapid refreezing and thawing, mouse embryos were biopsied at 4-cell stage, frozen twice at 4-cell and morula stages, respectively, and then transferred to rec-ipients. Single blastomeres were biopsied from 4-cell embryos by a modified aspiration method. Biopsied 4-cell embryos were equilibrated into freezing medium at room temperature for 2.5 min, loaded into 40 $\mu$I of freezing medium in 0.25 ml plastic straw and then directly immersed into liqiud nitrogen. Freezing medium for 4-cell embryos consisted of 4.0 M ethylene glycol and O.25 M sucrose in dPBS supplemented with 6 mg/lm BSA. Morulae were frozen into freezing medium containing 5.0 M glycerol instead of ethylene glycol. Thawing was conducted by agitating each straw in 3TC water for 20 sec. The c content of each straw was expelled into 0.5 ml of dilution medium, which consisted of 0.25 M sucrose and 3 mg/ml BSA in dPBS. The thawed embryos were rehydrated in dilution medium for 10 min, washed 3 times with dPBS and then cultured in M16 medium at 37$^{\circ}C$, 5% CO$_2$ in air. Blastocysts that developed from frozen or refrozne biopsied embryos were transferred to recipients on Day 3 of pseudopregnancy, respectively. In vitro and in vivo developmental rates of the biopsied and intact 4~cell embryos after freezing and thawing were 78 (10l/130) and 25% (10/40), and 91 (114/125) and 30% (12/40), respectively. Although the rates of in vitro development of biopsied and intact embryos to blastocyst stage were significantly different after freezing and thawing (P

• Korean Journal of Animal Reproduction
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• v.25 no.4
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• pp.339-347
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• 2001

Sperm-mediated DNA transfer has a potential to markedly simplify techniques for the generation of transgenic animals. The exogenous DNA transfer by intracytoplasmic sperm injection (ICSI) procedure has been recently introduced in the production of transgenic animals. In this study, the developmental competence and tile expression rates of transgene were investigated after injection of spermatozoon or sperm head with enhanced green fluorescent protein (EGFP) gene into the mature porcine oocytes. The porcine oocytes were injected with intact sperm, membrane-disrupted sperm or sperm head. After injection. embryos were cultured in NCSU23 medium up to the blastocyst stage, and the developmental competence and expression rates were studied. The developmental rate (67.0%) of sperm injection group was higher than that (59.7%) of sperm head injection group, and the rates of EGFP expression were also significantly different between sperm injection and sperm head injection groups (42.1 vs 20.0%) (F＜0.05). In the porcine oocytes injected with sperm treated with different methods of membrane disruption, the removal of sperm membrane did not alter the developmental competence of embryos. The rate of blastocysts at 7 days after injection with intact and membrane disrupted sperm were 15.0 and 14.2%, respectively. The EGFP expression rates, 38.4% in embryos injected with frozen-thawed sperm was higher than that, 22.4% of embryos injected with the Triton X-100 treated sperm. Prior to injection, sperm were cultured in different EGFP gene concentrations from 0.Ol to 1ng/u${mu}ell$. However, no significant difference in developmental rates of embryos among different concentrations of EGFP gene were observed. The highest expression rate of EGFP gene, 37.4% was obtained from the embryos injected with spermatozoa treated with 0.1 ng/${mu}ell$ EGFP gene. These results suggested that exogenous DNA could be attached to the membrane disrupted sperm, and that these sperm could be used as a vector carrying foreign DNA into embryos.