Browse > Article

Studies on the Efficient Embryo Transfer Methods using Inbred Embryos in Generation of Transgenic Mice  

Wang A. G (Korea Research Institute of Bioscience and Biotechnology)
Kim, S. U. (Korea Research Institute of Bioscience and Biotechnology)
Y. H. Han (Korea Research Institute of Bioscience and Biotechnology)
Kim, S. K. (College of Vet. Med., Chungnam National University)
D. Y. Yu (Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Korean Journal of Animal Reproduction / v.27, no.1, 2003 , pp. 69-75 More about this Journal
Abstract
The objective of this study is to improve the efficiency of embryo transfer in generation of transgenic mice using inbred mouse (C57BL/6J) embryos. The embryos of C57BL/6J and BCF1 mice were superovulated by the standard protocol. One-cell stage of embryos were microinjected and the resulted one-or two-cell were transferred into one-or two-side oviducts of foster mother, respectively. When one-cell stage of embryos were transferred into one-side oviduct of 0.75 d.p.c. foster mother, the results were not ideal because of showing pregnancy ratios of 68.8$\pm$7.83% for C57BL/6J and 48.3$\pm$14.22% for BCF1, and development ratios of pups vs transferred embryos of 11.9$\pm$5.51% for C57BL/6J and 10.5$\pm$8.03% fur BCF1 . However, when two-cell stage of embryos were transferred into two-side oviducts of 0.5 d.p.c. foster mother, we got better results of 94.4$\pm$9.64% and 100$\pm$0% pregnancy ratio, and 22.1$\pm$0.4% and 21.8$\pm$0.38% development ratio for C57BL/6J and BCF1, respectively. Therefore, transferring two-cell stage of C57BL/6J embryos into two-side oviducts of 0.5 d.p.c. foster mother may be competitive to the result in BCF1 embryos. Even if there are a lot of other factors affecting these results, we conclude from these experiments that transfer of two-cell embryos into two-side oviducts of 0.5 d.p.c. foster mother is a more efficient and safe method than others in generating transgenic mice using inbred mice embryos.
Keywords
Inbred mouse; Embryo transfer; Superovulation;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Canseco, R. S., Sparks. A. E. T., Page, R. I., Russell, C. G., Johnson, J. L., Velander, W. H., Pearson, R. E., Drohan, W. N. and Gwazdauskas, F. C. 1994. Gene transfer efficiency during gestation and the influence of co-transfer of non-manipulated embryos on production of transgenic mice. Transgenic Res., 3:20-25
2 Duncan, D. B. 1955. Multiple range and multiple 'F' tests. Biometrics, 11: 1-42   DOI   ScienceOn
3 Mountz, J. D., Zhou, T. and Johnson, L. 1990. Production of transgenic mice and application to immunology and autoimmunity. Am. J. Med. Sci., 299:322-329
4 Polites, H. G. and Pinkert, C. A. 1994. DNA microinjection and transgenic animal production. In: Pinkert, C. V.(ed), Transgenic Animal Technology: A Laboratory Handbook. San. Diego, Academic. Press, pp.15-63
5 McLaren, A. and Michie, D. 1956. Studies in the transfer of fertilized mouse eggs to uterine foster-mothers I. Factors affecting the implantation and survival of native and transferred eggs. J. Exp. BioI., 33:394-416
6 Richa, J. 2001. Production of transgenic mice. Molecular Biotechnology, 17:261-268   DOI   PUBMED   ScienceOn
7 Pease, S., Schroeder, A. C. and Schmidt, G. H. 1989. Production of transgenic mice: acupuncture needle-facilitated embryo transfer to oviduct ampulla. Trends in Genetics, 5:293 (abstr.)
8 Smith, R. and McLaren, A. 1977. Factors affecting the time of formation of the mouse blastocoele. J. Embryol. Exp. Morphol., 41:79-92
9 Ingrid Van der Auwera and Thomas D'Hooghe. 2001. Superovulation of female mice delays embryonic and fetal development. Human Reproduction, 16(6):1237-1243
10 Heape, W. 1891. Preliminary note on the transplantation and growth of mammalian ova within a uterine foster mother. Proc. Royal. Soc., 48:457-458
11 Johnson, L. W., Moffatt, R. J., Bartol, F. F. and Pinkert, C. A. 1996. Optimization of embryo transfer protocols for mice. Theriogenology, 46:1267-1276   DOI   ScienceOn
12 Brinster, H. L., Chen, N. Y., Trumbauer, M. E., Yagle, M. K. and Palmiter, R. D. 1985. Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs, Proc. Natl. Acad. Sci., 82:4438-4442   DOI   ScienceOn
13 Hogan, B., Beddington, R., Costantini, F. and Lacy, E. 1994. Manipulating the Mouse Embryo. A Laboratory Manual. 2nd Edition. Cold Spring Harbor Laboratory Press, pp.129-135