• Korean Journal of Animal Reproduction
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• v.22 no.2
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• pp.137-143
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• 1998

The effects of continuous GH(hGH) secretion on the female reproduction was studies in adults female transgenic rats expressing the hGH gene with a mouse whey acid protein (mWAP) promotor. Two line of transgenic female rats carrying the mWAP/hGH gene were established and used in the study. One was characterized by relatively high levels of serum hGH (high line), and the other had relatively low levels (low line). 1. High line female rats had recurring, Pseudopregancy-like estrous cycles accompanied by increased serum progesterone level for 2 weeks after ovulation, and they were fertile. 2. In the rats, luteinization occurred spontaneously without cervical stimulation, probably due to high levels of serum hGH, which has prolactin (PRL)-like activity in the rat. 3. Low line female rats had recurring, regular 4-days estrous PRL surge following cervical stimulation were not, detected and PRL secretion was not induced by a dopamine antagonist. 4. The ovarian tissue in this line had a much higher number of corpora lutea and grew much heavier than in normal littermates, suggesting impairment of PRL induced structural luteolized. Su, pp.ession of PRL secretion in the low line rats was, at least in part, due to a marked decrease in the number of lactotrophs in the pituitary. The present study shows that the serum hGH level plays a crucial role in regulating luteal function in female transgenic rats expressing the hGH gene.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.116-116
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• 2003

명천산, 카마이시산, 나루산, 풍남산, 기장산, 울산산 미역(Undaria pinnatifida)의 성숙 포자체는 각각 2001년 5월에 명천 배양장에서 채묘하였다. 인공 채묘와 채묘의 주요방법은 손(1993)에 따랐다. 재배미역의 번식생태학과 생산량이 우수한 형질을 규명하기 위하여 2001년 12월부터 2002 4월까지 명천산, 카마이시산, 나루산, 풍남산, 기장산, 울산산 미역(Undaria pinnatifida)에 대해서 고흥군 명천 어장과 계도 어장에서 양식실험을 실시하였다. 형태적 형질에 있어서 명천, 계도 어장에서 전장은 3월까지 급격한 성장을 보였으나 4월 이후 성장이 점점 완만해 지면서 감소하였다. 품종간에도 전장의 성장도가 달리 나타났으며 그중 가장 큰 것은 가마이시산이었고 가장 작은 것은 울산산이었다. 경장은 3월까지 지속적인 증가를 보였으며 6품종중 1품종은 증감하였다. 품종별로 경장의 변화는 달리 나타났다. 각 어장별 품종별로 줄기길이는 대부분이 월별로 증가하다 4월부터 감소하였다. 그러나 명천산은 4월까지 증가 하였다. 성실엽장은 6품종 모두 4월까지 지속적인 증가를 보였으며 특히 풍남산과 가마이시산이 두 어장에서 크게 나왔으며, 울산산이 두 어장에서 가장 작게 나타났다. 명천 어장에서 6품종의 전장과 경장의 상관계수는 카마이시산이 가장 크게 나타났고, 울산산이 가장 작게 나타났다. 계도 어장에서도 카마이시산이 가장 크게 나타났으며 울산산이 가장 작에 나타났다. 전장과 줄기길이의 상관계수는 명천어장에서는 풍남산이 가장 크게 나타났고, 울산산이 가장 작게 나타났으며, 계도 어장에서는 카마이시산이 가장 크게 나타났고 울산산이 가장 작게 나타났다. 명천 어장에서 전장과 성실엽장의 상관관계를 보면 명천산의 상관 계수가 가장 크게 나타났으며 나루토산이 가장 작게 나타났다. 계도 어장에서는 카마이시산이 가장 크게 나루토산이 가장 작게 나타났다. 명천, 계도 어장에서 전장과 각 형질간 ANOVA 분석은 1%~5% 수준에서 품종별로 모두 유의성 있게 나타났다.

• Korean Journal of Medicinal Crop Science
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• v.10 no.3
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• pp.181-184
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• 2002

This study was conducted to select the optimum cultivar of wasabi in water condition. In three cultivar, Daruma, Simanesarai and Daihoichigo, the Daruma was superior to the others. Though total rhizome weight was similar to Daruma and Daihoichigo, rhizome of main stem of Daruma was bigger than that of Daihoichigo, specially distribute in $80{\sim}159g$ size. The allylisothiocyanate content was higher in rhizome than that of leaf, lateral rhizome and petiole. In three cultivar, the allylisothiocyanate content of rhizome, Daihoichigo, Daruma and Simanesairai was 0.3389, 0.6332, 0.3956(mg/g), respectively.

• Korean Journal of Animal Reproduction
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• v.18 no.2
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• pp.133-139
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• 1994

Two human lactoferrin expression vectors(pCChcLf and pCChcLf-1) were constructed using rat $\beta$-casein gene and human lactoferrin cDNA. The recombinant DNAs containing human lactoferrin cDNA were microinjected into the fertilized eggs of hybrid mice (BDF1 : C57BL$\times$DBA) and the DNA-injected eggs were treansferred into the oviducts of foster mothers. Genomic DNAs were isolated from the tails of mice born from the microinjected eggs and analyzed by Southern blot analysis. As a result, 5 and 9 transgenic mice with CChcLf and CChcLf-1 gene were produced, respectively. To determine tissue-specificity of transgene expression, Northern blot analysis was performed. Female transgenic mice were killed at day 10 of lactation and total RNAs from various tissues were isolated. Based on Northern blot analysis, it was shown that transgene was mainly expressed in the mammary glands of transgenic mice. In addition, the human lactoferrin in milk was detected by enzyme-linked immunosorbent assay. For this study, milk was obtained from the mammary glands of the transgenic mice at day 10 of lactation. In line #2 of CChcLf and line #7 of CChcLf-1 transgenic mice, human lactoferrin was secreted into the milk at concentration levels of 340ng/ml and 60ng/ml, respectively.

• Reproductive and Developmental Biology
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• v.34 no.1
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• pp.27-32
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• 2010

This study was conducted to estimate the effect of the breed, sire-breeds, farrowing year, farrowing season and parity on number of born alive (NBA), number of weaning (NW) and survival rates of weaning (SRW) in swine. The data were obtained from 46,704 litters of the Landrace, Yorkshire, Duroc and Cross breed farrowed from 1996 to 2005 at 142 GP are registered in Korean Animal Improvement Association (KAIA). There was highly significant effect of breed, sire breed, farrowing year, farrowing season and parity on NBA, NW, SRW (p<0.01). The result of this study could be available to genetic improvement of reproductive traits as a basic reference in Korean pig industry. To achieve the more effective improvement of reproductive traits, additional research such as genetic parameter evaluation should be performed.

• Korean Journal of Animal Reproduction
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• v.24 no.4
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• pp.347-356
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• 2000

포유동물의 난소 내에는 많은 수의 primordial follicles 과 preantral follicles 이 존재하고 있으며, 이것은 수정란을 체외생산하기 위한 잠재적인 난자의 공급원이 될 수 있다. 생쥐 preantral follicles 내에 존재하는 난자의 체외성장과 발달을 위하여 몇몇 배양체계가 개발이 되었으며, 적당한 배양조건에서 감수분열 능력이 없는 preantral follicles 내의 난자가 체외배양을 통하여 난자 직경이 증가하고 완전한 핵성숙을 하는 것으로 나타났다. 또한, 체외성장 및 성숙된 난자로부터 생쥐 산자의 성공적인 생산은 preantral follicles 내의 난자가 체외배양을 통해서도 완전한 발달능력을 얻을 수 있음을 입증하였다. 그렇지만, 생쥐 preantral follicle로부터 수정란의 체외생산능력은 매우 낮은 것으로 보고되고 있다. 한편 사람을 비롯한 돼지, 소와 같은 중ㆍ대가축의 경우에는 pre antral follicle의 체외배양을 통하여 감수분열능이 있는 단계의 난자로까지의 발달이 아직 보고가 되지 않고 있다. 따라서 지금까지의 연구결과들을 종합해 볼 때, preantral follicles의 체외배양조건을 개선하거나 새로운 배양체계를 개발에 대한 많은 연구가 요구되고 있다. 사람과 중ㆍ대가축의 preantral follicles의 체외배양체계의 확립은 우수한 형질을 가진 동물의 확장, 희귀동물 혹은 멸종위기 동물의 보존에 이용될 수 있을 것이며, 그리고 암치료를 위하여 화학적, 방사선 치료를 받아야 하는 여성에게서 향후에 불입치료를 위한 방법으로 활용될 수 있을 것으로 기대된다.

• Korean Journal of Animal Reproduction
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• v.25 no.3
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• pp.231-235
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• 2001

Gestation duration of cow is an important economical trait. Shortening the gestation duration decreases the production cost and increases the competitiveness of Hanwoo industry. Thus, this study was carried out to analyse the effect of expected factors on gestation duration and make an alternative idea for reduced gestation duration. Gestation duration was not affected by cow-parity, but significantly affected by farm, Al year and sire. Therefore, reducing the gestation duration of Hanwoo may require both providing optimum feeding environment and nutrition management for cow and selecting optimum sire for AI.

• Korean Journal of Animal Reproduction
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• v.20 no.4
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• pp.453-458
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• 1997

It is known that the incorporation of genes into transgenic mice is generally stable and is p passed on to succeeding generations in a Mendelian fashion. In this report, transgenic mice were set as a model to evaluate whether the transgenes are transmitted in a Mendelian principle in a successive generations and how they are tran s smitted into their offspring. A 3.0 kb linear DNA fragment, containing the MMTV LTR, bovine aSI casein cDNA and SV 40 splicing and polyadenylation site; was microinjected into fertilized mouse embryos. The tail DNAs of the resulting pups were subjected to dot and Southern hybridizations to screen transgenic founders. The DNAs of their offspring were anlyzed by PCR to confirm the transmission of the transgene from F0. Out of 72 live pups four pups (5.6%), 3 males and 1 female, were positive for the transgene. The rates of transmission from F0 into F1 were 33.3, 7.7, 0, and 62.5%. Those from F1 into F2 were 63.6, 5.9, and 68.8% and those from F2 into F3 were 85.7, and 88.2%. In this report, the transmission pattern of transgenes in transgenic mice into their offspring was demonstrated. It either follows or does not follow in a Mendelian fashion. Deletion or loss of the transgenes from F0 in some lines became apparant to the succeeding generations.

• Korean Journal of Animal Reproduction
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• v.26 no.3
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• pp.205-214
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• 2002

There are many methods to introduce exogenous DNA into embryo to produce transgenic animals. Exogenous gene can be integrated into oocyte by sperm vector. In this study, sperm was used as a vector for a transgene, which is encoding enhanced green fluorescent protein (EGFP). The objective of this study was to investigate the expression of exogenous gene in bovine embryos after injection of spermatozoa cocultured with EGFP DNA fragment. Spermatozoa were plunged into liquid nitrogen and thawed several times or shook in 0.2% Triton X-100 to remove sperm membrane followed by DTT treatment. The injected oocytes were co-cultured with vero cells in CR1aa, and expression of EGFP gene was observed under fluorescent microscope. Blastocyst formation rates of oocytes injected with sperm treated with DTT, DTT-freezing or DTT-Triton X-100 were 34.7, 39.4 and 31.9%, respectively. The rates of EGFP expression in oocytes injected with 54 ng DNA after DTT-treated, DTT-freezing and DTT-Triton X-100-treated sperm were 0, 19.1 and 13.9%. On the other hands, expression rate of oocytes injected with sperm cocultured with 13.5, 27 and 63.5 ng of EFGP DNA were 6.7, 9.0 and 5.1%, respectively. When intact sperm was mixed with 63.5 ng/${mu}ell$ EGFP DNA fragment, and then electroporated before injection, the expression rate of injected oocyte was 2%. Unexpectedly, electro-poration could not increase the expression rate. These results suggest that sperm can be used as a transgene vector, even if the efficiency was low (19.1%).

• Journal of Animal Science and Technology
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• v.54 no.5
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• pp.331-336
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• 2012

This study was conducted to estimate genetic parameters for ultrasound and carcass traits in Hanwoo. Heritabilities and genetic and phenotypic correlations were estimated for carcass and ultrasound measurements collected from Hanwoo cows (n=312) born at Hanwoo experiment station. Traits evaluated were eye muscle area (EMA), backfat thickness (BF), marbling score (MS) from carcass, and ultrasound eye muscle area (UEMA), ultrasound backfat (UBF), and ultrasound marbling score (UMS). Parameters were estimated using multi-trait animal models byderivative-free restricted maximum likelihood procedures. Estimated heritabilities for UBF, UEMA and UMS were 0.43, 0.23 and 0.32, while heritabilities for BF, EMA and MS were 0.33, 0.13 and 0.33 in fattened cows, respectively. Genetic correlations between ultrasound and carcass measurements were estimated to -0.19, -0.61, and -0.36 for UBF: UEMA, UBF: UMS, and UEMA: UMS in fattened cows, respectively. Phenotypic correlations between ultrasound and carcass measurements were 0.03, 0.13 and 0.26 for UBF: UEMA, UBF: UMS, and UEMA: UMS in fattened cows, respectively. As for the steer, genetic correlations between ultrasound and carcass measurements were 0.36, -0.80 and 0.27 for UBF: UEMA, UBF: UMS, and UEMA: UMS in steers, respectively. Phenotypic correlations between ultrasound and carcass measurements were 0.13, 0.07 and 0.41 for UBF: UEMA, UBF: UMS, and UEMA: UMS in steers, respectively. In conclusion, this finding would be very useful to implement into Hanwoo breeding program.