• Title/Summary/Keyword: 번식특성

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Genetic analysis of endangered species Crested Ibis (Nipponia nippon) microsatellite markers (Microsatellite Markers를 이용한 따오기의 유전적 특성 분석)

  • Kim, Da Hye;Kim, Yi Seul;Seo, Joo Hee;Kim, Sung Jin;Kong, Hong Sik
    • Korean Journal of Ornithology
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    • v.25 no.2
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    • pp.77-81
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    • 2018
  • The Korean Crested ibis Nipponia Nippon is an endangered species. A pair of Crested ibis was introduced from China in October 2008, and a successful program of artificial incubation of the species, and over 200 animals have been successfully bred through the restoration project up to 2017 at Upo ibis restoration center. We assessed genetic diversity and sex determination in the Korean Crested ibis. In total, 228 Crested ibis (115 females and 113 males) were identified. And genetic diversity measures, observed heterozygosity, expected heterozygosity, and polymorphic information content values were lower in 2017 than those in 2016. The inbreeding coefficient showed that the degree of ancestry increased in 2017. The decrease in polymorphism and increase in the degree of ancestry is thought to be due to inbreeding in such a small group. In this study provided important insight into protocols for genetic management of the breeding population of Korean Crested ibis in Korea and will help in extending the restoration program.

Characterization of Apoptosis in Porcine Primordial Germ Cells In Vitro (체외 돼지 원시 생식세포의 Apoptosis 특성 규명)

  • Lee, C.K.
    • Korean Journal of Animal Reproduction
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    • v.24 no.4
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    • pp.385-394
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    • 2000
  • When porcine primordial germ cells (PGCs) isolated from the genital ridge and placed in culture to establish EG cells, a large proportion of PGCs are lost during the early period of culture. To characterize the in vitro death of porcine PGCs, PGCs were cultured in suspension, and apoptosis analyzed using a fluorescent activated cell sorter-based DNA fragmentation assay. The results from flow cytometric analysis showed an increase in apoptosis in cultured cells. However, the cells isolated from the genital ridges are a mixture of PGCs and somatic cells. To detect apoptotic signals specific from porcine PGCs, quantitative TUNEL assay was performed at different time of culture (0 ∼ 24 h). The proportion of apoptotic porcine PGCs determined by double staining with alkaline phosphatase activity and in situ TUNEL assay increased as the time of culture progressed and continued at least 24 h. These results demonstrate that one of the causes of loss of porcine PGCs in vitro is apoptosis.

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Longitudinal Survey of Strix aluco During Breeding Season and Their Habitat in Korea -The First Reported Case Study (한국에서 올빼미 번식기 추적 조사 및 서식지 연구-첫 추적조사 사례)

  • Kim, Woo-Yuel;Choi, Won-Suk;Park, Jong-Chul;Sung, Ha-Cheol
    • Korean Journal of Environment and Ecology
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    • v.30 no.6
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    • pp.1067-1072
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    • 2016
  • In this study, we determined the ecological characteristics of Strix aluco, which is designated as an endangered category II species. Towards this, we identified the actual home range of Strix aluco that breed in Korea, and collected the data necessary for protecting, preserving and managing owl populations. This study was conducted from April 14 to May 20 of 2016 during the breeding season of Strix aluco as part of the 4th National Ecosystem Survey and in accordance with the Natural Environment Conservation Act. We captured and subsequently released a female owl with a total length of 423.5mm and a weight of 550g in Kyungbuk area, succeeded in tracking it for the first time in Korea and secured 6 points of direct observation and satellite tracking observation. The distance from the nest to the farthest point was 93m. As a result of the analysis of habitat points within a100m-radius during breeding period, it was established that their habitat consists of 34.2% of non-irrigated land, 30.6% of mixed forest land, 10.5% of orchard, and 9.4% of natural grassland. These findings are consistent with the previous estimate of home range studies. Further research based on follow-up studies on males or other individuals is required to develop a stronger basis for protection and conservation of endangered wildlife class II owls.

Habitat Environment of Eleutherococcus senticosus Max. at Mt. Deokyu (덕유산 가시오갈피 자생지의 생육환경)

  • 박문수;김영진;박호기;김선;김규성;장영선
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.6
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    • pp.710-717
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    • 1996
  • To obtain the basic informations on the farm cultivation of Eleutherococcus senticosus Max., field survey of the native habitat in Mt. Deokyu such as weather, soils, vegetation and growth was carried out. The habitat of E. senticosus is situated at 127$^{\circ}$ 45'E, 35$^{\circ}$ 52' N in Mt. Deokyu where the elev-tion ranges from 1,050 to 1,300 meters above the sea level. Mean annual temperature forecasted around the habitat was 5.8$^{\circ}C$, mean maximum temperature in August, 24.6$^{\circ}C$ ; mean minimum temperature in January, -13.5$^{\circ}C$ and relative humidity during the growth periods, over 95%. To divide the area according to climatic conditions was classified cool temperature and humid rain forest zone. In another view, it belonged to deciduous broad-leaf forest zone and soil texture was sandy loam with dark brown gravels. Acidity and P$_2$O$_{5}$ content of soil represented pH 5.2~5.6 and 10ppm, respectively. The growth of E. senticosus was poor under the low light intensity(relative 20% of full sun-light) at the growing place of the habitat and the propagation was conducted by root sucker. The natural vegetation was consisted of big trees, 3 species; shrubs, 8 species and herbs, 4 species.

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Comparison of Life Cycle of Several Korean Native Freshwater Cladocerans in Laboratory Culture Conditions (실내사육조건에서 한국산 물벼룩 종간 life cycle 비교)

  • Kim, Byung-Seok;Park, Yoen-Ki;Park, Kyung-Hun;Shin, Jin-Sup;Kim, Jin-Hwa;Yoon, Seong-Myeong;Ahn, Young-Joon
    • The Korean Journal of Pesticide Science
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    • v.10 no.3
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    • pp.196-200
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    • 2006
  • In this study we investigated fecundity and survivorship of four staple Korean freshwater cladocerans for their whole life to develop a new standard method used for ecological risk assessment of pesticide. The tested Korean freshwater cladocerans were Daphnia obtusa, Daphnia sp., Moina macrocopa and Simocephalus vetulus. In addition, standard test species, Daphnia magna endorsed formally by the major international organizations was tested together in order to compare with the reproductive characteristics of Korean cladocerans. A total of 358 young was produced by D. magna, whereas, 297 young was reduced by Daphnia sp. throughout the entire life. The average life span of Daphnia magna was 50 days much longer than any other Korean species. Nevertheless all of the Korean water flea tested showed good fecundity, produced over 60 young for 21 days as the validation criterion for reproduction toxicity test in Ecological Effects Test Guidelines published by USEPA. Especially Moina macrocopa produced their first brood in 4 days. Therefore 10 day reproduction test with Moina macrocopa may be an alternative good method to save cost and time to elucidating the effects of hazardous substances on the reproduction of aquatic invertebrates.

Effect of Elevated $CO_2$ Concentration and Temperature on the Phenology and Reproductive Ecological Characteristics of Bupleurum latissimum (Endangered plant) ($CO_2$ 농도와 온도 증가가 멸종위기식물 섬시호의 번식생태학적 특성에 미치는 영향)

  • Lee, Seung-Hyuk;Jeong, Jung-Kyu;Cho, Kyu-Tae;Jang, Rae-Ha;Han, Young-Sub;You, Young-Han
    • Korean Journal of Ecology and Environment
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    • v.46 no.3
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    • pp.380-387
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    • 2013
  • The objective of this study is to investigate the effect of elevated $CO_2$ and temperature on the phenology and reproductive ecological characteristics of Bupleurum latissimum. We measured characteristics of 4-year-old B. latissimum grown under control (ambient $CO_2$+ambient temperature, 340~370 ppm) and treatment (elevated $CO_2$+elevated temperature, 690~770 ppm) conditions in a glass house. The period of flowering, fruit appearance and fruit maturing was earlier by 4, 1, 2 days, respectively, under elevated $CO_2$ and temperature. The comparative weight of a single ripe fruit, all ripe fruits and total ripe and unripe fruits was higher per control than treated plants. However the other characteristics, such as no. of peduncle, matured fruits, total fruits per plant and fruit setting rate per plant of B. latissimum were not significantly affected by elevated $CO_2$ and temperature. The germination rate of B. latissimum was higher in control than in treatment plants. These results showed that the phenology of B. latissimuin might be well suited to an earlier growing season, and the response of reproductive characteristics of B. latissimuin is negatively influenced by global warming.

Studies on the Nest site Selection and Characteristics of Habitat use of Long-tailed Tits Aegithalos caudatus in Korea (한국에서 번식하는 오목눈이의 둥지장소 선택과 서식지 이용 특성)

  • Baek, Chung-yeol;Lee, Jin-hee;No, Sun-ho;Jang, Kyung-Hee;Cho, Sam-rae
    • Korean Journal of Environment and Ecology
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    • v.30 no.5
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    • pp.829-836
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    • 2016
  • This study was carried out on Kongju National University and its neighboring area, Mt. Gyeryong national park and Mt. Worak national park in the breeding season of long-tailed tits Aegithalos caudaatus from 2008 to 2012. The result of the inquiry, the nests were found in 15 species of trees, and this result shows me it's not choosy about them. But after having classified them according to the features and analyzed them, long-tailed tits preferred evergreen needleleaf tree and deciduous broad-leaf tree and deciduous bush, especially evergreen needleleaf tree. The concealment rate of long-tailed tits' nests was $11.61{\pm}4.79%]\;4.38{\pm}2.19%$(n=29) in Kongju National University and it's neighboring area, $6.06{\pm}2.31%$(n=43) in Mt. Gyeryong national park and $4.38{\pm}2.19%$(n=32) in Mt. Worak national park. By regional groups, the concealment rates of neighboring nests were almost same, but there are no differences among the areas. The average of the color codes was $26.52{\pm}16.14$(n=32) that those of the nest subtract from those of surroundings of the nest. The nests were procryptic according to their surroundings, and if their nest in a tree were evergreen needleleaf trees, they were made of moss. while if the trees of nest in a tree were yellow because of fungus, made of dried moss, they were yellow, too.

Implementation of unmanned cow estrus detection system for improving impregnation rate (번식률 향상을 위한 무인 발정발현 관찰시스템 구현)

  • Kim, Suc-June;Jee, Sun-Ho;Cho, Hyun-Chan;Kim, Chun-Su;Kim, Hyeon-Shup
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.16 no.9
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    • pp.6236-6246
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    • 2015
  • In the paper, we reduce non-pregnant conditions and improve impregnation rate by unmanned estrus detection and decide proper time for artificial insemination. It is too hard to detect estrus only by using activities, we develop unmanned estrus detection system that consist of RF activity sensors, cow management program and estrus detection algorithm that uses information of activities and breeding. We verify performance by experiments in four similar scale stockbreeding farmhouse. Each stockbreeding farmhouse breeds 87, 81, 93, 82 cows and expected estrus cows are 14, 19, 15, 17. In expected estrus cow, we fail in weak estrus detection - 3, 2, 1, 3 cows, but detect successfully normal estrus - 11, 17, 14, 14 cows. After artificial insemination, 10, 17, 13, 14 cows became pregnant successfully confirming that proposed unmanned estrus detection system is effective for deciding proper time for artificial insemination in normal estrus.

Changes in Leaf and Reproductive Traits of Mountain Ash (Sorbus alnifolia) as Urban Flourisher in the Seoul Metropolitan, South Korea (한국 서울 식생의 번성자로서 팥배나무의 형질 변화 양상)

  • Jung, Song-Hie;Cho, Yong-Chan;Lee, Chang-Seok
    • Korean Journal of Environment and Ecology
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    • v.35 no.6
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    • pp.644-658
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    • 2021
  • Understanding the functional traits of dominant species in urban ecosystems provides insight into species' trait adaptation and ecosystem function in response to fragmented and isolated urban vegetation and reduced biological interactions. This study compared means and variances of environmental factors (geographic, meteorological, and soil attributes) and 4 leaf traits (leaf area, specific leaf area, leaf dry mass content, and leaf shape index) and 7 reproductive traits (fruit width, fruit length, fruit shape, fruit dry weight, fruit dry matter content, seed weight, and seed ratio) measured of 40 Sorbus alnifolia individuals in four mountainous areas south of Seoul downtown, South Korea. We then performed the multivariate analysis of trait combinations. While the measured environmental factors indicated the individuality of the survey sites, the urban vegetation was drier and had a longer growth period. The leaves of S. alnifolia in the urban areas were smaller and heavier, and the fruits produced longer and lighter seeds, showing the traits affected by long urbanization. The study confirmed changes in the growth and reproduction mechanism of the S. alnifolia population under the urban environment, indicating reduced biological interaction due to vegetation fragmentation and isolation. This study provides limited but distinct ecological information about the function and persistence of key species in cities with a reduced scale of biological interactions and many negative environmental factors such as air pollution.

A Study on the Evaluation and Maintenance for Alternative Habitats of the Narrow-mouth Frog (Kaloula borealis) - A Case Study on the Alternative Habitats of Kaloula borealis at the University of Seoul - (맹꽁이 대체서식지 조성 평가 및 유지관리 방안 연구 - 서울시립대학교 맹꽁이 대체서식지를 사례로 -)

  • Park, Seok-Cheol;Han, Bong-Ho;Park, Min-Jin
    • Journal of the Korean Institute of Landscape Architecture
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    • v.47 no.1
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    • pp.76-87
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    • 2019
  • The purpose of this study was to evaluate the performance of and to derive future maintenance-management measures of the constructed alternative habitat for the Kaloula borealis at the University of Seoul, examining the period between 2015-2017. The research was constructed in 2014 and in a $191m^2$ area. The performance evaluation was divided into maintaining the habitat of the target species, maintaining the population and reproduction rates of the target species, maintaining the habitat of the wild species, the resilience of natural ecosystems, and the harmony with the surrounding environment. In terms of maintaining the habitat of the target species, soil collected from the existing habitat of the Kaloula borealis and was the depth was increased to 30cm in the alternative habitat. An artificial water supply was required every year during the supporting the spawning and hatching of other amphibians along with the Kaloula borealis. The sources of water of the alternative habitat were both rain and tap water, as it cannot be maintained naturally. Additionally, the Kaloula borealis thrived because it inhabited the research site and the average temperature was $26.2^{\circ}C$ from April-June, which is when the Kaloula borealis spawns. In terms of maintaining the population and reproduction rates of the Kaloula borealis, they were evaluated to have stable rates of reproduction. In terms of maintaining the habitat of the wild species, studies on vegetation and the structure of the characteristics of prey or predators will be needed. Also, alien species, such as Humulus japonicus and Bidens frondosa needed to be removed to maintain the wetland ecosystem of the wild species. In the assessment of the resilience of the natural ecosystems, the mud was monitored, noting the changes in the depth of water, with steps taken to reduce the leakage of water. The mud collected from the Haneul Pond wetland, which is located around the research site was piled up. Also, partial mowing management and the inducement of a natural vegetation colony was required for vegetation management. It was also necessary to create porous spaces, such as old trees and tree branches to create a habitat with hiding places and feeding and spawning places for small organisms. In terms of the harmony with the surrounding environment, the following threat factors needed to be managed: amphibian roadkill by vehicles and pedestrians and artificial draining due to nearby user access. Based on the monitoring results, alternative habitat management measures presented the promoting various waterside structures, in which amphibians can spawn and hide in, managing the water environment consistently, managing the vegetation, focused on the habitat of the wild species, and managing the surrounding environment for the habitat. The creation of an alternative habitat should be managed through monitoring, reflecting the characteristics of the changes in the site. Also continuing efforts are also needed to improve the habitat of the target species.