• Korean Journal of Food Science and Technology
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• v.40 no.6
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• pp.669-673
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• 2008

For the development of a biological control method against B. cereus in cheonggukjang, 20 Bacillus spp. were isolated from the naturally fermented baektae and heuktae cheonggukjang, identified by using 16S rDNA sequences. Among the isolated strains, Bacillus sp. SC-8 was selected using the B. cereus lawn cell assay as an antagonistic microorganism against B. cereus. The culture medium of Bacillus sp. SC-8 after 24 hr of incubation at $37^{\circ}C$ also evidenced a high level of antagonistic activity. In cheonggukjang fermented with the mixed culture of Bacillus sp. SC-8 and B. cereus, antagonistic effect against B. cereus was maintained during the fermentation of cheonggukjang, while its effect was reduced during storage at $4^{\circ}C$ due to the decrement of cell population of Bacillus sp. SC-8. In Bacillus sp. SC-15, which was utilized a control, antagonistic activity against B. cereus was not demonstrated on the lawn cell plate assay and culture medium, but its effects were detected in cheonggukjang. Therefore, the production of antagonistic substances of Bacillus spp. depends on the fermentative environment.

• Korean Journal of Food Science and Technology
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• v.40 no.3
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• pp.354-359
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• 2008

A survey for zearalenone contamination was conducted on 27 soy bean samples, 27 red bean samples, 16 black bean samples, 19 seoritae samples, 14 seomoktae samples, for a total of 127 commercial Korean samples. Zearalenone was quantified by the immunoaffinity column clean-up method with high performance liquid chromatography-fluorescence detection (HPLC-FLD), and was confirmed by liquid chromatography tandem mass spectrometry(LC-MS/MS). The limits of detection and quantification were $2.0{\mu}g/kg$ and $6.0{\mu}g/kg$, respectively. The recovery in the beans ranged from 82.2 to 98.4%. According to HPLC-FLD, zearalenone was detected in 13 samples (10.2% incidence), including 1 soybean and 12 red bean samples. The zearalenone contamination levels were in the range of 8.01${\sim}38.98{\mu}g/kg$. Finally, LC-MS/MS analysis was conducted in the contaminated samples to verify the results of HPLC-FLD. The LC-MS/MS results confirmed the presence of zearalenone in all 13 samples. The contamination level was lower than that of EU, which is below $100{\mu}g/kg$ for raw grains.

• Journal of Mushroom
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• v.11 no.4
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• pp.208-213
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• 2013

The contents of raw materials which are components of mixed substrate for mushroom cultivation were analyzed to optimize the composition. The pure protein(amino acid) level of soybean meal was the highest, 44.02% followed by those of soybean curd residue(31.5%) and cotton seeds meal(30.6%). The non protein nitrogen(NPN) contents in crude protein of main nitrogen materials were 2.4% for soybean meal and 5.6% for dried soybean curd residue, while those of wheat bran and rice bran used as the carbon source were relatively higher, 17.6% compared to that of nitrogen supplying media. Crude protein content per price was 6.0 for rapeseed meal, indicating that it is high crude protein content per price. Nitrogen-free extract(NFE) considering as an ingredient for mycelial growth were high in alphacorn(72.9%) and wheat bran B(57.2%). Acid detergent fiber(ADF) was high in corncob, 51.88%, its use for cultivation of brown rot fungi including Lentinus lepideus should pay attention because the fungi lack complete lignin degradation activity.

• Journal of Sasang Constitutional Medicine
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• v.10 no.1
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• pp.235-252
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• 1998

1. Background and Purpose : I intended to be helpful to understand constitutional symptoms of a disease and an application of the prescription through a clinical study of Taeumin Chongsim Yonja Tang. 2. Methods : I studied 125 patients(62 men and 63 women) treated with Taeumin Chongsim Yonja Tang in constitutional clinic of Dongguk Pundang Oriental Hospital. And then I investigated the characteristics of Chongsim Yonja Tang treated patients, remedial values and side effects. 3. Results : Taeumin Chongsim Yonja Tang was used in all age groups both men and women. There were 60 kinds of chief complaints including numbness of hands and feet, headache and fatigue etc. The distributions of remedial values were slightly improved(60.8%), much improved(15.2%), no improved(24.0%) and there was no aggravated patient. Using the prescription, mostly I prescribed only Taeumin Chongsim Yonja Tang without any changing, and added 2-3 herbs to the prescription according to the symptoms of a disease. Taeumin Chongsim Yonja Tang was affected in remedial values by the period of sufferring from illness, treatment and medication. Side effects were diarrhea, indigestion and so on.

• Journal of the Korean Society of Food Science and Nutrition
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• v.15 no.3
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• pp.263-275
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• 1986

Gulbi were processed by salting Yellow corvenia (Pseudosciaena manchurica) with in three ways: the dry salting method with bar salt, the dry salting method with purified salt or with the abdominal brine injection method with purified salt. The half of the sample was dried by the control system of temperature and humidity; the other part was dried by the natural condition. The moisture content of fresh Yellow corvenia muscle and eggs were 76.8%, and 68.2% while those of dried samples decreased to 57.7% and 45.3%, respectively. The total nitrogen content of fresh muscle and eggs were 11.0g% and 7.6g%, respectively (dry weight basis), which decreased slightly during salting and showed no significant changes during drying prosess. The protein nitrogen content of fresh muscle and eggs were 10.2g% and 7.5g%, which decreased during Gulbi processing. On the other hand, the nonprotein nitrogen content of both muscle and eggs increased. The content of free amino acids of fresh muscle and eggs were 508.8mg/100g and 1,110.6mg/100g, which increased to between 5.3 and 2.7 times, respectively after 25 days of drying. The composition patterns of free amino acids in muscle and eggs were similar to each other. The four amino acids - Ala, Glu, Lys and Leu - were most abundant in both fresh and dried samples. These amino acids are known as taste and flavour constituents.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.23 no.1
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• pp.61-71
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• 2010

The attributes related to the health condition of RC deck slabs are analyzed to help us identify and rate the safety level of the bridges in this study. According to the related reports the state assessment for the outward aspects of bridges is the important and critical part for rating the overall structural safety. In this respect, the careful identification for the various state attributes make the field inspection and structural diagnosis very effective. This study analyzes the influence of the state attributes on evaluation classes and the relationship of them by the inductive reasoning, which raise the understanding and performance for evaluation work, and support the logical approach for the state assessment. ID3 algorithm applied to the case set which is constructed from the field reports indicates the main attributes and the precedence governing the assessment, and derives the decision hierarchy for the state assessment.

• Journal of Sasang Constitutional Medicine
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• v.10 no.2
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• pp.455-471
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• 1998

Background and Purpose : We intended to be helpful to understand constitutional symptoms of a disease and an application of the prescription through a clinical study of Soyangin Yangkyuksanwhatang. Methods : We studied 65 patients(40 men and 25 women) treated with Soyangin Yangkyuksanwhatang in constitutional clinic of Dongguk Pundang Oriental Hospital. And then we investigated the characteristics of Yangkyuksanwhatang treated patients, remedial values, onset of action and side effects. Results: Soyangin Yangkyuksanwhatang was used in all age groups both men and women. There were 37 kinds of chief complaints including chest discomfort, numbness of hands and feet, motor disturbance and skin disease etc. The distributions of remedial values were slightly improved(58.5%), much improved(12.3%), no improved(29.3%) and there was no aggravated patient. Using the prescription, mostly I prescribed only Soyangin Yangkyuksanwhatang without any changing, and added some herbs to the prescription according to the symptoms of a disease. Soyangin Yangkyuksanwhatang was affected in remedial values by the addition and subtraction of herbs. The onset of action in young age group was shorter than old age group. Side effects were palpitation, abdominal pain and indigestion etc.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.3
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• pp.521-526
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• 2002

A number of experimental and epidemiological studies have implicated that antiestrogenic effects of estrogen-like compounds in legumes and plant seeds are responsible for lowering breast cancer risk in human. However, few studies have been conducted to illustrate the possible chemopreventive effects of Korean traditional food materials. This study was performed to determine the cytotoxic and apoptotic effects of yellow soybeans, black soybeans and brown rice extracts on hormone-dependent and hormone-independent human breast cancer cells. Methanol-or acetone-soluble fractions of soybeans or brown rice were incubated with hormone-dependent cells (MCF-7) or hormone-independent cells (MDA-MB-231). Cell cytotoxicity was measured by MTT assay at 24, 48 and 72 hrs of incubation. Apoptotic effects of these extracts toward breast cancer cells were also determined at 48 hrs of incubation by measuring DNA fragmentation. Results indicated that the acetone-soluble fraction of brown rice exerted strongest cytotoxic effect on MCF-7 ceIls, although other fractions also reduced the number of viable MCF-7 cells after 48 hrs of incubation. Both acetone and methanol soluble fractions of all samples exerted a significant cytotoxicity towards MDA-MB-231 cells after 24 hrs of incubation, and acetone and methanol soluble fractions of brown rice were especially effective in these cells. At 48 hrs of incubation, methanol fractions of all three samples induced apopotosis of MDA-MB-231 cells. These results indicate methaol or acetone soluble fractions of yellow soybeans, black soybeans and brown rice induce cytotoxicity in both hormone-dependent and hormone-independent breast cancer cells. Therefore, possible mechanisms of cell cytotoxicity do not necessarily include antiestrogenic effects of soybean or brown rice extract. A possible anticarcinogenic effect of brown rice methanol-soluble fraction may mediated through their apoptotic effect. Further studies are requried to elucidate responsible compounds and mechanisms involved in observed anticarcinogenesis.

• Applied Biological Chemistry
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• v.7
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• pp.79-84
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• 1966

1. In order to elucidate the differences between varieties of Korean soybean, forty-nine subjects were examined for their chemical constituents. Their proportions varied considerably with the differences in variety. The moisture varied from 10.93% to 12.57%(aver. 11.95%), protein; 33.79% to 47.00%(aver. 39.8%) crude fat; from 16.32% to 24.79%(aver. 19.2%), crude fiber; from 3.03% to 5.57%(aver. 3.98%), ash; from 3.95% to 5.23%(aver. 4.6%) and nitrogen free-extracts (earbohydrates); from 16.58% to 26,81% (aver. 20.57%) Chemical composition of soybean was affected by the varieties as well as their localities of growth. 2. Of the total protein, 33.26% based on the soybean meal was soluble protein and 1.94% insoluble protein in average.

• The Korean Journal of Food And Nutrition
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• v.30 no.5
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• pp.1119-1126
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• 2017

Quality characteristics of commercially available soybean and mung-bean flours in Korea were evaluated relative to product information such as bean content, price, chromaticity, proximate composition, water binding capacity, swelling power and solubility. Origin of raw materials was 23 in domestic products and 19 in imported products. The price of the product was 3.48 times more for raw soybean flour, 3.34 times more for fried soybean flour, and 3.47 times more for mung bean flour compared to imported soybean. In the domestic products, the lightness of raw soybean flour was higher than roasted soybean flour. The redness and yellowness of roasted soybean flour were higher than raw soybean flour. Moisture content of raw soybean flour was higher than roasted soybean flour, and crude fat, crude protein and carbohydrate content were higher in imported soybean flour. Ash and crude protein contents of mung bean were slightly higher than imported products. Water binding capacity of roasted soybean flour in domestic products was higher than raw soy flour, and there was no significant difference in solubility and swelling power. Correlation between quality characteristics of commercially available soybean and mung-bean flours products was found to be highly significant among measured items, except for solubility.