• 미생물학회지
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• 제41권4호
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• pp.269-274
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• 2005

동물용 생 바이러스 백신 내에 mycoplasma를 검출하기 위해 polymerase chain reaction (PCR)기법과 2가지의 상품화된 PCR 검출킷트를 평가하였다. PCR기법은 시험에 사용된 모든 mycoplasma를 특이적으로 검출할 수 있었으나, 2가지의 상품화된 PCR 검출킷트는 일부의 mycoplasma를 검출하지 못하였다. 또한, PCR기법의 검출 특이도는 조류 유래 mycoplasma에 속한 4주의 표준주 및 7주의 야외분리주를 모두 검출할 수 있었다. PCR기법의 민감도는 9 CFR Mycoplasma액체배지에서 배양한 Mycoplasma 속균 및 Acholeplasma속균에 대해 $1\~100$ colony forming units/ml까지 검출할 수 있었다. 동물용생 바이러스 백신에 대해 PCR기법의 적용가능성을 평가하기 위해, 돼지 전염성위장염 및 로타바이러스 흔합백신과 개 파보바이러스 백신내에 A. laidlawii를 인공적으로 접종한 후, PCR기법의 민감도를 조사하였을 때 배양액을 이용한 검출한계와 유사하였다. 본 연구에서 사용된 PCR 기법은 동물용 생 바이러스 백신내의 mycoplasma를 신속하고 민감하게 검출할 수 있을 것으로 판단되었다.

• Parasites, Hosts and Diseases
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• 제36권3호
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• pp.203-206
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• 1998

닭에 있어서 닭와포자충 감염이 다른 중요 가금 질병에 대한 체액성 면역억제 현상을 규명하기 위해 2일령의 보통 병아리에 $2{\;}{\times}{\;}10^6$의 닭와포자충 난포낭을 한번에 경구 투여한 다음 4일과 14 일 두 번에 걸쳐 닭전염성기관지염 생독백신을 접종하여 1주 간격으로 혈구응집억제반응으로 항체 가를 측정하였다 감염 및 비감염군 모두 평균 항체가는 점점 낮아져 2차 예방접종 후 2주에 최저 에 이른 다음 점점 높아져 일정한 수준을 유지하였다. 일반적으로 평균 항체가는 비감염군에 비하 여 감염군이 의의있게 낮았다 (p<0.05). 한편, 이 두 병원체를 접종하지 않은 진정 대조군의 1일 령의 모체이행항체가 $log_2{\;}5.16{\;}{\pm}{\;}1.39$는 시간이 경과함에 따라 점점 낮아져 6주령에 이르러 완 전히 음전하였다. 이는 직선적인 변화를 하였으며, 주를 함수 (X)로 한 Y = -0.90X + 4.67의 일차방정식에 잘 부합하였으며, 실측치와 추정치와의 상관계수는 R = 0.9454이었다. 닭와포자충 감염 병아리는 건강한 것에 비하여 닭전염성기관지염 바이러스에 대한 면역억제현상이 일어나 아마도 감수성이 증가할 것으로 생각된다.

• Pediatric Infection and Vaccine
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• 제18권2호
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• pp.117-123
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• 2011

목 적: 홍역, 유행성이하선염 및 풍진 예방접종 시행후 연령 증가에 따른 항체양성율의 변화를 조사한다. 방 법: 병원에 내원한 7-20세의 환아를 대상으로 잔여 혈청을 이용하여 IgG 항체를 검사하였다. 결 과: 총 408례 중 홍역은 275례로 67.4%, 유행성이하선염은 112례로 27.5%, 풍진은 367례로 90.0%의 양성율을 보였다. 양성자의 IgG 수치는 각각 $2.77{\pm}1.18$ Index, $2.08{\pm}1.29$ Index 및 $60.46{\pm}63.47$ IU/mL 이었다. 결 론: 홍역 67.4%, 유행성이하선염 27.5% 및 풍진 90.0%의 IgG 항체 양성율을 보였다. 홍역, 유행성이하선염 및 풍진의 유행을 막기 위해서는 높은 접종율의 유지가 중요하며, 또한 보호자에 대한 접종기록 등의 관리가 중요할 것으로 생각된다.

• Journal of Korean Biological Nursing Science
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• 제15권1호
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• pp.24-32
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• 2013

Purpose: The purpose of this study was to investigate and compare human papillomavirus (HPV) vaccination status associated with HPV health belief and knowledge between male and female highschool students. Methods: In the winter of 2012, 183 students responded to self-administered questionnaires, and t-test, ${\chi}^2$-test, and ANOVA were used for data analysis. Results: There were significant differences in the experiences of hearing about HPV vaccination, source of HPV information, experience of being vaccinated with HPV vaccination, reason for HPV unvaccination and vaccination intention by gender. The mean scores for health belief of HPV vaccination were 1.72 (total score: 4) in men and 2.31 in women. There were significant differences in HPV health belief (t=-5.14, p<.001). The mean scores for knowledge of HPV vaccination were 0.08 (total score: 1) in men and 0.12 in women, but there were no significant differences in HPV knowledge (t=-1.62, p=.108). There were positive associations between HPV health belief and knowledge in women (p=.02). Conclusion: This study showed different HPV vaccination status, health belief and knowledge in gender. The results indicate a need to consider levels of health belief and knowledge of HPV vaccination in target populations of highschool students when planning a HPV related education program.

• 여성건강간호학회지
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• 제26권1호
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• pp.37-48
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• 2020

Purpose: The purpose of this study was to identify the factors influencing mothers' intention to vaccinate their elementary-school sons against human papillomavirus (HPV). Methods: The participants were 151 mothers of fourth- to sixth-grade boys at three elementary schools in Daejeon, Korea. The data were analyzed using SPSS for Windows version 25.0. Descriptive statistics, the independent t-test, one-way analysis of variance, the Mann-Whitney U test, Pearson correlation coefficients, and multiple regression analysis were used. Results: The mothers' score for intention to vaccinate their sons against HPV was 5.04 out of 7. Self-efficacy (β=.60, p<.001) and subjective norms towards HPV vaccination (β=.30, p<.001) were significant factors influencing mothers' intention to vaccinate their elementary-school sons against HPV. These factors accounted for 81.0% of HPV vaccination intention among mothers (F=160.84, p<.001). Conclusion: The results of this study suggest that to increase the HPV vaccination rate for elementary-school boys, it is necessary to develop intervention strategies to improve mothers' self-efficacy and subjective norms towards HPV vaccination and to verify the effects of those strategies. This research provides a foundation for designing interventions to increase the HPV vaccination rate of elementary-school boys.

• 지역사회간호학회지
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• 제21권4호
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• pp.502-511
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• 2010

Purpose: The purpose of this study was to investigate the influencing factors of influenza vaccination in the elderly participating in lifetime transitional health examination. Methods: This study was a secondary analysis of data collected from lifetime transitional health examination (for 66-year-old people) conducted by the National Health Insurance Corporation (NHIC) from January 1 to December 31, 2008. Questionnaires were received from NHIC to obtain information regarding gender, chronic diseases, health-related behaviors, and ADL. A total of 255,333 participants who responded all the questions in the questionnaire were included in the analysis. Collected data were analyzed by descriptive statistics, ${\chi}^2$ test, and multiple logistic regression. Results: The influenza vaccine coverage rate in 66-year-old people was 66.1%. The influenza vaccine coverage rate was higher in female elders and those with hypertension, diabetes, heart disease or past smoking, and lower in those with stroke, current smoking, drinking, no-exercise or ADL-dependency. Conclusion: Strategies for improving the influenza vaccination coverage rate in the elderly are needed. The strategies should give priority to the elderly with current smoking, drinking, and no-exercise, and home visiting programs are needed for the elderly with stroke and ADL-dependency.

• 여성건강간호학회지
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• 제17권3호
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• pp.205-214
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• 2011

Purpose: The purpose of this study was to examine womens' perceptions regarding influenza vaccination during pregnancy among Korean childbearing women. Methods: Data was collected using focus group interviews from June to September, 2010. Forty Korean women participated in 13 focus groups. After obtaining permission from participants, each session of the focus group was audio-taped and transcribed verbatim. The responses were analyzed utilizing qualitative content analysis. Results: Forty women ranging from 26 to 43 participated in the study. The major themes were safety concerns; effects of fetal protection and infection prevention; lack of perceived needs; and encouragement as well as concerns from others. Participants raised questions on whether the vaccine was safe and effective, and concerns about the potential harmful effect of influenza vaccine to their bodies and the fetus. The major reason for influenza vaccination during pregnancy was to protect self and fetus. Also, clinician's recommendation was the facilitating factors for influenza vaccination during pregnancy. Conclusion: The findings suggest that concerns associated with influenza vaccination should be considered when educating childbearing women about the influenza vaccine during pregnancy.

• 여성건강간호학회지
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• 제17권4호
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• pp.415-425
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• 2011

Purpose: This study was done to identify and compare factors associated with intention to receive Human Papillomavirus (HPV) vaccine between male and female undergraduate students. Methods: In the fall of 2010, 479 students responded to self-administered questionnaires about their intention to receive HPV vaccine, HPV knowledges, HPV related involvements and optimistic bias, subject's characteristics including opinion about HPV vaccine. Mann-Whitney U test, uni-variate and multi-variate logistic regression were used for data analysis with SPSS/WIN. Results: Intention to receive vaccine were 22.0% of men and 25.0% of women. There were significantly different in HPV knowledge (Z=-2.74, p=.006), optimistic bias (Z=-4.60, p<.001), vaccine is necessary for women (Z=-4,30, p<.001), vaccine is necessary for men (Z=-4.37, p<.001), vaccine is necessary if only symptom exist (Z=-4.52, p<.001), but there were not different in intention to receive vaccine, involvement between men and women. Concern about vaccine safety (OR=3.19, 95% CI 1.63~6.23) was determinant of intention to receive HPV vaccine for men. Conclusion: This study showed gender differences in HPV knowledge, HPV related optimistic bias and opinion about HPV vaccine, which would be assessed and well managed in tailored HPV education for enhancing HPV vaccine acceptance.

• 종양간호연구
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• 제11권3호
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• pp.171-178
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• 2011

Purpose: This literature review was conducted to provide a summary of the findings from research on knowledge and attitudes about human papilloma virus (HPV) and HPV vaccination, and studies of its actual uptake among women in Asian countries. Methods: The author searched the Pubmed, CINAHL, and KISS electronic databases to identify peer-reviewed articles published between 2006 and 2011. Results: Seventeen peer-reviewed studies met the inclusion criteria (13 quantitative, 4 qualitative). Findings from seven Asian countries that measured female adult's knowledge of HPV related issues and attitudes toward HPV vaccination were reviewed. Low level of knowledge about HPV and its related conditions, willingness to be vaccinated, and low uptake of vaccines were identified across the studies. Cultural barriers and social stigmatization about HPV vaccination were also discussed. Conclusion: Findings from this review indicate that adult women in Asian countries are in urgent need of improving HPV related knowledge and its actual vaccination. Policy makers, health care providers, and public health educators should take into account the cultural barriers and attitudes toward HPV vaccination in the process of developing and implementing educational programs and interventions for adult women in Asian countries.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1993년도 제2회 신약개발 연구발표회 초록집
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• pp.144-144
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• 1993

한국에서 유행하는 페염균 type을 결정하기 위한 기초 실험을 수행하여 서울대병원과 서울중앙병원의 환자로부터 분리한 88개의 폐염균주를 담즙산에 의한 용해도 및 Optochin에 대한 민감성 등을 측정하고 폐염균의 type을 결정하기 위해서 Double diffusion, Countercurrent immunoelectrophoresis, Rocket immunoelectrophoresis 방법 등으로 폐염균 항혈청과 반응하였다. 또한 ATCC로부터 구입한 폐염균주를 이용하여 배지조성의 변화에 따른 폐염균 생장을 측정하기 위해 CAT broth, Brain Heart Infusion broth, Defined Media에서 3가지 type의 균주(Type I, III, IV)를 접종하고 37$^{\circ}C$에서 배양하면서 1시간마다 550nm에서 흡광도를 측정하였다. 3가지 type (Type I, III, IV)의 폐염균주로부터 협막다당류를 phenol Iysis 추출법으로 폐염균 Capsule 다당류의 분리하여 Total Sugar 함량, Uronic acid 함량, 0-Acetyl 함량, 단백질 함량, 핵산 함량, 분자량분포 Countr-current immunoelectrophoresis 등을 실시하여 폐염균 백신 규격에 부합되는지 확인하였다. 수집한 88개의 폐염균주가 모두 담즙산에 의해 용해되고 6mm Optochin disc를 사용하였을때 성장 저지원이 모두 14mm 이상으로 나타나 감수성이 있어서 폐염균임을 확인하였으며 폐염균의 type 결정에 필요한 방법을 설정하였다. 배지조성에 따른 폐염균의 생장을 측정한 결과 CAT broth 에서는 Type I만이 잘 배양되었고 Type III, IV는 늦게 배양되었으나, Brain Heart Infusion broth에서는 3가지 Type이 모두 잘 배양되었고, Defined Media 에서는 Type I만이 성장되었을 뿐 나머지 두 Type은 배양되지 않았다. 본 실험방법으로 분리된 폐염균 다당류는 폐염균 백신 규격중의 6가지 항목에서 적합하였으며 Statens(Denmark)로부터 구입한 항체와 뚜렷한 침강선을 나타내었다.