• Title/Summary/Keyword: 배지영양

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Selective Media for Isolation and Enumeration of Lactic Acid Bacteria from Kimchi (김치에서 젖산균의 선발 및 계수를 위한 선택배지)

  • 이명기;박완수;강국희
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.5
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    • pp.754-760
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    • 1996
  • Selective media for isolation and enumeration of lactic acid bacteria from kimchi were compared using 36 strains of lactic acid bacteria from kimchi and dairy products. Among the selective media, KF Streptococcus agar showed the best result for the selection of Enterococcus group and Pediococus group, and M-17 agar with pH indicator for Lactococcus group, the m-LBS agar for Lactobacillus group, and PES-3 agar for Leuconostoc group.

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Physiological Activities of Mycelial Flammulina velutipes Cultured in Liquid Grain Media (곡물 액체배지에서 배양시킨 팽이버섯 균사체의 생리활성)

  • 한서영;손미예;이상원
    • Food Industry And Nutrition
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    • v.8 no.1
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    • pp.50-56
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    • 2003
  • 팽이버섯 균사체를 액체배양하여 전통 장류 발효식품및 음료개발에 이용할 목적으로 7종류의 곡물에 배양한 팽이버섯 배양액의 생리활성을 검토하였다 혈전 용해능은 골목 배양액과 침전물사이에는 차이가 없었으며, 대체로 조, 대두박 및 검정콩 배지에서 혈전용해능이 높게 나타났다. 항균력은 S.aureus에 대해서는 밀, 보리 및 검정콩이 높았고, L. plantarum에 대해서는 조, 밀 및 보리가 높았으며, E.coli에 대해서는 밀과 보리 배지에서 배양한 배양액이 높게 나타났다. Linoleic acid에 대한 항산화 효과는 곡물배지 자체에서는 검정콩, 대두박 및 합성배지에서 높게 나타났고, 팽이버섯을 배양했을 때는 검정콩배지의 항산화력과 유사한 활성을 나타내었다. 비장세포의 증식능은 보리, 밀, 조 및 합성배지에 팽이버섯을 배양하였을 때가 곡물자체에서보다 약 20% 정도 높게 나타났다. ConA를 버섯배양 추출물에 혼합했을 때는 옥수수, 대두박 및 검정콩에서 팽이버섯을 배양한 배양액이 $22\sim26%$ 정도의 증식효과가 있었으며, LPS를 혼합했을 경우는 옥수수, 대두박, 검정콩배지에 배양한 배양액이 각각 45%, 25%,18%의 증식효과가 나타내었다.

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Comparison of Physico-chemical and Nutritional Characteristics of Pre-inoculation and Post-harvest Flammulina velutipes Media (팽이버섯 접종전과 수확후 배지의 이화학성 및 영양성분 비교)

  • Cheong, Jong-Chun;Lee, Chan-Jung;Suh, Jang-Sun;Moon, Yea-Hwang
    • Journal of Mushroom
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    • v.10 no.4
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    • pp.174-178
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    • 2012
  • This study were carried out to analyze nutritional ingredients of pre-inoculation and post-harvest Flammulina velutipes media. pH, moisture content, total carbon(T-C), total nitrogen(T-N), crude protein, crude fiber, and crude fat content on the 8 raw materials using Flammulina velutipes bottle cultivation medium, and after sterilization(pre-inoculation) and post-harvest medium was analyzed. This result is expected to be utilized as the basis for recycling post-harvest media.

Studies on the effect of Grand emulsifiable concentrate in the various nutritional condition of Fusarium oxysporium f. cucumerinum (오이만할병균에 대한 영양조건에 따르는 Grand유제의 약효학적 연구)

  • Baek Soo-bong;Kim Myung-woon
    • Korean journal of applied entomology
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    • v.8
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    • pp.19-23
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    • 1969
  • It was observed that the effect of Grand E.C, on the growth of Fusarium oxysporium f. cucumerinum mycellia, which was cultured in the media with different nitrogen and carton sources, varied with it's concentrations. (1) Mycellia of F. oxysporium f. cucumerinum were grown strongly in $(NH_4)_2HPO_4\;NH_4NO_3$ and fructose media. (2) In general, the diluted Grand E.C, $ (\times\;1,000)$ effected on check of fungal growth in any type of media with the different nitrogen and carbon sources. It was disturbed the growth of fungi in $NH_4NO_3$, lactose and fructose in the diluted fungicide $(\times\;5,000)$. In the diluted solution $(\times\;10,000)$ of fungicide, somewhat increased the viability of fungi, but it was disturbed the growth in $NH_4NO_3$ and fructose media. (3) In conclusion, it was thought to be that the diluted Grand emulsion effected in check of the growth of F. oxysporium f. cucumerinum which grown some nutritional conditions.

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Studies on the Short Fermentation of the Mushroom Compost with Rice Straw (양송이 퇴비배지(堆肥培地)의 단기발효(短期醱酵)에 관한 연구(硏究))

  • Shin, Gwan Chull;Park, Jeong Sik
    • Korean Journal of Agricultural Science
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    • v.10 no.1
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    • pp.90-96
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    • 1983
  • The short period fermentation of the mushroom compost was studied as a method for the labor saving and mechanization of composting. Rice straw was crushed, watered and supplemented with organic nutrient sources. The straw-nutrient mixtures prepared were stacked and turned for short periods at the phase I and the phase II was carried out for 8 days. The total nitrogen of the short compost was lower than that of the conventional straw compost and it caused the yield decrease. The productivity of the short compost could be improved by the supplement of nurient sources. The effective nutrients were perilla meal and rice bran. Higher microflora of the rice straw-nutrient mixture during fermentation could shorten the composting period. For the short compost method the proper period of the phase I was 5 days.

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The Observation and a Quantitative Evaluation of Viable but Non-Culturable Bacteria in Potable Groundwater Using Epifluorescence Microscopy (형광현미경을 이용한 음용 지하수내 배양불능 세균의 관찰 및 정량적 평가)

  • ;Takashi Someya
    • Korean Journal of Microbiology
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    • v.38 no.3
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    • pp.180-185
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    • 2002
  • The direct viable count (DVC) and plate count (PC) methods was used to measure the number of bacteria in potable groundwater samples collected from bottled water from the market, mineral water, and edible groundwater near the urban areas and the stock farming congested areas. As a result, the number of living bacteria by DVC was comprised 30~80% of the total direct count (TDC), whereas the number of living bacteria by PC was around l~30% of DVC. Such results show that viable but non-culturable (VBNC) bacteria exist in the potable groundwater with high percentages. On the other hand, upon measuring the value from the conventional nutrient broth (NB), $10^-2$ fold diluted nutrient broth (DNB), and R2A broth, the values from the DNB and R2A showed 2~50 times higher than the conventional NB medium. These results indicate that oligotrophic bacterial groups which can multiply in the low nutrient broth abundantly exist in the oligotrophic environment like potable groundwater.

Utilization of various substrates for the cultivation of oak mushrooms (Lentinula edodes) (다양한 기질에 대한 표고버섯 톱밥재배 가능성)

  • Jeong, Yeun Sug;Jang, Yeongseon;Ryoo, Rhim;Ka, Kang-Hyeon
    • The Korean Journal of Mycology
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    • v.47 no.4
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    • pp.373-383
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    • 2019
  • We investigated the effect of different media components on the characteristics and productivity of Lentinula edodes cultivated in sawdust. Ten substrates were used for this study. Soybean and cotton seed meal were unsuitable substrates as fungal mycelia did not grow to maturity during the incubation period. Lentinula edodes (NIFoS culture number 2462) was grown on the ten substrates, and soybean hull, mixed medium, cotton seed hull and corn grain showed greater effect on the productivity than wheat bran. However, wheat bran induced higher productivity in NIFoS 2778 than the other substrates. Sawdust medium with soybean meal produced smaller fruiting bodies than the other substrates. In contrast, corn meal media produced larger mushrooms than the other substrates. The external characteristics of the mushrooms varied based on the substrates upon which they were grown. This is not surprising given that the substrates differed with respect to carbohydrate and protein content, e.g., cotton seed and soybean meal contained a higher crude protein and crude ash than the other substrates tested.

Growth of Strawberry Plantlets Cultured in Vitro in the Agar or Commercial Plug Medium as Affected by Ionic Strength (이온강도에 따른 Agar와 공정육묘용 상토에서 기내배양된 딸기 소식물체의 생육)

  • Hwang, Seung-Jae;Jeong, Byoung-Ryong
    • Horticultural Science & Technology
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    • v.30 no.2
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    • pp.201-207
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    • 2012
  • This study was conducted to investigate the effect of supporting material and ionic strength of the MS medium on growth of strawberry plantlets cultured in vitro for the rapid mass production. Explants of $Fragaria$ $ananassa$ 'Houkouwase' in vitro were planted in the agar or Tosilee (commercial plug medium) medium as the supporting material and supplied with 1/4 MS, 1/2 MS or basal (as the control) MS medium in an autotrophic micropropagation. Plant height and root length were significantly greater when they were cultured in 1/2 MS medium as compared to those grown in the agar medium. Also, shoot fresh and dry weights, and leaf area in the 1/2 MS medium were greater than in 1/4 MS or basal MS medium. When plantlets were cultured in Tosilee medium and fed with the basal MS medium, plant height, root length, shoot fresh and dry weights, root fresh and dry weights, and leaf area were promoted and greater than those in plantlets cultured in the agar medium.

토지이용에 따른 영양염류 항목의 유출특성 연구

  • Yun, Yeong-Sam;Sin, Seok-Ho;Yu, Jae-Jeong;Sin, Chan-Gi
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 2008.11a
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    • pp.388-391
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    • 2008
  • 과수원재배지 및 비닐하우스재배지의 투수지역과 공업지역 및 교통지역의 불투수지역에 대한 영양염류 중심의 강우 유출수 조사결과 TN 항목의 경우 불투수지역인 공업과 교통지역에서 각각 9.88 mg/L, 16.50 mg/L로 높은 값을 보였으며, TP의 경우 비닐하우스재배지와 교통지역에서 각각 6.64 mg/L와 2.28 mg/L로 높게 조사되었다.

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Photomixotrophic Growth of Solanum tuberosum L. in vitro with Addition and Omission of Organic Materials at Thee Initial Sucrose Levels in the Medium (세 수준의 자당이 첨가된 배지에서 유기물의 첨가 유무에 따른 Solanum tuberosum L.의 기내 광혼합영양생장)

  • Jeong, Byoung-Ryong;Yang, Chan-Suk;Kim, Gyeong-Hee;Park, Young-Hoon;Kozai, Toyoki
    • Journal of Bio-Environment Control
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    • v.13 no.1
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    • pp.51-55
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    • 2004
  • The most commonly used inorganic nutrient compositions such as Murashige & Skoog medium have been optimized for heterotrophic growth. Therefore, they may not be optimal for photomixotrophic and photoautotrophic growth of plantlets. In photomixotrophic micropropagation, emdium sugar level is often lowered, while light and $CO_2$ levels in vessel are raised, and chlorophyllous explants are used to facilitate photosynthetic carbon acquisition. In a factorial experiment effect of addition (+) and omission(_) of organic materials (OM, 0.5 g ${\cdot}$ $m^{-3}$ each of thiamine, nicotinic acid and pyridoxine and 100 ${\cdot}$ $m^{-3}$ myo-inositiol) combined with three sucrose levels (0, 15, and 30 kg ${\cdot}$ $m^{-3}$) was tested on the growth of potato plantlets. Each of nodal cuttings with a leaf was cultured on 0.1${\times}$$10^{-4}m^{-3}$) MS agar ( 8 kg ${\cdot}$ $m^{-3}$) medium (pH 5.80 before autoclave) in glass test tubes (100 mm${\times}$25mm) capped with a sheet of transparent film with a 6 mm diameter gas permeable filter (5.1 air exchanges ${\cdot}$$h^{-1}$). Cultures were maintained in a room for 27 days at $23^{\circ}C$, 50% RH, 350-450${\mu}mol\;{\codt}\;mol^{-1}CO_2$, 16 h ${\cdot}$ $d^{-1}$ photoperiod at 13${\mu}mol\;{\codt}\;m\;{\codt}\;s^{-1}$ PPFD provided by white cool fluorescent lamps. Growth of potato plantlet in the +OM and -OM treatments were similar, while medium pH was 0.2 scale lower in the latter. Dry weight, % dry matter, and stem diameter enhanced, while shoot to root dry weight ratio, leaf area, chlorophyll concentration per gram dry weight, and medium pH decreased with increasing initial sucrose level. Interaction between OM and sucrose levels was observed in shoot length and medium pH. Results indicate that OM can be omitted from the medium without detrimental effect while addition of sucrose was beneficial for the photomixotrophic growth of potato plantlets under raised light and $CO_2$.