DOI QR코드

DOI QR Code

Utilization of various substrates for the cultivation of oak mushrooms (Lentinula edodes)

다양한 기질에 대한 표고버섯 톱밥재배 가능성

  • Jeong, Yeun Sug (Division of Special Forest Products, National Institute of Forest Science) ;
  • Jang, Yeongseon (Division of Special Forest Products, National Institute of Forest Science) ;
  • Ryoo, Rhim (Division of Special Forest Products, National Institute of Forest Science) ;
  • Ka, Kang-Hyeon (Division of Special Forest Products, National Institute of Forest Science)
  • 정연석 (국립산림과학원 산림소득자원연구과) ;
  • 장영선 (국립산림과학원 산림소득자원연구과) ;
  • 유림 (국립산림과학원 산림소득자원연구과) ;
  • 가강현 (국립산림과학원 산림소득자원연구과)
  • Received : 2019.12.09
  • Accepted : 2019.12.16
  • Published : 2019.12.31

Abstract

We investigated the effect of different media components on the characteristics and productivity of Lentinula edodes cultivated in sawdust. Ten substrates were used for this study. Soybean and cotton seed meal were unsuitable substrates as fungal mycelia did not grow to maturity during the incubation period. Lentinula edodes (NIFoS culture number 2462) was grown on the ten substrates, and soybean hull, mixed medium, cotton seed hull and corn grain showed greater effect on the productivity than wheat bran. However, wheat bran induced higher productivity in NIFoS 2778 than the other substrates. Sawdust medium with soybean meal produced smaller fruiting bodies than the other substrates. In contrast, corn meal media produced larger mushrooms than the other substrates. The external characteristics of the mushrooms varied based on the substrates upon which they were grown. This is not surprising given that the substrates differed with respect to carbohydrate and protein content, e.g., cotton seed and soybean meal contained a higher crude protein and crude ash than the other substrates tested.

본 연구에서는 표고 톱밥재배의 배지 조성에 따른 생산성 및 버섯 특성에 대해 알아보기위해 총 10가지의 농업부산물을 영양원으로 사용하였다. 대두박과 면실박을 이용한 배지에서는 배양기간 동안 균사가 완전히 생장하지 않아서 재배에 이용할 수 없었다. NIFoS 2462 균주에서는 대두피, 면실피, 파옥쇄, 혼합배지에서는 밀기울보다 높은 생산량을 나타내었으며, NIFoS 2778에서는 밀기울의 생산량이 다른 영양원들보다 높게 나타났다. 대두피를 사용한 배지에서 발생한 버섯들은 다른 배지에서 발생된 버섯들보다 작은 크기의 버섯이 생산되었으며, 반대로 옥분을 이용한 배지에서는 다른 영양원을 사용한 배지보다 큰 버섯이 생산되었다. 이를 통해 배지에 첨가되는 영양원이 버섯의 특성에 영향을 주는 것으로 확인되었다. 영양원들의 기초 성분 조사 결과, 대두박과 면실박은 조단백질과 조회분의 비중이 높았다. 그리고 면실박에서는 총유기물 함량과 가용성무질소물의 양이 다른 영양원들에 비해 낮게 나타났다. 이러한 영양원들의 기초성분이 버섯 재배시 생산량 및 버섯의 특성에 영향을 준다고 여겨지며, 이와 같은 표고 재배에 대한 연구를 통하여 다양한 자원에 대한 활용 가능성을 넓히는 것이 중요하다.

Keywords

References

  1. Chang ST, Buswell JA. Mushroom nutriceuticals. World J Microbiol Biotechnol 1996;12:473-6. https://doi.org/10.1007/BF00419460
  2. Sharma VP, Kumar S, Kumar R, Singh R, Verma D. Cultural requirements, enzyme activity profile, molecular identity and yield potential of some potential strains of shiitake(Lentinula edodes). Mushroom Res 2013;22:105-10.
  3. Sugui MM, Lima PLA, Delmanto RD, Eira AF, Salvadori DMF, Ribeiro LR. Antimutagenic effect of Lentinula edodes (Berk.) Pegler mushroom and possible variation among lineages. Food Chem Toxicol 2003;41:555-60. https://doi.org/10.1016/S0278-6915(02)00306-X
  4. Khan MA, Khan LA, Hossain MS, Tania M, Uddin MN. Investigation on the nutritional composition of the common edible and medicinal mushrooms cultivated in Bangladesh. Bangladesh J Mushroom 2009;3:21-8.
  5. Ozcelic E, Peksen A. Hazelnut husk as a substrate for the cultivation of shiitake mushroom(Lentinula edodes). Bioresour Technol 2007;98:2652-8. https://doi.org/10.1016/j.biortech.2006.09.020
  6. Royse DJ. Effect of spawn run time and substrate nutrition on yield and size of the shiitake mushroom. Mycologia 1985;77:756-62. https://doi.org/10.2307/3793284
  7. Royse DJ, Sanchez JE. Ground wheat straw as a substitute for portions of oak wood ships used in shiitake(Lentinula edodes) substrate formulae. Bioresour Technol 2007;98:2137-41. https://doi.org/10.1016/j.biortech.2006.08.023
  8. Levanon D, Rothschild N, Danai O, Masaphy S. Bulk treatment of substrate for the cultivation of shiitake mushrooms (Lentinula edodes) on straw. Bioresour Technol 1993;45:63-4. https://doi.org/10.1016/0960-8524(93)90145-2
  9. Philippoussis A, Diamantopoulou P, Israilides C. Productivity of agricultural residues used for the cultivation of the medicinal fungus Lentinula edodes. Int Biodeterior Biodegradation 2007;59:216-9. https://doi.org/10.1016/j.ibiod.2006.10.007
  10. Kim YI, Bae JS, Huh JW, Kwak WS. Monitoring of feed-nutritional components, toxic heavy metals and pesticide residues in mushroom substrates according to bottle type and vinyl bag type cultivation. J Anim Sci Technol 2007;49:67-78. https://doi.org/10.5187/JAST.2007.49.1.067
  11. Kim SY, Jeong MH, Kim MK, Im CH, Kim KH, Kim TS, Kim DS, Cheong JC, Hong KS, Ryu JS. Composition analysis of raw material constituting the medium for mushroom cultivation. J Mushrooms 2013;11:208-13. https://doi.org/10.14480/JM.2013.11.4.208
  12. Philippoussis A. Diamantopoulou P, Zervakis G. Correlation of the properties of several lignocellulosic substrates to the crop performance of the shiitake mushroom Lentinula edodes. World J Microbiol Biotechnol 2003;19:551-7. https://doi.org/10.1023/A:1025100731410
  13. Silva EM, Machuca A, Milagres AMF. Effect of cereal brans on Lentinula edodes growth and enzyme activities during cultivation on forestry waste. Lett Appl Microbiol 2005;40:283-8. https://doi.org/10.1111/j.1472-765X.2005.01669.x
  14. Baldrian P. Fungal laccases-occurrence and properties. FEMS Microbiol Rev 2006;30:215-42. https://doi.org/10.1111/j.1574-4976.2005.00010.x