• Journal of the Korean Society of Radiology
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• v.13 no.5
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• pp.811-817
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• 2019

Radiation-therapy causes the adverse radiation effect. It is called osteoradionecrosis. A protein-therapy is carried out in order to cure osteoradionecrosis. The typical method of the protein-therapy is using BMP-2. Considering to bone damage, it is more important that maintains enough to circumstance regeneration for osteoblast differentiation on damage site of bone. Thus, this study is on a tissue regeneration to cure radiation critical damage. I observed that the formation of new regeneration bone by injection of collagen sheet BMP-2 on irradiated mouse. Consequently, I examined new bone formation with collagen sheet BMP-2 on irradiated mouse after 8weeks. Therefore I suggested that using collagen sheet BMP-2 which can be good for new bone regeneration effect on radiation side effect area.

• Journal of the Korean Society of Radiology
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• v.10 no.6
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• pp.375-379
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• 2016

A rat is the most common experimental animal used for the realization of the radiation injury model. The certain thickness of rat skin was prepared by peeling off a rat skin. Radiation level was measured by using this rat skin. Also, The schematic of the formula was made that can predict the radiation absorbed dose (RAD) as a function of the thickness of the rat skin. Consequently, we will provide the RAD information in the realization of in-vitro experimental model regarding the rat's skin thickness by applying the formulas. Moreover, the results from this study can be effectively used for the in-vitro experiment of the rat subcutaneous tissue which was exposed to radiation.

• Journal of the Korean Society of Radiology
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• v.15 no.4
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• pp.539-545
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• 2021

Radiation therapy is accompanied by adverse radiation effective. In particular, it is accompanied by disorders of the vascular system. Therefore, oxygen and nutrient deficiency occurs in the regeneration area. Eventually, osteoradionecrosis is formed in this cellular environment. According to a precedent study, bone morphogenetic protein-2 is used to overcome osteoradionecrosis. The purpose of this study was to investigate the regeneration ability of osteoradionecrosis by treating bone-forming protein-2 on a fibrinogen scaffold which is a biomaterial that is frequently used for bone regeneration after irradiation of the rat head. In addition, the purpose of this study was to verify the bone regeneration effect from the eight weeks. According to the experimental results, in the calvarial defected model of the irradiated mouse, making bone-formation was obtained after 8 weeks rather than bone-formation period in the early 4 weeks. moreover, it was found that the regenerated bone formation of the fibrinogen scaffold is formed from the inside of the bone of the defect area.

• Journal of Radiation Protection and Research
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• v.35 no.2
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• pp.49-56
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• 2010

Developments of radioprotective agents are important issues for minimizing the troubles and the effective treatments in radiotherapy. But few agents are useful in clinical and practical fields. It was shown that trace elements in alcohol beverages might have radioprotective effect. In this study, the types of cell death of lymphocytes according to the commercial alcohol beverage was investigated. Normal healthy volunteers ingested distilled water, beer or soju containing $81.5mg{\cdot}dl^{-1}$ ethyl ahcohol, respectively. After 2 hours, their blood were sampled with their consents. Fraction of lymphocytes was isolated by density gradient method with Histopaque-1077 (Sigma) and irradiated with dose from 0.5 to 5 Gy. After 60 hour incubation, the cells were harvested and analysed by flow cytometry. Cell viability was decreased by dose dependent manner. Cell viability of beer group was reduced about 15% compared with control group. Apoptosis in soju group was reduced about 20% compared with control group. Apoptosis of beer and control groups are similar. Necrosis of soju group significantly increased about 35% compared with control group. Early apoptosis of beer group was increased compared with control group. Early apoptosis of soju group was decreased about 25% compared with control group. Late apoptosis of beer and control group was increased by dose dependent manner. Late apoptosis of soju group was increased about 20-30% compared with control group. Late apoptosis of soju was increased and the radioprotective effect of soju was minimal because late apoptosis induced the cell necrosis. In case of soju trace elements, total cell apoptosis was decreased about 20% and early cell apoptosis was remarkably low. In this case, mitotic cells death may be dominant mechanism. Therefore, trace elements in soju may not be effective radioprotective agents.