• Food preservation and processing industry
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• v.7 no.2
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• pp.33-36
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• 2008

차는 차나무 잎을 가공한 것으로 녹차 등의 비발효차, 우롱차 등의 반발효차, 홍차 등의 발효차로 분류된다. 우롱차, 홍차 등의 발효는 미생물이 관여하는 발효가 아니고 찻잎에 존재하는 효소에 의한 반응을 이용하므로 효소 발효차로 불리운다. 반발효차는 $10{\sim}65%$ 발효도 그리고 발효차는 85% 이상의 발효도 제품으로 통상 정의되나 발효정도를 판단할 수 있는 기준이 설정되어 있지 않아 발효도 100%에 해당되는 위치를 구명하여 발효차 발효정도 판별 기준으로 활용하고자 발효과정 중 일어나는 성분과 색조의 변화를 검토하였다. 적색도(a 값)와 황색도(b 값)는 위조 및 유념 과정에서부터 색을 띠기 시작하여 발효 진행과 함께 증가하여 발효 시작 일정시간 후 최고점에 도달하고 이후 감소를 보였다. 가시영역의 녹차와 홍차의 스펙트럼에서 가장 큰 차이를 보인 420 nm 흡광도 값은 발효 진행과 함께 증가되어 일정시간 후 최고점에 도달하고 이후 감소를 보였다. 찻잎 성분 중 카테킨류는 5종이 검출되었는데 발효 진행과 함께 모두 급격히 감소하였다. 그러나 카페인은 발효 진행과 관계없이 거의 일정 수준을 유지하여 카페인은 효소 발효에 의해 변환되지 않는 성분으로 확인되었다. 4종의 테아플라빈류는 발효 0%에 해당하는 녹차에는 검출되지 않았으나 발효 진행과 함께 생성됨이 확인되었다. 테아플라빈류는 4종이 검출되었는데 이중 생성량이 많은 것은 TF3,3'G와 TF3G 순이었으며 이 성분들은 발효의 진행과 함께 일정시간 후 최고점에 도달하고 이후 감소를 보였다. 발효차 제조과정 중 발효에 의해 생성되는 적색과 황색 그리고 420 nm의 흡광도 값 그리고 발효에 의해 생성되는 TF3,3'G와 TF3G 생성 최고점에 이르는 발효시간이 일치함이 발견되었다. 이 최고점은 발효도 100%에 해당하는 위치로 판단된다.

• Korean Journal of Plant Resources
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• v.24 no.2
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• pp.236-242
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• 2011

In this present study, we investigated the anti-oxidant activity, the inhibition ability of lipid peroxidation, and the protective effect of cow pulmonary epithelium (CPAE) cells under oxidative stress using green tea and 3 types of fermented teas of Jeju Island. To compare the physiological activity of non-fermented and 3 types of fermented teas, the fermented time was controlled with 0 hr. (non fermented tea, G), 12 hrs. (20% fermented tea, F20), 17 hrs. (50% fermented tea, F50) and 24 hrs. (80% fermented tea, F80), respectively. Scavenging ability on DPPH radicals of 80 ${\mu}g/mL$ concentration of F20 was similar to that of 50 ${\mu}M$ epigallocatechin gallate (EGCG) but it was stronger than those of G, F50 and F80. All extracts tested inhibited LDL oxidation but G and F20 inhibited LDL oxidation 25~30% more than F50 and F80 at 40 ${\mu}g/mL$ concentration which was similar to that of 50 ${\mu}M$ EGCG. We observed that the CPAE cells treated with the tea extracts had a significant increase in cell viability, especially the cells under oxidative stress with 1 mM $H_2O_2$ as compared with the control group (no treatment with tea extracts). These findings suggested that all tea extracts containing fermented tea had a protective effect on oxidative stressed CPAE cells through their free radical scavenging activity. It can be concluded that F20 extracted from 20% fermented tea has the most significant antioxidative effects that inhibit lipid peroxidation and protect the CPAE cells under oxidative stress.

• Journal of Nutrition and Health
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• v.43 no.4
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• pp.333-341
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• 2010

The aim of this study was to investigate the hypolgycemic activity of water extract of fermented yacon (Smallanthus sonchifolius) leaves tea (Yacon LWE) in high-fat diet (HFD)/streptozotocin (STZ)-induced diabetic mice. Male ICR mice were fed with a HFD (37% calories from fat) for 4 weeks prior to intraperitoneal injection with STZ (100 mg/kg body weight). Diabetic mice were supplemented with two doses of Yacon LWE (0.16% and 0.8%, wt/wt) for 6 weeks. The supplementation of high-dose Yacon LWE significantly lowered blood glucose levels and plasma ALT and AST activities compared with the control group. High-dose Yacon LWE also improved the insulin tolerance without any changes in plasma and pancreatic insulin concentrations in HFD/STZ-induced diabetic mice. Yacon LWE supplementation increased the insulin staining of pancreatic $\beta$-cells in a dose-dependent manner. Both 0.16% and 0.8% of Yacon LWE significantly elevated plasma leptin concentration, hepatic glucokinase activity and glucokinase/glucose-6-phosphatase ratio compared with the control group. However, glycosylated hemoglobin concentration was not different among the groups. These results suggest that high-dose Yacon LWE lowers the blood glucose level partly by enhancing insulin sensitivity and hepatic glucose metabolism in type 2 diabetic mice.

• The Korean Journal of Food And Nutrition
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• v.25 no.4
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• pp.807-819
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• 2012

To evaluate the functional characteristic and availability for drinking of the fermented Smilax china leaf tea by using different microbial species, various fermented leaf tea was prepared by non-fermentation (C), or the fermentation of Saccharomyces cerevisiae (S), Bacillus sp. (B), Bifidobacterium bifidus (L), Monascus pilosus (M) and Aspergilus oryzae (A), and sensory and antioxidant parameter of each brewed tea was observed. The color of the A tea was red, but the other teas were yellow in color. Furthermore, the aesthetic quality of the A and M tea was 3.95 and 3.30 point, respectively, and other teas (2.55~2.28) were similar to that of the C tea. TP of fermented tea water extract was lower than that of the C, although TF was not significantly different between the fermented and non-fermented tea. Especially, TF of the A tea was significantly lower than those of the other teas. The range of EDA ($1mg/m{\ell}$) of water and ethanol extracts of tea C and the fermented teas was 19.25~22.48%; however, tea A was only 8.04~12.49%. In addition, FRAP, FICA and LPOIA of teas were not significantly different between the fermented and non-fermented teas. On the other hand, XOIA and AOIA of tea ethanol extracts were slightly higher than those of water extracts. XOIA of water extract derived from the teas was 4.83~9.20%, while ethanol extract of these was 9.00~19.00%. However, XOIA of B and L teas water extract was not detected. Furthermore, AOIA of fermented tea water extract (30.17~48.52%) were lower than those of ethanol extract (44.09~66.93%). In this study, interestingly, antioxidant parameters, such as FRAP, FICA, LPOIA and AOIA, of the A tea water extract (0.1%) was higher than that of the other tea in spite of high decreasing rate in the contents of TP and TF. Therefore, above results imply the possibility of fermented Smilax china leaf tea as a functional food.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.5
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• pp.594-600
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• 2009

Quality characteristics of white lotus leaf tea (LLT) fermented with or without mycelial Paecilomyces japonica were investigated. Extraction yield and browning index of hot water extract from non fermented and fermented LLTs were higher than those of ethanol extract (p<0.05). In all LLTs, nutritional components such as total free sugar, free amino acids and minerals of hot water extracts were higher than those of ethanol extracts except for total organic acids (p<0.05). Contents of total free sugar and organic acids were markedly increased through fermentation process of mycelial Paecilomyces japonica. in the same solvent extracts (p<0.05). Contents of most taste components of fermented LLT were increased by mycelial solid fermentation (p<0.05), but total free amino acids of two extracts were decreased in the range of $37.1{\sim}67.2%$ as compared to non-fermented LLT. Fifty-nine volatile compounds were identified by GC and GC-MS, including 11 aldehydes, 14 alcohols, 11 ketones, 11 hydrocarbones and 12 acids. Aldehyde and ketone compounds were more identified in fermented LLT than in non-fermented LLT being abundant alcohol compounds by simultaneous steam distillation and extraction. The most abundant compounds of LLT identified in this study were curcumene followed by 2,6-bis(1,1-dimethylethyl)-4-methyl-phenol and cyclohexen. Main compounds of fermented LLT were 2,6-bis(1,1-dimethylethyl)-4-methyl-phenol, butanoic acid, furfural, benzaldehyde, hexanoic acid and 2(3H)-furanone.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.10
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• pp.1703-1708
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• 2004

This study was carried out to investigate the characteristic quality of jeungpyun added Paecilomyces japonica with different fermentation time. The volume of jeungpyun added Paecilomyces japonica was more increased after fermentation. The volume was decreased when the fermentation was progressed. The pH was 5.1 before fermentation and the pH decreased when the fermentation was progressed. Moisture content was more increased as the time of fermentation became longer. Hunter color test, L value and b value appeared the highest after 1st fermentation, and it appeared lesser when the time of fermentation became longer. In measurement of texture, hardness showed the highest value when 90 minutes passed after the 2nd fermentation. Gumminess and brittleness were tend to increase when it have longer fermentation time. In scanning electron microscopic observation, organization of sponges was tend to break down as the fermentation time was longer. Sensory properties showed that color intensity was more strong when second fermentation was progressed, and hardness, toughness, sourness were high when the fermentation time was longer. In conclusion overall acceptability of jeungpyun, 3$0^{\circ}C$, 3 hours after 1st fermentation and 2nd fermentation for 30 minutes, 35$^{\circ}C$ showed the most preference.

• Proceedings of the Korean Society of Organic Agriculture Conference
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• 2009.12a
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• pp.133-139
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• 2009

1) 공시유기질비료의 전질소는 유기발효돈분(2.68 %) > 계분부산물퇴비(1.97 %) > 유기발효계분, 돈분부산물퇴비(1.92 %) > 유기퇴적우분(1.12 %) > 유기 생 우분(1.04 %) 순이었다. 또한 유기물 함량은 유기 생 우분(81.73 %) > 유기퇴적우분(75.52 %) > 계분부산물(68.25 %) > 유기발효돈분(63.37 %) > 돈분부산물(45.27 %) > 유기발효계분(32.05 %) 순이었다. 2) 수수$\times$수단그라스 교잡종의 건물수량은 1차에서는 유기발효계분(11.60 ton/ha)이 가장 많았으나 계분부산물퇴비(10.13 ton/ha), 돈분부산물퇴비(9.07 ton/ha)와의 유의차는 없었고, 2차 역시 유기발효계분(8.00 ton/ha)이 가장 많았으나 계분부산물퇴비(6.27 ton/ha), 돈분부산물퇴비(6.13 ton/ha)와의 유의차는 없었다(p<0.05). 3) CP 수량은 1차에서는 유기발효계분(0.69 ton/ha)이 가장 많았으나 돈분부산물퇴비(0.56 ton/ha), 계분부산물퇴비(0.55 ton/ha), 유기 생 우분(0.55 ton/ha)과의 유의차는 없었고, 2차에서는 유의차가 없었다(p<0.05). 4) TDN 수량은 1차에서는 유기발효계분(6.51 ton/ha)이 가장 많았으나 계분부산물 퇴비(5.80 ton/ha), 돈분부산물퇴비(5.08 ton/ha)와의 유의차는 없었고, 2차 역시 유기발효계분(4.80 ton/ha)이 가장 많이 나왔으나, 계분부산물퇴비(3.70 ton/ha)와 유의차는 없었다(p<0.05). 5) 상대사료가치(RFV)는 1차에서는 계분부산물퇴비 시비구가 80.66으로 유의하게 높았으나, 2차에서는 유기퇴적우분을 제외한 5개 시비구의 유의차는 없었다(p<0.05). 6) K는 유기발효계분(0.11 head/ha/yr)이 가장 높았으나 계분부산물퇴비(0.10 head/ha/yr), 돈분부산물퇴비(0.09 head/ha/yr)와 유의차는 없었고, $K_{CP}$ 역시 유기발효계분(7.23 head/ha/yr)이 가장 높게 나왔으나 돈분부산물퇴비(6.41 head/ha/yr), 유기 생 우분(6.15 head/ha/yr), 계분부산물퇴비(5.97 head/ha/yr)와의 유의차는 없었다(p<0.05). 또한 KTDN은 유기발효계분(8.90 head/ha/yr)이 가장 높게 나왔으나 계분부산물퇴비(7.49 head/ha/yr)와 유의차는 없었다(p<0.05). 7) 이상의 결과로 보았을 때, 유기발효계분이 수단그라스의 생산성, 사료가치 및 가축사육능력에서 가장 우수할 것으로 판단된다.

• Proceedings of the SOHE Conference
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• 2003.10a
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• pp.84-84
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• 2003

본 연구는 차는 잎 자체가 가지고 있는 상쾌한 향과 차 제조과정 중에 생성되는 독특한 향으로 인하여 기호식품으로서 뿐만 아니라 체내의 생리활성 작용이 탁월하기 때문에 기능성식품으로서 수요가 증가되고 있다. 차의 풍미는 차 생산지의 토양, 기후, 품종, 제조방법 등 여러 요인에 의하여 영향을 받으나 제일 중요한 것은 제조방법으로 차의 산화효순에 의한 발효정도에 따라 차의 향과 맛, 체내작용 등에 차이가 있다. 국내산 반발효차 및 발효차는 수입된 우롱차와 홍차와는 차별화된 은은한 향과 깊은 맛을 지니며, 기호도가 매우 높다.

• Korean Journal of Clinical Laboratory Science
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• v.52 no.4
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• pp.398-407
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• 2020

The inhibitory effect on α-glucosidase, a marker of postprandial hyperglycemia, and angiotensinconverting enzyme (ACE), a marker of hypertension, was analyzed using non-fermented green tea and three different types of fermented tea, which are popular beverages in modern life. Green tea was mixed with trace amounts of vanadate (50 ㎍/mL), which has insulin-mimetic effects, to investigate the synergistic effect of vanadate on the inhibition of α-glucosidase. The concentration of epigallocatechin gallate (EGCG) and caffeine was also checked. The extracts of green tea and fermented teas showed clear inhibition on α-glucosidase, which caused a decrease in the postprandial glucose levels. The inhibitory effect was most prominent in the 20% fermented tea. Trace amounts of vanadate (50 ㎍/mL)-mixed green tea extract had twice the inhibitory effect on α-glucosidase than the pure tea extract. All teas showed inhibitory effects on ACE. Among those, the effect was most prominent in green tea, which had higher concentrations of EGCG. In contrast, the postprandial glucose-lowering effect and ACE inhibition of the fermented teas, which have a lower level of EGCG, was attributed to some other different functional substances.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.3
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• pp.421-427
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• 2010

This study was conducted to compare and analyze the active and general components between mulberry leaf tea (MLT) and fermented mulberry leaf tea (FMLT). Among the general ingredients, the crude lipids of FMLT contained higher than that of MLT, while the level of carbohydrates was higher in the MLT. Although free sugar of FMLT was a lower concentration than MLT, the value of maltose was higher in the MLT. In FMLT, maltose was not analyzed but small amount of lactose was analyzed. In addition, the level of total free amino acid of MLT was higher than that of FMLT. The level of glutamic acid was highest in MLT, while that of cystine was highest in FMLT. The value of $\gamma$-aminobutyric acid (GABA) was greater in the derivatives of amino acids, and the content was similar for both MLT and FMLT. FMLT also contained a lower level of unsaturated fatty acids than MLT. In addition, the content of total mineral was higher in FMLT, and the level of K was the highest and similar in both teas.