• Journal of Veterinary Clinics
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• v.16 no.2
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• pp.474-477
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• 1999

A four-year old bitch which showed occasional hemomorragic exudate from the vulva for two weeks after the previous estrus 7 months ago was ovariohysterectomied. About 200m1 of sanguineous fluid was flowed out from the uterus and some scattered blood clots were found in both uterine horns. Hematometra of the bitch which was presumed to occur during the proestrus two weeks ago was thought to progress into early pyometra at the time of diagnosis. To diagnose hematometra, vaginal examination using vaginal speculum or vaginoscope and hematological examination were performed. Radiography and ultrasonography were also proved to be of use to diagnose hematometra. Treatment for hematometra was considered to be the same as for the case of pyometra.

• Proceedings of the Korea Information Processing Society Conference
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• 2013.11a
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• pp.1439-1441
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• 2013

본 연구에서는 비디오 감시 시스템을 기반으로 한우 축사에서 승가 행위 검출을 위한 최적의 카메라 위치를 결정한다. 실외 환경에서는 소들간의 겹침이나 조명 변화 등 다양한 어려움이 발생하기 때문에, 이를 극복하기 위하여 승가 시 소의 몸체가 올라간다는 특성을 이용한다. 즉, 등높이가 1.2m에서 1.3m 사이 크기의 한우를 사육하는 축사에서 축사 측면에 1.55m 높이로 카메라를 설치하고 여기서 획득된 영상으로 실험한 결과, 발정기 탐지를 위한 승가 행위를 자동으로 검출할 수 있음을 확인하였다.

• Korean Journal of Veterinary Research
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• v.37 no.2
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• pp.331-338
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• 1997

최근 동물의 진통 및 진정을 목적으로 널리 사용되고 있는 imidazole 유도체인 clonidine, medetomidine, etomidate 등의 약물과 xylazine의 효과를 발정정지기의 척출 돼지 자궁근에서 검토하였다. Clonidine($10^{-8}{\sim}10^{-6}M$)이나 medetomidine($10^{-8}{\sim}10^{-6}M$)은 xylazine과 비슷한 정도로 용량의존적인 자궁근의 수축을 일으켰다. Clonidine, medetomidine, xylazine 등의 $EC_{50}$는 각각 24.7nM, 19.9nM, 45.1nM이었다. 그러나 etomidate는 $10^{-6}M$ 미만의 농도에서 반응이 거의 없었으며, $10^{-6}M$ 이상에서 수축반응을 일으켰다. 이들 agonists의 효과는 yohimbine($10^{-8}{\sim}10^{-6}M$), idazoxan($10^{-7}{\sim}10^{-5}M$), tolazoline($10^{-7}{\sim}10^{-5}M$) 등의 ${\alpha}_2-adrenoceptor$ antagonists에 의해서 차단되었으나, ${\alpha}_1-adrenoceptor$ antagonist인 prazosin ($10^{-6}M$)에 의해서는 차단되지 않았다. 또한 $Ca^{2+}-free$ medium이나 verapamil($10^{-5}M$)의 전처치에 의해서 이들 agonist의 효과가 완전히 차단되었다. 결론적으로 발정정지기의 돼지 자궁근에서 clonidine, medetomidine, etomidate, xylazine 등은 ${\alpha}_2-adrenoceptors$의 흥분을 통해 자궁근의 수축을 일으키며, 이 효과는 voltage-dependent $Ca^{2+}$ channels을 통한 extracellular $Ca^{2+}$ influx의 증가에 의한 것으로 추론하였다.

• Korean Journal of Veterinary Research
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• v.35 no.4
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• pp.833-841
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• 1995

Ultrasonography was used to measure the corpus luteum area for determining the relationships between corpus luteum size and milk progesterone concentration during the estrous cycle in 16 dairy cows. Cows were classified retrospectively into cows that had corpus luteum with(n=P) and without(n=7) cavity. Ultrasound examination and collection of milk samples for progesterone assay were performed with 2 day intervals from Days 0 to 12, and then daily from Day 14 to the day of the next ovulation. The means for corpus luteum area and for milk progesterone concentration during the estrous cycle were not significantly different between cows that had corpus luteum with and without cavity. The correlation coefficients between corpus luteum area and milk progesterone concentration during luteal development (Days 2 to 8) were 0.71(p<0.0001) and 0.74(p<0.0001) for corpus luteum with and without cavity, respectively, during luteal regression(Days -6 to 0 relative to the next ovulation) 0.73(p<0.0001) and 0.76(p<0.0001), respectively. The correlation coefficients combined fur both stages of estrous cycle and both luteal statuses were 0.70(p<0.0001). These results indicate that corpus luteum area is significantly correlated to milk progesterone concentration, and ultrasonographic assessment of the corpus luteum is a reliable method fur estimating peripheral progesterone concentrations during the estrous cycle in cows.

• Korean Journal of Animal Reproduction
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• v.24 no.1
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• pp.83-88
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• 2000

This study was conducted to determine the effect of repeated ultrasound-guided transvaginal retrieval of oocytes recovery, estrous cycle and ovarian adhesion in Korean native, Hanwoo heifers. Heifers were at unknown stages of the estrous cycle at the start of experiments in which all follicles $\geq$6mm in diameter were ablated. The results obtained in this study were as follows; Follicle developing number and oocytes collected number were no effected to repeated OPU to nine session, 4 e.a range oocytes collected to repeated OPU session. Oocytes were observated follicles were 8.7$\pm$4.2 e.a, collected oocytes were 4.1$\pm$3.4 e.a to two times collected per week and observated follicles was 10.2$\pm$6.1 e.a, collected oocytes were 4.3$\pm$2.9 e.a to one times collected per week, but no difference significantly(P＜0.05). Ovaries adhesive percentage to repeated OPU was eight ovaries adhisived(20%) of forty ovaries, three ovaries(7.5%) to 1~3 times oocytes collected, four ovaries(l0%) to 4~6 times, one ovaries(2.5%) to 7~9 times oocytes collected session. To repeated OPU effection, ovaries adhisive heifers were long estrous cycle(＞25 day) to 7 heads(87.5%) of 8 head, non-adhesive ovaries heifers were 5 heads(41.7%) were long estrous cycle to repeated OPU 12 heads. Although, now unknown about the dynamics of follicles wave and about functional changes to repeated OPU ovaries, more question about ovaries adhesive cause remain.

• Korean Journal of Animal Reproduction
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• v.25 no.3
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• pp.251-257
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• 2001

This study was carried out to investigate the effect of breed, parity, litter size, lactating period, and estrus interval on sow productivity traits in swine. Data from 492 heads of Landrace, Yorkshire or crossbred sow which were raised at Yonam College of Agriculture from March, 1998 to July, 2000 were analyzed for this study. The total number of pigs born (TN), the number of pigs born alive (NB), and the number of pigs suckled (NS) were greater in the crossbred sows than in the purebred. In TN and NB, the 3rd to 7th parities were greater than other parities, and the 8th parity was the lowest. The number of pigs stillbirthed (NSB) and the number of runt per litter tended to increase with the increase of TN. In addition, statistical analysis showed that parity had significant effect on most of traits. The current TN had highly significant effect on TN, NB and NS of the next parity As current TN increased, TN, NB, and NS of the next parity increased. The current lactating period also affected significantly for TN, NB, and NS of the next parity The sows which had the lactating period of 20∼21 days produced the greatest TN and NB in the next parity. Weaning to estrus interval(WEI) had significant effect on TN, NB, and NSB. Among WEI groups, the WEI group of 7∼13 days was the lowest in TN, NB and NSB.

• Journal of Animal Science and Technology
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• v.47 no.6
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• pp.925-936
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• 2005

The present study evaluated whether pentoxifylline added to the freezing extender could improve the sperm characteristics and function in canine frozen semen. Also the conception rate following AI with frozen-thawed semen was investigated. The beneficial effects of pentoxifylline supplementation were visible in motility, viability, acrosome reaction, and tail swelling patterns. Especially, highest sperm viability and function were obtained in the forzen semen supplemented with 1mM pentoxifylline. The follicle size measured by ultrasonography was 6.48 mm, 11.52 mm and 8.9 mm on 11, 13 and 15 days after the onset of natural estrus, respectively and ovulation occurred on 13 and 15 days. The pregnancy rates in bitches inseminated with frozen semen on natural and induced estrus were 71.4% and 75.0%, respectively. There was no significant difference between the pregnancy rates in bitches inseminated with frozen semen following natural and induced estrus, but the litter size was slightly increased in natural cycle.

• Journal of Embryo Transfer
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• v.17 no.3
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• pp.227-234
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• 2002

This study was performed to investigate the improvement of the pregnancy rate of Al or clone embryo transfer on hCG administration in Korean Native heifer. A total of 42 heifers were treated with control, CIDR(withou E2 capsule). hCG after 7 day of hi, the pregnancy rate were spewed 53, 46, 71%. These results were significant different among the treatments(P<0.05). When the hCG were adminstrated at cloned embryo transfer, pregnanacy rate were control, hCG 5.8%, 10.4% respectively and there was no significant different between treatments. Plasma P4 concentration of hCG treatment in heifers were 3 times higher than control on 13~16 day after heat. After this, plasma P4 concentration of CIDR and hCG treated heifers were kept the 2~3 times levels. IGF-I concentration were showed no differences between pregnancy and non-pregnancy. hOG and CIDR. IGF-II concentration were revealed the differences between pregnancy and non-pregnancy in CIDR group but there was no differences in hCG administration group. Plasma cortisol were high at heat and 16 day after heat and CIDR treated group was higher than the other group. These results suggest that hCG administration was improve the pregnancy rates on Al and cloned ET, accompanying the incline of P4 concentration.

• KIPS Transactions on Software and Data Engineering
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• v.2 no.11
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• pp.795-800
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• 2013

Early detection of oestrus in Korean cows is one of the important issues in maximizing the economic benefit. Although various methods have been proposed, we still need to improve the performance of the oestrus detection system. In this paper, we propose a video surveillance system which can detect unusual behavior of multiple cows including the mounting activity. The unusual behavior detection is to detect the dangerous or abnormal situations of cows in video coming in real time from a surveillance camera promptly and correctly. The prototype system for unusual behavior detection gets an input video from a fixed location camera, and uses the motion vector to represent the motion information of cows in video, and finally selects a SVDD (one of the most well-known types of one-class SVM) as a detector by reinterpreting the unusual behavior into an one class decision problem from the practical points of view. The experimental results with the videos obtained from a farm located in Jinju illustrate the efficiency of the proposed method.

• Journal of Embryo Transfer
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• v.22 no.2
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• pp.89-95
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• 2007

The present study was conducted to examine some factors affecting in vitro development and fecundity of embryos recloned with somatic cell nuclear transfer (SCNT). Fibroblast cells retrieved from the ear of a 3-week-old, cloned Korean goat (Jinsoonny) were used as karyoplast donors and serum-starvation was conducted in tissue culture medium (TCM)-199 supplemented with 0.5% FBS. Recipient oocytes were surgically collected by flushing the oviducts 35 h after hCG injection following FSH priming. The zonae pellucidae of the oocytes were partially perforated with a laser drill and a donor cell was transferred into an enucleated oocyte. The couplets were electrically fused and activated by ionomycin (5 min) and 6-DMAP (4 h). The reconstructed embryos were cultured in mSOF medium containing 0.8% BSA at $39^{\circ}C$ in an atmosphere of 5% $CO_2$, 5% $%O_2$, 90% $N_2$ for 12 to 15 h. Re-cloned embryos (2- to 4-cell stages) were surgically transferred into the oviducts of the recipients and pregnancy was subsequently diagnosed by progesterone assay and ultrasound on Days 21 and 63 of pregnancy. The fusion rate following 1st fusion pulse was higher (p<0.05) in 2nd cloning (65.9%) compared to 1st cloning (51.0%), but it was not different in the other groups. The rate of cleavage after fusion was significantly higher (p<0.05) in 1st (77.7%) than in 2nd cloning (56.0%). A total of 175 re-cloned embryos were transferred into 28 recipients. On day 21 and 60 after transfer, 11 (39.3%) and 4 recipients (17.4%) were pregnancy, respectively. In comparison of pregnancy rate by estrous synchronization, a total of 66 and 109 re-cloned embryos were transferred into 11 recipients in natural estrus and 17 recipients in induced estrus, respectively. Five (45.4%) and 2 recipients (18.2%) in natural estrus were pregnant on days 21 and 63 while 6 (35.3%) and 2 (11.8%) recipients in induced estrus were pregnant, respectively. These results show that recloning of goat can be achieved by SCNT and estrous synchronization between donor and recipient animals may be one of the major factors affecting success rate.