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http://dx.doi.org/10.5187/JAST.2005.47.6.925

Studies on Frozen Semen Characteristics Following Pentoxifylline Treatment and Artificial Insemination in Dog  

Ji, D.Y. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University)
Kim, C.K. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University)
Lee, J.H. (National Livestock Research Institute, R.D.A.)
Park, S.J. (National Livestock Research Institute, R.D.A.)
Ryu, L.S. (National Livestock Research Institute, R.D.A.)
Ryu, J.W. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University)
Lee, J.H. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University)
Jeong, Y.C. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University)
Pang, M.G. (Department of Animal Science & Technology and BET Research Institute, Chung-Ang University)
Publication Information
Journal of Animal Science and Technology / v.47, no.6, 2005 , pp. 925-936 More about this Journal
Abstract
The present study evaluated whether pentoxifylline added to the freezing extender could improve the sperm characteristics and function in canine frozen semen. Also the conception rate following AI with frozen-thawed semen was investigated. The beneficial effects of pentoxifylline supplementation were visible in motility, viability, acrosome reaction, and tail swelling patterns. Especially, highest sperm viability and function were obtained in the forzen semen supplemented with 1mM pentoxifylline. The follicle size measured by ultrasonography was 6.48 mm, 11.52 mm and 8.9 mm on 11, 13 and 15 days after the onset of natural estrus, respectively and ovulation occurred on 13 and 15 days. The pregnancy rates in bitches inseminated with frozen semen on natural and induced estrus were 71.4% and 75.0%, respectively. There was no significant difference between the pregnancy rates in bitches inseminated with frozen semen following natural and induced estrus, but the litter size was slightly increased in natural cycle.
Keywords
Pentoxifylline; Dog; CASA; Frozen Semen; AI;
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