• Title/Summary/Keyword: 발모효과

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목초액의 안정성 및 모발 성장 촉진 효과 (Evaluation of Stability in the Purified Wood Vinegar and Its Hair Growth Effect)

  • 조영호;이주연;이종화;조재수;이계원
    • 생명과학회지
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    • 제19권10호
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    • pp.1389-1395
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    • 2009
  • 천연물 신약으로 응용 가능성이 있는 목초액을 산화응집반응과 활성탄 등을 첨가하여 증류하는 방법으로 제조하였으며, 장기보존과 가속 저장 조건에서 목초액의 이화학적 변화(pH, 비중, 굴절률 및 용해 타르), 유해물질(카르보닐기, 벤조피렌 및 잔류용매) 그리고 유기산(개미산, 초산 및 프로피온산)의 변화를 검토하여 안정성을 평가하였다. 또한, 발모 모델로서 C57BL/6 마우스를 이용하여 모발 성장 효과를 살펴보았다. 보관 기간 중에 어떤 화학 결합이 일어나거나 또는 분해반응이 반복적으로 일어나지 않았다. 또한, 유해성분이나 주성분이 일정하게 유지되었고 특히 발암물질인 벤조피렌은 검출되지 않았다. 정제된 목초액 또는 미녹시딜을 2주 동안 등에 도포한 후, 어떤 임상 증상 없이 음성 대조군인 생리식염수보다 모발 성장을 촉진하였다. 따라서 화장품이나 천연물 신약으로 응용가능성이 높은 목초액을 함유한 제품 개발 시 일정한 양으로 유지되는 주성분을 함유하면서 페놀과 같은 유해물질을 줄일 수 있는 방법이 가능할 뿐만 아니라 모발 성장 촉진 효과가 있어 탈모환자에도 유용하게 사용될 것으로 기대되어진다.

전립선비대증의 어성초추출물에 의한 경구투여 효과 (Effect of Oral Administration of Houttuynia Cordata Extract on Benign Prostatic Hyperplasia)

  • 송원영;최정화
    • 생명과학회지
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    • 제29권6호
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    • pp.705-711
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    • 2019
  • 본 연구는 전립선비대증이 노화 및 남성호르몬 과다분비로 인해 유발된다는 가설을 바탕으로 순화기간을 마친 12주령이 된 수컷 쥐에 testosterone을 30일간 피하주사하여 노화 및 testosterone 분비 증가로 인한 전립선비대증 유발 모델 rat을 제작하였다. 실험군은 총 4군으로 각 군당 10마리씩 정상군(N 군), 음성대조군(DO 군), 어성초 추출물 투여군(HO 군) 및 양성물질 finasteride 투여군(FO 군)으로 나누어서 진행하였다. 고환절제술을 한 후 testosterone을 투여한 결과 N군에 비해 나머지 세 군, DO, HO 및 FO군 모두에서 체중이 유의적으로 감소하였다. 전립선의 부피는 N군에 비해서 DO군에서 유의적으로 증가하였으며, HO군과 FO군에서는 DO군에 비해 유의적으로 감소되는 경향을 나타내었다. 전립선의 무게 또한 HO군 및 FO군에서 DO군에 비해 유의적으로 감소되었다. 체중 당 전립선의 비율은 N군에 비해 DO군, HO군 및 FO군에서 유의적으로 증가하였으며, 특히 DO군에 비해 HO 및 FO군은 각각 유의적인 감소를 나타내었다. 혈중 testosterone 및 DHT의 농도는 N군에 비해 모든 군에서 유의적으로 증가되었으나, DO군에서 증가된 혈중 testosterone 및 DHT의 농도는 어성초 추출물 및 finasteride의 공급으로 유의적으로 감소되는 경향이었다. 어성초 추출물의 공급으로 인한 조직병리학적 변화양상을 관찰한 결과 어성초추출물 공급군에서는 DO군에 비해 상피세포, acinar gland, 관상피 세포, 세포핵, 분비선들의 변형 및 위축이 감소하여 정상적인 형태를 나타내고, 양성대조군으로 사용된 finasteride 처리군의 형상과 비슷한 양상을 나타내었다. 어성초 추출물에 대한 이러한 효과는 향후 분자생물학적으로 기전연구들이 더욱 필요하지만 본 결과로 미루어 어성초 추출물 내 함유된 다양한 페놀 및 플라보노이드와 같은 생리활성 성분들은 지금까지 알려져 있던 발모 및 여드름 개선뿐만 아니라 이와 유사한 기전을 가지고 있는 전립선비대증 개선에도 효과적으로 기여할 수 있을 것이라 기대된다.

반하추출물(半夏抽出物)이 자발적 원형탈모 생쥐와 정상 생쥐의 발모효과에 대한 실험적 연구 (Experimental Studies on the Hair Growth Activity of Extracts of Pinelliae Rhizoma in Spontaneous Alopecia Model and Normal C57BL/6N Mice)

  • 정일국;조한영;김태헌;김남석;정한솔;이창현
    • 동의생리병리학회지
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    • 제23권1호
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    • pp.84-92
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    • 2009
  • This experiment was investigated the effects of fresh and ginger processed Pinelliae Rhizoma extracts on hair growth activity, and its fractions(chloroform, ethyl acetate and water fractions) obtained from fresh Pinelliae Rhizoma on hair growth activity of the normal and spontaneous alopecia areata model of C57BL/6N mice for 16 days. The results were as follows: In fresh Pinelliae Rhizoma extracts treated group, hair growth effect was observed in whole skin area(100%) all the normal mice in whose hair had been clipped on 16th days. In ginger processed Pinelliae Rhizoma extracts treated group, hair growth effect was observed in whole skin area in 25% of normal mice in whose hair had been clipped on 16th days. But in control group, hair growth effect was observed in a part of whole skin area in 25% of normal mice. In fresh Pinelliae Rhizoma extracts treated group, hair follicles of middle stage of anagen phase was observed and it were grown down to subcutaneous tissue of skin in all the mice on 10th day. But in ginger processed Pinelliae Rhizoma extracts treated group and control group, Most of hair follicles of telogen phase was observed in skin. The treatment of extracts of fresh Pinelliae Rhizoma increased the expression of TGF-$\beta$(146%), IGF(107%), and prolactin(115%) in the skin of normal C57BL/6N mice compared to control group(100%). But expression of placenta lactogen(93%) was decreased in the skin of normal C57BL/6N mice compared to control group(100%). In spontaneous alopecia model, The hair growth activity of fresh Pinelliae Rhizoma extracts treated group(100%) was observed to be strong compared with the control group(20%) on 15th day. Hair growth activity on chloroform fractions of fresh Pinelliae Rhizoma extracts was observed in whole skin area in 75% of normal mice on the 9th day. In water and ethyl acetate fractions, hair growth activity was observed in a part of whole skin in 75% and 25% of normal mice, respectively. but hair growth activity of control group was not observed. After application of fractions of fresh Pinelliae Rhizoma extracts for 10 days, hair follicles of chloroform fraction treated group was observed middle stage of anagen phase and hair follicle were grown down to subcutaneous tissue of skin in all the mice. But hair follicles of initial stage of anagen phase were observed in water and ethyl acetate fractions. Most of hair follicles of telogen phase was observed in skin of control group. These experiments suggest that extracts of fresh Pinelliae Rhizoma may stimulate the topical hair growth activity and its chloroform fractions can be useful for treatment of alopecia areata.

초등학생의 성교육 효과에 관한 비교연구 (Comparative Study on Effects of Sexuality Education in Elementary School Students)

  • 서순희;홍지영;이무식;나백주;이진용;배석환;유세종
    • 한국산학기술학회:학술대회논문집
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    • 한국산학기술학회 2010년도 추계학술발표논문집 2부
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    • pp.513-516
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    • 2010
  • 본 연구는 성교육이 학생들의 성 지식, 태도, 역할 정체감에 미치는 효과를 알아보기 위해 실험군과 대조군으로 집단을 나누어 실험군에게 의도적인 성교육 실시하여 그 결과를 살펴보고 향후 학교에서 실시되는 성교육 계획 수립에 도움을 주고자 하는 실험연구이다. 본 연구의 대상은 충청북도 제천시에 위치한 J초등학교 5학년 6개 학급을 대상으로 실험군 100(남 50, 여 50), 대조군 100(남 50, 여 50) 총 200명을 대상으로 실시하였으며 자료 수집은 2009년 10월 15일부터 2009년 11월 25일까지 실시하였다. 연구도구는 문헌고찰을 통해 성 지식, 성 태도, 성역할 정체감에 사용한 설문지를 구성타당도 검증을 통해 문항을 구성 선정하였으며, 신뢰도는 Cronbach's ${\alpha}$ 값으로 검증하였다. 연구 대상자의 일반적 특성을 보면 92%의 학생이 부모님이 있고, 88.5%가 형제자매가 있으며, 집안 분위기는 60%가 자유스럽고, 7%가 엄한 것으로 나타났다. 연구 대상자의 성관련 특성으로 가정에서 성교육을 받아본 경험이 있는 경우는 36%로 나타났으며, 성과 관련된 정보를 얻는 순서는 87.5%의 학생이 학교 보건선생님한테 성 관련 정보를 얻는 경우가 대부분이었고, 다음 순서로는 부모님, 서적류, 담임선생님, 영상매체, 친구, 형제자매 순으로 나타났다. 연구 대상자의 신체변화정도를 살펴보면 여자의 경우 월경이 있는 경우는 20%정도, 가슴의 변화는 84%로 나타났으며, 남자의 경우 몽정이 있는 경우는 4%로 나타났으며, 남녀 모두 변성기, 여드름, 발모, 몽정, 월경, 가슴변화 등 한 가지 이상 신체변화가 나타난 경우는 56%로 나타났다. 연구 대상자의 성지식 사전 사후 점수를 비교해보면 두 집단 모두 사전보다 사후 점수가 증가하였다. 사후검사에서 교육을 받지 않은 대조군의 성지식 점수는 소폭 증가하였으나 성교육을 실시한 실험군의 성지식 점수는 더 큰 폭으로 높았으며, 특히 신체발달에서 사전검사에 대조군은(M=2.17), 실험군은 (M=1.66)으로 실험군의 점수가 낮았으나, 사후검사에서는 대조군은(M=2.76), 실험군은(M=3.90)으로 실험군의 점수가 더 높았다. 이상의 결과를 종합해보면 학교에서의 체계화된 성교육은 학생들의 성 지식, 태도, 성 역할 정체감 형성에 도움을 줄뿐만 아니라 성교육을 부끄러워하고, 쑥스러워하며, 어색해 하던 아이들이 문제 중심 학습을 적용하여 자기 주도적으로 문제를 해결하고, 체험, 실습, 역할극, 토의 등의 활동적인 수업에 적극 참여하고, 자연스럽게 활동하여 긍정적인 '밝은 성(性)'으로 변해가는 모습을 관찰할 수 있었다. 성교육은 인간의 성장과 발달에 따라 변화하는 과정이라는 것을 인식하여 건전한 성, 건강한 성을 함양하기 위해 체계적이고 발달 단계에 맞추어 이루어져야 할 것이다.

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자발적인 원형탈모 생쥐(C57BL/6N)와 정상적인 생쥐에서 반모(斑毛)와 골쇄보(骨碎補) 추출 혼합물의 발모(發毛)효과에 대한 실험적 연구 (Experimental Studies on the Hair Growth Activity of Mixed Extracts of Mylabris Phalerata Pall. and Drynariae Rhizoma in Spontaneous Alopecia Model and Normal C57BL/6N Mice)

  • 이문원;조한영;김태헌;김남석;정한솔;이창현
    • 동의생리병리학회지
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    • 제22권4호
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    • pp.778-790
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    • 2008
  • This experiment investigated the effect of mixed extracts obtained from Mylabris phalerata Pall. and Drynariae Rhizoma on hair growth activity of the normal and spontaneous alopecia areata model of C57BL/6N mice for 16 days. First, we examined morphological regrowth of hair in normal and spontaneous alopecia model of C57BL/6N mice. Second, we examined immunoreactive density of vascular endothelial growth factor(VEGF), c-kit and protein kinase $C-{\alpha}(PKC-{\alpha})$ in skin of normal C57BL/6N mice by immunohistochemical methods. Third, we investigated expression of $TGF-{\beta}$, prolactin and placenta lactogen after topical application of mixed extracts of Mylabris phalerata Pall. and Drynariae Rhizoma to skin by RT-PCR. The results were as follows: Hair growth effect from middle and high concentration of mixed extracts of Mylabris phalerata Pall. and Drynariae Rhizoma was observed in 80% of normal mice in whose hair had been clipped in 15th days. Hair growth effect of all concentrations of mixed extracts of Mylabris phalerata Pall. and Drynariae Rhizoma was observed in 100% of spontaneous alopecia model of C57BL/6N mice in 15th days. Immunoreactive density of VEGF, c-kit and $PKC-{\alpha}$ in skin of all concentrations of mixed extracts of Mylabris phalerata Pall. and Drynariae Rhizoma were strongly stained in epidermis, bulge, secondary hair germ cells and cutaneous trunci m. compare to control group in 10th day. In experimental III group, Immunoreactive density of VEGF, c-kit and $PKC-{\alpha}$ in skin were strongly stained in inner and outer root sheath of skin. The treatment of mixed extracts of Mylabris phalerata Pall. and Drynariae Rhizoma increased the expression of $TGF-{\beta}$, placenta lactogen and prolactin in the skin of normal C57BL/6N mice compared to control group. These experiments suggest that mixed extracts of Mylabris phalerata Pall. and Drynariae Rhizoma may stimulate the topical hair growth activity and it can be useful for treatment of alopecia areata.

삼황사심탕의 항산화능 및 C57BL/6 마우스 모델에서의 발모 촉진효과 (Antioxidant Ability and Hair Growth Effect of Samhwang-Sasimtang in C57BL/6 Model)

  • 김민지;박교현;이인철;김배환
    • 동의생리병리학회지
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    • 제28권2호
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    • pp.154-161
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    • 2014
  • The purpose of this study is to evaluate the antioxidant activity and hair growth effects of a natural herbal ethanol extract, Samhwang-Sasimtang(SS). In case of antioxidant ability of SS, the content of phenolic compounds was 28.44mg/g. The extract showed strong electron donating ability and free radical scavenging activity in a concentration-dependent manner. SOD-like activity also rose through increasing the concentrations of SS. In order to estimate the hair growth effects, the extract was applied to the back of seven-week-old C57BL/6 male mice ($150{\mu}{\ell}$ a day, five days a week, for four weeks) in four groups (C, control, saline; PC, positive control, 3% minoxidil; E1, experimental 1, 1% SS; E2, experimental 2, 2% SS). Ten mice were assigned to each group and five mice in each group were sacrificed at weeks 2 and 4, respectively. There was no a significant difference in body weight change among experimental groups. In macroscopic observation of hair growth at week 4, the scores of hair growth on the backs of mice were 80, 60, 40 and 20% in the PC, E2, E1 and C groups. In terms of histological observation, the ratio and thickness of hair follicles, the enzyme activities of ALP and ${\gamma}$-GT, immunohistological examination of IGF-1 and VEGF which are the hair growth factors, in each group were significantly high in this order: PC, E2, E1 and C group, at week 4. Meanwhile, hair growth inhibition factors, TGF-${\beta}1$ and Caspase-3, were reduced in PC, E1 and E2 groups compared with C group. These results indicate that SS extract may be effective in promoting hair growth, and suggest that it can be used practically as a superior natural agent for hair growth promotion.

C57BL/6N 생쥐에서 골쇄보(骨碎補) 추출물을 이용한 발모(發毛)효과에 대한 실험적 연구 (Experimental Studies on the Hair Growth Activity of Drynariae Rhizoma Extracts in C57BL/6N Mice)

  • 정석영;정일국;김대근;조한영;정한솔;이창현
    • 동의생리병리학회지
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    • 제21권2호
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    • pp.453-461
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    • 2007
  • Drynariae Rhizoma has been used for promotes mending of the sinews and bone, tonifies the kidney for such symptoms as weak low back and knees, and stimulates the growth of hair as a tinctute for alopecia in oriental medicine. This experiment examined the effect of an acetone extracts of Drynariae Rhizomas(GSB-1), its EtoAc fraction(GB-2) and n-buOH fraction(GSB-3), on hair growth activity of the C57BL/6L mice after topical application to skin. First, We examined on hair growth activity of extracts of Drynariae Rhizomas compare to control and 1 % minoxidil groups. Second, We investigated on the number of hair follicle and mast cells after topical application of extracts of the Drynariae Rhizomas to skin for 16 day. Third, We investigated immunoreactive density of vascular endothelial growth factor(VEGF), protein kinase C-${\alpha}$(PKC-${\alpha}$) and stem(mast) cell factor(SCF) in skin of C57BL/6N mice by immunohistochemical methods. The results were as follows : Hair growth effect of acetone extracts of Drynariae Rhizomas, its EtoAc fraction and n-BuOH fraction was observed in 98 %, 96 % and 60 % in hair removed skin area in 16 day respectively, Immunoreactive density of VEGF in skin of GSB-1 group was weakly stained compare to control group in 10 day, But GSB-2 and GSB-3 groups were mildy stained in bulge and root sheath of skin. Immunolocalization of SCF antigens was observed weakly stained density in epidermis, bulge, stem cells and dermal papilla of control gruop. but in experimental group, immunoreactivity of SCF antigens was observed mildly stained density in bulge, epidermis and root sheath of GSB-1 gruop, heavily stained density in epidermis, bulge and root sheath of GSB-2 and GSB-3 groups to the hair removal skin of C57BL/6N mice on day 10. These experiment suggest that acetone extracts of Drynariae Rhizomas and its EtoAc fraction may be used for topical treatment of alopecia areata.

C57BL/6N 생쥐에서 반모추출물과 분획물을 이용한 발모(發毛)효과에 대한 실험적 연구 (EXperimental Studies on the Hair Growth Activity of Mylabris Phalerata Pall. Extracts and Fractions in C57BL/6N Mice)

  • 조한영;김태헌;김호;정한솔;이창현;이광규
    • 동의생리병리학회지
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    • 제22권2호
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    • pp.357-364
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    • 2008
  • Mylabris is the dried body of the chinese blister beetle. The species used in medicine are Mylabris phalerata and M. cichorii. In recent studies, it has been found that Mylabris possesses antitumor properties, increases the number of leukocytes, and has irritant effects on the urinary organs. The crude extracts of Mylabris have been noted for their highly irritant action and other traditional uses of Mylabris include treatment of poor local blood circulation. The active constituent of Mylabris is cantharidin. The chemical is notable for its vesicant properties, but with severe side effects such as nephrotoxicity. This experiment examined the effect of extracts and fractions, obtained from Mylabris phalerata Pall. on hair growth activity of the C57BL/6N mice after topical application to skin. First, we examined the effect of an extracts, obtained from the alcohol extracts of dried Mylabris phalerata Pall. on hair growth activity of the C57BL/6N mice after topical application to skin. Second, we examined on hair growth activity of the cantharidin fraction of Mylabris phalerata Pall. compared to the control and 1% minoxidil groups. Third, we investigated the number of hair follicle and mast cells after topical application of extracts of Mylabris phalerata Pall. to skin for 16 days. The results were as follows: Hair growth effect from the extracts of Mylabris phalerata Pall.(0.312%) was observed in 80% of mice whose hair had been removed in 13 days. Hair growth effect from the extract of Mylabris phalerata Pall.(0.312 and 0.625%) and 1% minoxidil group was observed in 100% of mice whose hair had been clipped in 20 days. Hair growth effect from the cantharidin fraction(0.5%) and water fraction(0.5%) of Mylabris phalerata Pall. was observed in 100% of mice whose hair had been clipped in 24 days. The hair growth effect on the cantharidin fraction(0.125%) was observed to be strong compared with the minoxidil(3%) group, commercial hair growth agents, in mice whose hair had been clipped in 19 days. In the spontaneous alopecia mice model, the hair growth effect from the cantharidin fraction (0.125%) was observed to be strong as compared with the states before the 13 days experiment. These experiments suggest that extracts and fractions of Mylabris phalerata Pall. may stimulate the topical hair growth activity in low doses.