• Korean Journal of School Psychology
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• v.17 no.2
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• pp.199-222
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• 2020

This study was conducted to verify the effects of socially prescribed perfectionism on depression as mediated by intolerance of uncertainty and either ruminating or reflective response styles, on as will as to find ways to effectively address depression as it presents in university students. For this purpose, 283 university students at the central region were assesed using data collected from the following surveys: the Center for Epidemiological Studies-Depression Scale(CES-D), the Multidimensional Perfectionism Scale(HMPS), the Intolerance of Uncertainty Scale(IUS), and the Response to Depressed Mood Questionnaire(RDQ) and that were used to collect data. The results of this study are as follows. First, there was a positive correlation between the Intolerance of uncertainty and the rumination response style to the uncertainty of socially prescribed perfectionism, but not correlation the reflective response mode. Second, with respect to the relationship between socially prescribed perfectionism and depression, intolerance of uncertainty was set as the primary mediator variable and the rumination response style were set as the secondary mediator variables. The results shown to have a significant mediating effects. However this mediating effect was not significant when, in order to verify the mediating effect, we set the secondary mediator variable as the reflective response style. These results suggest that to prevent depression that manifests as socially prescribed perfectionism, it may be effective to intervene with a focus on the intolerance of uncertainty and the rumination response style. Finally, the implications and limitations of the study are discussed based on the results.

• The Journal of the Korea Contents Association
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• v.17 no.11
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• pp.479-490
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• 2017

The study analyzed the relationship between dating violence and limerence and ruminative response in romentic relationship. The subjects were 205 college students who had experience of dating. And mean age of subjects was 22.1 years. Analysis methods were correlation analysis, ANOVA, two-step cluster analysis, and multinomial logistic regression analysis. The results of this study are as follows. First, self-reproach ruminative respone were significantly higher the victim group and perpetrator victim group than the general group. Second, all sub-factors of ruminative respone were significantly higher the victim group and perpetrator victim group than the general group. Third, the self-reproach ruminative respone was significant positive explanatory variable on dating violence. Fifth, the victim limerence experience significantly increased the odds ratio of victim group of dating violence by 3.3 times, and that of perpetrator victim group of dating violence by 10.9 times. Based on these findings, he discussed the importance of dating violence and the importance of limerence and rumination.

• 월간낙농육우
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• s.109
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• pp.69-76
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• 1991

젖소는 반추동물이기 때문에 효과적인 사료급여 프로그램을 짜는데 어려움이 많다. 제1위(반추위)는 사람의 실수로 부적합하게 만들어진 사양프로그램의 문제를 완화시켜 주기도 하는 반면 정량적인 예측의 정확성을 기하는데 많은 어려움을 제공하기도 한다. 다시말해서 우리는 정확한 정보가 부족한 상황에서 영양의 요구량을 계산하거나 사료급여 프로그램을 짜고 있는 것이다. 따라서 영양소의 이용과 우유 생산반응을 논할때는 이러한 정보의 부족을 염두에 둘 필요가 있다.

• Journal of Life Science
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• v.28 no.10
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• pp.1244-1253
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• 2018

Rumen microbiome consists of a wide variety of microorganisms, such as bacteria, archaea, protozoa, fungi, and viruses, that are in a symbiotic relationship in a strict anaerobic environment in the rumen. These rumen microbiome, a vital maker, play a significant role in feed fermentation within the rumen and produce different volatile fatty acids (VFAs). VFAs are essential for energy metabolism and protein synthesis of the host animal, even though emission of methane gas after feed fermentation is considered a negative indicator of loss of dietary energy of the host animal. To improve rumen microbial efficiency, a variety of approaches, such as feed formulation, the addition of natural feed additives, dietary feed-microbes, etc., have taken to increase ruminant performance. Recently with the application of high-throughput sequencing or next-generation sequencing technologies, especially for metagenomics and metatranscriptomics of rumen microbiomes, our understanding of rumen microbial diversity and function has significantly increased. The metaproteome and metabolome provide deeper insights into the complicated microbial network of the rumen ecosystem and its response to different ruminant diets to improve efficiency in animal production. This review summarized some recent advances of rumen microbiome techniques, especially "meta-omics," viz. metagenomic, metatranscriptomic, metaproteomic, and metabolomic techniques to increase feed fermentation and utilization in ruminants.

• Journal of Life Science
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• v.31 no.5
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• pp.520-526
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• 2021

Microbial protein is one of the sources of protein in the rumen and can also be the source of glutamate production. Glutamic acid is used as fuel in the metabolic reaction in the body and the synthesis of all proteins for muscle and other cell components, and it is essential for proper immune function. Moreover, it is used as a surfactant, buffer, chelating agent, flavor enhancer, and culture medium, as well as in agriculture for such things as growth supplements. Glutamic acid is a substrate in the bioproduction of gamma-aminobutyric acid (GABA). This review provides insights into the role of glutamic acid and glutamic acid-producing microorganisms that contain the glutamate decarboxylase gene. These glutamic acid-producing microorganisms could be used in producing GABA, which has been known to regulate body temperature, increase DM intake and milk production, and improve milk composition. Most of these glutamic acid and GABA-producing microorganisms are lactic acid-producing bacteria (LAB), such as the Lactococcus, Lactobacillus, Enterococcus, and Streptococcus species. Through GABA synthesis, succinate can be produced. With the help of succinate dehydrogenase, propionate, and other metabolites can be produced from succinate. Furthermore, clostridia, such as Clostridium tetanomorphum and anaerobic micrococci, ferment glutamate and form acetate and butyrate during fermentation. Propionate and other metabolites can provide energy through conversion to blood glucose in the liver that is needed for the mammary system to produce lactose and live weight gain. Hence, health status and growth rates in ruminants can be improved through the use of these glutamic acid and/or GABA-producing microorganisms.

• Microbiology and Biotechnology Letters
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• v.34 no.1
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• pp.35-39
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• 2006

One of the fermentative metabolism of enteric Escherichia coli was imitated after rumen bacteria, which have high C4 metabolism. E. coli expresses phosphenolpyruvate carboxylase (PPC) for the pathway between phosphoenolpyruvate (PEP) and oxaloacetate (OAA) during glycolytic condition while expresses phosphoenolpyruvate carboxykinase (PCK) during gluconeogenic condition. In contrast to enteric E. coli, rumen bacteria express the PEP-OAA pathway only by PCK. To verify the effect of the regulation imitation on the C4 metabolism of E. coli, PPC-deficient E. coli strain with PCK expression in glycolytic condition was constructed. The PEP-OAA regulation modified E. coli strain increased 2.5-folds higher C4 metabolite than the wild type strain. The potential use of C4 metabolism by regulation control is discussed.

• Journal of Animal Science and Technology
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• v.49 no.6
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• pp.819-828
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• 2007

Two experiments were conducted to observe the effects of direct fed microbials on metabolic characteristics in sheep and milking performance in dairy cows. A metabolic trial with four ruminally cannulated sheep(60±6kg) was conducted in a 4×4 Latin square design to investigate the supplementation effects of Saccharomyces cerevisiae, Clostridium butyricum or mixed microbes of S. cerevisiae and C. butyricum on ruminal fermentation characteristics and whole tract digestibility. Sheep were fed 1.25 kg of total mixed ration(TMR, DM basis) supplemented with S. cerevisiae (2.5g/day), C. butyricum (1.0g/day) or its mixture(S. cerevisiae 1.25g/day+C. butyricum 1g/day), twice daily in an equal volume. But control sheep were fed only TMR. A feeding trial with 28 lactating Holstein cattle was also conducted for 12 weeks to investigate the effects of the same microbial supplements as for the metabolic trial on milking performance. The cows were fed the TMR(control), and fed S. cerevisiae(50g/day), C. butyricum(15g/day) or its mixture (S. cerevisiae 25g/day + C. butyricum 7.5g/day) with upper layer dressing method. Total VFA concentration and the digestibility of whole digestive tract in the sheep increased by supplementation of S. cerevisiae, C. butyricum or their combined microbials compare to control group. The proportion of propionic acid at 1h(P<0.039) and 3h(P<0.022) decreased by supplementation of S. cerevisiae while tended to increase acetic acid proportion at the same times. Daily dry matter intake(DMI) was not influenced by the microbial treatments, but milk yield(P<0.031) and feed efficiency(milk yield/DMI, P<0.043) were higher for the cow received C. butyricum than those for other treatments. The milk fat content was higher (P<0.085) when cows fed S. cerevisiae(4.11%) than that fed the control (4.08%), the diets with C. butyricum (3.85%) and the microbial mixture. Based on the results obtained from the current experiments, supplementation of C. butyricum or mixture with S. cerevisiae might be increased milk fat content and milk productivity of lactating daily cows. (Key words:Saccharomyces cerevisiae, Clostridium butyricum, Fermentation characteristics,

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.6
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• pp.1086-1093
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• 2012

This research was carried out to develop the correction method of VDI4630 method improving accuracy, and investigated the effects of carbonate ion ($CO_3{^{2-}}$) and reactant water ($H_2O$) on anaerobic organic biodegradability in VDI4630 method. Pig blood, pig intestine residue, pig digestive tract content, and cattle rumen content were experimented as waste biomasses. Chemical formulas of pig blood, pig intestine residue, pig digestive tract content, and cattle rumen content were $C_{3.78}H_{8.39}O_{1.46}N_1S_{0.01}$, $C_{9.69}H_{15.42}O_{2.85}N_1S_{0.03}$, $C_{25.17}H_{43.32}O_{15.04}N_1$, $C_{27.23}H_{42.38}O_{15.93}N_1S_{0.11}$, respectively. And amount of reactant moisture for the anaerobic degradation of organic materials were 0.336, 0.485, 0.227, 0.266 mol, respectively. In pig blood, pig intestine residue, pig digestive tract content, and cattle rumen content, anaerobic organic biodegradability presented as $B_u/B_{th}$ were 82.3, 81.5, 70.8, and 66.1%, and anaerobic organic biodegradability (AB) by VDI4630 method were 72.2, 87.8, 74.2, 62.0%, and that were significantly different with anaerobic organic biodegradability presented as $B_u/B_{th}$. The effects of carbonate ion and reactant water on anaerobic organic biodegradability were not significant, But Accuracy of anaerobic organic degradability was expected to able to be improved by the correction method of VDI4630 considering the carbonate ion at digestate and the reactant water quantified.

• Therapeutic Science for Rehabilitation
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• v.9 no.2
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• pp.73-86
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• 2020

Objective : The purpose of this study was to examine the effects of depressive symptoms on the relationship between avoidance(brooding, reflection, and behavioral avoidance) and sleep quality. Methods : In total, there were 947 subjects who were assessed using self-reports on three measures: Center for Epidemiological Studies-Depression (CES-D), Ruminative Response Style (RRS), Cognitive-Behavioral Avoidance Scale (CBAS), and Pittsburgh Sleep Quality Assessment (PSQI). Results : First, depressive symptoms fully mediated the relationship between brooding and sleep quality. Second, reflection did not mediate the relationship between brooding and sleep quality, and reflection had no direct effects on depressive symptoms. Third, depressive symptoms partially mediated the relationship between behavioral avoidance and sleep quality. Conclusion : This study was confirmed the pathological characteristics of brooding compare to reflection, and the essential role of behavioral avoidance and depressive symptoms on sleep problems.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.1
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• pp.58-66
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• 2013

This study was conducted in order to develop slaughterhouse rumen content (SRC) as a potential feed additive. The moisture content of SRC can reach 80%, and therefore an appropriate dewatering process is required before it can be used. In this study, the effects of heating temperature, heating time, and filler content during the dewatering process on the activity of various enzymes in SRC were investigated. The Box-Behnken experimental design was employed, involving a total of 45 experimental runs, consisting of three variables (heating time, heating temperature, and filler content) with three levels per variable (12, 30 and 48 hr; 60, 75 and $90^{\circ}C$; 12, 22.5 and 33% for heating time, heating temperature, and filler content, respectively). For enzyme activities, xylanase, cellulase, and amylase were examined, and the results were subjected to an analysis of variance. Heating time, heating temperature and filler content had significant effects on the activity of each enzyme (p<0.05). Cellulase and amylase activities decreased (p<0.05) at elevated heating temperatures, whereas xylanase was reasonably stable around $90^{\circ}C$. The activities of all enzymes decreased (p<0.05) with increased heating time. Optimum filler contents for xylanase, cellulase, and amylase activities were 22.5, 12 and 33%, respectively. However, optimum conditions for all variables that simultaneously maximize the activity of all three enzymes could not be ascertained in this study. Nevertheless, the results from the current study can be useful as basic information for the development of SRC as a feed additive enriched with improved major enzymes for livestock feed digestion.