• Title/Summary/Keyword: 반응도(R)

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Glucose Determination by Using Korean Radish Anionic Peroxidase (한국산 무 (Raphanus sativus L.) anionic peroxidase를 이용한 당 정량법 연구)

  • Kim, Jae-Hong;Kim, Sung-Ho;Lee, Mi-Young
    • Applied Biological Chemistry
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    • v.43 no.2
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    • pp.100-105
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    • 2000
  • Anionic peroxidases (POD) were isolated from Korean radish (Raphanus sativus L.) root by using fractionation with $(NH_4)_2SO_4$ and CM-cellulose ion exchange chromatography and used as the colorimetric enzyme for glucose determination. The chromogen used in this work was o-tolidine or 4-aminoantipyrine/diethylaniline (4AA/DEA) and the colored products were measured at 630 nm. Korean radish anionic POD showed much better colorimetric reaction of glucose determination with 4AA/DEA than with o-tolidine. The r values of calibration curve for glucose determination by o-tolidine and 4AA/DEA were 0.9983 and 0.9963, respectively. In order to compare the reactivity for substrate oxidation by Korean radish POD and horseradish POD, the Km values against o-dianisidine and guaiacol were measured. Korean radish POD had about 40 fold higher affinity for o-dianisidine and 2 fold higher affinity for guaiacol as revealed by Km values. These results showed that Korean radish POD could be developed as the colorimetric diagnosis reagent for glucose determination with high sensitivity.

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Optimization of Extraction Conditions for Ethanol Extracts from Citrus unshiu Peel by Response Surface Methodology (반응표면분석에 의한 진피 에탄올 추출물의 추출조건 최적화)

  • Jeong, Ji-Eun;Shim, Sang-Phil;Jeong, Yoo-Seok;Jung, Hee-Kyoung;Kim, Young-Chan;Hong, Joo-Heon
    • Food Science and Preservation
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    • v.18 no.5
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    • pp.755-763
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    • 2011
  • This study was conducted to monitor the quality characteristics Citrus unshiu ethanolic extracts by a response surface methodology. In extraction conditions based on the central composite design with variations in ethanol concentration (0~100%), extraction temperature($35\sim95^{\circ}C$), and ratio of solvent to sample(20 mL/g). The maximum value of yield was 36.31% at $93.18^{\circ}C$, 67.13% of ethanol concentration. The extraction yield and total polyphenol content were improved with the increase of ethanol concentration than extraction temperature. Total flavonoid content was improved with the increase of extraction temperature than ethanol concentration. The coefficients of determinations($R^2$) were 0.8646(p<0.1) and 0.9153(p<0.05) in electron donating ability and hesperidin content, respectively. Estimated conditions for the maximized extraction including yield, total polyphenol content, total flavonoid content, electron donating ability, and hesperidin content were 55~73% in ethanol concentration, $89\sim95^{\circ}C$ in extraction temperature, and 20 mL/g in ratio of solvent to sample.

Monitoring of the Optimum Conditions for the Fermentation of Onion Wine (양파주의 최적 발효조건 모니터링)

  • Choi, In-Hag;Jo, Deokjo;Lee, Gee-Dong
    • Food Science and Preservation
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    • v.20 no.2
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    • pp.257-264
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    • 2013
  • Central composite design along with response surface methodology (RSM) was applied to improve the fermentation process in onion (Allium cepa) wine production. The effects of different fermentation parameters (time, temperature, and initial sugar content) were found to be significant with respect to the physicochemical and sensory properties of wine. The maximum score for the alcoholic content was obtained at $29.27^{\circ}C$ fermentation temperature, 103.43 h fermentation time, and $27.52^{\circ}Brix$ initial sugar content. The maximum score for overall palatability was obtained at $39.27^{\circ}C$ fermentation temperature, 57.28 h fermentation time, and $22.14^{\circ}Brix$ initial sugar content. The coefficients of determination ($R^2$) were 0.9620 and 0.9060 for alcoholic content and overall palatability, respectively. The ranges of the optimum fermentation conditions ($28{\sim}32^{\circ}C$, 80~90 hr, and $20{\sim}25^{\circ}Brix$) were obtained by superimposing the response surfaces with regard to the alcoholic content and overall palatability of onion wine.

UV Effects on Production and Photoreactivity of Chromophoric Dissolved Organic Matter in Media of Polar Marine Phytoplanktons (극지 식물플랑크톤의 유색 용존 유기물의 생산과 광반응성에 대한 자외선 영향)

  • Park, Mi Ok;Ha, Sun-Yong
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.28 no.5
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    • pp.712-720
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    • 2022
  • In this study, we evaluated the production and photoreactivity of CDOM of two polar phytoplanktons - Phaeocystis antarctica and Phaeocystis pouchetii, in order to find out UV effects on phytoplanktons. In visible region, CDOM in media of both phytoplanktons under UV-R decreased during 48hrs incubation period. However, in UV region CDOM decreased 30 % in the media of P. antarctica, but increased 10% in media of P. pouchetii, compared to CDOM concentrations of control after 48 hr incubation. This result indicates that biota in polar environment would not well protected from UV-R harmful effect when P. antarctica is dominant because of loss of CDOM, but when P. pouchetii is dominant species, production of UV absorbing organic matter could play more efficiently for UV screening for marine biota. Also we confirmed that FDOM of humic substance (C-peak) produced by these phtoplanktons under UV-R stress were well matched with fluorescence characteristics of the UV-protecting compound, MAAs. This finding shows that Phaeocystis pouchetti with low photoreactivity would contribute to DOM pool of polar marine environment under stratification by global warming.

The Relationship Between The Type of R&D Investment and a Firm's Performance (연구개발 투자성향과 기업성과의 관계)

  • Kim, Kyung-Ihl
    • Journal of Convergence for Information Technology
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    • v.8 no.4
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    • pp.213-217
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    • 2018
  • The relationship between R&D investment and subsequent change has been mostly confirmed under additional influencing factors, with the form of innovation investments. The research assumes that a firm adjusts its R&D spending in accordance to performance feedback. It is argued that an increased fluctuation of a firm's R&D expense is related to a reduced performance. This hypothesis is tested on SME in World class 300 Projet by SMBA. Using panel data models, instability measured by SD is related to performance levels measured by ROA, ROE & PM. Results support the proposed relationship between R&D expense instability and the subsequent performance. Although a causal link cannot be clearly established, the results indicate that firms with a lower performance have higher R&D investment fluctuations, possibly being more responsive to performance feedback.

Electrochemical Properties of Cu(I)hexafluoroacetylacetonate (Cu(I)hexafluoroacetylacetonate 착화합물들의 전기화학적 성질)

  • Choi, Yong-Kook;Jeong, Byeong-Goo;Shin, Hyun-Kook
    • Journal of the Korean Chemical Society
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    • v.37 no.9
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    • pp.806-812
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    • 1993
  • Complexes of $Cu(I)(hfac)PR_3$(P: phosphine and R: Me, Et and Bu) as Cu(I)(${\beta}$-diketonate) compounds have been synthesized and their electrochemical properties have been investigated using glassy carbon and carbon microelectrode in aprotic solvent. Reduction process of $Cu(I)(hfac)PR_3$ compounds carried out one electron pathway to Cu(0) by cyclic voltammetry in acetonitrile solution. Chronoamperometric curve using carbon microelectrode shows that these complexes are one electron process and diffusion coefficients are $4.5{\sim}6.7{\times}10^{-6}cm^2$/sec.

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A Study on Public Relations Strategy and Program on Geo-Technology R&D Outcome (지질자원기술 연구개발성과에 대한 홍보 전략 및 방안 연구)

  • Kim, Chan-Souk;Kim, Seong-Yong;Lee, Jae-Rock
    • Economic and Environmental Geology
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    • v.40 no.6
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    • pp.797-804
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    • 2007
  • This study was carried out to analyze public recognition of geo-technology and its outcome. On the basis of the study, the task of PR/communication to promote status of geo-technology and the PR program by stage(SMTCR) for strategy of geo-technology outcome were suggested as follows; operation of PR committee and plus-one appraisal system on a sender scope, media caravan tour and opinion editorial for geo-technology on a message scope, geoscience award and active use of "the year of earth science" on a tool and channel scopes, and regular measurement of public response and regular appraisal of PR goal/results on a receiver scope.

Ionic Hydrogenation of Carbonyl Groups With Molybdenum and Tungsten Complexes (몰리브덴과 텅스텐 착물을 이용한 카르보닐기의 이온성 수소화 반응)

  • Song Jeong-Sup
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.7 no.4
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    • pp.715-720
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    • 2006
  • Metalhydrides such as $Cp(CO)_{2}(L)MH$ (L = t-butylisocyanide and 2,6-dimethylphenylisocyanide M = Mo and W) have been synthesized and used for ionic hydrogenation of the carbonyl groups in the presence of triflic acid. When these complexes have also used as catalyst precursors for hydrogenation of 3-pentanone under mild conditions ($23^{\circ}C,\;<4.1\;atm H_{2}$). The turnover rates were very slow, with the fastest initial rate of about 2 turnovers per 1 day for the [$Cp(CO)_{2}(ArNC)Mo][BA_{r}^{F}_{4}$] system.

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Night Soil Treatment by Anaerobic Sequencing Batch Reactor (혐기성 연속 회분식 반응조에 의한 분뇨처리)

  • 허준무;박종안
    • Journal of environmental and Sanitary engineering
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    • v.15 no.2
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    • pp.75-84
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    • 2000
  • 운전 온도 $35^{\circ}C$, 평균 유기물부하 $3.1{\;}kgCOD/m^3/day$ 및 수리학적체류시간 10일에서 혐기성 연속회분식공정에 의한 분뇨처리를 수행하였다. 공정의 평가는 대조 소화조로 완전혼합형의 소화조와 병행하여 수행되었다. 본 실험에서 분뇨는 고농도의 암모니아성 질소와 침전성 고형물을 함유하고 있음에도 불구하고 희석 없이 소화가 가능하였다. 혐기성 연속회분식공정에서 고형물은 급속하게 증가하여 완전혼합형의 대조 소화조에 비하여 소화조내 고형물(biomass)의 농도가 2.4배로 증가하였고, 가스발생량에 있어서도 대조 소화조에 비해 현격한 증가를 보였으며 그 증가율은 205~220%에 달했다. 부가적인 침전 시설이 없이도 혐기성 연속회분식공정의 유출수질이 대조 소화조 보다 높게 나타났는데 상징액 기준으로 휘발성고형물 제거율은 혐기성 연속회분식공정이 대조 소화조 보다 12~14% 높았다. 한편, 혐기성 연속회분식공정의 운전인자로 반응/침강비(R/T ratio)를 조사한 결과 R/T비가 1인 경우가 3의 경우보다 가스발생량, 메탄함량 및 유기물 제거율이 약간 높았으나 큰 차이는 없었다. 위의 실험결과들로부터 혐기성 연속회분식공정은 고농도의 암모니아성 질소와 침전성 유기물을 함유하고 있는 분뇨의 처리에 효과적이고 안정적인 공정으로 판단된다.

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Screening Evaluation and Predicting Prognosis of Craniomandibular Disorder Patients with the Solberg Questionnaire (Solberg 설문지를 이용한 두개하악장애환자의 간이평가 및 예후예측)

  • Mi-Hi Park;Myung-Yun Ko
    • Journal of Oral Medicine and Pain
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    • v.19 no.2
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    • pp.111-123
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    • 1994
  • 저자는 1990년부터 1993년 사이에 부산대학병원 구강내과에 내원하여 두 개하악장애로 진단되어 보존적 치료를 시행받은 884명의 환자를 대상으로 Solberg의 악관절장애조사 설문지를 작성케한 후, 성, 연령, 병력기간, 진단명, SCL-90-R, 치료에 대한 반응에 따라 환자군을 세분하여 각 환자군의 설문지 문항별 응답양태 및 이에 따른 예후예측을 분석한 바 다음과 같은 결론을 얻었다. 1. 악기능 및 예후악화요인 문항에서는 여성이 높은 응답수를 보인 반면, 기여요인 및 습관문항에 서는 남성이 높은 응답수를 나타내었다. 2. 고령층의 환자에서 행동 및 예후악화요인이 두드러졌다. 3. 만성군이 급성군에 비해 전 문항에서 높은 응답수를 나타내었다. 4. 혼합군 및 근육장애혼자가 관절장애환자에 비해 설문지 전 문항에서 많은 응답을 하였다. 5. SCL-90-R에서 비정상인 환자가 정상군의 환자에 비해 악기능을 제외한 전 문항에서 높은 응답수를 보였다. 6. 치료에 무반응인 환자가 성공한 환자에 비해 습관요인 문항을 제외한 설문지 전체에서 많은 응답을 하였다.

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