• Applied Microscopy
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• v.1 no.1
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• pp.35-42
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• 1969

가토간장에서 채취(採取)한 간(肝)디스토마 성충(成蟲)의 정소(精巢)와 수정낭(受精囊)을 1.25% glutaraldehyde와 1% 사산화(四酸化) 오스뮴산(酸)으로 냉실(冷室)에서 이중고정(二重固定)하였다. 고정(固定)된 시료(試料)는 양식(樣式)에 따라 탈수(脫水)한 후(後) Epon-812로 포매(包埋)하여 MT-2형(型) Porter Blum microtome으로서 초박절편(超薄切片)을 만들어 수사화연(水酸化鉛)과 초산(醋酸)우라닐로서 이중염색(二重染色)한후 Hitachi HS-7형(型)과 HU-11E형(型)의 전자현미경(電子顯微鏡)으로 관찰(觀察)하였다. 관찰결과 간(肝)디스토마의 정세포(精細胞)는 타원형으로 난형(卵形)의 인(仁)을 포함(包含)한 큰 핵(核)을 갖고 있으며 핵(核)을 둘러싼 비교적소량(比較的小量)의 세포질(細胞質)에는 낭형(囊形)의 조면소포체(粗面小胞體)가 희소(稀少)하게 있으며 유리(遊離)리보좀 및 즐(櫛)을 가진 미토콘드리아, 중심체(中心體), 층판상(層板狀)의 골지체가 있다. 정자(精子)는 긴 원주형(圓柱形)의 핵(核)과 선단(先端)에 첨체(尖體)를 포함(包含)한 두부(頭部)와 중종편(中終片), 미부(尾部)의 말단부(末端部)로 갈수록 점차 가늘어져서 결국(結局) 편모(鞭毛)로 끝난다. 두부(頭部)의 선단부(先端部) 가까이 핵환(核環)이 있으므로 이를 기시점(起始點)으로 직경(直徑) $250{\AA}$ 정도의 $8{\sim}10$개(個)의 미세소관(微細小管)이 축사(軸絲)의 배면(背面) 원형질막(原形質膜) 직내면(直內面)의 외형질(外形質)에 평행(平行)하게 중종편(中終片)까지 신장(伸長)되어 있다. 미토콘드리아는 융합(融合)되어 두부(頭部)의 후반부(後半部) 핵(核)의 복측(腹側)에 평행(平行)하게 축사(軸絲)를 감싸는 원추형(圓錐形)으로 종단면(縱斷面)에서 반점상(班點狀)의 횡문(橫紋)이 관찰(觀察)되었다. 중심체(中心體)에서 기원(起原)된 축사(軸絲)는 중심부(中心部)에 중심섬유(中心纖維)와 중심초 및 이중(二重)의 미세소관(微細小管)이 9개(個) 둘러싸고 있는 구조(構造)를 하고 있다.

• Applied Microscopy
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• v.33 no.4
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• pp.267-274
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• 2003

The ultrastructures of spermatogenesis and sperm in Xiphophorus maculatus, ovoviviparous fish were investigated by electronmicroscopy The testis of Xiphophorus maculatus contained numerous testicular sacs, and spermatogenesis was synchronized in these testicular sac. In the case of spermatogonium, the nucleus was comparatively large ellipsoidal, and the nucleolus and mitochondria showed a marked development. The size of primary spermatocyte was smaller than that of spermatogonia, and that of secondary spermatocyte was smaller than that of primary spermatocyte. The chromatin of spermatocyte was highly condensed according to their development. The nucleus with electron-dense was round shape. In spermiogenesis, flagella started to be formed and chromatin was more condensed. The mitochondria were rearranged along the tail. The sperm was formed by loss of cytoplasm. The head of mature sperm was long cone shape and had not acrosome. The microtubules of flagella were arranged 9+2 structure. Also, the sperm has a loop-like structure at the end of a tail.

• The Korean Journal of Zoology
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• v.21 no.3
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• pp.87-102
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• 1978

Electron microscopic studies were made to investigate changes in the fine structure of the liver, kidney and gills of Carassius carassius L. following exposure to 1 and 2.5 ppm of $HgCl_2$. The following results were obtained: 1. In the mercury-treated liver cells, an increase in the number of lysosomes were noticed. These lysosomes appeared to be of two types; round ones containing some crystalline structures and others with phagocytosed glycogen granules and mitochondria. Also observed were mitochondrial swelling where the matrix appeared less electrondense, and segregation of the nucleoli in the nucleus. 2. In the kidney, mercury treatment resulted in thickening of the basement membrane of the glomerulus, and appearance of vacuoles and cytoplasmic bodies in the proximal convoluted tubule. The vacuoles seemed to be formed from mitochondria. Nuclear shrinkage was also noticed at 2.5 ppm of $HgCl_2$. 3. Many large and small lysosomes appeared in response to mercury in the epithelial cells of the gill lamella. Also the lamellar membrane became fuzzy in appearance. 4. It can be concluded from these results that mercury-induced changes in the fine structure are associated with activation of detoxication processes and impairment of energy metabolism.

• Applied Microscopy
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• v.37 no.2
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• pp.65-72
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• 2007

The oogenesis and ultrastructure of fertilized egg envelope of false dace were investigated by light and electron microscope. The cytoplasm of false dace oogonia was basophilic and many nucleoli were located at inner side of nuclear membrane. In primary oocytes, yolk vesicles were distributed in marginal area only and egg envelope was not formed on egg outside. In secondary oocyte, the egg envelope was formed and yolk vesicles were increased than that of early stage in cytoplasm. The amount of basophilic substance was decreased. In case of matured egg, thickness of egg envelope and site of egg were increased, basophilic substance was distributed in egg envelope around only. The yolk vesicles were changed to yolk mass in accordance with development. The fertilized egg was of ellipsoidal, adhesive type and yellowish, have a single micropyle in the area of the animal pole. The fertilized egg envelope consisted of three layers, an outer adhesive layer, a middle layer consisting of 6 lamellae alternating layers and an inner electron dense layer. An outer surface of the fertilized egg envelope was arranged by adhesive fibrous structures. In conclusion, it is summarized that the oogenesis of false dace were the increase of cell size, the formation and accumulation of yolk, and decrease of basophilic intensity in cytoplasm. These ultrastructural characteristics of fertilized egg envelope from false dace can be utilized in taxonomy of teleost.

• Applied Microscopy
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• v.28 no.1
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• pp.63-71
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• 1998

The spermatozoon of Squalidus gracilis majimae is approximately $36.6{\mu}m$ in length and is characterized by a spherical nucleils with the clear chromatin, a short midpiece containing the mitochondria, and a flagellum positioned tangentially to the nucleus. An acrosome is absent as in all teleost fishes. The nucleus is about $1.9{\mu}m$ in diameter and in its periphery contains the electron-lucent chromatin distinguished from the electron-dense chromatin occupying most of the nucleus. The shallow nuclear fossa contains the proximal centriole, instead of two centrioles in deep nuclear fossa in siluroids. The proximal and distal centrioles are oriented approximately $140^{\circ}$ to each other. The mitochondria of 10 or more in number are arranged in three layers and do not surround the axoneme. The asymmetrical distribution of the mitochondria and the eccentrical position of the nucleus with regard to the tail are the general pattern of the cyprinid spermatozoa. S. gracilis majimae spermatozoa have the most mitochondria and the deepest cytoplasmic canal among cyprinid species. The flagellum lacks the lateral fins.

• Applied Microscopy
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• v.32 no.2
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• pp.121-129
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• 2002

The structure of integumentary epidermis is studied in the bastard halibut, Paralichthys olivaceus based on the light and transmission electron microscope. Epidermal layer consists of supporting cells, unicellular glands and accessory cells. The supporting cells were classified into superficial cell, intermediated cell and basal cell. Superficial cell of epidermal layer is squamous or cuboidal and the surface is covered with numerous microridges. The supporting cells are connected to another cell with membrane interdigitations and desmosmes. And tonofilaments are developed in the cortical cytoplasm. Gland cells are classified into mucous cell and club cell. By the histochemical studies of the epidermal secretions the mucous materials are identified as neutral polysaccharides. Club cell has numerous vacuoles and microfilaments in the cytoplasm. Also chloride cells are observed in the epidermis, it cytoplasm is occupied numerous mitochondria.

• Applied Microscopy
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• v.30 no.4
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• pp.403-412
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• 2000

Urechis unicinctus spermatogenic cells, sperm and spermatids, prepared from testis are investigated to identify nuclear actin using amoeba monorlonal anti-actin as the first Ab and gold particles (10 nm) conjugated mouse IgG (immunogold) as the Ab marker. The Ag-Ab reactions analyzed the localization of nuclear actin of the spermatogenic cells and the immunogold particles incorporated mainly with nuclear matrices. A few immunogold particles are merged into the acrosomes and the other architectures of spermatogenic cells, such as mitochondrion and centrioles. It is often observed and there is a tendency in which the incorporated immunogold particles are increased in number in the nuclear matrices of sperm compared with that of spermatids The increments and decrements of the incorporated immunogold particles according to developmental stages and the spermatogenic architec-tures are interpreted and discussed in aspect of acrosomal function and of nuclear condensation of spermatids.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2009.05a
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• pp.192-193
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• 2009

CrN 코팅막은 고온에서 치밀한 Cr2O3 확산방지막을 형성함으로 $800^{\circ}C$까지 기계적성질을 유지할 수 있다. 본 실험에서는 Ar, N2, 그리고 $O_2$ 가스 분위기에서 AIP(Arc Ion Plating) 기법에 의해 다양한 조성의 Cr-O-N 박막을 Si(200)과 AISI 304 기판 위에 증착되었다. Cr-O-N 코팅막은 47.4at% 미만의 산소함량을 포함 할 때까지 B1구조를 유지하였고 코팅막 내 산소함량 24.6at%에서는 강한 XRD peak intensification을 나타내었다. 47.4at%에서는 결정상을 전혀 찾아볼 수 없는 전이구조를 나타내었고, 그 이상의 산소함량에서는 Cr22O3 결정상을 나타내었다. Cr-O(17at%)-N 조성의 코팅막에서는 (200)배향의 Grain 크기 증가 및 압축잔류응력이 증가하였으나, 그 이상의 산소함량에서는 점차 감소하였다. Cr-O(24.6at%)-N 조성의 코팅막이 가장 높은 경도를 나타내었고, 산소함량이 증가할수록 점차 향상된 마찰특성을 보였다.

• Applied Microscopy
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• v.29 no.1
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• pp.125-136
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• 1999

The ultrastructure of glandular trichomes in Pelargonium peltatum has been studied with a light microscope, transmission, and scanning electron microscope. Two types of the glands, long-stalked and short-stalked capitate glands, are distinguished with their shape and size of the total glands. Both glands are extreamly abundant in the leaf veins and petioles. These glandular trichomes are consisted of one secretory cell, three stalk cells, and one basal cell. The secretory cells contain a large amount of smooth endoplasmic reticulum. They have also much plastids, vacuoles, Golgi apparati, and mitochondria. High electron-dense deposits are frequently present in vacuoles of secretory cells. It seems to be phenolic compounds which is thought as the major secretory precursors.

• Applied Microscopy
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• v.31 no.4
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• pp.375-383
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• 2001

To understand the fine structure of Korean bat livers , the authors investigated the liver of four Korean bats; Rhinolephus ferrumequinum korai, Myotis macrodactylus, Myotis dauhentonii ussrinesis, and Murina leucogaster intermedia by transmission electron microscopy. The hepatocytes of Rhinolophus ferrumequinum korai had large-sized mitochondria and many peroxisomes. In the Myotis macrodactylus, juctional completes, especially desmosomes, were well developed. The Myotis daubentonii ussrinesis had many glycogen particles in the cytoplasm. Also, the space of Disse and sinusoidal spare was filled with amorphous materials. In the Murina leucogaster intemedia, basement membrane was prominent in the sinusoid, and no Kupffer and Ito cells were observed These results suggest the characteristic differences in the liver ultrastructure among Korean bats.