Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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An Ultrastructural Study on the Spermatogenesis of Xiphophorus maculatus

Xiphophorus maculatus의 정자형성과정에 관한 미세구조

  • Kim, Dong-Heui (Dept. of Basic Science and Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University) ;
  • Reu, Dong-Suck (Dept. of Biology, Chongju University) ;
  • Deung, Young-Kun (Dept. of Basic Science and Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University)
  • 김동희 (연세대학교 원주의과대학 기초과학교실.연세대학교 기초의학연구소) ;
  • 류동석 (청주대학교 생물학과) ;
  • 등영건 (연세대학교 원주의과대학 기초과학교실.연세대학교 기초의학연구소)
  • Published : 2003.12.01
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Abstract

The ultrastructures of spermatogenesis and sperm in Xiphophorus maculatus, ovoviviparous fish were investigated by electronmicroscopy The testis of Xiphophorus maculatus contained numerous testicular sacs, and spermatogenesis was synchronized in these testicular sac. In the case of spermatogonium, the nucleus was comparatively large ellipsoidal, and the nucleolus and mitochondria showed a marked development. The size of primary spermatocyte was smaller than that of spermatogonia, and that of secondary spermatocyte was smaller than that of primary spermatocyte. The chromatin of spermatocyte was highly condensed according to their development. The nucleus with electron-dense was round shape. In spermiogenesis, flagella started to be formed and chromatin was more condensed. The mitochondria were rearranged along the tail. The sperm was formed by loss of cytoplasm. The head of mature sperm was long cone shape and had not acrosome. The microtubules of flagella were arranged 9+2 structure. Also, the sperm has a loop-like structure at the end of a tail.

난태생 어류인 성숙한 Xiphophorus maculatus의 정소를 적출하여 정자형성과정과 정자의 미세구조를 전자현미경으로 관찰하였다. 정자형성과정은 정소낭(testicular cyst)에서 이루어지며, 각 정소낭 내에 동일한 분화시기의 생식세포가 분포하고 있었다. 정원세포는 타원형으로 핵 내에 뚜렷한 인을 보유하고 있었고 세포질에는 미토콘드리아의 발달이 현저하였다. 제1정모세포는 원형으로 정원세포보다 크기가 작았고 인은 발달되어 있지 않았으며, 제2정모세포는 제1정모세포보다 더 작아졌고 핵의 전자밀도는 더 높았다. 정세포의 초기발달시기에는 세포의 크기가 정모세포보다 작았고 염색질의 응축은 핵막 쪽에서 뚜렷하여 전자밀도가 높았으며 편모가 형성되기 시작하였고 미토콘드리아는 핵 주변에 분포하였다. 정자완성과정 중기에는 핵의 염색질 응축이 뚜렷하였으며 핵은 세포질 한쪽에 치우쳐 있었고, 미토콘드리아는 편모 주변에 집중되었으며 핵은 구형을 이루고 있었다. 완전히 성숙한 정자의 두부형태는 장원추형이었고 두부에서 첨체는 관찰되지 않았으며 편모의 미세소관 배열은 9+2구조를 이루고 있었다. 또한 정자의 꼬리 끝에는 고리형태의 구조물 보유하고 있었다.

Keywords

References

  1. AlZelius BA: Fine structure of the garfish spermatozoon, J Ultrastr Res 64: 309 314,1978 https://doi.org/10.1016/S0022-5320(78)90039-4
  2. Billard R: Spermiogenesis in the rainbow trout (Salmo gairdneri) An ultrastructural study, Cell Tissue Res 223 : 265 284, 1983
  3. Brusle S: Ultrastructure of Spermiogenesis in Liza aurata Risso, 1810 (Teleostei, MugilidaB), Cell Tissue Res 217: 415 424,1981
  4. Deung YK, Kim WJ, Kim DH, Song SB, Reu DS: An ultra-structual study on the spermatogenesis of the zebrafish (Brachydanio rerio), J Wonju College of Medicine 6(1): 186 194,1993, (Korean)
  5. Kim DH, Deung YK, Kim WJ, Reu DS, Kang SJ: Compara-tive ultrastructures of the fertilized egg envelopes from three spot gourami, pearl gourami and marble gourami, Belontiidae, Teleost. Korean J Electron Microscopy 29(3) : 343 351,1999, (Korean)
  6. Deurs BY, Lastein U: Ultrastructure of the spermatozoa of the teleost Pantodon buchholzi Peters, with particular ref-erence to the midpiece, J Ultrastr Res 42 : 517 533, 1973 https://doi.org/10.1016/S0022-5320(73)80024-3
  7. Grier HJ: Ultrastructure of the testis in the teleost Poecilia latipinna, J Ultrastr Res 45 : 82 92, 1973 https://doi.org/10.1016/S0022-5320(73)90034-8
  8. Gwo JC, Gwo HH: Spermatogenesis in the black porgy, Acanthopagrus schlegeli (Teleostei : Perciformaes : Sparidae), Molecu Reprod Dev 36 : 75 83, 1993 https://doi.org/10.1002/mrd.1080360111
  9. Hourigan TF, Nalcamura M, Nagahama Y, Yamauchi K, Grau EG: Histology, ultrastructure, and in vitro viteroidogenesis of the testes of two male phenotypes of the protogynolls Fish, Thalassoma duperrey (Labridae), General and Comparative Endocrinology 83: 193 217,1991 https://doi.org/10.1016/0016-6480(91)90023-Y
  10. Jones PR, Butler RD: Spermatozoon ultrastructure of Platichthysjlesus. J Ultrastr Molecu Struc Res 98 : 71 82, 1988 https://doi.org/10.1016/S0889-1605(88)80935-2
  11. Kessel RG, Beams HW, Tung HN, Roberts R: Unusual particle arrays in the plasma membrane of zebrafish spermatozoa, J Ultrastr Res 84 : 268 274,1983 https://doi.org/10.1016/S0022-5320(83)80006-9
  12. Marcaillou C, Annette S: The 'Blood Testis' barrier in a nematode and a fish: A generalizable concept. J Ultrastr Res70: 128 136,1980 https://doi.org/10.1016/S0022-5320(80)90028-3
  13. Medina A, Megina C, Abascal FJ, Calzada A: The sperm ultrastructure of Merluccius merluccius (Teleostei, Gadifonnes): phylogenetic considerations. Acta Zoologica 84(2) : 131 137,2003 https://doi.org/10.1046/j.1463-6395.2003.00138.x
  14. Miura T, Yamauchi K, Nagahama Y, Takahashi H: Introduction of perm atogene sis in male Japanese eel, Anguilla japonica, by a single injection of human chorionic gona-dotropin, Zool Sci 8 : 63 73,1991
  15. Rossouw GJ, Vanessen LD: Spermatogenesis in the male white spotted hOWldshark, Mustelus palubes Smith, 1957, South African J Sci 89: 244 246, 1993
  16. Rossouw GJ, Vanessen LD: Spermatogenesis in the male white spotted houndshark, Mustelus palubes Smith, 1957, South African J Sci 89: 244 246, 1993
  17. Todd PR: Ultrastructure of the spermatozoa and spermiogenesis in New Zealand freshwater eels (Anguillidae), Cell Tissue Res 171 : 224 232, 1976
  18. Wolenski JS, Hart NH: Scanning electron microscopic studies of sperm incorporation into the zebrafish (Brachydanio) egg, J Exp Zool 243: 259 273,1987 https://doi.org/10.1002/jez.1402430211
  19. Zirkin BR: The ultrastructure of nuclear differentiation during spermiogenesis in the salmon, J Ultrastr Res 50 : 174 184,1975 https://doi.org/10.1016/S0022-5320(75)80048-7