• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.10-10
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• 2018

미생물 및 식물 등이 가지고 있는 많은 천연물질은 항균, 항암, 강장작용 등 다양한 생리활성 기능이 있다고 알려져 있으며, 의약품의 원료로도 많이 이용되고 있다. 또한, 미생물을 통한 발효는 거대분자를 낮은 분자량의 물질로 분해하여 인체의 흡수를 용이하게 할 수 있을 뿐만 아니라 미생물이 자체적으로 합성하는 다양한 대사산물과 발효과정에서 기존 물질에 다양한 잔기를 가감함으로서 새로운 물질이 만들어질 수 있는 장점이 있다. 본 연구에서는 구절초의 추출물을 바탕으로 발효를 진행하여 항산화 활성 및 항비만 활성 등을 발효 전 후로 비교하여 분석하고 이를 통하여 구절초 추출물을 활용한 발효제품 개발을 추진하였다. 구절초 잎과 전초의 항산화활성 측정 결과 전반적으로 구절초 전초보다는 잎 추출물에서 항산화 활성이 높게 나타났으며 구절초 잎을 70% 에탄올로 추출한 추출물에서는 비타민C보다 3.93% 항산화 활성이 높게 나타났다. 구절초 발효액의 항산화 활성 측정 결과 발효하지 않은 시료보다 발효한 시료에서 항산화 활성이 더 높게 나타났다. 더욱이, 항비만 활성과 추출물의 혈중콜레스테롤 저감 활성도 발효추출물에서 유의하게 증가하였다. 이러한 결과는 구절초 발효 추출물이 구절초 추출물에 비하여 기능성 활성이 향상되었음을 나타내고 있으며, 이러한 결과를 바탕으로 건강기능성음료 등 다양한 제품으로의 응용이 가능할 수 있음을 시사한다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.4
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• pp.470-475
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• 2007

Antibacterial activities of Scutellaria baicalensis Georgi (SBG) extract were examined against spoilage bacteria isolated from commercial tofu and various pathogens such as Listeria monocytogenes ATCC 19115, Pseudomonas fluorescens ATCC 21541. Staphylococcus aureus ATCC 29737, Salmonella Typhimurium ATCC 11806, Vibrio parahaemolyticus ATCC 17802, and Aeromonas hydrophila KCTC 2358. Four kinds of spore forming organisms were isolated from commercial tofu and identified as Bacillus sp. KN-4, Bacillus sp. KN-6, Bacillus sp. KN-10 and Bacillus sp. KN-20 using API CHB kit. The SBG extract showed high antibacterial activities and significantly inhibited the growth of the isolated spoilage bacteria and pathogens. The inhibitory effects against the organisms increased as the concentration of the SBG extract increased. The antimicrobial activities of the SBG extract were maintained markedly after heat treatments $(80^{\circ}C/30\;min,\;100^{\circ}C/30\;min\;and\;121^{\circ}C/15min)$. The minimum inhibitory concentrations (MIC) of the SBG extract against the organisms ranged from 1,000 ppm to 5,000 ppm.

• 한국석유지질학회:학술대회논문집
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• autumn
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• pp.68-84
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• 1999

Biodegradation is a natural weathering process by microorganisms to decompose spilled oil or environmental contaminants. To accelerate this process, applying nutrients (fertilizer) or more microorganisms to naturally occurring microorganisms is called 'Bioremediation.' Presently, most popular response technique to spilled oil is mechanical cleanup using booms or skimmers. For the alternative to this technique, chemical dispersants, in-situ burning are used. Another promising alternative is bioremediation and it can clean oil contaminated seashore during enough time. In this paper, types of bioremediation technologies, its usage potential, and important consideration issues when applying this technique were summarized.

• Journal of Aquaculture
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• v.15 no.4
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• pp.261-266
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• 2002

The effluent sediment from the land-based seawater fish farms of Jeju consists of proteins, fats, ash and moisture. An evaluation of the effluent sediment as substrate for growth of phototrophic or lactic acid bacteria revealed that the sediment supported the growth of phototrophic bacteria but could support lactic acid bacteria only on supplementation with sugar. The possibility of using phototrophic bacteria for recycling the land-based seawater fish farm effluent is shown.

• Journal of Conservation Science
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• v.34 no.6
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• pp.443-458
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• 2018

An archive is a collection of documents or records. Currently, most archived documents are made of paper. Paper is susceptible to biological damage and deterioration due to its material properties. To control the biological damage, treatment with chemical disinfectants and control of the storage environment are often used. In government-controlled bulk public archives, all documents are chemically sterilized before storage. However, an extremely large quantity of public records have been produced, and storage space and conservation management are gradually reaching their limits. In this study, 60 species of microbes were identified using a genetic method. We successfully applied the adenosine triphosphate (ATP) bioluminescence method to detect microbial contamination on paper documents. A calibration curve of the ATP bioluminescence as a function of the microbe quantity was obtained, and the microbial activity on non-sterilized paper archives from 1951 was analyzed using an ATP luminometer. It was found that the microbial activity was suppressed or reduced in climate-controlled storage environments at $22^{\circ}C$ and 55% relative humidity. We anticipate that these results will be used to establish selective sterilization systems for government-controlled bulk public archives.

• The Microorganisms and Industry
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• v.14 no.3
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• pp.41-46
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• 1988

극한환경을 구성하는 중요한 환경인자는 온도, 수소이온농도, 염도, 압력, 물, 영양물질 등이 있는데 본 총설에서는 그동안 비교적 많이 다루어지지 않았던 알칼리성환경, 건조환경, 저영양환경, 심해환경과 같은 극한환경과 아울러 특수환경인 해양환경, 지하환경 등으로 나누어 설명했다. 고염환경과 고영양환경, 혐기성환경은 다른 저자에 의해 자세히 다루어지고 고온환경, 산성환경, 극지환경 등은 지면상 이유로 제외했다.

• Journal of Korean Society of Archives and Records Management
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• v.1 no.1
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• pp.231-243
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• 2001

The sterilization method of records by fumigation using mixed gas of methyl bromide(MB) and ethylene oxide(ETO) has been generally used as a way to protect biological deterioration by microorganisms and insects. In this study, we confirmed sterilization effect of MB and ETO [86 : 14(Vol.%)] on and analyzed toxicity on microorganisms isolated from records. To analyze sterilization effect of fumigation on microorganisms and insect, we have fumigated microorganisms and insect with a various amounts mixed gas of MB and ETO, and various exposure time. Insect was sterilized at all experimental conditions. In microorganisms, sterilization effect was detected only when the mixed gas was treated at $120g/m^3$ concentration for at least 24 hrs. To test the possibility of isolated microorganisms as a threat to human health, it was investigated that toxicity test using yeast, radish and cancer cells on microorganisms. Only Aspergillus oryzae had an inhibition effect on growth of yeast. radish and cancer cells, respectively. These results demonstrate that sterilization effect can occur at low concentrations of the mixed gas on insect but requires higher concentrations of the mixed gas on microorganisms. In addition to, it is suspected that the possibility of the microorganism as a threat to human health is little.

• Journal of the Korea institute for structural maintenance and inspection
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• v.27 no.1
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• pp.103-108
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• 2023

In this study, basic research was conducted to secure microbial resources applicable to autonomous crack healing concrete. To this end, in this experiment, biomineral-forming microorganisms were separated from natural sources, and the ability of survival in cement and calcium carbonate precipitation were compared to secure suitable microbial resources. Bacillus-type bacteria forming endospores were isolated from the sample, and the amount of calcium carbonate produced by the six microorganisms identified by 16S rRNA sequencing was compared. Two types of microorganisms, Bacillus velezensis and Bacillus subtilis, with the highest calcium carbonate precipitation were selected, and the survival of the microorganisms was confirmed through phase contrast microscopy after being cured after being added to the mortar. In addition, it was confirmed that the autonomous crack healing capability by the crack healing material produced by microorganisms was confirmed by artificially generating cracks in the mortar.

• Korean Journal of Microbiology
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• v.52 no.3
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• pp.375-379
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• 2016

In this study, the structure and diversity of bacterial community were investigated in the surface and subsurface marine sediments using a NGS method (i.e. illumina sequencing technology). The bacterial community in the surface was distinct from that in the subsurface of marine sediment; with the exception of the phylum Proteobacteria, the relative abundance of Bacteroides phylum were higher in the surface than subsurface, whereas the sequences affiliated to the phyla Chloroflexi and Acidobacteria were relatively more copious in the subsurface than surface sediment. Moreover, interestingly, we observed that the phyla Nitrospinae and Nitrospirae contribute to nitrogen cycle in the marine sediment. This study may present the possibility for the presence of novel microorganisms as unexplored sources and provide basic information on the microbial community structure.

• Journal of the Korea Concrete Institute
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• v.22 no.4
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• pp.547-557
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• 2010

This paper presents a study on the development of next generation smart concrete in an eco-friendly manner using micro-biologically induced calcite precipitation (MICP) via microbial biomineralization. It seems that currently, the reformation and functional improvement of concrete using MICP can be achieved using Sporosarcina pasteurii, which is a representative microorganism that produces calcite precipitation. Based on previous studies on MICP the biochemical tests and crystallinity evaluation of cement using sporoasrcina pasteurii and four additional micro-organisms from the concrete structures as identified by 16S rDNA sequence analysis were conducted. Also by applying the Sporosarcina pasteurii and separated four effective micro-organisms from the concrete structures to mortar, the compressive strength improvement by varying curing conditions, repair of crack were examined, and plans for future study were suggested. The effect of the application of effective micro-organisms can lead to the development of a new material that will contribute to resolution of environmental problems and facilitate repair work, and this can also serve as a new research theme in the future. In addition, the importance of this study is to use micro-organism, which is found common in concrete structures, this new microbial is not only environmentally safe but also persists in the natural environment for an extended period of time. Therefore, it seems to have a great potential to became a new environmentally low-burdened functional material.