• Journal of Applied Biological Chemistry
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• v.64 no.3
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• pp.245-251
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• 2021

Codonopsis lanceolata (Deoduk) was grown in East Asia, including Korea, China, Japan, and Russia, and the roots of C. lanceolata have been used as functional foods and traditional medicine to treat symptoms of cough, bronchitis, asthma, tuberculosis, and dyspepsia. The phytochemicals of C. lanceolata have been reported such as phenylpropanoids, polyacetylenes, saponins, and flavonoids that are involved in pharmacological effects such as anti-obesity, anti-inflammation, anti-tumor, anti-oxidant, and anti-microbial activities. Selecting marker substances of the main producing area by MS-based metabolomics analysis is important to ensure the beneficial effect of C. lanceolata without side-effects because differences in cultivated areas of plants were related not only to the safety of medicinal plants but also to changes in chemical composition and biological efficacy. In our present study, ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry combined with multivariate statistical analysis was applied to recognize the main producing area of C. lanceolata in South Korea. As a result of Principal Component Analysis and loading plot analysis of three groups, Inje (Kangwon-do), Hoengseong (Kangwon-do), and Muju (Jeonlabuk-do), several secondary metabolites of C. lanceolata including tangshenoside I, lancemaside A, and lancemaside G, were suggested as potential marker substances to distinguish the place of main producing area of C. lanceolata.

• Korean Journal of Remote Sensing
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• v.37 no.5_3
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• pp.1425-1434
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• 2021

Continuous and periodic data acquisition must be preceded to maintain and manage the river facilities effectively. Adapting the existing general facilities methods, which include river surveying methods such as terrestrial laser scanners, total stations, and Global Navigation Satellite System (GNSS), has limitation in terms of its costs, manpower, and times to acquire spatial information since the river facilities are distributed across the wide and long area. On the other hand, the Mobile Mapping System (MMS) has comparative advantage in acquiring the data of river facilities since it constructs three-dimensional spatial information while moving. By using the MMS, 184,646,009 points could be attained for Anyang stream with a length of 4 kilometers only in 20 minutes. Levee points were divided at intervals of 10 meters so that about 378 levee cross sections were generated. In addition, the waterside maximum and average slope could be automatically calculated by separating slope plane form levee point cloud, and the accuracy of RMSE was confirmed by comparing with manually calculated slope. The reference slope was calculated manually by plotting point cloud of levee slope plane and selecting two points that use location information when calculating the slope. Also, as a result of comparing the water side slope with slope standard in basic river plan for Anyang stream, it is confirmed that inspecting the river facilities with the MMS point cloud is highly recommended than the existing river survey.

• Journal of Mushroom
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• v.19 no.3
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• pp.140-149
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• 2021

Mushroom-derived polysaccharides, which are the primary bioactive constituents, are beneficial for human health. Polysaccharides have immuno-modulation, antitumor, and antioxidant properties. Additionally, they have antiviral properties and protect against chronic radiation stress. In this study, high yield water-soluble polysaccharides were obtained from Pleurotus djamor var. roseus basidiocarps. The crude polysaccharide (CP) was extracted sequentially by hot water and ethanol precipitation. The yield of the brown CPs was 5.6% dw. Diethylaminoethyl cellulose and Sepharose-6B column chromatography of CPs generated several fractions. Total glucan content was determined in all the fractions. The F1 fraction displayed the highest sugar content and was considered as a purified polysaccharide (PP). The total glucan and β-glucan content in the four fractions ranged between 76.85-2.95% and 75.08-1.46%, respectively. The yield of the PPs was 300 mg, and it was obtained as a white powder. The PPs were characterized by Fourier-transform infrared spectroscopy (FTIR) and thin-layer chromatography. The FTIR spectral details confirmed the presence of a xylopentose polysaccharide. The antioxidant activity of the PPs was evaluated using in vitro 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay and superoxide radical scavenging assay. The PPs showed strong DPPH free radical and superoxide anion radical scavenging activities in a dose-dependent manner. Purified PPs free of phenolics, protein, and carbohydrates were mainly responsible for the radical scavenging activity. The data suggest the potential of PPs as natural antioxidants.

• Korean Journal of Environment and Ecology
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• v.35 no.5
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• pp.503-524
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• 2021

A largemouth bass (Micropterus salmoides) is an ecosystem disturbance fish species at the highest rank in the aquatic ecosystem, causing a serious imbalance in freshwater ecosystems. Although various attempts have been made to eradicate and control largemouth bass, no effective measures were found. Therefore, it is necessary to find an approach to maximize the effective population reduction based on the unique characteristics of largemouth bass. This study used the transcriptome analysis to derive 182,887 unigene contigs and select 12 types of final target sequences for applying the CRISPR/Cas9 system in the genes of IZUMO1 and Zona pellucida sperm-binding protein, which are proteins involved in sperm-egg recognition. After synthesizing 12 types of sgRNA capable of recognizing each target sequence, 12 types of Cas9-sgRNA ribonucleoprotein (RNP) complexes to be used in subsequent studies were prepared. This study searched the protein-coding gene of sperm-egg through the Next Generation Sequencing (NGS) and edited genes through the CRISPR/Cas9 system to induce infertile individuals that produced reproductive cells but could not form fertilized eggs. Through such a series of processes, it successfully established a composition development process for largemouth bass. It is judged that this study contributed to securing the valuable basic data for follow-up studies to verify its effect for the management of ecological disturbances without affecting the habitat of other endemic species in the same water system with the largemouth bass.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.41 no.5
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• pp.551-557
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• 2021

Attached parking lots installation criteria is determined by use, total floor area, etc. according to the Parking Lot Act and local government ordinances. However, with traffic demand increase inconvenience about use of culture and assembly facilities have been raised. When planning number of parking lots for cultural and assembly facilities, legal parking lots and unit parking lots are used, but this causes inconvenience and traffic problems on the surrounding roads, because reality and convenience are not considered. Therefore, this study intend to present an realistic number of parking lots calculating equation for movie theater in Gwangju Metropolitan City. After investigating number of parking lots, number of screens, number of seats, total floor area, bus route and illegal parking for a cultural facility in Gwangju Metropolitan City, prediction model for calculating number of parking lots was presented using SPSS regression analysis. As a result of comparing prediction model and unit method, the prediction model was be closer actual cumulative parking space, so prediction model verification was completed. Based on the model verified in this study, Realistic number of parking lots will be installed. However, due to limitations of research on specific areas, research on various facilities should continue in consideration of regional, population, and urban characteristics

• Journal of Dental Rehabilitation and Applied Science
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• v.37 no.3
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• pp.171-176
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• 2021

Pneumomediastinum is a very rare and potentially catastrophic complication of dental procedures. Its common causes are tooth extraction, endodontic treatment, and subgingival curettage using handpieces and high-pressure air/water syringes. We present a case of massive pneumomediastinum with subcutaneous emphysema in a 61-year-old female who underwent bone grafting into the maxilla for pretreatment of dental implantation using a syringe. The patient suffered from abrupt severe odynophagia and loss of consciousness. The patient transferred to emergency department and images work-up revealed a pneumomediastinum and subcutaneous emphysema on the entire face and neck. We performed conservative treatments including prophylactic antibiotics, oxygen inhalation, and fasting meals, and then discharge after 7 days uneventfully. The patient's syncope might be resulting from hypotension and pain shock induced by pneumomediastinum with a sudden chest compression. The pneumomediastinum could be resulting from concurrent perforation and massive air infiltration into the maxillary sinus during bone grafting. We suggest that pneumomediastinum needs prompt diagnosis and management because of the risk of airway obstruction when a patient present syncope in the dental room.

• Clean Technology
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• v.27 no.3
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• pp.247-254
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• 2021

In this study, an eco-friendly combustion process of waste 2,4,6-trinitrotoluene (TNT: 2,4,6-trinitrotoluene) was developed, and fundamental data for the quantity of the organic matter in the final combustion residues is presented. Because complete combustion of TNT is not possible theoretically, the combustion process was optimized to reduce organic matter content in the combustion residue by performing measures such as heating time changes, addition of propellant material, and after treatment using a high-temp electrical furnace. From the results, it was confirmed that the organic matter content in the residue could be decreased to 7 ~ 10% with each method. The quantity of the organic matter could be minimized by optimizing the combustion conditions of the process. With only a combustion time increase, the amount of organic matter in the combustion residues was measured at about 9 wt%. The environmental friendliness of the final exhaust gas was also confirmed by real time gas component analyses. In addition, the organic contents could be reduced by a further 2 wt% by applying an additional heat treatment using an external electric furnace after the first incineration treatment. In the combustion process of propellant added waste TNT, it was found that various TNT wastes could be treated using the same eco-friendly protocols because the organic content in the residue decreased in accordance with the amount of propellant. The amount of the organic matter content produced by all these methods fulfilled the requirements under the Waste Management Act.

• The Journal of Korean Academy of Prosthodontics
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• v.59 no.2
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• pp.190-200
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• 2021

Purpose. The aim of this study was to evaluate biaxial flexural strength and hardness of colored monolithic zirconia after dipping in different time intervals of coloring solution. Materials and Methods. Disk shaped specimens were prepared from monolithic zirconia (Eclipse V2.0, AMS, Gimpo, Korea). Four experimental groups were categorized (n = 12) due to coloring time (PU (0s); ST (8s); OV (1 min); PS (preshade)), to evaluate biaxial flexural strength and Vickers hardness. After fracture, X-ray diffraction analysis was performed using fractured specimens. Results were analyzed with one-way ANOVA test. Results. There was no significant difference between groups in the biaxial flexural strength test. However, in the Vickers hardness test, the group with standard dipping time (ST) showed significantly higher value than the group without dipping in coloring liquid (PU)(P=.038). Also, there was no significant difference in the rest of the groups (P>.05). As a result of X-ray diffraction analysis, specific peaks of tetragonal phase were shown and the volume of monoclinic phase fraction was lower than 25%. Conclusion. Although this study has several limitations, coloring liquids had no significant effect on biaxial flexural strength. Vickers hardness was significantly different between the group to which the coloring liquid was applied and the group to which the coloring solution was not applied, but there was no significant difference between the other groups. Also, the flexural strength of monolithic zirconia corresponds to Class 5 of the minimal flexural strength standard according to the use of dental ceramics.

• Journal of Internet Computing and Services
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• v.22 no.1
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• pp.31-39
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• 2021

Obtaining delivery addresses from calls is one of the most important processes in TV home shopping business. By automating this process, the operational efficiency of TV home shopping can be increased. In this paper, a method of recognizing and validating road name address, which is the address system of South Korea, from speech oriented text is proposed. The speech oriented text has three challenges. The first is that the numbers are represented in the form of pronunciation. The second is that the recorded address has noises that are made from repeated pronunciation of the same address, or unordered address. The third is that the readability of the resulted address. For resolving these problems, the proposed method enhances the existing address databases provided by the Korea Post and Ministry of the Interior and Safety. Various types of pronouncing address are added, and heuristic rules for dividing ambiguous pronunciations are employed. And the processed address is validated by checking the existence in the official address database. Even though, this proposed method is for the STT result of the address pronunciation, this also can be used for any 3^rd party services that need to validate road name address. The proposed method works robustly on noises such as positions change or omission of elements.

• Korea Science and Art Forum
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• v.37 no.2
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• pp.101-112
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• 2019

Regardless of the size, the role and meaning of the space required for human daily life are the same. Especially, as the spatial design is small and the size is small, the careful design and problem solving are needed to enable comfortable and convenient life even in a narrow space. The purpose of this study is to design a convergence model that utilizes the advantages of BIM, which can simulate actual design based on a new one-person housing space design plan optimized for one person. This study applied the BIM design technology to one-person housing 2D design, and the suitability examination and the space optimization design of the interior design were carried out. As a result of the study, utility of space improvement, consideration of housing environment, interference check, application of eco-friendly housing system, and MEP design item were derived. Therefore, BIM space design in interior space has been confirmed as a way to overcome limit and inefficiency of 2D design which is applied to actual space by various space design elements. Based on the results of this study, the One-person housing space model, which is applied to the study, is a pure creation designed based on various one-person housing of social and cultural peculiarities derived from previous research. This design example was applied to BIM technology to confirm the detailed and practical design possibility.